ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0244 good I KOG2208 Lipid transport and metabolism Vigilin
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0244 90132 87994 -713
(713 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLL032c [I] KOG2208 Vigilin 278 3e-74
ECU06g1470 [I] KOG2208 Vigilin 83 2e-15
SPBC28E12.02 [I] KOG2208 Vigilin 81 5e-15
7302610 [I] KOG2208 Vigilin 64 1e-09
Hs4885409 [I] KOG2208 Vigilin 53 2e-06
>YLL032c [I] KOG2208 Vigilin
Length = 825
Score = 278 bits (710), Expect = 3e-74
Identities = 178/542 (32%), Positives = 286/542 (51%), Gaps = 23/542 (4%)
Query: 159 QHKYVDVIDFKAVSMMPLLAGQNGGNWKHLSECFHTEVIDSQLGILGHDYPS--KLFLQS 216
QH ++ ++ + S++PL G + N+KH+S F T + L L D + ++F
Sbjct: 182 QHYIIEPMELNSYSVLPLYMGVDMENFKHISRAFKTSIYAPSLITLSRDLKANPQIFFSG 241
Query: 217 EQKCDALLAKQCLEKKLNDILDGSLHATAIPNVSPLKIAYLKTVFPSETIMKLMQKYPSY 276
+LLA++ L + ++ + S + N++P K+ +++ + + + +L+ KY S
Sbjct: 242 AVHSLSLLARKTLRESIS-VNSKSFFYRRLTNITPGKLLFIRKYY-QQKVNQLILKYQSL 299
Query: 277 INYTTGSVQLQSNSAALLDIVRKRFVRNILAVISEVRFWFTESNDATELINRTSTFKRIA 336
I T ++ QS S LL++V K F +L I EV+ E+ + + S F
Sbjct: 300 IRVTNEYIEFQSISTNLLEMVIKNFTIQVLHEIVEVQISLNENCAMSPELIIDSFFGHTG 359
Query: 337 SKNNVIFSTDKFKNFFNFVGCHASIQQT-------YL-DILAEDFQCV----QNVRFTLE 384
++ VI + N VG +S + YL D + Q + + ++ E
Sbjct: 360 NQIVVITPKEDSFNQLIVVGNQSSTDEASDTSILHYLSDFIMGSNQVINPNLRQIKAIFE 419
Query: 385 LDTIYKDFISGKKNGKLMKIMDRKKCAISLENDSSGHMLVI-LQGEDWQQIQDSFSLLLQ 443
+ ++DFISGKKNGKL +IM+ C I LE + L + L + + ++SF ++
Sbjct: 420 IHPDFEDFISGKKNGKLTRIMELSACLIQLEMEEEDDNLYLNLVSDSFPDFKESFKNVIN 479
Query: 444 ELPCEKSFFIPEAYHRPVIGTGGSVIQTIMRKYNVFIQFSNSFQLPQGDWTFTRYDNVII 503
E P E+SFFIPE HRP+IGTGGS+IQ MRK+NVFIQFSNSF LPQ + RYDNVII
Sbjct: 480 EFPAEESFFIPEVCHRPIIGTGGSLIQATMRKHNVFIQFSNSFNLPQNKISMIRYDNVII 539
Query: 504 RCPSKNTINIEAAKEQLLSLSEQYSQVQKTVYVKMSPSQYLYLIHMNSDLSFISEIEKAH 563
RCP KN NI AK L + ++Y +Q ++ S QY +++H+N + I +IEK
Sbjct: 540 RCPRKNKANICLAKNDLKQIVQEYDSLQSKTLIRFSSGQYRHILHVNGQKNIIGQIEKNE 599
Query: 564 SCYISFPQSLPVEG-YHINVKGNDTGPERAANDII-SRFGREMEIHIK---NPASENEFS 618
+ YI P P++G ++++GND RAAN+++ S FG E E I +P E EF
Sbjct: 600 NVYIMIPLKEPLDGTSQLSIQGNDENASRAANELVNSAFGYEYEFKIDQEIDPNKEYEFY 659
Query: 619 KGLLLP-LSYSNVQFTQNRNFIRLTFSPLMKPSQLEEIIFLIDSFLKLSNNKIISKREVA 677
+++P L N+ T ++ I TF + L + I L+ ++L+ KII K+ +
Sbjct: 660 NLIVVPFLQIMNIIVTFEKDLITFTFEKDTNENTLTKAIELLSNYLETQKTKIIFKKIIK 719
Query: 678 HY 679
+
Sbjct: 720 KF 721
>ECU06g1470 [I] KOG2208 Vigilin
Length = 599
Score = 82.8 bits (203), Expect = 2e-15
Identities = 53/171 (30%), Positives = 90/171 (51%), Gaps = 9/171 (5%)
Query: 355 VGCHASI--QQTYLDILAEDFQCVQNVRFTLELDTIYKDFISGKKNGKLMKIMDRKKCAI 412
VGC I ++T L I+ E+ V+ +E +T+ +F+ GKKNGK+ +IM C I
Sbjct: 302 VGCFMIIGEKETLLKIVEENGGYVE-AEMDIEAETV--EFLCGKKNGKITRIMRDVCCGI 358
Query: 413 SLENDSSGHMLVILQGEDWQQIQDSFSLLLQELPCEKSFFIPEAYHRPVIGTGGSVIQTI 472
+ + ++ Q + ++ E P E +F+I E +H+ +IG GG IQ I
Sbjct: 359 VIHKKQGSPRIHMVINGCSGAFQKAMQMIEDEFPEELTFYIDEKHHKRIIGYGGKNIQKI 418
Query: 473 MRKYNVFIQFSNSFQLPQGDWTFTRYDNVIIRCPSKNTINIEAAKEQLLSL 523
M+K+ V+I+F N + + + DNV+I+ P KN N+ + K ++ L
Sbjct: 419 MKKHGVYIKFMNERERRRSAYR----DNVVIKTPRKNVGNLISMKNDVMEL 465
>SPBC28E12.02 [I] KOG2208 Vigilin
Length = 663
Score = 81.3 bits (199), Expect = 5e-15
Identities = 61/210 (29%), Positives = 100/210 (47%), Gaps = 36/210 (17%)
Query: 324 ELINRTSTFKRIASKNNVIFSTDKFKNFFNFVGCHASIQQTYLDILAEDFQCVQNVRFTL 383
E++ +T+ + +N V + DK F G Q + IL L
Sbjct: 469 EMLLKTNGIEYFGQENTVPIAMDKASKIFYKFG-----QSQWHQIL-------------L 510
Query: 384 ELDTIYKDFISGKKNGKLMKIMDRKKCAISLEN-------DSSGHMLVILQGEDWQQIQD 436
E T +DFISGKKNGKL K+ +++C +L+N S+ V + ++ +++
Sbjct: 511 EAPTKDQDFISGKKNGKLDKV--KQQCRFNLKNGDILFCPQSTSIFTVDIYSDELERVIK 568
Query: 437 SFSLLLQELPCEKSFFIPEAYHRPVIGTGGSVIQTIMRKYNVFIQFSNSFQLPQGDWTFT 496
+ +L E P E F++PE H+ +IG G IQ + + YN +I+FS + F
Sbjct: 569 GMNTMLLEFPAEMHFYVPEEIHKKLIGFRGEQIQRVTKLYNSYIEFSTT--------PFD 620
Query: 497 RY-DNVIIRCPSKNTINIEAAKEQLLSLSE 525
Y NV+IR PSK + N+ A + + +E
Sbjct: 621 CYGHNVLIRTPSKFSENLWAVRSLFIKTAE 650
>7302610 [I] KOG2208 Vigilin
Length = 1268
Score = 63.5 bits (153), Expect = 1e-09
Identities = 57/218 (26%), Positives = 95/218 (43%), Gaps = 29/218 (13%)
Query: 389 YKDFISGKKNGKLMKIMDRKKCAISLENDSSGHMLVILQG---------EDWQQIQDSFS 439
+ F+ GK + KI D + I L + + ++++ G E Q+IQ+ S
Sbjct: 574 FHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELS 633
Query: 440 LLLQELPCEKSFFIPEAYHRPVIGTGGSVIQTIMRKY-NVFIQFSNSFQLPQGDWTFTRY 498
++ E IP Y+ +IGTGG +I +IM + V I+F NS ++
Sbjct: 634 DIVTE-----EVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFPNS---------DSKS 679
Query: 499 DNVIIRCPSKNTINIEAAKEQLLSLSEQYSQVQKTVYVKMSPSQYLYLIHMNSDLSFISE 558
D V IR P ++E AK QLL L+ + T V+ + +LI N + I +
Sbjct: 680 DKVTIRGPKD---DVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNG--ASIRK 734
Query: 559 IEKAHSCYISFPQSLPVEGYHINVKGNDTGPERAANDI 596
I A I FP + + I + G + ++A +
Sbjct: 735 IRDATGARIIFPSNEDTDKEVITIIGKEESVKKAREQL 772
>Hs4885409 [I] KOG2208 Vigilin
Length = 1268
Score = 52.8 bits (125), Expect = 2e-06
Identities = 58/231 (25%), Positives = 101/231 (43%), Gaps = 30/231 (12%)
Query: 388 IYKDF---ISGKKNGKLMKIMDRKKCAISLENDSSGHMLVILQGE--DWQQIQDSFSLLL 442
I+K F I GK + KI + I L ++S +I+ G+ + + + +
Sbjct: 588 IFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQ 647
Query: 443 QELP--CEKSFFIPEAYHRPVIGTGGSVIQTIMRKYN-VFIQFSNSFQLPQGDWTFTRYD 499
++L E IP H +IGT G +I++IM + V I F +G + D
Sbjct: 648 KDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFP-----VEGSGS----D 698
Query: 500 NVIIRCPSKNTINIEAAKEQLLSLSEQYSQVQKTVYVKMSPSQYLYLIHMNSDLSFISEI 559
V+IR PS + +E AK+QLL L+E+ TV ++ P + +LI I ++
Sbjct: 699 TVVIRGPSSD---VEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGG--GKIRKV 753
Query: 560 EKAHSCYISFPQSLPVEGYHINVKGNDTGPERAANDIISRFGREMEIHIKN 610
+ + FP + + I + G + D + +E+E I+N
Sbjct: 754 RDSTGARVIFPAAEDKDQDLITIIGKE--------DAVREAQKELEALIQN 796
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,326,635
Number of Sequences: 60738
Number of extensions: 1833267
Number of successful extensions: 4094
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4069
Number of HSP's gapped (non-prelim): 15
length of query: 713
length of database: 30,389,216
effective HSP length: 114
effective length of query: 599
effective length of database: 23,465,084
effective search space: 14055585316
effective search space used: 14055585316
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)