ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV0513    suspect: LH   S     KOG1106  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV0513  179231  179794 188  
         (188 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOL146w [S] KOG1106 Uncharacterized conserved protein                 187   6e-48
SPAC227.16c [S] KOG1106 Uncharacterized conserved protein              80   1e-15

>YOL146w [S] KOG1106 Uncharacterized conserved protein
          Length = 236

 Score =  187 bits (476), Expect = 6e-48
 Identities = 93/196 (47%), Positives = 130/196 (65%), Gaps = 10/196 (5%)

Query: 1   MGYYDIDDILADGTRFPCRFNYDVPGLGYLEGNPGRPISKNAKLELPLWMVHLLAVVGAQ 60
           MGYYDIDD+LADGT FPC+F YD+PGLGYLE NPGRPI+KN KL LPLW+  +LA+VG  
Sbjct: 43  MGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRPITKNTKLSLPLWLARILAIVG-- 100

Query: 61  SNEDSRXXXXXXXXXXXXXXXXAPRVLNAIKSGATSLDLHSISPHFYTLAEKWATLFSDK 120
            +E                   + +V+NAIK+   +LDLHSI+ HF++LA KW  LFS+K
Sbjct: 101 GDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDLHSINSHFFSLAIKWIMLFSEK 160

Query: 121 ELSQVLKDMLLERGQEINNHAISVSMFSNCNQTND--------SAVFLQTLDEFEKTIYR 172
           EL+ V+ ++LL+R QE+N+HA S+S+  N + T          ++ FL  L+E EK IY+
Sbjct: 161 ELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSANTNIATSTFLLKLEEMEKEIYK 220

Query: 173 DTNVNCKEMKKWLVLK 188
            ++ + K+ K+W+  K
Sbjct: 221 KSHESYKDTKRWMFKK 236


>SPAC227.16c [S] KOG1106 Uncharacterized conserved protein
          Length = 166

 Score = 80.5 bits (197), Expect = 1e-15
 Identities = 51/184 (27%), Positives = 84/184 (44%), Gaps = 23/184 (12%)

Query: 1   MGYYDIDDILADGTRFPCRFNYDVPGLGYLEGNPGRPISKNAKLELPLWMVHLLAVVGAQ 60
           M YYDID IL++  + PC     +PGLG+     GR +   +K+ELP W+  +LA+    
Sbjct: 1   MDYYDIDSILSENQKVPCTSTVSIPGLGH----EGRMVPTGSKVELPFWLAEVLAI---- 52

Query: 61  SNEDSRXXXXXXXXXXXXXXXXAPRVLNAIKSGATSLDLHSISPHFYTLAEKWATLFSDK 120
                                 +  V NA+K+   S+ +  I+ H+Y  AEK   L SD 
Sbjct: 53  ----------NSFVSIHMPAPFSSVVRNALKANPNSVSIRDITTHYYHFAEKMLHLISDD 102

Query: 121 ELSQVLKDMLLERGQEINNHAISVSMFSNCNQTNDSAVFLQTLDEFEKTIYRDTNVNCKE 180
            L Q+  + L  R   I + +++            ++ F++ LD+FEK I R ++   + 
Sbjct: 103 SLVQISLNTLRSRAMLIADASLNPQ-----GALQQNSQFIEGLDDFEKHILRVSHNAHRS 157

Query: 181 MKKW 184
           +  W
Sbjct: 158 LINW 161


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.135    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,651,484
Number of Sequences: 60738
Number of extensions: 389264
Number of successful extensions: 1012
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1006
Number of HSP's gapped (non-prelim): 2
length of query: 188
length of database: 30,389,216
effective HSP length: 100
effective length of query: 88
effective length of database: 24,315,416
effective search space: 2139756608
effective search space used: 2139756608
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)