ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0229 good R KOG2750 General function prediction only Uncharacterized conserved protein similar to ATP/GTP-binding protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0229 82495 84387 631
(631 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLL035w [R] KOG2750 Uncharacterized conserved protein similar to... 403 e-112
SPCC830.03 [R] KOG2750 Uncharacterized conserved protein similar... 100 9e-21
At5g11010 [R] KOG2750 Uncharacterized conserved protein similar ... 77 8e-14
7302997 [R] KOG2750 Uncharacterized conserved protein similar to... 65 3e-10
CE19989 [R] KOG2750 Uncharacterized conserved protein similar to... 55 2e-07
At3g04680 [A] KOG2749 mRNA cleavage and polyadenylation factor I... 52 3e-06
>YLL035w [R] KOG2750 Uncharacterized conserved protein similar to
ATP/GTP-binding protein
Length = 632
Score = 403 bits (1035), Expect = e-112
Identities = 238/648 (36%), Positives = 372/648 (56%), Gaps = 42/648 (6%)
Query: 3 EDILTYTANTDSD-DSDVEQREI-EKKYKTSSETPVVQLXXXXXXXXXXIRETNGNDFPN 60
+D+ YT ++ S+ DSD I E SS++ V L N ++ +
Sbjct: 7 QDLPQYTKDSGSESDSDSSNNFIVESPSIPSSKSATVVL--------------NSEEYED 52
Query: 61 EE-DESNQLYSPVVDMNFFTLSDECTEVIICLEKNQHLLICGQFNLQIIKGGITYNNVHY 119
+E D+ N L + ++D N DE + + L++ Q L + G F LQ++KGGI YNNVHY
Sbjct: 53 DEGDDLNGLDAELID-NITYEGDEDETMFVGLKEKQKLHLSGVFRLQVVKGGIVYNNVHY 111
Query: 120 NSGYKNWEFWHPACNAISPIVSSFYAGWESKLFL--NREYQHFTTQIESYDCVLKI--SN 175
N+ + FWHP +I I S +AGW+ F+ N ++ + +S+ CVL++ SN
Sbjct: 112 NASREILTFWHPLSQSIPTIDFSHFAGWQDTFFMPRNNRFKIRDEEFKSFPCVLRVFNSN 171
Query: 176 GCNITELSSLLPELRDMWGPLNISLLPGIARKQLTFNIISKISDSVRILDISTEWSTVID 235
+ E L ++ +W P P ++ T++++ + SD ++ L + WST ++
Sbjct: 172 HTGLLEAGHLYRDVNYLWKPKE-PYFP--LNERTTYHLLHE-SDRIQSLSVPGYWSTPLE 227
Query: 236 EMRIFHANSSQDMRVMVIGGKNSGKSTLMRLLVQKFLHSNND--LSYEAIHYLDIDPGQP 293
++ + H N++ D R+MVIGGKNSGKST +RLL++KF D S E + YLD+DPGQP
Sbjct: 228 KLYLSHKNAAYDTRIMVIGGKNSGKSTFLRLLLEKFTQDIRDSTTSQEELVYLDLDPGQP 287
Query: 294 EYSPPESISWNKVDPKSMSLGQHLCQG-RFTTIKQHFIGSSSPQDWPETYSNAVESLLKE 352
EYS P+SIS NK+ +SLGQHLCQG F T+ Q + GSSSPQD P +Y N + L+
Sbjct: 288 EYSLPDSISLNKILSSPISLGQHLCQGSNFQTLLQFYAGSSSPQDEPTSYLNCADKLIDH 347
Query: 353 WQTENFMGTSFLNIPGWIKGFGVKIINHALDHFKPTHIIFL-SYNGLPFSSEIKVPDAFS 411
+ + F GTS LN+PGWIKGFG++I+NH + +KPTH++FL + N E+ +P +FS
Sbjct: 348 LEEQAFFGTSLLNLPGWIKGFGMQILNHIIRKYKPTHLLFLETANSKRHLDELTIPQSFS 407
Query: 412 TTQRSTYKPIILQLHSPAVGKHISHALQRFNAAKIRQFKITAYLHKKYDRNFVINPLVMQ 471
T+ R Y P ++++ + ++ +S RF+A+++R FKI A HK ++ PL+
Sbjct: 408 TSLRDAYAPEVVRVPAHSLNHTLS---SRFHASQLRTFKILALFHKITQFDYDFAPLLKS 464
Query: 472 KPQLTSI--GNRGIVGFEFLQYSNSSLYHDDIKLSLDGTVVALHRADSTPDVLFKGI--F 527
P S G GI G +F L DIK +L+GTV+ ++ + K + F
Sbjct: 465 APLQISYGKGKSGIKGIQF-PMEFQDLNPQDIKSALEGTVIGIYTYSGEDSLEVKSLNTF 523
Query: 528 PVMKNYSKINTEFVGLALIHSIDLEQSLIYMYIPENLFRAL--RETENKWVLVRGKTETP 585
P++++ + + F+ L LIHSID Q ++ +Y+P + L + + +W++VR KTETP
Sbjct: 524 PILQSCTSSSKNFITLGLIHSIDTSQQIMNIYVPPCHTQILDKQPEDAQWIIVRNKTETP 583
Query: 586 ICELYPN--NGIFKSEVDIPYVSLYQKKRHEHVWKVRKNVMRRGHHMK 631
C+ P+ + + IP+ + ++K+ EHVWKVRKNVMRRG MK
Sbjct: 584 FCDFLPSPRTITWDDNIQIPFATFERRKKLEHVWKVRKNVMRRGQFMK 631
>SPCC830.03 [R] KOG2750 Uncharacterized conserved protein similar to
ATP/GTP-binding protein
Length = 736
Score = 100 bits (248), Expect = 9e-21
Identities = 148/639 (23%), Positives = 262/639 (40%), Gaps = 99/639 (15%)
Query: 52 ETNGNDFPNEEDESNQLYS--PVVDMNFFTLSDECTEVIICLEKNQHLLICGQFNLQIIK 109
ET + P+ SN + + N F + D + + L K+Q L + G F + ++
Sbjct: 128 ETVSENVPHSPQSSNDAIPVIKITEENSFRVKDT---LYVGLHKDQKLAMVGTFIFRSVR 184
Query: 110 GGITYNNVHYNSGYKNWEFWHPACNAISPIVSSFYAGWESKLFLNREYQHFTTQIESYDC 169
G Y+S +W + +P+ S+ + EY+ + + S+
Sbjct: 185 GKFQLFGATYSSACMSWFPLNAPLAFATPVFSAIDNALPAMQI--SEYEEDSLNLRSF-A 241
Query: 170 VLKISNGCNITELS--SLLPELRDMW-------GPLNISLLPGIARKQLTF-NIISKISD 219
V S + EL +LP + G I+ + A++ +F N++ S
Sbjct: 242 VPSYSPKEHEYELEPKDILPNFETVIAFRENENGLSKIARVLPFAKRLFSFKNLLQDASS 301
Query: 220 SVRILDISTEWSTVIDEMRIFHANSSQDM--RVMVIGGKNSGKSTLMRLLVQKFLHSNND 277
+I+ E +F + S + R+MV G K SGKS+ R + + L
Sbjct: 302 ISNNFEITPESWCSFSNQLLFSTSKSDYVVPRLMVCGPKGSGKSSFSRYITNRLLQQ--- 358
Query: 278 LSYEAIHYLDIDPGQPEYSPPESISWNKVDPKSMSLGQHLCQGRFTT--IKQHFIGSSSP 335
Y I YLD+DPGQPE P IS ++ S G + F T ++ H +G SP
Sbjct: 359 --YRHIAYLDLDPGQPEVVPSGHISLYYIN--SPLQGPVFARMLFPTYMLRLH-LGDISP 413
Query: 336 QDWPETYSNAVESLLKEWQTENF--------MGTSFLNIPGWIKGFGVKIINHALDHFKP 387
Q P+ Y V L E++ F + +N PGWI+G G ++++ +D +P
Sbjct: 414 QKDPDHYIACVTRLFAEYKDYIFNQEISQKEIIPLIINCPGWIRGGGAELLSSIVDICQP 473
Query: 388 THIIFLSYNGLPFSSEIK---------VPDAFSTTQRSTYKPIILQ-----LHSPAVGKH 433
T ++++S + S K +PD S+ R ++ +L+ L P V K
Sbjct: 474 TEVVYMSREDMKSSHREKKSIYQHKEYMPDFLSS--RDEFQLTLLESTWQYLPDPNVNK- 530
Query: 434 ISHALQRFNAAKIRQFKITAYLH--KKYDRNFVINPLVMQKPQLTSIGNRGIVGFEFLQY 491
+A R + +YL+ R L +P T+ G + +
Sbjct: 531 -------VTSADNRMLGLLSYLYFNCNLQRWDFTTSLTACQPIATAFKGSS-KGIDAVNI 582
Query: 492 SNSSLYHDDIKLSLDGTVVALHRAD-------------STPD---VLFKGIFPVMKNYSK 535
L +D+ +++GT++AL+ D S+P+ ++ P+ N S
Sbjct: 583 IGEPLNVNDVAKTINGTLMALYACDTASLDNSNTQRIVSSPEGIPLIINDGLPLDPNTS- 641
Query: 536 INTEFVGLALIHSIDLEQSLIYMYIPEN--LFRALRETENKWVLVRGKTETPICELYPNN 593
+GL ++ +IDL+++ + P N L + K VL RG+ E P+ + +
Sbjct: 642 ---HCLGLIVLRTIDLKRNEFHFVGPLNLELIKDAYAKSLKIVLERGRLELPVYAMLDHR 698
Query: 594 GIFKSEVDIPYVSLYQKKRHE------HVWKVRKNVMRR 626
SE +PY+ + H+ H +VR+N++RR
Sbjct: 699 LAQYSE--LPYLD----RNHDRVAVGAHRRRVRRNIIRR 731
>At5g11010 [R] KOG2750 Uncharacterized conserved protein similar to
ATP/GTP-binding protein
Length = 380
Score = 77.0 bits (188), Expect = 8e-14
Identities = 87/386 (22%), Positives = 160/386 (40%), Gaps = 56/386 (14%)
Query: 226 ISTEWSTVIDEMRIFHANSSQDMRVMVIGGKNSGKSTLMRLLVQKFLHSNNDLSYEAIHY 285
I EWS + +S Q + +V G KNSGKST R LV+ L Y+ + Y
Sbjct: 11 IPEEWSNAASSVS---CSSLQPVIALVCGPKNSGKSTFSRNLVEVLLQR-----YKRVAY 62
Query: 286 LDIDPGQPEYSPPESISWNKVDPKSMSLGQ------HLCQGRFTTI------KQHFIGSS 333
LD D GQPE++ P +S VD KS+ + + + +T + F G
Sbjct: 63 LDTDVGQPEFTAPGFLSLTIVD-KSILVTYVSGYIFYFVESDWTVPCVKTPERCFFYGDV 121
Query: 334 SPQDWPETYSNAVESLLKEWQTENFMGTS--------FLNIPGWIKGFGVKIINHALDHF 385
S + P+ Y V +L +Q +F +S +N PGW+KG G +++ L +
Sbjct: 122 SSKRDPKAYLRYVYTLFDYYQL-HFCKSSENKTELPLVINTPGWVKGIGYELLVDVLRYV 180
Query: 386 KPTHIIFLSYNGLPFSSEIKVPDAFSTTQRSTYKPIILQLHSPAVGKHISHALQRFNAAK 445
P+H++ ++ + ++ + F ++++ S ++ L +A
Sbjct: 181 SPSHVVKINISA--YNKNLPA-GLFWLDGNDDETAHLIEIQSAYQDRYNQSILIHKDARL 237
Query: 446 IRQFKITAYLHKKYDRNFVINPLVMQKPQLTSIGNRGIVGFEFLQYSNSSLYHDDIKLSL 505
+R +I AY + + V + + I + + + + + ++ SL
Sbjct: 238 MRDMRIIAYFRQCFKGKEVNTIKELTHELASHIPYEVPISSLTINHLHCQIPSSEVYYSL 297
Query: 506 DGTVVALHRADSTPDVLFKGIFPVMKNYSKINTEFVGLALIHSIDLEQSLIYMY--IPEN 563
+ ++V L + + L VGL ++ ID E+ ++Y+ +PEN
Sbjct: 298 NASIVGLGISTEVFEDL---------------PSCVGLGIVRGIDTERGILYVITPVPEN 342
Query: 564 LFRALRETENKWVLVRGKTETPICEL 589
L E +L++G + P C L
Sbjct: 343 L------VEKVDLLLQGYIQLPTCLL 362
>7302997 [R] KOG2750 Uncharacterized conserved protein similar to
ATP/GTP-binding protein
Length = 987
Score = 65.1 bits (157), Expect = 3e-10
Identities = 61/235 (25%), Positives = 110/235 (45%), Gaps = 35/235 (14%)
Query: 218 SDSVRILDISTEWSTVIDEMRIFHANSSQDMRVMVIGGKNSGKSTLMRLLVQKFLHSNND 277
SD R L + W+ + HA S R++V GGK GKS+L+R L+ + L
Sbjct: 612 SDQSRTLQVPQVWNKLQ-----MHATS----RIIVAGGKGVGKSSLLRYLINRNLG---- 658
Query: 278 LSYEAIHYLDIDPGQPEYSPPESISWNKVDPKSMSLGQHLCQGRFTTIKQH--FIGSSSP 335
+ ++ +D+D GQPE P++IS +D + G + +H +G ++
Sbjct: 659 -QFPSMLLIDLDIGQPEIFVPQTISCTVIDEPLLGPG-----FLYNRQPEHAIVVGHTNI 712
Query: 336 QDWPETYSNAVESLLKEWQTENFMGTSFLNIP------GWIKGFGVKIINHALDHFKPTH 389
E Y+ AV L++ Q + + NIP G+ KGFG++++ +D +PT
Sbjct: 713 VLCAEQYARAVIQLVQNIQND----AKYSNIPWLINTMGYNKGFGIELMALLVDRIRPTD 768
Query: 390 IIFLSYNGLPFSSEIKVPDAFSTTQRSTYKPIILQLHSPAVGKHISHALQRFNAA 444
++ ++ + +P ++ V D S +Q KPII + + + L + +A
Sbjct: 769 LVQIA-SPIPINNFDSVLDRNSLSQ---IKPIIYSAEEFKINEIPKYTLHKLISA 819
>CE19989 [R] KOG2750 Uncharacterized conserved protein similar to
ATP/GTP-binding protein
Length = 549
Score = 55.5 bits (132), Expect = 2e-07
Identities = 81/352 (23%), Positives = 142/352 (40%), Gaps = 37/352 (10%)
Query: 243 NSSQDMRVMVIGGKNSGKSTLMRLLVQKFLHSNNDLSYEAIHYLDIDPGQPEYSPPESIS 302
N Q ++ IG K +GKS LMR LV + L + YE ++ LD D GQ E++P +S
Sbjct: 179 NDGQRTTILPIGHKGAGKSNLMRSLVNRCLSN----GYEHVYVLDCDIGQSEFTPCGCLS 234
Query: 303 WNKVDPKSMSLGQHLCQGRFTTIKQHFIGSSSPQDWPETYSNAVESLLKEWQTENFMGT- 361
KV S LG+ R + F G + +D Y + E L +++ + G+
Sbjct: 235 LTKV--TSPILGKPHGHQRASFENSFFYGDITVRD-INLYMDIFERLFNKFKVISEPGSV 291
Query: 362 SFLNIPGWIKGFGVKIINHALDHFKPTHIIFLSYNGLPFSSEIKVPDAFSTTQRSTYKPI 421
+N GW+ G +I++ P + + F + + F I
Sbjct: 292 CIINSMGWVTDEGAEILDGITRVTDPELFVEI------FRDQTEARYQFLNRAEHNIVEI 345
Query: 422 ILQLHSPAVGKHISHALQRFNAAKIRQFKITAYLHKKYDRNFVINPLVMQKPQLTSIGNR 481
+G +R AA IR+ I Y + R + + + +L
Sbjct: 346 FANNSIGVIG---LPPQKRLPAALIRELTIVGYFSSRLPRPSLASFPKVAPYKLRFENVT 402
Query: 482 GIVGFEFL---QYSNSSLYHDDIKLSLDGTVV---ALHRADSTPDV-LFKGIFPVMKNYS 534
V + L + SS+ + L ++ T + L+ D P + + G P +
Sbjct: 403 ICVPVDLLVEDSHVFSSINTQIMALCINNTDLKPRKLYGKDDLPSICVIDGNSPAL---- 458
Query: 535 KINTEFVGLALIHSIDLEQSLIYMYIPENLFRALRETENKWVLVRG-KTETP 585
+ +G +I + +E+ +I++ P +L + E VLVRG + +TP
Sbjct: 459 ----QCIGFGIIRGVSVEERIIFVVTPVDLLK----LEEPPVLVRGMRIQTP 502
>At3g04680 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 444
Score = 52.0 bits (123), Expect = 3e-06
Identities = 57/228 (25%), Positives = 98/228 (42%), Gaps = 54/228 (23%)
Query: 244 SSQDMRVMVIGGKNSGKSTLMRLLVQKFLHSNNDLSYEAIH-----YLDIDPGQPEYSPP 298
SSQ RV+V+G +SGKSTL ++L LS+ A ++D+D GQ + P
Sbjct: 127 SSQGPRVIVVGPTDSGKSTLTKML----------LSWAAKQGWRPTFVDLDVGQGSITIP 176
Query: 299 ESISWNKVD---------PKSMSLGQHLCQGRFTTIKQHFIGSSSPQDWPETYSNAVESL 349
SI+ ++ P M+L ++ G +SP E Y V+ L
Sbjct: 177 GSIAAAPIEMPLDPVEGFPLDMAL-------------VYYYGHASPNMNVELYKALVKEL 223
Query: 350 LKEWQTENFMGTS-------FLNIPGWIKGFGVKIINHALDHFKPTHIIFLSYNGLPFSS 402
+ + + F+G +N GWI+G G +++ HA+D F + ++ L
Sbjct: 224 AQVLEKQ-FVGNPESRAAGMVINTMGWIEGIGYELLLHAIDTFNASVVLVL--------G 274
Query: 403 EIKVPDAFSTTQRSTYKPIILQLH-SPAVGKHISHALQRFNAAKIRQF 449
+ K+ RS +++LH S V + +R KI+++
Sbjct: 275 QEKLFSRLKDVLRSKSNVDVVKLHKSGGVVARVKEVRKRSRNFKIQEY 322
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,626,096
Number of Sequences: 60738
Number of extensions: 1755367
Number of successful extensions: 4748
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 4728
Number of HSP's gapped (non-prelim): 7
length of query: 631
length of database: 30,389,216
effective HSP length: 113
effective length of query: 518
effective length of database: 23,525,822
effective search space: 12186375796
effective search space used: 12186375796
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)