ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0649 good I KOG3035 Lipid transport and metabolism Isoamyl acetate-hydrolyzing esterase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0649 229377 228655 -241
(241 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR126c [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 254 8e-68
At5g62930 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 107 1e-23
Hs17435143 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 107 2e-23
At5g45920 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 102 4e-22
SPCC126.10 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 100 3e-21
At2g38180 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 95 1e-19
At3g11210 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase 78 1e-14
>YOR126c [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 238
Score = 254 bits (649), Expect = 8e-68
Identities = 118/236 (50%), Positives = 168/236 (71%), Gaps = 1/236 (0%)
Query: 3 LTYQKFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWAL 62
+ Y+KFLLFGDSITEF+FNTR D DQ++LGAALVN YTRK+DI+QRGF GY SRWAL
Sbjct: 1 MDYEKFLLFGDSITEFAFNTRPIEDGKDQYALGAALVNEYTRKMDILQRGFKGYTSRWAL 60
Query: 63 KLLPKILEQEQDVAISTLFFGSNDSCQHEQQNVPLPEFKENTIKLIQMMKNKGIKVVVVG 122
K+LP+IL+ E ++ ++T+F G+ND+C Q+VPLPEF +N +++ +MK+ I+ +++G
Sbjct: 61 KILPEILKHESNIVMATIFLGANDACSAGPQSVPLPEFIDNIRQMVSLMKSYHIRPIIIG 120
Query: 123 PALHDQDHWYSLKKPEVDKGYVRSNELYKQYSDXXXXXXXXXDVAFVNLYEAFKDQ-GDT 181
P L D++ W K E+ GY R+NE + YSD V FV L +AF+ + GD
Sbjct: 121 PGLVDREKWEKEKSEEIALGYFRTNENFAIYSDALAKLANEEKVPFVALNKAFQQEGGDA 180
Query: 182 WPELLCDGLHFTGKGYEVMFNEVLKAVNKRYPEYSPENVEYKLPNWRLVESNGSTL 237
W +LL DGLHF+GKGY++ +E+LK + YP+Y P+N++YKL +WR V +GS +
Sbjct: 181 WQQLLTDGLHFSGKGYKIFHDELLKVIETFYPQYHPKNMQYKLKDWRDVLDDGSNI 236
>At5g62930 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 242
Score = 107 bits (267), Expect = 1e-23
Identities = 74/232 (31%), Positives = 120/232 (50%), Gaps = 23/232 (9%)
Query: 7 KFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWALKLLP 66
+ +LFGDSIT SF + G+AL +AY+RK D+V RG+ GYN+RWAL LL
Sbjct: 4 EIVLFGDSITAQSFRSG---------GWGSALADAYSRKADVVVRGYGGYNTRWALFLLH 54
Query: 67 KI--LEQEQDVAISTLFFGSNDSC----QHEQQNVPLPEFKENTIKLIQMMK--NKGIKV 118
I L +T+FFG+ND+ ++Q+VP+ E+ +N K++Q +K + + +
Sbjct: 55 HIFPLGSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLI 114
Query: 119 VVVGPALHD----QDHWYSLKKPEVDKGYVRSNELYKQYSDXXXXXXXXXDVAFVNLYEA 174
V++ P D Q + S+ + K R+NE Y+ + VNL+
Sbjct: 115 VLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSK 174
Query: 175 FKDQGDTWPELLCDGLHFTGKGYEVMFNEVLKAVNKRYPEYSPENVEYKLPN 226
++ D + L DGLH T +G V+F+EV + + + SPE + + P+
Sbjct: 175 MQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAW--LSPEEMPFDFPH 224
>Hs17435143 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 248
Score = 107 bits (266), Expect = 2e-23
Identities = 72/238 (30%), Positives = 124/238 (51%), Gaps = 29/238 (12%)
Query: 2 ALTYQKFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWA 61
AL + + LLFGDSIT+FSF Q GA+L + RK D++ RGFSGYN+RWA
Sbjct: 12 ALLWPRLLLFGDSITQFSFQ---------QGGWGASLADRLVRKCDVLNRGFSGYNTRWA 62
Query: 62 LKLLPKILEQEQDVAIS---TLFFGSNDSC---QHEQQNVPLPEFKENTIKLIQMMKNKG 115
+LP+++ + + I T+FFG+NDS ++ +Q++PL E+ N ++Q +K+
Sbjct: 63 KIILPRLIRKGNSLDIPVAVTIFFGANDSALKDENPKQHIPLEEYAANLKSMVQYLKSVD 122
Query: 116 I---KVVVVGPALHDQDHWYSLKKPEVDKG--YVRSNELYKQYSDXXXXXXXXXDVAFVN 170
I +V+++ P + W ++ + +G R N + +Y++ ++
Sbjct: 123 IPENRVILITPTPLCETAW---EEQCIIQGCKLNRLNSVVGEYANACLQVAQDCGTDVLD 179
Query: 171 LYEAFKDQGDTWPELLCDGLHFTGKGYEVMFNEVLKAVNKRYPEYSPENVEYKLPNWR 228
L+ +D D + L DGLH + KG E +F+ + + K+ ++ LP WR
Sbjct: 180 LWTLMQDSQD-FSSYLSDGLHLSPKGNEFLFSHLWPLIEKKV-----SSLPLLLPYWR 231
>At5g45920 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 240
Score = 102 bits (255), Expect = 4e-22
Identities = 67/217 (30%), Positives = 111/217 (50%), Gaps = 21/217 (9%)
Query: 6 QKFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWALKLL 65
+K LFGDSITE SF+ GA+L + RK D+V RG+SGYN+RWALK++
Sbjct: 3 RKIFLFGDSITEESFSDG---------GWGASLADLLRRKADMVLRGYSGYNTRWALKVV 53
Query: 66 PKILEQEQD-----VAISTLFFGSNDSCQHEQ----QNVPLPEFKENTIKLIQMMKNKGI 116
++ ++ A T+FFG+ND+C E+ Q+VPL E+K+N ++ +KN+
Sbjct: 54 ERVFPVAEEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWP 113
Query: 117 KVVVVGPALHDQDHWYSLKKPEVDKGY---VRSNELYKQYSDXXXXXXXXXDVAFVNLYE 173
+ ++ D L+ P ++ R+NE+ Y+ ++ +L+
Sbjct: 114 QTAIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWS 173
Query: 174 AFKDQGDTWPELLCDGLHFTGKGYEVMFNEVLKAVNK 210
+ + E L DGLH + G +V+F EV K + +
Sbjct: 174 KMQQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKE 210
>SPCC126.10 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 246
Score = 99.8 bits (247), Expect = 3e-21
Identities = 70/227 (30%), Positives = 110/227 (47%), Gaps = 27/227 (11%)
Query: 5 YQKFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWALKL 64
+ + + GDSIT+ F A L+N Y R+L + GFSGY SR L+
Sbjct: 37 FNRLTIIGDSITQKGFTPG---------GYCAELMNFYQRRLRVDVWGFSGYTSRHVLRY 87
Query: 65 LPKILEQEQDVAISTLFFGSNDSCQHEQQNV--PLPEFKENTIKLIQMMKNKGIKVVVVG 122
LP+I + + +F G+ND CQ + P+ EFK N + L + + K+++V
Sbjct: 88 LPEIPLEIDSTKLLIVFLGTND-CQLTETGYMCPVDEFKNNLLALTRPFPHS--KIIIVS 144
Query: 123 PALHDQDHWYSLKK-PEVDKGYVRSNELYKQYSDXXXXXXXXXDVAFVNLYEAFKDQGDT 181
P + +D + ++ P V N L K ++ +NLYE K
Sbjct: 145 PGICTKDICFKREQEPYVIAASETVNTLNKSKANSA---------GLINLYEITKSYPT- 194
Query: 182 WPELL-CDGLHFTGKGYEVMFNEVLKAVNKRYPEYSPENVEYKLPNW 227
PELL DGLHF+ GY ++FNE++ ++K +PE P N+ + P+W
Sbjct: 195 -PELLFTDGLHFSSLGYSLLFNEIVATISKAWPELLPNNLPLQFPHW 240
>At2g38180 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 312
Score = 94.7 bits (234), Expect = 1e-19
Identities = 66/228 (28%), Positives = 116/228 (49%), Gaps = 24/228 (10%)
Query: 7 KFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWALKLLP 66
+ +LFG SI ++SF R GA L + Y+R DI+ RG++G+NSR+ALK+L
Sbjct: 8 QIVLFGSSIVQYSFTDR---------GWGATLADLYSRTADIILRGYAGWNSRFALKVLH 58
Query: 67 KILEQEQDV--AISTLFFGSNDSCQHEQQ----NVPLPEFKENTIKL---IQMMKNKGIK 117
++ ++ + ++ ++FG NDS +VPL EF EN K+ + + +K
Sbjct: 59 QVFPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRV 118
Query: 118 VVVVGPALHDQDHWYSLKKPEVDKGYVRSNELYKQYSDXXXXXXXXXDVAFVNLYEAFKD 177
+ + P ++++ + + KG RSNEL + Y++ +V ++++ A +
Sbjct: 119 IFLTPPPMNEKQ--IEIVFGDAIKG--RSNELCRPYAEELLNLCREINVKGIDIWTAIQQ 174
Query: 178 QGDTWPELLCDGLHFTGKGYEVMFNEVLKAVNKRYPEYSPENVEYKLP 225
Q D DG+HFT K E++ E+LK + R ++ P LP
Sbjct: 175 QDDWLNSCFTDGIHFTAKASEIVVKEILKVL--RGADWKPSLYWKSLP 220
>At3g11210 [I] KOG3035 Isoamyl acetate-hydrolyzing esterase
Length = 256
Score = 78.2 bits (191), Expect = 1e-14
Identities = 54/212 (25%), Positives = 102/212 (47%), Gaps = 18/212 (8%)
Query: 7 KFLLFGDSITEFSFNTRMNGDQTDQFSLGAALVNAYTRKLDIVQRGFSGYNSRWALKLLP 66
+ +LFG SI + SF GA L Y RK DI+ RG+ G+NS AL+++
Sbjct: 8 QIVLFGSSIVQMSFG---------HGGWGAILSEVYARKADIILRGYYGWNSSRALEVVD 58
Query: 67 KILEQEQDV--AISTLFFGSNDSCQHEQQ----NVPLPEFKENTIKLIQMMKNKG--IKV 118
++ ++ V ++ ++FG NDS +VPL E+ +N K+ +++ ++
Sbjct: 59 QVFPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRI 118
Query: 119 VVVGPALHDQDHWYSLKKPEVDKGYVRSNELYKQYSDXXXXXXXXXDVAFVNLYEAFKDQ 178
+ + D+ + P + + +R+N+L K YSD + V+L+ F+
Sbjct: 119 IFLSSPPVDEAKVRQNQSPYLSE-VIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKA 177
Query: 179 GDTWPELLCDGLHFTGKGYEVMFNEVLKAVNK 210
D DG+H + +G +++ E+L+ V +
Sbjct: 178 DDWKTVCFTDGIHLSAQGSKIVAGEILRVVKE 209
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,342,010
Number of Sequences: 60738
Number of extensions: 592573
Number of successful extensions: 1252
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1236
Number of HSP's gapped (non-prelim): 7
length of query: 241
length of database: 30,389,216
effective HSP length: 103
effective length of query: 138
effective length of database: 24,133,202
effective search space: 3330381876
effective search space used: 3330381876
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)