ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0131 suspect: Pn R KOG0118 General function prediction only FOG: RRM domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0131 46483 45758 -242
(242 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.11 [R] KOG0118 FOG: RRM domain 56 4e-08
At3g52380 [R] KOG0118 FOG: RRM domain 55 9e-08
At4g24770 [R] KOG0118 FOG: RRM domain 53 3e-07
At5g50250 [R] KOG0118 FOG: RRM domain 52 1e-06
HsM18152777 [R] KOG0118 FOG: RRM domain 50 4e-06
At1g49760 [AJ] KOG0123 Polyadenylate-binding protein (RRM superf... 49 6e-06
YNL004w [R] KOG0118 FOG: RRM domain 49 8e-06
At4g26650 [A] KOG4205 RNA-binding protein musashi/mRNA cleavage ... 49 8e-06
>SPBC660.11 [R] KOG0118 FOG: RRM domain
Length = 348
Score = 56.2 bits (134), Expect = 4e-08
Identities = 62/279 (22%), Positives = 104/279 (37%), Gaps = 55/279 (19%)
Query: 19 IFVGNLSPETSPEDLQKVF---GESVKVEIP---TLQSDRTYPRIFAFVTFDDKVDVEDL 72
+FVG LS T +++ +F G KV IP + R P AFVTF+++ DV+
Sbjct: 46 VFVGRLSTSTKKSEIRSLFETVGTVRKVTIPFRRVRRGTRLVPSGIAFVTFNNQEDVDKA 105
Query: 73 RSKFDKTVIKDKSIYVTKVLTPEEQQLKKQKRRANQRGKA-------------------- 112
+ + D+ I V K +EQ +K +K+ N+ G+
Sbjct: 106 IETLNGKTLDDREIVVQKARPVQEQPIKDRKKSKNKNGEEPETSTSVENAESAKGSSDEN 165
Query: 113 ---VPAPPKKNKEAQVPLEQME--------RSK------DTLYVNNIPYHTTKAEIASFF 155
P N+ V +Q E ++K +++YV+ + T + F
Sbjct: 166 EANTATAPSSNEANGVDKKQNEIKGKGGSGKNKAKPLPPNSIYVSGLSVTLTNEGLKEMF 225
Query: 156 GTTEES-VILPMRRMKDTTTKRVFF--SRKFNRGIAFVSFPEGTDIEAKAAEFNGKNFED 212
+ + +R + +R+ ++ RG FVSF D E NGK D
Sbjct: 226 DAYNPTRARIAVRSLPPYIIRRIKLRGEQRRGRGFGFVSFANAEDQSRAIEEMNGKQVGD 285
Query: 213 RELTVDVAA---------NKPAHTEPVQTEAEHGNSTSE 242
L V A N+ EP++ + S+
Sbjct: 286 LTLVVKSAVFREDKQNDENEKNENEPIEASESAPTNVSD 324
Score = 53.5 bits (127), Expect = 3e-07
Identities = 36/124 (29%), Positives = 59/124 (47%), Gaps = 13/124 (10%)
Query: 121 KEAQVPLEQMERSKDTLYVNNIPYHTTKAEIASFFGT--TEESVILPMRRMKDTTTKRVF 178
+++ P +Q + + ++V + T K+EI S F T T V +P RR++ T
Sbjct: 32 EQSNTPAQQADDFR--VFVGRLSTSTKKSEIRSLFETVGTVRKVTIPFRRVRRGT----- 84
Query: 179 FSRKFNRGIAFVSFPEGTDIEAKAAEFNGKNFEDRELTVDVAANKPAHTEPVQTEAEHGN 238
R GIAFV+F D++ NGK +DRE+ V A +P +P++ + N
Sbjct: 85 --RLVPSGIAFVTFNNQEDVDKAIETLNGKTLDDREIVVQKA--RPVQEQPIKDRKKSKN 140
Query: 239 STSE 242
E
Sbjct: 141 KNGE 144
>At3g52380 [R] KOG0118 FOG: RRM domain
Length = 329
Score = 55.1 bits (131), Expect = 9e-08
Identities = 56/243 (23%), Positives = 101/243 (41%), Gaps = 34/243 (13%)
Query: 6 ETSTKIVVDPETSIFVGNLSPETSPEDLQKVFGESVKVEIPTLQSDRTYPRI--FAFVTF 63
E T E ++VGNL + +L ++FGE+ V + D+ R F FVT
Sbjct: 105 EKQTTQASGEEGRLYVGNLPYTITSSELSQIFGEAGTVVDVQIVYDKVTDRSRGFGFVTM 164
Query: 64 DDKVDVEDLRSKFDKTVIKDKSIYVTKVLTPE--EQQLKKQKRRANQRGKAVPAPPKKNK 121
+ ++ F+ + I +++ V P E ++ + K R N R V +P K
Sbjct: 165 GSIEEAKEAMQMFNSSQIGGRTVKVNFPEVPRGGENEVMRTKIRDNNRSY-VDSPHK--- 220
Query: 122 EAQVPLEQMERSKDTLYVNNIPYHTTKAEIASFFGTTEESVILPMRRMKDTTTKRVFFSR 181
+Y N+ ++ T + FG ++ +L + + + T R
Sbjct: 221 ---------------VYAGNLGWNLTSQGLKDAFG--DQPGVLGAKVIYERNTGR----- 258
Query: 182 KFNRGIAFVSFPEGTDIEAKAAEFNGKNFEDRELTVDVAA--NKPAHTEPVQTEAEHGNS 239
+RG F+SF ++++ A NG E R L +++A+ KP + P E E +
Sbjct: 259 --SRGFGFISFESAENVQSALATMNGVEVEGRALRLNLASEREKPTVSPPSVEEGETEEA 316
Query: 240 TSE 242
+ E
Sbjct: 317 SLE 319
>At4g24770 [R] KOG0118 FOG: RRM domain
Length = 329
Score = 53.1 bits (126), Expect = 3e-07
Identities = 47/211 (22%), Positives = 88/211 (41%), Gaps = 37/211 (17%)
Query: 16 ETSIFVGNLSPETSPEDLQKVFGESVKVEIPTLQSDRT--YPRIFAFVTFDDKVDVEDLR 73
E +FVGNL+ + + + L +F ++ VEI + +R R F FVT + E
Sbjct: 149 EAKLFVGNLAYDVNSQALAMLFEQAGTVEIAEVIYNRETDQSRGFGFVTMSSVDEAETAV 208
Query: 74 SKFDKTVIKDKSIYVTKVLTPEEQQLKKQKRRANQRGKAVPAPPKKNKEAQVPLEQMERS 133
KF++ + + + V K A P+ ++ + P ++
Sbjct: 209 EKFNRYDLNGRLLTVNK------------------------AAPRGSRPERAP--RVYEP 242
Query: 134 KDTLYVNNIPYHTTKAEIASFFGTTEESVILPMRRMKDTTTKRVFFSRKFNRGIAFVSFP 193
+YV N+P+ + F +E ++ R + D T R +RG FV+
Sbjct: 243 AFRVYVGNLPWDVDNGRLEQLF--SEHGKVVEARVVYDRETGR-------SRGFGFVTMS 293
Query: 194 EGTDIEAKAAEFNGKNFEDRELTVDVAANKP 224
+ ++ + +G+N E R + V+VA +P
Sbjct: 294 DVDELNEAISALDGQNLEGRAIRVNVAEERP 324
>At5g50250 [R] KOG0118 FOG: RRM domain
Length = 289
Score = 51.6 bits (122), Expect = 1e-06
Identities = 49/210 (23%), Positives = 78/210 (36%), Gaps = 37/210 (17%)
Query: 16 ETSIFVGNLSPETSPEDLQKVFGESVKVEIPTL--QSDRTYPRIFAFVTFDDKVDVEDLR 73
E +FVGNL + + L +F ++ VEI + D R F FVT + E
Sbjct: 112 EAKLFVGNLPYDVDSQALAMLFEQAGTVEISEVIYNRDTDQSRGFGFVTMSTVEEAEKAV 171
Query: 74 SKFDKTVIKDKSIYVTKVLTPEEQQLKKQKRRANQRGKAVPAPPKKNKEAQVPLEQMERS 133
KF+ + + + V RA RG P+ A
Sbjct: 172 EKFNSFEVNGRRLTVN---------------RAAPRGSRPERQPRVYDAAF--------- 207
Query: 134 KDTLYVNNIPYHTTKAEIASFFGTTEESVILPMRRMKDTTTKRVFFSRKFNRGIAFVSFP 193
+YV N+P+ + F +E ++ R + D T R +RG FV
Sbjct: 208 --RIYVGNLPWDVDSGRLERLF--SEHGKVVDARVVSDRETGR-------SRGFGFVQMS 256
Query: 194 EGTDIEAKAAEFNGKNFEDRELTVDVAANK 223
++ A +G+N E R + V+VA +
Sbjct: 257 NENEVNVAIAALDGQNLEGRAIKVNVAEER 286
>HsM18152777 [R] KOG0118 FOG: RRM domain
Length = 261
Score = 49.7 bits (117), Expect = 4e-06
Identities = 37/132 (28%), Positives = 63/132 (47%), Gaps = 14/132 (10%)
Query: 15 PETSIFVGNLSPETSPEDLQKVFGE---SVKVEIPTLQSDRTYPRIFAFVTFDDKVDVED 71
P TS+F+ N++ T PEDL++ FG V V IP L PR FA+V F+D D ED
Sbjct: 8 PNTSLFIRNVADATRPEDLRREFGRYGPIVDVYIP-LDFYTRRPRGFAYVQFEDVRDAED 66
Query: 72 LRSKFDKTVIKDKSIYVTKVLTPEEQ--QLKKQKRRANQRGKAVPAPPKKNKEAQVPLEQ 129
++ + + I + + Q+K ++R P P ++ ++ P ++
Sbjct: 67 ALYNLNRKWVCGRQIEIQFAQGDRKTPGQMKSKERH--------PCSPSDHRRSRSPSQR 118
Query: 130 MERSKDTLYVNN 141
RS+ + + N
Sbjct: 119 RTRSRSSSWGRN 130
>At1g49760 [AJ] KOG0123 Polyadenylate-binding protein (RRM superfamily)
Length = 704
Score = 48.9 bits (115), Expect = 6e-06
Identities = 52/219 (23%), Positives = 82/219 (36%), Gaps = 29/219 (13%)
Query: 17 TSIFVGNLSPETSPEDLQKVFGE-SVKVEIPTLQSDRTYPRIFAFVTFDDKVDVEDLRSK 75
T+++V NLS S E+L KVFGE V ++ + F FV F++ D
Sbjct: 224 TNVYVKNLSESLSDEELNKVFGEFGVTTSCVIMRDGEGKSKGFGFVNFENSDDAARAVDA 283
Query: 76 FDKTVIKDKSIYVTKVLTPEEQQLKKQKRRANQRGKAVPAPPKKNKEAQVPLEQMERSK- 134
+ DK +V K E++ + K K Q E ++S+
Sbjct: 284 LNGKTFDDKEWFVGKAQKKSERETE-----------------LKQKFEQSLKEAADKSQG 326
Query: 135 DTLYVNNIPYHTTKAEIASFFGTTEESVILPMRRMKDTTTKRVFFSRKFNRGIAFVSFPE 194
LYV N+ T ++ F I + M+D + +RG FV+F
Sbjct: 327 SNLYVKNLDESVTDDKLREHFAPF--GTITSCKVMRDPS--------GVSRGSGFVAFST 376
Query: 195 GTDIEAKAAEFNGKNFEDRELTVDVAANKPAHTEPVQTE 233
+ E NGK + L V +A K +Q +
Sbjct: 377 PEEATRAITEMNGKMIVTKPLYVALAQRKEDRKARLQAQ 415
>YNL004w [R] KOG0118 FOG: RRM domain
Length = 429
Score = 48.5 bits (114), Expect = 8e-06
Identities = 30/94 (31%), Positives = 50/94 (52%), Gaps = 2/94 (2%)
Query: 18 SIFVGNLSPETSPEDLQKVFGESVKVEIPTLQSDRTYPRIFAFVTFDDKVDVEDLRSKFD 77
SIFVGNL+ +++PEDL + F + KV + + R + R V F + DV+ ++D
Sbjct: 137 SIFVGNLTYDSTPEDLTEFFSQIGKVVRADIITSRGHHRGMGTVEFTNSDDVDRAIRQYD 196
Query: 78 KTVIKDKSIYVTKVLTPEEQQLKKQKRRANQRGK 111
D+ I+V + P +K +R+A RG+
Sbjct: 197 GAFFMDRKIFVRQDNPPPSNNIK--ERKALDRGE 228
>At4g26650 [A] KOG4205 RNA-binding protein musashi/mRNA cleavage and
polyadenylation factor I complex subunit HRP1
Length = 524
Score = 48.5 bits (114), Expect = 8e-06
Identities = 54/202 (26%), Positives = 86/202 (41%), Gaps = 34/202 (16%)
Query: 19 IFVGNLSPETSPEDLQKVFGESVKVEIPTLQSDRTYPRI--FAFVTFDDKVDVEDLRSKF 76
+F+G +S +T E LQ+ FG+ + + DRT R F F+ F D E R
Sbjct: 10 LFIGGISWDTDEERLQEYFGKYGDLVEAVIMRDRTTGRARGFGFIVFADPSVAE--RVIM 67
Query: 77 DKTVIKDKSIYVTKVLTPEEQQLKKQKRRANQRGKAVPAPPKKNKEAQVPLEQMERSKDT 136
DK +I +++ K + ++QQ+ KR A+ P+ A+
Sbjct: 68 DKHIIDGRTVEAKKAVPRDDQQV--LKRHASPMHLISPSHGGNGGGART---------KK 116
Query: 137 LYVNNIPYHTTKAEIASF---FGTTEESVILPMRRMKDTTTKRVFFSRKFNRGIAFVSF- 192
++V +P T+AE ++ FGT + V+ M D T+R RG F++F
Sbjct: 117 IFVGGLPSSITEAEFKNYFDQFGTIADVVV-----MYDHNTQR-------PRGFGFITFD 164
Query: 193 -PEGTD--IEAKAAEFNGKNFE 211
E D + E NGK E
Sbjct: 165 SEESVDMVLHKTFHELNGKMVE 186
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.129 0.351
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,288,971
Number of Sequences: 60738
Number of extensions: 606365
Number of successful extensions: 2890
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2869
Number of HSP's gapped (non-prelim): 21
length of query: 242
length of database: 30,389,216
effective HSP length: 103
effective length of query: 139
effective length of database: 24,133,202
effective search space: 3354515078
effective search space used: 3354515078
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)