ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0681 suspect: HSP IM KOG2547 Cell wall/membrane/envelope biogenesis Ceramide glucosyltransferase
r_klactV0681 suspect: HSP IM KOG2547 Lipid transport and metabolism Ceramide glucosyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0681 239180 240835 552
(552 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
Hs4507811 [IM] KOG2547 Ceramide glucosyltransferase 122 1e-27
7291367 [IM] KOG2547 Ceramide glucosyltransferase 98 3e-20
CE16360 [IM] KOG2547 Ceramide glucosyltransferase 88 3e-17
CE26371 [IM] KOG2547 Ceramide glucosyltransferase 80 1e-14
CE07316 [IM] KOG2547 Ceramide glucosyltransferase 80 1e-14
CE07105 [IM] KOG2547 Ceramide glucosyltransferase 79 1e-14
>Hs4507811 [IM] KOG2547 Ceramide glucosyltransferase
Length = 394
Score = 122 bits (306), Expect = 1e-27
Identities = 72/204 (35%), Positives = 116/204 (56%), Gaps = 16/204 (7%)
Query: 87 KELPEEDLAKLEPVSILRPCKGVDSEMVACLESCINQDYPKHLFEVIFCVESSTDSSIAI 146
K ++ +KL VS+L+P KGVD ++ LE+ DYPK +EV+ CV+ D +I +
Sbjct: 40 KATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPK--YEVLLCVQDHDDPAIDV 97
Query: 147 IQKILAKHPDHNLSLLIGDKDYFGPNPKINNLSKGYRMAKYDIVWVLDSNVWCSPGTLAR 206
+K+L K+P+ + L IG K G NPKINNL GY +AKYD++W+ DS + P TL
Sbjct: 98 CKKLLGKYPNVDARLFIGGKKV-GINPKINNLMPGYEVAKYDLIWICDSGIRVIPDTLTD 156
Query: 207 SVSSLTKSLDNGIRTSKPVVLTHHVPLGISINSESVSGRLDEMFLFSSHAKFYVAFNYVS 266
V+ +T+ V L H +P + + + L++++ +SH ++Y++ N V+
Sbjct: 157 MVNQMTEK----------VGLVHGLP--YVADRQGFAATLEQVYFGTSHPRYYISAN-VT 203
Query: 267 IAPCVNGKSNLYRRSNLEKAVQMI 290
CV G S L R+ L++A +I
Sbjct: 204 GFKCVTGMSCLMRKDVLDQAGGLI 227
Score = 53.1 bits (126), Expect = 1e-06
Identities = 46/196 (23%), Positives = 82/196 (41%), Gaps = 33/196 (16%)
Query: 357 FSTYIGEDNMIGTALWDMLQGRTGMTRDVVIQPLKFGSSDDSWHSYITRRVRWLRVRKYM 416
F+ YI ED + A+ D R M+ V +Q S S + +R +RW ++R M
Sbjct: 229 FAQYIAEDYFMAKAIADR-GWRFAMSTQVAMQ----NSGSYSISQFQSRMIRWTKLRINM 283
Query: 417 VLAATLLEPTTESILIGTMGLFGLTRLLPGSVSFWKAFIIHMFLWCLSDWIQYRTLCQNT 476
+ A + EP +E + + + + + + F+ H W + D+IQ R +
Sbjct: 284 LPATIICEPISECFVASLIIGWAAHHVFRWDIMVF--FMCHCLAWFIFDYIQLRGV---- 337
Query: 477 VNDNVMNRSSIFSDPGYTYPFFTPGLHPRSFTDWIGTWILRECLALPVWINAMCGSVIQW 536
G T F S D+ W +RE + + ++++A+ I W
Sbjct: 338 --------------QGGTLCF--------SKLDYAVAWFIRESMTIYIFLSALWDPTISW 375
Query: 537 RNRPFRIKPDLTAEEL 552
R +R++ TAEE+
Sbjct: 376 RTGRYRLRCGGTAEEI 391
>7291367 [IM] KOG2547 Ceramide glucosyltransferase
Length = 440
Score = 98.2 bits (243), Expect = 3e-20
Identities = 63/198 (31%), Positives = 106/198 (52%), Gaps = 17/198 (8%)
Query: 88 ELPEEDLAKLEPVSILRPCKGVDSEMVACLESCINQDYPKHLFEVIFCVESSTDSSIAII 147
+LP E L VSIL+P GVD + LE+ DYP L+E++FCVE D +I ++
Sbjct: 43 KLPTE-AQPLPGVSILKPLMGVDPNLQHNLETFFTMDYP--LYELLFCVEDKEDPAIQLV 99
Query: 148 QKILAKHPDHNLSLLIGDKDYFGPNPKINNLSKGYRMAKYDIVWVLDSNVWCSPGTLARS 207
+++LAK+P + +L +G D G NPKINN+ GY AKYD V + DS + TL
Sbjct: 100 ERLLAKYPLVDAALFVGGSDV-GVNPKINNIHPGYMAAKYDFVMISDSGIKMKDDTLLDM 158
Query: 208 VSSLTKSLDNGIRTSKPVVLTHHVPLGISINSESVSGRLDEMFLFSSHAKFYVAFNYVSI 267
V ++ S+ L H +P + + + + +++F + ++ Y++ + + I
Sbjct: 159 VQNM----------SEKHALVHQMP--FTCDRDGFAATFEKVFFGTVQSRIYLSADVLGI 206
Query: 268 APCVNGKSNLYRRSNLEK 285
C G S L R++ +++
Sbjct: 207 -NCHTGMSCLLRKAVIDQ 223
>CE16360 [IM] KOG2547 Ceramide glucosyltransferase
Length = 422
Score = 88.2 bits (217), Expect = 3e-17
Identities = 50/194 (25%), Positives = 97/194 (49%), Gaps = 24/194 (12%)
Query: 97 LEPVSILRPCKGVDSEMVACLESCINQDYPKHLFEVIFCVESSTDSSIAIIQKILAKHPD 156
L VS+++P G D + LES Y H FE++FC S D +I +++ ++ KHP+
Sbjct: 106 LPGVSVIKPIVGTDKNLYQNLESFFTSQY--HSFELLFCFHSEEDEAIEVVRSLIKKHPN 163
Query: 157 HNLSLLIGDKDYFGPNPKINNLSKGYRMAKYDIVWVLDSNVWCSPGTLARSVSSLTKSLD 216
+L + + G NPK+NN+ YR A+Y +V + DS ++ P
Sbjct: 164 IEAKILF-EGEPVGMNPKVNNMMPAYRAARYPLVLISDSAIFMRP--------------- 207
Query: 217 NGIRTSKPVVLTHHVPLGIS-----INSESVSGRLDEMFLFSSHAKFYVAFNYVSIAPCV 271
+GI +++H ++ + + +++F +SHA+ Y+ N++ + C
Sbjct: 208 DGILDMATTMMSHEKMASVTQIPYCKDRQGFHAAFEQIFFGTSHARLYLVGNFLGVV-CS 266
Query: 272 NGKSNLYRRSNLEK 285
+G S++ ++S L++
Sbjct: 267 SGMSSMMKKSALDE 280
>CE26371 [IM] KOG2547 Ceramide glucosyltransferase
Length = 522
Score = 79.7 bits (195), Expect = 1e-14
Identities = 51/192 (26%), Positives = 95/192 (48%), Gaps = 16/192 (8%)
Query: 95 AKLEPVSILRPCKGVDSEMVACLESCINQDYPKHLFEVIFCVESSTDSSIAIIQKILAKH 154
+ L VSI++P G D+ + +ES Y H +E++FC SS D ++ +++ ++ K+
Sbjct: 123 SSLPGVSIIKPIVGKDNNLYENIESFFTTQY--HKYELLFCFNSSDDEAVEVVKCLMKKY 180
Query: 155 PDHNLSLLIGDKDYFGPNPKINNLSKGYRMAKYDIVWVLDSNVWC-SPGTLARSVSSLTK 213
P + L G + G NPKINN+ YR A Y ++ V DS ++ S G L + + +
Sbjct: 181 PKVDAKLFFGGETV-GLNPKINNMMPAYRSALYPLILVSDSGIFMRSDGVLDMATTMM-- 237
Query: 214 SLDNGIRTSKPVVLTHHVPLGISINSESVSGRLDEMFLFSSHAKFYVAFNYVSIAPCVNG 273
+ + + L P + E ++M+ +SH + Y+A N + C G
Sbjct: 238 -------SHEKMALVTQTP--YCKDREGFDAAFEQMYFGTSHGRIYLAGNCMDFV-CSTG 287
Query: 274 KSNLYRRSNLEK 285
S++ ++ L++
Sbjct: 288 MSSMMKKEALDE 299
>CE07316 [IM] KOG2547 Ceramide glucosyltransferase
Length = 511
Score = 79.7 bits (195), Expect = 1e-14
Identities = 51/192 (26%), Positives = 95/192 (48%), Gaps = 16/192 (8%)
Query: 95 AKLEPVSILRPCKGVDSEMVACLESCINQDYPKHLFEVIFCVESSTDSSIAIIQKILAKH 154
+ L VSI++P G D+ + +ES Y H +E++FC SS D ++ +++ ++ K+
Sbjct: 112 SSLPGVSIIKPIVGKDNNLYENIESFFTTQY--HKYELLFCFNSSDDEAVEVVKCLMKKY 169
Query: 155 PDHNLSLLIGDKDYFGPNPKINNLSKGYRMAKYDIVWVLDSNVWC-SPGTLARSVSSLTK 213
P + L G + G NPKINN+ YR A Y ++ V DS ++ S G L + + +
Sbjct: 170 PKVDAKLFFGGETV-GLNPKINNMMPAYRSALYPLILVSDSGIFMRSDGVLDMATTMM-- 226
Query: 214 SLDNGIRTSKPVVLTHHVPLGISINSESVSGRLDEMFLFSSHAKFYVAFNYVSIAPCVNG 273
+ + + L P + E ++M+ +SH + Y+A N + C G
Sbjct: 227 -------SHEKMALVTQTP--YCKDREGFDAAFEQMYFGTSHGRIYLAGNCMDFV-CSTG 276
Query: 274 KSNLYRRSNLEK 285
S++ ++ L++
Sbjct: 277 MSSMMKKEALDE 288
>CE07105 [IM] KOG2547 Ceramide glucosyltransferase
Length = 420
Score = 79.3 bits (194), Expect = 1e-14
Identities = 53/218 (24%), Positives = 102/218 (46%), Gaps = 26/218 (11%)
Query: 90 PEEDLAKLEPVSILRPCKGVDSEMVACLESCINQDYPKHLFEVIFCVESSTDSSIAIIQK 149
P + L VSI++P G D+ + LE+ Y H FE++FC + S D ++ +++
Sbjct: 90 PTKPNPSLPGVSIIKPIIGADANLYTNLETFFTTQY--HKFELLFCFDRSDDPAVKVVES 147
Query: 150 ILAKHPDHNLSLLIGDKDYFGPNPKINNLSKGYRMAKYDIVWVLDSNVWCSPGTLARSVS 209
++ K+P + ++ G + G NPKINN+ YR+AKY ++ + DS ++ + S
Sbjct: 148 LVKKYPSVDSTMFFGGEK-IGLNPKINNMMPAYRIAKYQLIMISDSGIFMKSDAVLDMAS 206
Query: 210 SLTKSLDNGIRTSKPV---------------VLTHHVPLGISIN-------SESVSGRLD 247
++ + T P +L + I +N + S+S
Sbjct: 207 TMMSHETMALVTQTPYCKDRKGKSKNCAPIQILKRLTLIFIVVNVLACFNIALSISVWAT 266
Query: 248 EMFLFSSHAKFYVAFNYVSIAPCVNGKSNLYRRSNLEK 285
++ +SHA+ Y+A N + C G S++ ++ L++
Sbjct: 267 LIYFGTSHARIYLAGNCLQF-NCPTGMSSMMKKEALDE 303
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.137 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,437,711
Number of Sequences: 60738
Number of extensions: 1349633
Number of successful extensions: 2605
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2579
Number of HSP's gapped (non-prelim): 14
length of query: 552
length of database: 30,389,216
effective HSP length: 111
effective length of query: 441
effective length of database: 23,647,298
effective search space: 10428458418
effective search space used: 10428458418
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)