ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0357 good I KOG2848 Lipid transport and metabolism 1-acyl-sn-glycerol-3-phosphate acyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0357 128491 127547 -315
(315 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL052c [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase 324 1e-88
SPAC1851.02 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltrans... 197 1e-50
Hs6041665 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransfe... 124 2e-28
CE11552 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase 121 1e-27
7292807 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase 120 3e-27
Hs5453718 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransfe... 117 2e-26
CE06378 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase 110 2e-24
7297421 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase 102 9e-22
At4g30580 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransfe... 90 4e-18
CE28186 [I] KOG1505 Lysophosphatidic acid acyltransferase LPAAT ... 53 5e-07
>YDL052c [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 303
Score = 324 bits (830), Expect = 1e-88
Identities = 158/299 (52%), Positives = 212/299 (70%)
Query: 1 MGFFSKIVFWGKSVIAVTLLSSCALYGVLASIVFSLIGKKHLAQWSTARCFYGVMGFIMG 60
M + +++ +SV+ V L+ C YGV+ASI+ +LIGK+HLAQW TARCFY VM ++G
Sbjct: 1 MSVIGRFLYYLRSVLVVLALAGCGFYGVIASILCTLIGKQHLAQWITARCFYHVMKLMLG 60
Query: 61 IKINLINGERLNDLPAIFISNHQSELDILMLGRTFPPGCTVTAKKQIKWVPLLGWFMSLS 120
+ + ++ E L P I I+NHQS LDI MLGR FPPGCTVTAKK +K+VP LGWFM+LS
Sbjct: 61 LDVKVVGEENLAKKPYIMIANHQSTLDIFMLGRIFPPGCTVTAKKSLKYVPFLGWFMALS 120
Query: 121 GTLFLDRSSREKAIKVLNDSLANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFHLAQQ 180
GT FLDRS R++AI LN L N+KK+KRALW+FPEGTRSY+++L + FKKGAFHLAQQ
Sbjct: 121 GTYFLDRSKRQEAIDTLNKGLENVKKNKRALWVFPEGTRSYTSELTMLPFKKGAFHLAQQ 180
Query: 181 GKIQIIPVVVSNTSSIYHPRSGVFNRGTITVKVLEPISTENLKKEDVGDLCNRVHKLMND 240
GKI I+PVVVSNTS++ P+ GVFNRG + V++L+PISTENL K+ +G+ +V M D
Sbjct: 181 GKIPIVPVVVSNTSTLVSPKYGVFNRGCMIVRILKPISTENLTKDKIGEFAEKVRDQMVD 240
Query: 241 ELKNIGYSEAIVDTVLPADVIAYNSARRFEDQHRLTADDYEEEIVDLDRSSIHSNGTVV 299
LK IGYS AI DT LP I Y + + + ++ ++ + + + H+ G+ V
Sbjct: 241 TLKEIGYSPAINDTTLPPQAIEYAALQHDKKVNKKIKNEPVPSVSISNDVNTHNEGSSV 299
>SPAC1851.02 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 279
Score = 197 bits (502), Expect = 1e-50
Identities = 100/229 (43%), Positives = 143/229 (61%), Gaps = 1/229 (0%)
Query: 18 TLLSSCALYGVLASIVFSLIGKKHLAQWSTARCFYGVMGFIMGIKINLINGERLND-LPA 76
T L+ + YGV+AS+V L G + Q+ TA+ +YG+ I+ + + N E L A
Sbjct: 42 TFLAISSAYGVIASVVARLCGYPVMGQYLTAKAYYGLASTILDFRFKIENEEILRKHKSA 101
Query: 77 IFISNHQSELDILMLGRTFPPGCTVTAKKQIKWVPLLGWFMSLSGTLFLDRSSREKAIKV 136
+ + NHQSELDIL +GRTF P +V AKK +++VP+LGWFM LS +F+DRS R AI++
Sbjct: 102 VLVVNHQSELDILAIGRTFGPNYSVIAKKSLRYVPILGWFMILSDVVFIDRSRRSDAIQL 161
Query: 137 LNDSLANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFHLAQQGKIQIIPVVVSNTSSI 196
+ ++K+ ++W+F EGTRSYS K L KKGAFHLA Q ++ IIP+ + +
Sbjct: 162 FAKAARRMRKENISIWVFAEGTRSYSLKPCLLPLKKGAFHLAVQAQVPIIPIAIQTYGHL 221
Query: 197 YHPRSGVFNRGTITVKVLEPISTENLKKEDVGDLCNRVHKLMNDELKNI 245
+HP + VFN+G +KVL+PI TE EDV DL + MN+ L I
Sbjct: 222 FHPPTKVFNKGEALIKVLDPIPTEGKTAEDVNDLLHETETAMNNALVEI 270
>Hs6041665 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 278
Score = 124 bits (310), Expect = 2e-28
Identities = 75/225 (33%), Positives = 119/225 (52%), Gaps = 4/225 (1%)
Query: 23 CALYGVLASIVFSLI-GKKHLAQWSTARCFYGVMGFIMGIKINLINGERLNDL-PAIFIS 80
C +AS+V L G + + S F + G++ + + RL + P + +S
Sbjct: 37 CFTVSAVASLVCLLRHGGRTVENMSIIGWFVRSFKYFYGLRFEVRDPRRLQEARPCVIVS 96
Query: 81 NHQSELDILMLGRTFPPGCTVTAKKQIKWVPLLGWFMSLSGTLFLDRSSREKAIKVLNDS 140
NHQS LD++ L P C AK+++ ++ +G M L G F++R A+ V+ D
Sbjct: 97 NHQSILDMMGLMEVLPERCVQIAKRELLFLGPVGLIMYLGGVFFINRQRSSTAMTVMADL 156
Query: 141 LANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFHLAQQGKIQIIPVVVSNTSSIYHPR 200
+ ++ +WI+PEGTR+ + +L FKKGAF+LA Q ++ I+PVV S+ SS Y+ +
Sbjct: 157 GERMVRENLKVWIYPEGTRNDNG--DLLPFKKGAFYLAVQAQVPIVPVVYSSFSSFYNTK 214
Query: 201 SGVFNRGTITVKVLEPISTENLKKEDVGDLCNRVHKLMNDELKNI 245
F GT+TV+VLE I T L DV L + H+ M +I
Sbjct: 215 KKFFTSGTVTVQVLEAIPTSGLTAADVPALVDTCHRAMRTTFLHI 259
>CE11552 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 262
Score = 121 bits (304), Expect = 1e-27
Identities = 79/239 (33%), Positives = 124/239 (51%), Gaps = 15/239 (6%)
Query: 1 MGFFSKIVFWGKSVIAVTLLSSCALYGVLASIVFSLIGKKHLAQWSTARCFYGVMGFIMG 60
+GF+ + V++G +I L G LASI F H + + M + MG
Sbjct: 22 IGFYIRAVYFGMCLIIGGFL------GGLASIPFGKSPNNHFRMFK----IFQAMTWPMG 71
Query: 61 IKINLINGERLNDL-PAIFISNHQSELDILMLGRTFPPGCTVTAKKQIKWVPLLGWFMSL 119
++ L N E L+D P I I+NHQS LD+L + +P C V K +K++P L
Sbjct: 72 VRFELRNSEILHDKKPYIIIANHQSALDVLGMSFAWPVDCVVMLKSSLKYLPGFNLCAYL 131
Query: 120 SGTLFLDRSSREKAIKVLNDSLANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFHLAQ 179
+++++R S+EKA+K ++ +L + KR +WI+PEGTR + + EL FKKGAF LA+
Sbjct: 132 CDSVYINRFSKEKALKTVDTTLHEIVTKKRKVWIYPEGTR--NAEPELLPFKKGAFILAK 189
Query: 180 QGKIQIIPVVVSNTSSIYHPRSGVFNRGTITVKVLEPISTENLKKEDVGDLCNRVHKLM 238
Q KI I+P V S+ Y G + +L + ++ K + + DL K+M
Sbjct: 190 QAKIPIVPCVFSSHKFFYSHAEKRLTSGNCIIDILPEV--DSSKFDSIDDLSAHCRKIM 246
>7292807 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 343
Score = 120 bits (300), Expect = 3e-27
Identities = 73/202 (36%), Positives = 110/202 (54%), Gaps = 7/202 (3%)
Query: 45 WSTARCFYGVMGFIMGIKINLINGERL-NDLPAIFISNHQSELDILMLGRTFPP--GCTV 101
W++ C + ++G++ L E L D I ++NHQS LD+L + + CTV
Sbjct: 65 WASTWCHR--VSTLIGLRWELRGKEHLAKDQACIIVANHQSSLDVLGMFNIWHVMNKCTV 122
Query: 102 TAKKQIKWVPLLGWFMSLSGTLFLDRSSREKAIKVLNDSLANLKKDKRALWIFPEGTRSY 161
AK+++ + G L+G +F+DR EKA + LND +KK + LW+FPEGTR
Sbjct: 123 VAKRELFYAWPFGLAAWLAGLIFIDRVRGEKARETLNDVNRRIKKQRIKLWVFPEGTRRN 182
Query: 162 STKLELSAFKKGAFHLAQQGKIQIIPVVVSNTSSIYHPRSGVFNRGTITVKVLEPISTEN 221
+ L FKKGAFH+A +I I+PVV S+ + + + + N G I + L P+STE
Sbjct: 183 TGALH--PFKKGAFHMAIDQQIPILPVVFSSYCTFLNDKKKILNSGRIVITTLPPVSTEG 240
Query: 222 LKKEDVGDLCNRVHKLMNDELK 243
L K+D+ L RV M + K
Sbjct: 241 LTKDDIDVLMERVRSQMIETFK 262
>Hs5453718 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 283
Score = 117 bits (294), Expect = 2e-26
Identities = 71/239 (29%), Positives = 118/239 (48%), Gaps = 17/239 (7%)
Query: 3 FFSKIVFWGKSVI--AVTLLSSCALYGVLASIVFSLIGKKHLAQWSTARCFYGVMGFIMG 60
+F K+ F+ ++ AV + CA+ G +++ R + ++ G
Sbjct: 34 YFFKMAFYNGWILFLAVLAIPVCAVRG------------RNVENMKILRLMLLHIKYLYG 81
Query: 61 IKINLINGERLN-DLPAIFISNHQSELDILMLGRTFPPGCTVTAKKQIKWVPLLGWFMSL 119
I++ + P + +SNHQS LD+L + P C AK+++ W G L
Sbjct: 82 IRVEVRGAHHFPPSQPYVVVSNHQSSLDLLGMMEVLPGRCVPIAKRELLWAGSAGLACWL 141
Query: 120 SGTLFLDRSSREKAIKVLNDSLANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFHLAQ 179
+G +F+DR AI V+++ L +W+FPEGTR+++ + FK+GAFHLA
Sbjct: 142 AGVIFIDRKRTGDAISVMSEVAQTLLTQDVRVWVFPEGTRNHNG--SMLPFKRGAFHLAV 199
Query: 180 QGKIQIIPVVVSNTSSIYHPRSGVFNRGTITVKVLEPISTENLKKEDVGDLCNRVHKLM 238
Q ++ I+P+V+S+ Y + F G V+VL P+ TE L +DV L +RV M
Sbjct: 200 QAQVPIVPIVMSSYQDFYCKKERRFTSGQCQVRVLPPVPTEGLTPDDVPALADRVRHSM 258
>CE06378 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 282
Score = 110 bits (276), Expect = 2e-24
Identities = 62/172 (36%), Positives = 95/172 (55%), Gaps = 3/172 (1%)
Query: 75 PAIFISNHQSELDILMLGRTFPPGCTVTAKKQIKWVPLLGWFMSLSGTLFLDRSSREKAI 134
PA+ I NHQS LDIL + +P C V K+ + +VP S T+F+DR +RE+A+
Sbjct: 91 PAVVICNHQSSLDILSMASIWPKNCVVMMKRILAYVPFFNLGAYFSNTIFIDRYNRERAM 150
Query: 135 KVLNDSLANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFHLAQQGKIQIIPVVVSNTS 194
++ + +K LW+FPEGTR + + FKKGAF++A + +I IIPVV S+
Sbjct: 151 ASVDYCASEMKNRNLKLWVFPEGTR--NREGGFIPFKKGAFNIAVRAQIPIIPVVFSDYR 208
Query: 195 SIYHPRSGVF-NRGTITVKVLEPISTENLKKEDVGDLCNRVHKLMNDELKNI 245
Y F N G + ++VL+ I T+ L +DV +L + +M K +
Sbjct: 209 DFYSKPGRYFKNDGEVVIRVLDAIPTKGLTLDDVSELSDMCRDVMLAAYKEV 260
>7297421 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 259
Score = 102 bits (253), Expect = 9e-22
Identities = 68/199 (34%), Positives = 107/199 (53%), Gaps = 6/199 (3%)
Query: 46 STARCFYGVMGFIMGIKINLINGERLN-DLPAIFISNHQSELDILMLGRTFPP--GCTVT 102
+TAR + ++GI + + E + D A+ I NHQS +D+ +L +P TV
Sbjct: 47 ATARLSKCALCRLVGITMEVRGLENVRKDHGAVVIMNHQSAVDLCVLAYLWPVIGRATVV 106
Query: 103 AKKQIKWVPLLGWFMSLSGTLFLDRSSREKAIKVLNDSLANLKKDKRALWIFPEGTRSYS 162
+KK++ ++P G L GTL++DRS + +I L +++ L +FPEGTR +
Sbjct: 107 SKKEVLYIPFFGIGAWLWGTLYIDRSRKTDSINSLQKEAKAIQERNCKLLLFPEGTR--N 164
Query: 163 TKLELSAFKKGAFHLAQQGKIQIIPVVVSNTSSIYHPRSGVFNRGTITVKVLEPISTENL 222
+K L FKKG+FH+A QGK + PVV+S S + + F G + +L +STE
Sbjct: 165 SKDSLLPFKKGSFHIALQGKSPVQPVVISKYSFMDDEKK-TFRPGHALIHILPEVSTEKY 223
Query: 223 KKEDVGDLCNRVHKLMNDE 241
K+EDV L + +M E
Sbjct: 224 KREDVQLLIDECQSIMQTE 242
>At4g30580 [I] KOG2848 1-acyl-sn-glycerol-3-phosphate acyltransferase
Length = 212
Score = 90.1 bits (222), Expect = 4e-18
Identities = 65/187 (34%), Positives = 100/187 (52%), Gaps = 18/187 (9%)
Query: 62 KINLINGERL--NDLPAIFISNHQSELDI---LMLGRTFPPGCTVTAKKQIKWVPLLGWF 116
KIN+ E L +D PA+++SNHQS LDI L LG++F +K I +P++GW
Sbjct: 36 KINIEGLENLPSSDTPAVYVSNHQSFLDIYTLLSLGKSF----KFISKTGIFVIPIIGWA 91
Query: 117 MSLSGTLFLDRSSREKAIKVLNDSLANLKKDKRALWIFPEGTRSYSTKLELSAFKKGAFH 176
MS+ G + L R + L + LKK +++ FPEGTRS + L +FKKGAF
Sbjct: 92 MSMMGVVPLKRMDPRSQVDCLKRCMELLKKG-ASVFFFPEGTRSKDGR--LGSFKKGAFT 148
Query: 177 LAQQGKIQIIPVVVSNTSSIYHPRS-GVFNRGTITVKVLEPISTENLKKEDVGDLCNRVH 235
+A + + ++P+ + T I S G+ N G + V + +PI + K DV LCN
Sbjct: 149 VAAKTGVAVVPITLMGTGKIMPTGSEGILNHGNVRVIIHKPI---HGSKADV--LCNEAR 203
Query: 236 KLMNDEL 242
+ + +
Sbjct: 204 SKIAESM 210
>CE28186 [I] KOG1505 Lysophosphatidic acid acyltransferase LPAAT and related
acyltransferases
Length = 399
Score = 53.1 bits (126), Expect = 5e-07
Identities = 49/142 (34%), Positives = 67/142 (46%), Gaps = 20/142 (14%)
Query: 48 ARCFYGVMGFIMGIKINLINGERLNDLPAIFISNHQSELDILM----LGRTFPPGCT--- 100
A C Y + G + IK + IN E PA+ I NH++ LD L L + P CT
Sbjct: 72 ALCNY-IFGANIRIKGDFINHEE----PALIIMNHRTRLDWLFFWNALYKMDPWLCTTEK 126
Query: 101 VTAKKQIKWVPLLGWFMSLSGTLFLDRSSREKAIKVLNDSLANLKKD---KRALWIFPEG 157
++ K +K+VP GW M + +FLDRS K+ D++ N + K L +FPEG
Sbjct: 127 ISLKGMLKYVPGAGWAMQAASYIFLDRSFDTDKTKL--DNILNYYAETEYKYQLLLFPEG 184
Query: 158 TRSYSTKLELSAF---KKGAFH 176
T E S KKG H
Sbjct: 185 TDKCPKATERSRIHSEKKGLVH 206
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,309,742
Number of Sequences: 60738
Number of extensions: 740947
Number of successful extensions: 2048
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2028
Number of HSP's gapped (non-prelim): 10
length of query: 315
length of database: 30,389,216
effective HSP length: 106
effective length of query: 209
effective length of database: 23,950,988
effective search space: 5005756492
effective search space used: 5005756492
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)