ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV0533    good          H     KOG3243  Coenzyme transport and metabolism                              6,7-dimethyl-8-ribityllumazine synthase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV0533  185691 185191 -167 
         (167 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOL143c [H] KOG3243 67-dimethyl-8-ribityllumazine synthase            264   4e-71
SPBC409.13 [H] KOG3243 67-dimethyl-8-ribityllumazine synthase         146   1e-35
At2g44050 [H] KOG3243 67-dimethyl-8-ribityllumazine synthase           96   2e-20

>YOL143c [H] KOG3243 67-dimethyl-8-ribityllumazine synthase
          Length = 169

 Score =  264 bits (675), Expect = 4e-71
 Identities = 126/167 (75%), Positives = 149/167 (88%)

Query: 1   MAVKGLGQLDQVYDGSNLRIGIVYARWNRTIIDALVEGAIKRLQSLGVKEENIILESVPG 60
           MAVKGLG+ DQVYDGS +R+GI++ARWNR IIDALV+GAI+R+ SLGV+E NII+E+VPG
Sbjct: 1   MAVKGLGKPDQVYDGSKIRVGIIHARWNRVIIDALVKGAIERMASLGVEENNIIIETVPG 60

Query: 61  SFELPWGVERFVQKEFEKGHRLDAVIPIGVLIKGSTMHFEYISDSTTNAIMSLSSKLNTP 120
           S+ELPWG +RFV ++ + G  LD VIPIGVLIKGSTMHFEYISDSTT+A+M+L  K++ P
Sbjct: 61  SYELPWGTKRFVDRQAKLGKPLDVVIPIGVLIKGSTMHFEYISDSTTHALMNLQEKVDMP 120

Query: 121 VIFGLLTCLTEEQALARAGIDEGKTMHNHGEDWGAAAVEMAVKFGYN 167
           VIFGLLTC+TEEQALARAGIDE  +MHNHGEDWGAAAVEMAVKFG N
Sbjct: 121 VIFGLLTCMTEEQALARAGIDEAHSMHNHGEDWGAAAVEMAVKFGKN 167


>SPBC409.13 [H] KOG3243 67-dimethyl-8-ribityllumazine synthase
          Length = 159

 Score =  146 bits (369), Expect = 1e-35
 Identities = 82/153 (53%), Positives = 104/153 (67%), Gaps = 9/153 (5%)

Query: 15  GSNLRIGIVYARWNRTIIDALVEGAIKRL-QSLGVKEENIILESVPGSFELPWGVERFVQ 73
           G  LRI IV+ARWN   I+ LV+GA++ + +   VK ENI +ESVPGS+ELP G+   + 
Sbjct: 15  GPELRILIVHARWNLQAIEPLVKGAVETMIEKHDVKLENIDIESVPGSWELPQGIRASIA 74

Query: 74  KEFEKGHRLDAVIPIGVLIKGSTMHFEYISDSTTNAIMSLSSKLNTPVIFGLLTCLTEEQ 133
           +     +  DAVI IGVLIKGSTMHFEYIS++  + +M +      PVI GLLT L EEQ
Sbjct: 75  R-----NTYDAVIGIGVLIKGSTMHFEYISEAVVHGLMRVGLDSGVPVILGLLTVLNEEQ 129

Query: 134 ALARAGIDEGKTMHNHGEDWGAAAVEMAVKFGY 166
           AL RAG++ G   HNHG DWG+AAVEM +K  Y
Sbjct: 130 ALYRAGLNGG---HNHGNDWGSAAVEMGLKALY 159


>At2g44050 [H] KOG3243 67-dimethyl-8-ribityllumazine synthase
          Length = 227

 Score = 95.9 bits (237), Expect = 2e-20
 Identities = 58/153 (37%), Positives = 87/153 (55%), Gaps = 9/153 (5%)

Query: 15  GSNLRIGIVYARWNRTIIDALVEGAIKRLQSLGVKEENIILESVPGSFELPWGVERFVQK 74
           G  LR  IV AR+N  +   L+EGAI+  +   V+EE+I +  VPGSFE+  GV   V +
Sbjct: 82  GEGLRFAIVVARFNEVVTKLLLEGAIETFKKYSVREEDIEVIWVPGSFEI--GV---VAQ 136

Query: 75  EFEKGHRLDAVIPIGVLIKGSTMHFEYISDSTTNAIMSLSSKLNTPVIFGLLTCLTEEQA 134
              K  +  AV+ IG +I+G T H++ +++S  + ++S S     P IFG+LTC   +QA
Sbjct: 137 NLGKSGKFHAVLCIGAVIRGDTTHYDAVANSAASGVLSASINSGVPCIFGVLTCEDMDQA 196

Query: 135 LARAGIDEGKTMHNHGEDWGAAAVEMAVKFGYN 167
           L R+G   G    N G +    A+EMA  F ++
Sbjct: 197 LNRSGGKAG----NKGAETALTALEMASLFEHH 225


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.319    0.137    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,132,540
Number of Sequences: 60738
Number of extensions: 342843
Number of successful extensions: 726
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 719
Number of HSP's gapped (non-prelim): 3
length of query: 167
length of database: 30,389,216
effective HSP length: 98
effective length of query: 69
effective length of database: 24,436,892
effective search space: 1686145548
effective search space used: 1686145548
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)