ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0157 good U KOG3208 Intracellular trafficking, secretion, and vesicular transport SNARE protein GS28
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0157 55542 54898 -215
(215 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL031c [U] KOG3208 SNARE protein GS28 260 1e-69
Hs4758456 [U] KOG3208 SNARE protein GS28 80 3e-15
CE27935 [U] KOG3208 SNARE protein GS28 79 5e-15
7300422 [U] KOG3208 SNARE protein GS28 74 2e-13
At2g45200 [U] KOG3208 SNARE protein GS28 73 3e-13
At1g15880 [U] KOG3208 SNARE protein GS28 70 2e-12
SPAC4G8.10 [U] KOG3208 SNARE protein GS28 69 6e-12
>YHL031c [U] KOG3208 SNARE protein GS28
Length = 223
Score = 260 bits (664), Expect = 1e-69
Identities = 133/217 (61%), Positives = 173/217 (79%), Gaps = 6/217 (2%)
Query: 4 SFVTVRSKAISLESQTDSLLTKYSSFAQTTSSEPSGQETQLQEKLEKILNQRQETVESLQ 63
SFVT+R KAISLE+QT+SLL+KYS+FAQTTSSE +GQE ++ ++LE IL QRQ+ ++SL
Sbjct: 6 SFVTIRGKAISLETQTESLLSKYSTFAQTTSSEQTGQEKKIDKQLEGILGQRQDVIDSLT 65
Query: 64 RIADSESNISTSKLSQLQRHRETLQDNWQTFRDIRSSIQQERNRLNLLFSVKKDLQNSAS 123
+I DS IS SKLSQL RH+E LQD+W++FR+IRSSIQQERNRLNLLFSVK D+ NS +
Sbjct: 66 QICDSNPAISASKLSQLHRHKEILQDHWKSFRNIRSSIQQERNRLNLLFSVKNDIANSTT 125
Query: 124 D------NQNEYIQDEARRVENSHTVVDSLIDQAYETREQFMSQRVFLQRSSDRILQTLQ 177
D + +EYIQ+E RR++ S+ VVD LI QA+ETR QF SQ L +++++LQTLQ
Sbjct: 126 DAPAPIGDADEYIQNETRRIDQSNNVVDRLISQAWETRSQFHSQSNVLNTANNKVLQTLQ 185
Query: 178 HIPLINNVISKINTRRKKNAVILASLISICILVLFFT 214
IP +N +I KINTRRKKNA +LA++ ++CIL LFFT
Sbjct: 186 RIPGVNQLIMKINTRRKKNAFVLATITTLCILFLFFT 222
>Hs4758456 [U] KOG3208 SNARE protein GS28
Length = 250
Score = 79.7 bits (195), Expect = 3e-15
Identities = 59/243 (24%), Positives = 111/243 (45%), Gaps = 29/243 (11%)
Query: 2 SASFVTVRSKAISLESQTDSLLTKYSSF--AQTTSSEPSGQETQLQEKLEKILN------ 53
S+ + +R +A LE++ D L +S + + SS G+ + +LN
Sbjct: 6 SSYWEDLRKQARQLENELDLKLVSFSKLCTSYSHSSTRDGRRDRYSSDTTPLLNGSSQDR 65
Query: 54 -------QRQETVESLQRIADSESNISTSK---------LSQLQRHRETLQDNWQTFRDI 97
+ ++ + L + D + + S + LQRHR+ LQD F
Sbjct: 66 MFETMAIEIEQLLARLTGVNDKMAEYTNSAGVPSLNAALMHTLQRHRDILQDYTHEFHKT 125
Query: 98 RSSIQQERNRLNLLFSVKKDLQNSAS-----DNQNEYIQDEARRVENSHTVVDSLIDQAY 152
+++ R R NL+ SV+KD+++ S + + E E + NS +++ I A
Sbjct: 126 KANFMAIRERENLMGSVRKDIESYKSGSGVNNRRTELFLKEHDHLRNSDRLIEETISIAM 185
Query: 153 ETREQFMSQRVFLQRSSDRILQTLQHIPLINNVISKINTRRKKNAVILASLISICILVLF 212
T+E SQR L+ ++ P +N++I +IN R++++++IL +I IC ++L
Sbjct: 186 ATKENMTSQRGMLKSIHSKMNTLANRFPAVNSLIQRINLRKRRDSLILGGVIGICTILLL 245
Query: 213 FTA 215
A
Sbjct: 246 LYA 248
>CE27935 [U] KOG3208 SNARE protein GS28
Length = 234
Score = 79.0 bits (193), Expect = 5e-15
Identities = 61/232 (26%), Positives = 111/232 (47%), Gaps = 21/232 (9%)
Query: 1 MSASFVTVRSKAISLESQTDSLLTKYSSFA-----------QTTSSEPSGQETQLQEKLE 49
MS ++ +R KA S E+ D L + +T SS + +T E +E
Sbjct: 1 MSETWEALRKKARSTENSIDVKLVSLNKLTASSHGGFDIDEKTVSSRQTSFKTVTTE-IE 59
Query: 50 KILNQRQETVESLQRIADSESNISTSKLSQLQ----RHRETLQDNWQTFRDIRSSIQQER 105
++ Q + + +A ++S+ S + +Q RHRE L+D +R R ++ Q
Sbjct: 60 GLIEQLTNINDDMNDVAGAQSSASWANNPAIQHTLRRHREILRDYGSEYRRARDNVDQVL 119
Query: 106 NRLNLLFSVKKDLQNSASDNQ----NEYIQDEARRVENSHTVVDSLIDQAYETREQFMSQ 161
R LL S ++ N +N+ + Y++ E + ++D ++ A T+E Q
Sbjct: 120 QRELLLSSSNENRNNPILNNRARGYDMYLK-ENDHINACDRLLDEQLEMAMSTKENMARQ 178
Query: 162 RVFLQRSSDRILQTLQHIPLINNVISKINTRRKKNAVILASLISICILVLFF 213
+ L+ S R+ + P INN++ KI T+++KN +ILA++IS C++ F
Sbjct: 179 GINLRGISTRLHHISKKYPAINNLMQKIKTKKQKNTLILAAVISSCLIFTIF 230
>7300422 [U] KOG3208 SNARE protein GS28
Length = 232
Score = 73.9 bits (180), Expect = 2e-13
Identities = 55/227 (24%), Positives = 108/227 (47%), Gaps = 14/227 (6%)
Query: 3 ASFVTVRSKAISLESQTDSLLTKYSSFAQTTSSEPSGQETQL-------QEKLEKILNQR 55
+S+ +R +A SLE++ D L +S + SG + + + + +
Sbjct: 4 SSYDVLRKQARSLENEIDLKLVAFSKIGAGSGGGGSGGLGGVDTSPLLGEHVFDSLSEEI 63
Query: 56 QETVESLQRIADSESNISTS---KLSQLQRHRETLQDNWQTFRDIRSSIQQERNRLNLL- 111
++ +E L + +S S++ S + LQRHRE LQ Q F I ++ R LL
Sbjct: 64 EQMLEKLSSLNESMSDLPASGAAAMHTLQRHREILQGYRQEFNKICANHTMRIEREELLR 123
Query: 112 ---FSVKKDLQNSASDNQNEYIQDEARRVENSHTVVDSLIDQAYETREQFMSQRVFLQRS 168
+ + + N+ E E+ + ++ +V+ I+ A ETR+ +QR +R
Sbjct: 124 GSGLATSSGSPSISGLNRREMYLKESGHLNSASHLVNDQINIAIETRDHLHAQRQAFKRL 183
Query: 169 SDRILQTLQHIPLINNVISKINTRRKKNAVILASLISICILVLFFTA 215
R PLI+++I +IN +++++++IL ++I C+++L A
Sbjct: 184 QTRFNDISNRFPLISSLIQRINIKKRRDSLILGAVIGFCVILLLLYA 230
>At2g45200 [U] KOG3208 SNARE protein GS28
Length = 239
Score = 72.8 bits (177), Expect = 3e-13
Identities = 62/222 (27%), Positives = 101/222 (44%), Gaps = 23/222 (10%)
Query: 8 VRSKAISLESQTDSLLTKYSS----FAQ----TTSSEPSGQ-------ETQLQEKLEKIL 52
+R +A +E D L+ Y+ F Q T S G E ++Q LEK+L
Sbjct: 16 LRREARKIEGDLDVKLSSYAKLGARFTQGGYVDTGSPTVGSGRSWKSMEMEIQSLLEKLL 75
Query: 53 NQRQETVESLQRIADSESNISTSKLSQLQRHRETLQDNWQTFRDIRSSIQQERNRLNLLF 112
+ +S+ R A S + +TS +L RHR+ L + Q FR I+ +I R LL
Sbjct: 76 DIN----DSMSRCAASAAP-TTSVTQKLARHRDILHEYTQEFRRIKGNINSLREHAELLS 130
Query: 113 SVKKDLQN---SASDNQNEYIQDEARRVENSHTVVDSLIDQAYETREQFMSQRVFLQRSS 169
SV+ D+ S S + + E + S + +D +I QA TR SQR
Sbjct: 131 SVRDDISEYKASGSMSPGVQVLRERASIHGSISHIDDVIGQAQATRAVLGSQRSLFSDVQ 190
Query: 170 DRILQTLQHIPLINNVISKINTRRKKNAVILASLISICILVL 211
++ P+I ++ I +R ++ +IL+++I+ C L L
Sbjct: 191 GKVKNLGDKFPVIRGLLGSIKRKRSRDTLILSAVIAACTLFL 232
>At1g15880 [U] KOG3208 SNARE protein GS28
Length = 223
Score = 70.5 bits (171), Expect = 2e-12
Identities = 52/216 (24%), Positives = 104/216 (48%), Gaps = 9/216 (4%)
Query: 3 ASFVTVRSKAISLESQTDSLLTKYSSFAQTTS-SEPSGQETQLQEKLEKILNQRQETVES 61
+S+ +R +A +E+Q D + Y T + S+ G E+ L+ ++ +L Q Q+
Sbjct: 5 SSWDALRKQARKIEAQLDEQMHSYRRLVSTKALSKSDGNESDLEAGIDLLLRQLQQVNAQ 64
Query: 62 LQRIADSESNISTSKLSQLQRHRETLQDNWQTFRDIRSSIQQERNRLNLLFSVKKDLQNS 121
+Q S + S L RH+E LQD Q F RSS++ ++ +LL + + +
Sbjct: 65 MQAWVSSGGSEMVSHT--LTRHQEILQDLTQEFYRHRSSLRAKQEHASLLEDFR-EFDRT 121
Query: 122 ASDNQNEYIQDEARRVE----NSHTV-VDSLIDQAYETREQFMSQRVFLQRSSDRILQTL 176
D ++ Y ++A E N +T +D +I QA T + QR + ++
Sbjct: 122 RLDLEDGYGSEQALIKEHMGINRNTAQMDGVISQAQATLGTLVFQRSTFGGINSKLSNVA 181
Query: 177 QHIPLINNVISKINTRRKKNAVILASLISICILVLF 212
+P +N +++ I ++ + +IL+ + ++C ++F
Sbjct: 182 SRLPTVNTILAAIKRKKSMDTIILSLVAAVCTFLIF 217
>SPAC4G8.10 [U] KOG3208 SNARE protein GS28
Length = 182
Score = 68.6 bits (166), Expect = 6e-12
Identities = 51/189 (26%), Positives = 91/189 (47%), Gaps = 20/189 (10%)
Query: 25 KYSSFAQTTSSEPSGQETQLQEKLEKILNQRQETVESLQRIADSESNISTSKLSQLQRHR 84
K S F ++ S+P+ Q K+ +L +R++ +E AD + S ++ ++ H
Sbjct: 12 KASQFQRSLHSDPN------QAKI--LLEERRKLLEEANSSADENDSHS---MATIKSHF 60
Query: 85 ETLQDNWQTFRDIRSSIQQERNRLNLLFSVKKDLQNSASDNQNEYIQDEARRVENSHTVV 144
E L+ + Q + ++ L+ D QN EA ++NS
Sbjct: 61 ERLKRDEQLLNGVLKKYDAKQEVLS---------PEELRDAQNFLEMQEANSLDNSIRGT 111
Query: 145 DSLIDQAYETREQFMSQRVFLQRSSDRILQTLQHIPLINNVISKINTRRKKNAVILASLI 204
+ L+++AY TRE F Q L ++RI IP IN ++ K + RR+++++ILA LI
Sbjct: 112 NELLERAYATREDFDYQNSVLGNVTNRINGAAMSIPFINQILRKTSIRRRRDSIILALLI 171
Query: 205 SICILVLFF 213
S+ +L+ F
Sbjct: 172 SVLMLLFLF 180
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.125 0.319
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,965,091
Number of Sequences: 60738
Number of extensions: 362551
Number of successful extensions: 2413
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2403
Number of HSP's gapped (non-prelim): 7
length of query: 215
length of database: 30,389,216
effective HSP length: 102
effective length of query: 113
effective length of database: 24,193,940
effective search space: 2733915220
effective search space used: 2733915220
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)