ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0487 good L KOG4062 Replication, recombination and repair 6-O-methylguanine-DNA methyltransferase MGMT/MGT1, involved in DNA repair
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV0487  169765  170331 189  
         (189 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL200c [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT...   115   5e-26
CE22693 [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT...    91   1e-18
7298893 [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT...    83   2e-16
Hs4505177 [L] KOG4062 6-O-methylguanine-DNA methyltransferase MG...    79   4e-15

>YDL200c [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT/MGT1
           involved in DNA repair
          Length = 206

 Score =  115 bits (287), Expect = 5e-26
 Identities = 64/187 (34%), Positives = 100/187 (53%), Gaps = 8/187 (4%)

Query: 1   MSHLLVYDFMEHDLANVLIVVKPSTKSLVFVSLSGTKPKLLGDAKKFVSKVNSLSKNKGS 60
           M  LL Y F+E ++    +V +  T++LVF SL   K  LLG  + F+ K +        
Sbjct: 19  MKELLYYTFIETEVTGAFLVFREKTQNLVFASLGNDKLFLLGKVEGFLKK-HEKQDTMYD 77

Query: 61  IYTLKNKSILKSDDVAWLEQLSFDFLQILAGGIDSQTNIKCEYLLGTEFQKKVWETTKTI 120
           +  LK     K     +   L  + + + +G I        E+L GT+FQ+KVW     +
Sbjct: 78  LQELKEAETYKKSIENYTICLE-NKMPLPSGAIPF------EFLFGTDFQRKVWNELLNV 130

Query: 121 KAGATISYQQLAVLLGNPKAVRAIGSALAKNNIAVVIPCHRIIGSKGKLTGFRWGIELKE 180
           + G  ++Y  +A  +G P A R++G A   NN+A+++PCHRI+GS  KLTG++W  +LKE
Sbjct: 131 EHGHVVTYGDIAKRIGKPTAARSVGRACGSNNLALLVPCHRIVGSNRKLTGYKWSCKLKE 190

Query: 181 SLLRQEQ 187
            LL  E+
Sbjct: 191 QLLNNEK 197


>CE22693 [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT/MGT1
           involved in DNA repair
          Length = 186

 Score = 90.5 bits (223), Expect = 1e-18
 Identities = 43/96 (44%), Positives = 60/96 (61%)

Query: 93  IDSQTNIKCEYLLGTEFQKKVWETTKTIKAGATISYQQLAVLLGNPKAVRAIGSALAKNN 152
           +DS   +   +   T F  +V+   + I  G T SY  +A  +GNP AVRA+ SA A+NN
Sbjct: 84  LDSTIKVNAVHKGDTAFGMQVYSAIQKIPKGETRSYSDIAREIGNPSAVRAVASACARNN 143

Query: 153 IAVVIPCHRIIGSKGKLTGFRWGIELKESLLRQEQA 188
           +A ++PCHR+IGS G L+G+RWGI  K  LL+ E A
Sbjct: 144 LAYIVPCHRVIGSTGNLSGYRWGIAKKRRLLQAEGA 179


>7298893 [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT/MGT1
           involved in DNA repair
          Length = 194

 Score = 83.2 bits (204), Expect = 2e-16
 Identities = 38/85 (44%), Positives = 55/85 (64%), Gaps = 1/85 (1%)

Query: 104 LLGTEFQKKVWETTKTIKAGATISYQQLAVLLGNPKAVRAIGSALAKNNIAVVIPCHRII 163
           + GT+FQ  VW     +K G T +Y QLA  +G P AVRA+ SA+AKN +A++IPCHR++
Sbjct: 108 IYGTDFQLSVWRALVHMKRGETCTYSQLAERMGRPTAVRAVASAVAKNELAILIPCHRVV 167

Query: 164 GSKGKLTGFRWGIELKESLLRQEQA 188
              G  + + WG  LK+ LL  E++
Sbjct: 168 SQNG-ASKYHWGAALKQLLLADEKS 191


>Hs4505177 [L] KOG4062 6-O-methylguanine-DNA methyltransferase MGMT/MGT1
           involved in DNA repair
          Length = 207

 Score = 79.0 bits (193), Expect = 4e-15
 Identities = 35/74 (47%), Positives = 49/74 (65%)

Query: 113 VWETTKTIKAGATISYQQLAVLLGNPKAVRAIGSALAKNNIAVVIPCHRIIGSKGKLTGF 172
           +W+  K +K G  ISYQQLA L GNPKA RA+G A+  N + ++IPCHR++ S G +  +
Sbjct: 99  LWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNY 158

Query: 173 RWGIELKESLLRQE 186
             G+ +KE LL  E
Sbjct: 159 SGGLAVKEWLLAHE 172


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.134    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,222,423
Number of Sequences: 60738
Number of extensions: 376521
Number of successful extensions: 944
Number of sequences better than 1.0e-05: 4
Number of HSP's better than  0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 938
Number of HSP's gapped (non-prelim): 4
length of query: 189
length of database: 30,389,216
effective HSP length: 100
effective length of query: 89
effective length of database: 24,315,416
effective search space: 2164072024
effective search space used: 2164072024
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)