ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0711 good EH KOG1184 Amino acid transport and metabolism Thiamine pyrophosphate-requiring enzyme
r_klactV0711 good EH KOG1184 Coenzyme transport and metabolism Thiamine pyrophosphate-requiring enzyme
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0711 251220 253112 631
(631 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR380w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 583 e-166
YLR044c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 275 2e-73
YLR134w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 270 6e-72
YGR087c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 269 8e-72
YDL080c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 260 5e-69
SPAC3G9.11c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 246 8e-65
At5g54960 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 159 1e-38
At5g01320 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 153 7e-37
At5g01330 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 152 1e-36
At4g33070 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 150 6e-36
SPAC186.09 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 146 1e-34
SPAC1F8.07c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 143 7e-34
SPAC13A11.06 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme 117 7e-26
At3g48560 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 71 4e-12
SPBP35G2.07 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme 66 2e-10
>YDR380w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 635
Score = 583 bits (1502), Expect = e-166
Identities = 292/603 (48%), Positives = 396/603 (65%), Gaps = 11/603 (1%)
Query: 32 IPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAAD 91
IPFG Y+F+RLL+ KS+FGVPGDFNL + GL+W+GTCNELN AYAAD
Sbjct: 25 IPFGEYIFKRLLSIDTKSVFGVPGDFNLSLLEYLYSPSVESAGLRWVGTCNELNAAYAAD 84
Query: 92 GYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVH 151
GYSRY+NK+GC+ITTYGVGELSALNG+AG+FAE+VKVLHI+GV+ S + F N+H
Sbjct: 85 GYSRYSNKIGCLITTYGVGELSALNGIAGSFAENVKVLHIVGVAKSIDSRSSNFSDRNLH 144
Query: 152 HLVPAIDSQ--QPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYV 209
HLVP + + PNH+VY DMVKDRV CS A+L+DI TA D++D VI DI K SKPGY+
Sbjct: 145 HLVPQLHDSNFKGPNHKVYHDMVKDRVACSVAYLEDIETACDQVDNVIRDIYKYSKPGYI 204
Query: 210 FIPVDFADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDL 269
F+P DFAD V+ NL+ P I I Y EN +++ WIY S P++ D+
Sbjct: 205 FVPADFADMSVTCDNLVNVPRISQQDCI-VYPSENQLSDIINKITSWIYSSKTPAILGDV 263
Query: 270 LVDRFNLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEMQATVDSSD 329
L DR+ + L + I +WN++T MGKS +DE NP Y+G Y G E K++ + D
Sbjct: 264 LTDRYGVSNFLNKLICKTGIWNFSTVMGKSVIDESNPTYMGQYNGKEGLKQVYEHFELCD 323
Query: 330 LILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVLKYV 389
L+LHFG NEIN G+Y+F + NA+II+ N I+ +D + N + NF +LK +
Sbjct: 324 LVLHFGVDINEINNGHYTFTYKPNAKIIQFHPNYIRLVDTRQGNEQMFKGINFAPILKEL 383
Query: 390 NDNLDKSKLSFSYPP--VPKSNHSCEYDN-----NDSITQKSLKEIIPNYFNDGDVLIVE 442
+D SKLS Y +N + ++ + ITQ L++ +P + N GDV++ E
Sbjct: 384 YKRIDVSKLSLQYDSNVTQYTNETMRLEDPTNGQSSIITQVHLQKTMPKFLNPGDVVVCE 443
Query: 443 TGSFQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDY 502
TGSFQF V + F ++KYI+Q F+LSIGMALPAALGVG M+D+ +HI + V +DY
Sbjct: 444 TGSFQFSVRDFAFPSQLKYISQGFFLSIGMALPAALGVGIAMQDHSNAHI-NGGNVKEDY 502
Query: 503 IPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAINGPTRSYNDIAPFK 562
PRLIL EGDGAAQMTIQE ++ ++ IP+E+++WNNNGYT+ERAI GPTRSYND+ +K
Sbjct: 503 KPRLILFEGDGAAQMTIQELSTILKCNIPLEVIIWNNNGYTIERAIMGPTRSYNDVMSWK 562
Query: 563 WTALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQELN 622
WT L FGDF+ K+ S ++ +L L + K+ +R+ I+L EV L +D P++L
Sbjct: 563 WTKLFEAFGDFDGKYTNSTLIQCPSKLALKLEELKNSNKRSGIELLEVKLGELDFPEQLK 622
Query: 623 AML 625
M+
Sbjct: 623 CMV 625
>YLR044c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 275 bits (702), Expect = 2e-73
Identities = 192/602 (31%), Positives = 305/602 (49%), Gaps = 62/602 (10%)
Query: 29 VKNIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAY 88
+ I G Y+FERL ++FG+PGDFNL G++W G NELN AY
Sbjct: 1 MSEITLGKYLFERLKQVNVNTVFGLPGDFNLSLLDKIYE----VEGMRWAGNANELNAAY 56
Query: 89 AADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSH 148
AADGY+R + C+ITT+GVGELSALNG+AG++AE V VLH++GV PS + +Q
Sbjct: 57 AADGYARIKG-MSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGV-PSISAQAKQLL-- 112
Query: 149 NVHHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGY 208
+HH + D F + + ++A + DI TA +D+ I +P Y
Sbjct: 113 -LHHTLGNGDF-------TVFHRMSANISETTAMITDIATAPAEIDRCIRTTYVTQRPVY 164
Query: 209 VFIPVDFADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSD 268
+ +P + D V K L+Q PI DM D + K D +L + ++ P + +D
Sbjct: 165 LGLPANLVDLNVPAK-LLQTPI-DMSL---KPNDAESEKEVIDTILALVKDAKNPVILAD 219
Query: 269 LLVDRFNLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEMQATVDSS 328
R +++ E ++ I+ + T MGK ++DE++P+Y G+Y G + E++ V+S+
Sbjct: 220 ACCSRHDVKAETKKLIDLTQFPAFVTPMGKGSIDEQHPRYGGVYVGTLSKPEVKEAVESA 279
Query: 329 DLILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSV-LK 387
DLIL G ++ N G +S+ + + NI++F + + A F V +K
Sbjct: 280 DLILSVGALLSDFNTGSFSYSYK--------TKNIVEF----HSDHMKIRNATFPGVQMK 327
Query: 388 YVNDNL-----DKSKLSFSYPPVP---KSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVL 439
+V L D +K Y PV ++ + + + Q+ + + N+ +GDV+
Sbjct: 328 FVLQKLLTTIADAAK---GYKPVAVPARTPANAAVPASTPLKQEWMWNQLGNFLQEGDVV 384
Query: 440 IVETGSFQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVP 499
I ETG+ FG+ F I+Q + SIG A LG + + P
Sbjct: 385 IAETGTSAFGINQTTFPNNTYGISQVLWGSIGFTTGATLGAAFAAEE----------IDP 434
Query: 500 KDYIPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAINGPTRSYNDIA 559
K R+IL GDG+ Q+T+QE ++ IR+ + + V NN+GYT+E+ I+GP YN+I
Sbjct: 435 KK---RVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIEKLIHGPKAQYNEIQ 491
Query: 560 PFKWTALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQ 619
+ +LL FG + + + T + D+L Q KS + + I++ E+MLPV D PQ
Sbjct: 492 GWDHLSLLPTFGAKDYETHRVATTGEWDKLTQ----DKSFNDNSKIRMIEIMLPVFDAPQ 547
Query: 620 EL 621
L
Sbjct: 548 NL 549
>YLR134w [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 270 bits (689), Expect = 6e-72
Identities = 188/596 (31%), Positives = 305/596 (50%), Gaps = 50/596 (8%)
Query: 29 VKNIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAY 88
+ I G Y+FERL ++FG+PGDFNL G++W G NELN AY
Sbjct: 1 MSEITLGKYLFERLSQVNCNTVFGLPGDFNLSLLDKLYE----VKGMRWAGNANELNAAY 56
Query: 89 AADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSH 148
AADGY+R + C+ITT+GVGELSALNG+AG++AE V VLH++GV PS + +Q
Sbjct: 57 AADGYARIKG-MSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGV-PSISSQAKQLL-- 112
Query: 149 NVHHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGY 208
+HH + D F + + ++A + DI A +D+ I +P Y
Sbjct: 113 -LHHTLGNGDF-------TVFHRMSANISETTAMITDIANAPAEIDRCIRTTYTTQRPVY 164
Query: 209 VFIPVDFADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSD 268
+ +P + D V K L++ PI D ++ E ++ +++ I ++ P + +D
Sbjct: 165 LGLPANLVDLNVPAK-LLETPI---DLSLKPNDAEAEAEVVRT-VVELIKDAKNPVILAD 219
Query: 269 LLVDRFNLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEMQATVDSS 328
R +++ E ++ ++ Y T MGK +DE++P+Y G+Y G + E++ V+S+
Sbjct: 220 ACASRHDVKAETKKLMDLTQFPVYVTPMGKGAIDEQHPRYGGVYVGTLSRPEVKKAVESA 279
Query: 329 DLILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFID--IKDKNASTVEEANFVSVL 386
DLIL G ++ N G +S+ + + NI++F IK +NA T L
Sbjct: 280 DLILSIGALLSDFNTGSFSYSYK--------TKNIVEFHSDHIKIRNA-TFPGVQMKFAL 330
Query: 387 KYVNDNLDKSKLSFSYPPVP-KSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVETGS 445
+ + D + + + VP + + N + Q+ + + N+ +GD++I ETG+
Sbjct: 331 QKLLDAIPEVVKDYKPVAVPARVPITKSTPANTPMKQEWMWNHLGNFLREGDIVIAETGT 390
Query: 446 FQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIPR 505
FG+ F ++ I Q + SIG + A LG + + + L PK R
Sbjct: 391 SAFGINQTTFPTDVYAIVQVLWGSIGFTVGALLG----------ATMAAEELDPKK---R 437
Query: 506 LILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAINGPTRSYNDIAPFKWTA 565
+IL GDG+ Q+T+QE ++ IR+ + I V NNNGYT+E+ I+GP YN+I + A
Sbjct: 438 VILFIGDGSLQLTVQEISTMIRWGLKPYIFVLNNNGYTIEKLIHGPHAEYNEIQGWDHLA 497
Query: 566 LLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
LL FG + + T + ++L Q K ++ + I++ EVMLPV D PQ L
Sbjct: 498 LLPTFGARNYETHRVATTGEWEKLTQ----DKDFQDNSKIRMIEVMLPVFDAPQNL 549
>YGR087c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 563
Score = 269 bits (688), Expect = 8e-72
Identities = 189/599 (31%), Positives = 305/599 (50%), Gaps = 56/599 (9%)
Query: 29 VKNIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAY 88
+ I G Y+FERL +IFG+PGDFNL +GL+W G NELN AY
Sbjct: 1 MSEITLGKYLFERLKQVNVNTIFGLPGDFNLSLLDKIYE----VDGLRWAGNANELNAAY 56
Query: 89 AADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSH 148
AADGY+R L ++TT+GVGELSALNG+AG++AE V VLH++GV PS + +Q
Sbjct: 57 AADGYARIKG-LSVLVTTFGVGELSALNGIAGSYAEHVGVLHVVGV-PSISAQAKQLL-- 112
Query: 149 NVHHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGY 208
+HH + D F + + +++ + DI TA +D++I +P Y
Sbjct: 113 -LHHTLGNGDF-------TVFHRMSANISETTSMITDIATAPSEIDRLIRTTFITQRPSY 164
Query: 209 VFIPVDFADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSD 268
+ +P + D V +L++ PI D ++ D A K D +L+ I S P + SD
Sbjct: 165 LGLPANLVDLKVPG-SLLEKPI---DLSLKP-NDPEAEKEVIDTVLELIQNSKNPVILSD 219
Query: 269 LLVDRFNLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEMQATVDSS 328
R N++KE ++ I+ + T +GK ++DE++P+Y G+Y G + ++++ V+S+
Sbjct: 220 ACASRHNVKKETQKLIDLTQFPAFVTPLGKGSIDEQHPRYGGVYVGTLSKQDVKQAVESA 279
Query: 329 DLILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSV-LK 387
DLIL G ++ N G +S+ + + N+++F + V+ A F+ V +K
Sbjct: 280 DLILSVGALLSDFNTGSFSYSYK--------TKNVVEF----HSDYVKVKNATFLGVQMK 327
Query: 388 YVNDNL-----DKSKLSFSYPPVPKSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVE 442
+ NL D K S P K+ + + + Q+ L + + +GDV+I E
Sbjct: 328 FALQNLLKVIPDVVKGYKSVPVPTKTPANKGVPASTPLKQEWLWNELSKFLQEGDVIISE 387
Query: 443 TGSFQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDY 502
TG+ FG+ F ++ I+Q + SIG A LG + D
Sbjct: 388 TGTSAFGINQTIFPKDAYGISQVLWGSIGFTTGATLGAAFAAEEI-------------DP 434
Query: 503 IPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAINGPTRSYNDIAPFK 562
R+IL GDG+ Q+T+QE ++ IR+ + + V NN+GYT+E+ I+GP YN+I +
Sbjct: 435 NKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIEKLIHGPHAEYNEIQTWD 494
Query: 563 WTALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
ALL FG + + ++ T + D L ++ + I+L E+ LPV D P+ L
Sbjct: 495 HLALLPAFGAKKYENHKIATTGEWD----ALTTDSEFQKNSVIRLIELKLPVFDAPESL 549
>YDL080c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 609
Score = 260 bits (664), Expect = 5e-69
Identities = 187/598 (31%), Positives = 302/598 (50%), Gaps = 49/598 (8%)
Query: 30 KNIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYA 89
K I Y+F RL +IFG+ G+F++P L+W G NELN AYA
Sbjct: 13 KCIAISDYLFHRLNQLNIHTIFGLSGEFSMPLLDKLYNIP----NLRWAGNSNELNAAYA 68
Query: 90 ADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHN 149
ADGYSR LGC+ITT+GVGELSA+NGVAG++AE V +LHI+G+ P+ + Q +
Sbjct: 69 ADGYSRLKG-LGCLITTFGVGELSAINGVAGSYAEHVGILHIVGMPPT----SAQTKQLL 123
Query: 150 VHHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYV 209
+HH + D F + V C + + D AD +D+ I +P Y+
Sbjct: 124 LHHTLGNGDF-------TVFHRIASDVACYTTLIIDSELCADEVDKCIKKAWIEQRPVYM 176
Query: 210 FIPVDFADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDL 269
+PV+ + + + L N +D+ D + K R+L +IY+S P++ D
Sbjct: 177 GMPVNQVNLPIESARL--NTPLDLQL---HKNDPDVEKEVISRILSFIYKSQNPAIIVDA 231
Query: 270 LVDRFNLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEMQATVDSSD 329
R NL +E ++ + T MGK T++E +P++ G++ G+ + E++ VD +D
Sbjct: 232 CTSRQNLIEETKELCNRLKFPVFVTPMGKGTVNETDPQFGGVFTGSISAPEVREVVDFAD 291
Query: 330 LILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVLKYV 389
I+ G +E + +FHF + L + +K KNA T + + +L+ +
Sbjct: 292 FIIVIGCMLSEFSTS--TFHFQYKTKNCAL----LYSTSVKLKNA-TYPDLSIKLLLQKI 344
Query: 390 NDNLDKSKLSFSYPPVPKSNHSCEYD-NNDSITQKSLKEIIPNYFNDGDVLIVETGSFQF 448
NLD+SKLS+ P Y N + Q+ + I ++F GD++I ETG+ F
Sbjct: 345 LANLDESKLSYQPSEQPSMMVPRPYPAGNVLLRQEWVWNEISHWFQPGDIIITETGASAF 404
Query: 449 GVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIP---- 504
GV +F I+Q+ + S+G + A LG +++ + KD P
Sbjct: 405 GVNQTRFPVNTLGISQALWGSVGYTMGACLGAEFAVQE-----------INKDKFPATKH 453
Query: 505 RLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAINGPT-RSYNDIAPFKW 563
R+IL GDGA Q+T+QE ++ +++ + I V NN GY+V+R ++ + SY DI P+ +
Sbjct: 454 RVILFMGDGAFQLTVQELSTIVKWGLTPYIFVMNNQGYSVDRFLHHRSDASYYDIQPWNY 513
Query: 564 TALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
LL VFG + + +TV + ++ N + K I++ E+MLP D+PQ L
Sbjct: 514 LGLLRVFGCTNYETKKIITVGEFRSMISDPNFATNDK----IRMIEIMLPPRDVPQAL 567
>SPAC3G9.11c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 570
Score = 246 bits (628), Expect = 8e-65
Identities = 177/590 (30%), Positives = 289/590 (48%), Gaps = 44/590 (7%)
Query: 35 GHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAADGYS 94
G Y+F RLL G KSI GVPGDFNL +W+G NELNGAYAAD Y+
Sbjct: 9 GEYLFTRLLQLGIKSILGVPGDFNLALLDLIEKVGDET--FRWVGNENELNGAYAADAYA 66
Query: 95 RYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVHHLV 154
R + ++TT+GVGELSALNG AGA++E + V+HI+GV ++ + +HH +
Sbjct: 67 RVKG-ISAIVTTFGVGELSALNGFAGAYSERIPVVHIVGVPNTKAQATRPL----LHHTL 121
Query: 155 PAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYVFIPVD 214
D + F + + AFLD ++A +D ++ + S+P Y+ +P D
Sbjct: 122 GNGDFK-------VFQRMSSELSADVAFLDSGDSAGRLIDNLLETCVRTSRPVYLAVPSD 174
Query: 215 FADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDLLVDRF 274
A ++ + ++ P++ V E+ K+ ++ +L+ I +S PS+ D V RF
Sbjct: 175 -AGYFYTDASPLKTPLVF--PVPENNKEIEHEVVS--EILELIEKSKNPSILVDACVSRF 229
Query: 275 NLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEMQATVDSSDLILHF 334
++++E + FI+ Y T MGK+ ++E +P + G+Y G+ T ++ +S+DL+L
Sbjct: 230 HIQQETQDFIDATHFPTYVTPMGKTAINESSPYFDGVYIGSLTEPSIKERAESTDLLLII 289
Query: 335 GPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVLKYVNDNLD 394
G +++ N G +++ S I D + E + +L + +D
Sbjct: 290 GGLRSDFNSGTFTY-------ATPASQTIEFHSDYTKIRSGVYEGISMKHLLPKLTAAID 342
Query: 395 KSKLSFSYPPV---PKSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVETGSFQFGVP 451
K + PV P + E +IT K ++ + DV+ ETG+ FG+
Sbjct: 343 KKSVQAKARPVHFEPPKAVAAEGYAEGTITHKWFWPTFASFLRESDVVTTETGTSNFGIL 402
Query: 452 NMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIPRLILCEG 511
+ F + + ++Q + SIG ++ A G G++D H R IL G
Sbjct: 403 DCIFPKGCQNLSQVLWGSIGWSVGAMFGATLGIKDSDAPH------------RRSILIVG 450
Query: 512 DGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAINGPTRSYNDIAPFKWTALLNVFG 571
DG+ +T+QE ++ IR + I V NN GYT+ER I+G YNDI +W N+
Sbjct: 451 DGSLHLTVQEISATIRNGLTPIIFVINNKGYTIERLIHGLHAVYNDINT-EWD-YQNLLK 508
Query: 572 DFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
+ K + S + EL+ L + K I+L EV +PV+D P+ L
Sbjct: 509 GYGAKNSRSYNIHSEKELLDLFKDEEFGKADV-IQLVEVHMPVLDAPRVL 557
>At5g54960 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 607
Score = 159 bits (402), Expect = 1e-38
Identities = 160/612 (26%), Positives = 266/612 (43%), Gaps = 59/612 (9%)
Query: 17 RSDSPVHGQSTVVKNIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQ 76
++ SP+H + + G Y+ RL+ G +F VPGDFNL L+
Sbjct: 30 QNTSPLHSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNL----TLLDHLIAEPNLK 85
Query: 77 WIGTCNELNGAYAADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSP 136
IG CNELN YAADGY+R + +G + T+ VG LS LN +AGA++E++ ++ I+G
Sbjct: 86 LIGCCNELNAGYAADGYAR-SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPN 144
Query: 137 SRFRENEQFRSHNVHHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQV 196
S N+ + +HH + D Q V C A ++++ A + +D
Sbjct: 145 S----NDYGTNRILHHTIGLPDFTQELR-------CFQAVTCFQAVINNLEEAHELIDTA 193
Query: 197 IVDICKNSKPGYVFIPVDFADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQW 256
I K SKP Y+ I + + ++P+ M + S ++ A + ++
Sbjct: 194 ISTALKESKPVYISISCNLP--AIPLPTFSRHPVPFMLPMKVS--NQIGLDAAVEAAAEF 249
Query: 257 IYESNAPSVFSDLLVDRFNLRKELRQFIETADVWNYNTAM---GKSTLDERNPKYLGLYK 313
+ ++ P + + + K F+E AD Y A+ K + E + ++G Y
Sbjct: 250 LNKAVKPVLVGG---PKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHFIGTYW 306
Query: 314 GAETGKEMQATVDSSDLILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKN 373
GA + V+S+D L GP N+ + YS + II + + I +
Sbjct: 307 GAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVT----IGNGP 362
Query: 374 A-STVEEANFVSVL--KYVNDNLDKSKLSFSYPPVPKSNHSCEYDNNDSITQKSLKEIIP 430
A V +F+S L + ++N Y P K + N+S+ L + I
Sbjct: 363 AFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRD---NPNESLRVNVLFQHIQ 419
Query: 431 NYFNDGDVLIVETGSFQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRS 490
N + ++ ETG F ++ Y Q Y SIG ++ A LG M +
Sbjct: 420 NMLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNR--- 476
Query: 491 HIVDQALVPKDYIPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAI-N 549
R+I C GDG+ Q+T Q+ ++ IR I + NN GYT+E I +
Sbjct: 477 --------------RVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHD 522
Query: 550 GPTRSYNDIAPFKWTALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAE 609
GP YN I + +TA + + E K + V +ELV+ +N + +E+ + E
Sbjct: 523 GP---YNVIKNWNYTAFVEAIHNGEGKC-WTAKVRCEEELVKAINT-ATNEEKESFCFIE 577
Query: 610 VMLPVMDIPQEL 621
V++ D +EL
Sbjct: 578 VIVHKDDTSKEL 589
>At5g01320 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 603
Score = 153 bits (387), Expect = 7e-37
Identities = 156/614 (25%), Positives = 270/614 (43%), Gaps = 60/614 (9%)
Query: 27 TVVKNIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNG 86
T G ++ RL+ +G +F VPGDFNL + N IG CNELN
Sbjct: 36 TTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNN----IGCCNELNA 91
Query: 87 AYAADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFR 146
YAADGY+R + +G + T+ VG LS LN +AGA++E++ V+ I+G S N+
Sbjct: 92 GYAADGYAR-SRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNS----NDFGT 146
Query: 147 SHNVHHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKP 206
+ +HH + D Q V C A ++++ A +++D+ I K SKP
Sbjct: 147 NRILHHTIGLPDFSQELR-------CFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKP 199
Query: 207 GYVFIPVDFADKLVSNKNLIQNPI-IDMDTVIESYKDENATKIAGDRLLQWIYESNAPSV 265
Y+ I + A + ++P+ D+ + + A A + L+++ ++ P +
Sbjct: 200 VYISISCNLA--ATPHPTFARDPVPFDLTPRMSNTMGLEA---AVEATLEFLNKAVKPVM 254
Query: 266 FSDLLVDRFNLRKELRQFIETADVWNYNTAMGKST---LDERNPKYLGLYKGAETGKEMQ 322
+ + K F+E AD Y A+ ST + E +P ++G Y GA +
Sbjct: 255 VGG---PKLRVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCS 311
Query: 323 ATVDSSDLILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANF 382
V+S+D + GP N+ + YS + II + ++ + + F
Sbjct: 312 EIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVV--VANGPTFGCVLMSDFF 369
Query: 383 VSVLKYVNDNLDKSKLSFSYPPVPKSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVE 442
+ K V N + + ++ VP+ + + + ++ + I + +I E
Sbjct: 370 RELAKRVKRN-ETAYENYERIFVPEGK-PLKCKPGEPLRVNAMFQHIQKMLSSETAVIAE 427
Query: 443 TGSFQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDY 502
TG F ++ + Y Q Y SIG ++ A LG QA K
Sbjct: 428 TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYA-------------QATPEK-- 472
Query: 503 IPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAI-NGPTRSYNDIAPF 561
R++ GDG+ Q+T Q+ ++ IR I + NN GYT+E I +GP YN I +
Sbjct: 473 --RVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEIHDGP---YNVIKNW 527
Query: 562 KWTALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
+T L++ + E K + V +ELV+ + K + +++ ++ EV++ D +EL
Sbjct: 528 NYTGLVDAIHNGEGKC-WTTKVRYEEELVEAI-KTATTEKKDSLCFIEVIVHKDDTSKEL 585
Query: 622 -----NAMLVNGKP 630
NG+P
Sbjct: 586 LEWGSRVSAANGRP 599
>At5g01330 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 592
Score = 152 bits (384), Expect = 1e-36
Identities = 157/606 (25%), Positives = 264/606 (42%), Gaps = 60/606 (9%)
Query: 35 GHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAADGYS 94
G ++ RL+ +G IF VPGDFNL + N IG CNELN YAADGY+
Sbjct: 33 GRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNN----IGCCNELNAGYAADGYA 88
Query: 95 RYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVHHLV 154
R + +G + T+ VG LS LN +AGA++E++ V+ I+G S N+ + +HH +
Sbjct: 89 R-SRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNS----NDFGTNRILHHTI 143
Query: 155 PAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYVFIPVD 214
D Q V C A ++ + A +++D+ I + SKP Y+ I +
Sbjct: 144 GLPDFSQELR-------CFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYISISCN 196
Query: 215 FADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDLLVDRF 274
A + + P+ T S KD + A + L+++ ++ P + +
Sbjct: 197 LA--AIPHPTFASYPVPFDLTPRLSNKD--CLEAAVEATLEFLNKAVKPVMVGG---PKL 249
Query: 275 NLRKELRQFIETADVWNYNTAM---GKSTLDERNPKYLGLYKGAETGKEMQATVDSSDLI 331
+ K F+E AD Y A+ K + E +P ++G Y GA + V+S+D
Sbjct: 250 RVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAY 309
Query: 332 LHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVL-KYVN 390
+ GP N+ + YS + II ++++ V + F L K V
Sbjct: 310 IFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVV---ANGPTFGCVRMSEFFRELAKRVK 366
Query: 391 DNLDKSKLSFSYPPVPKSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVETGSFQFGV 450
N K+ + + + + ++ + I ++ +I ETG F
Sbjct: 367 PN--KTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDSWFNC 424
Query: 451 PNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIPRLILCE 510
++ + Y Q Y SIG ++ A LG QA K R++
Sbjct: 425 QKLKLPKGCGYEFQMQYGSIGWSVGATLGYA-------------QATPEK----RVLSFI 467
Query: 511 GDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAI-NGPTRSYNDIAPFKWTALLNV 569
GDG+ Q+T Q+ ++ IR I + NN GYT+E I +GP YN I + +T L++
Sbjct: 468 GDGSFQVTAQDVSTMIRNGQKTIIFLINNGGYTIEVEIHDGP---YNVIKNWNYTGLVDA 524
Query: 570 FGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL-----NAM 624
+ E K + V +ELV+ +N + +++ ++ EV++ D +EL
Sbjct: 525 IHNGEGKC-WTTKVRYEEELVEAINT-ATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVS 582
Query: 625 LVNGKP 630
NG+P
Sbjct: 583 AANGRP 588
>At4g33070 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 607
Score = 150 bits (379), Expect = 6e-36
Identities = 143/593 (24%), Positives = 263/593 (44%), Gaps = 57/593 (9%)
Query: 35 GHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAADGYS 94
G ++ RL+ +G +F VPGDFNL L IG CNELN YAADGY+
Sbjct: 48 GRHLARRLVQAGVTDVFSVPGDFNL----TLLDHLMAEPDLNLIGCCNELNAGYAADGYA 103
Query: 95 RYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVHHLV 154
R + +G + T+ VG LS LN +AGA++E++ ++ I+G S N+ + +HH +
Sbjct: 104 R-SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNS----NDYGTNRILHHTI 158
Query: 155 PAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYVFIPVD 214
D Q V C A +++++ A +++D+ I K SKP Y+ + +
Sbjct: 159 GLPDFSQELR-------CFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCN 211
Query: 215 FADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDLLVDRF 274
A + + ++P+ ++ ++ + A + L+++ ++ P + +
Sbjct: 212 LA--AIPHHTFSRDPV--PFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGG---PKL 264
Query: 275 NLRKELRQFIETADVWNYNTAM---GKSTLDERNPKYLGLYKGAETGKEMQATVDSSDLI 331
+ K F+E AD Y AM K + E +P ++G Y GA + V+S+D
Sbjct: 265 RVAKACDAFVELADASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAY 324
Query: 332 LHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVLKYVND 391
+ GP N+ + YS + I ++Q I N T + ++
Sbjct: 325 IFAGPIFNDYSSVGYSLLLKKEKAI------VVQPDRITVANGPTFGCILMSDFFRELSK 378
Query: 392 NLDKSKLSFS--YPPVPKSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVETGSFQFG 449
+ +++ ++ + + ++ + + ++ + I + +I ETG F
Sbjct: 379 RVKRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFN 438
Query: 450 VPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIPRLILC 509
++ + Y Q Y SIG ++ A LG QA K R++
Sbjct: 439 CQKLKLPKGCGYEFQMQYGSIGWSVGATLGYA-------------QASPEK----RVLAF 481
Query: 510 EGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAI-NGPTRSYNDIAPFKWTALLN 568
GDG+ Q+T+Q+ ++ +R I + NN GYT+E I +GP YN I + +T L++
Sbjct: 482 IGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDGP---YNVIKNWNYTGLVD 538
Query: 569 VFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
+ E + V +ELV+ + + +++ + EV+L D +EL
Sbjct: 539 AIHNGEGNC-WTAKVRYEEELVEAITT-ATTEKKDCLCFIEVILHKDDTSKEL 589
>SPAC186.09 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 572
Score = 146 bits (368), Expect = 1e-34
Identities = 154/615 (25%), Positives = 264/615 (42%), Gaps = 82/615 (13%)
Query: 35 GHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAADGYS 94
GHY+ RL +G K F VPGD+NL N L+ + NELN A+AA+GY+
Sbjct: 17 GHYLAVRLAQAGVKHHFVVPGDYNLGLLDKLQYN----NYLEEVNCANELNCAFAAEGYA 72
Query: 95 RYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVHHLV 154
R CV+T Y VG +A +G+ GA+AED+ V+ I G SP+ N+ SH +HH +
Sbjct: 73 RANGIAACVVT-YSVGAFTAFDGIGGAYAEDLPVILISG-SPNT---NDIGSSHLLHHTL 127
Query: 155 PAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYVFIPVD 214
D ++M K ++ C++ + A +D I KP Y+ +P +
Sbjct: 128 GTHD------FSYQYEMAK-KITCAAVSIQRPTEAPRLIDYAIKMALLKKKPVYIEVPTN 180
Query: 215 FADKLVSNK---NLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDLLV 271
A + + +LI P ++ D +A + G + P + + +
Sbjct: 181 VASQPCAAPGPASLITEPETSNQEYLQMAVDISAKIVNGKQ---------KPVLLAGPKL 231
Query: 272 DRFNLRKELRQFIETADVWNYNTAM---GKSTLDERNPKYLGLYKGAETGKEMQATVDSS 328
F K F+E A+ N + A+ KS E +P Y G+Y G + ++ ++ S
Sbjct: 232 RSF---KAESAFLELANSLNCSVAVMPNAKSFFPESHPNYAGIYWGQASTLGAESIINWS 288
Query: 329 DLILHFGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVL-- 386
D I+ G + + ++ A ++ + D + TV +A F VL
Sbjct: 289 DCIICAGTTFTDYSSNGWT-SLPPKANVLHV-----------DVDRVTVSDAEFGGVLLR 336
Query: 387 KYVNDNLDKSKLS----FSYPPVPKSNHSCEYDN-NDSITQKSLKEIIPNYFNDGDVLIV 441
++++ K K + Y + + +N ++ +K + + N N L V
Sbjct: 337 DFLHELAKKVKANNASVVEYKRIRPESLEIPMENPKAALNRKEIIRQVQNLVNQETTLFV 396
Query: 442 ETGSFQFGVPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKD 501
+TG FG + + ++ + + IG ++P+A G G P+ ++V
Sbjct: 397 DTGDSWFGGMRITLPEKARFEIEMQWGHIGWSVPSAFGYAIGA---PKRNVV-------- 445
Query: 502 YIPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAI-NGPTRSYNDIAP 560
+ GDG+ Q T+QE + +R +P+ + + NN GYT+E I +GP YN I
Sbjct: 446 ------VFVGDGSFQETVQEVSQMVRLNLPIIMFLINNRGYTIEVEIHDGP---YNRIKN 496
Query: 561 FKWTALLNVFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQE 620
+ + A++ F E + V EL + + + K + L E + D +E
Sbjct: 497 WDYAAIVEAFNAGEG-HAKGFRVGNGHELAEAIRQAKENSQGPT--LIECNIDQDDCSKE 553
Query: 621 L-----NAMLVNGKP 630
L N NGKP
Sbjct: 554 LINWGHNVGAANGKP 568
>SPAC1F8.07c [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 594
Score = 143 bits (361), Expect = 7e-34
Identities = 150/593 (25%), Positives = 250/593 (41%), Gaps = 60/593 (10%)
Query: 35 GHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAADGYS 94
G Y+ +RL+ G K+ F VPGD+NL GL IG CNELN A+AA+GY+
Sbjct: 12 GTYLAQRLVEIGIKNHFVVPGDYNLRLLDFLEYYP----GLSEIGCCNELNCAFAAEGYA 67
Query: 95 RYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVHHLV 154
R +N + C + TY VG L+A +G+ GA+AE++ V+ + G SP+ N+ H +HH +
Sbjct: 68 R-SNGIACAVVTYSVGALTAFDGIGGAYAENLPVILVSG-SPNT---NDLSSGHLLHHTL 122
Query: 155 PAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYVFIPVD 214
D + Y + ++ C++ + A +D I KP Y+ IP +
Sbjct: 123 GTHDFE-------YQMEIAKKLTCAAVAIKRAEDAPVMIDHAIRQAILQHKPVYIEIPTN 175
Query: 215 FADKLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDLLVDRF 274
A++ + PI + + D+ + + A D + I + P + + +
Sbjct: 176 MANQPCP----VPGPISAV--ISPEISDKESLEKATDIAAELISKKEKPILLAGPKLRAA 229
Query: 275 NLRKELRQFIETADVWNYNTAMGKSTLDERNPKYLGLYKGAETGKEM-QATVDSSDLILH 333
+ E + + K E + Y G+Y G + E +A +SSDL++
Sbjct: 230 GAESAFVKLAEALNCAAFIMPAAKGFYSEEHKNYAGVYWGEVSSSETTKAVYESSDLVIG 289
Query: 334 FGPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFVSVL-KYVNDN 392
G N+ + + N N L N+ + I S V A F+ +L K V+
Sbjct: 290 AGVLFNDYSTVGWRAAPNPNI----LLNSDYTSVSIPGYVFSRVYMAEFLELLAKKVSKK 345
Query: 393 ---LDKSKLSFSYPPVPKSNHSCEYDNNDSITQKSLKEIIPNYFNDGDVLIVETGSFQFG 449
L+ + VPK+ + ++ + + I + L ETG F
Sbjct: 346 PATLEAYNKARPQTVVPKAA-----EPKAALNRVEVMRQIQGLVDSNTTLYAETGDSWFN 400
Query: 450 VPNMQFAREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIPRLILC 509
M+ K+ + + IG ++P+A+G + R I+
Sbjct: 401 GLQMKLPAGAKFEVEMQWGHIGWSVPSAMGYAVAAPER-----------------RTIVM 443
Query: 510 EGDGAAQMTIQEFASYIRYKIPMEILVWNNNGYTVERAI-NGPTRSYNDIAPFKWTALLN 568
GDG+ Q+T QE + IR+K+P+ I + NN GYT+E I +GP YN I + + A
Sbjct: 444 VGDGSFQLTGQEISQMIRHKLPVLIFLLNNRGYTIEIQIHDGP---YNRIQNWDFAAFCE 500
Query: 569 VFGDFENKFNESVTVEKNDELVQLLNKWKSCKERANIKLAEVMLPVMDIPQEL 621
K + + + +EL + KE L E + D QEL
Sbjct: 501 SLNGETGK-AKGLHAKTGEELTSAIKVALQNKEGPT--LIECAIDTDDCTQEL 550
>SPAC13A11.06 [EH] KOG1184 Thiamine pyrophosphate-requiring enzyme
Length = 152
Score = 117 bits (292), Expect = 7e-26
Identities = 68/163 (41%), Positives = 97/163 (58%), Gaps = 14/163 (8%)
Query: 31 NIPFGHYVFERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAA 90
+I G Y+F+RL G KSI GVPGDFNL +W+G NELNGAYAA
Sbjct: 4 DILVGEYLFKRLEQLGVKSILGVPGDFNLALLDLIEKVGD--EKFRWVGNTNELNGAYAA 61
Query: 91 DGYSRYTNKLGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNV 150
DGY+R N L ++TT+GVGELSA+NGVAG++AE V V+HI+G+ ++ ++ +
Sbjct: 62 DGYAR-VNGLSAIVTTFGVGELSAINGVAGSYAEHVPVVHIVGMPSTKVQDTGAL----L 116
Query: 151 HHLVPAIDSQQPPNHQVYFDMVKDRVCCSSAFLDDINTAADRL 193
HH + D + + DM K +V S +D+ N AA+++
Sbjct: 117 HHTLGDGD------FRTFMDMFK-KVSAYSIMIDNGNDAAEKI 152
>At3g48560 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 670
Score = 71.2 bits (173), Expect = 4e-12
Identities = 112/518 (21%), Positives = 204/518 (38%), Gaps = 75/518 (14%)
Query: 40 ERLLNSGAKSIFGVPGDFNLPXXXXXXXXXXVANGLQWIGTCNELNGAYAADGYSRYTNK 99
E L G +++F PG ++ + N L +E G +AA+GY+R + K
Sbjct: 105 EALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPR----HEQGGVFAAEGYARSSGK 160
Query: 100 LGCVITTYGVGELSALNGVAGAFAEDVKVLHIIGVSPSRFRENEQFRSHNVHHLVPAIDS 159
G I T G G + ++G+A A + V ++ I G P R + F+ + + +I
Sbjct: 161 PGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSI-- 218
Query: 160 QQPPNHQVYFDMVKD--RVCCSSAFLDDINTAADRLDQVIVDICKNSKPGYVFIPVDFAD 217
H V+D R+ + FL + R V+VD+ K+ + + A
Sbjct: 219 ---TKHNYLVMDVEDIPRIIEEAFFL----ATSGRPGPVLVDVPKDIQQQLAIPNWEQAM 271
Query: 218 KLVSNKNLIQNPIIDMDTVIESYKDENATKIAGDRLLQWIYESNAPSVFSDLLVDRFNLR 277
+L + + P D +++++ I ES P ++ + N
Sbjct: 272 RLPGYMSRMPKPPEDSHL---------------EQIVRLISESKKPVLY--VGGGCLNSS 314
Query: 278 KELRQFIETADVWNYNTAMGKSTL---DERNPKYLGLYKGAETGKEMQATVDSSDLILHF 334
EL +F+E + +T MG + DE + LG++ V+ SDL+L F
Sbjct: 315 DELGRFVELTGIPVASTLMGLGSYPCDDELSLHMLGMHGTVYA----NYAVEHSDLLLAF 370
Query: 335 GPAKNEINCGYYSFHFNENARIIELSNNIIQFIDIKDKNASTVEEANFV--SVLKYVNDN 392
G ++ G F A+I+ + + + K + S + + K + +
Sbjct: 371 GVRFDDRVTGKLE-AFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENR 429
Query: 393 LDKSKLSFSYPPVPKSNHSCEYDNNDSITQKSLKEIIPNYF--------NDGDVLIVETG 444
++ KL F N ++ K+ E IP + DG I+ TG
Sbjct: 430 AEELKLDFGV----WRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKA-IISTG 484
Query: 445 SFQFGVPNMQF---AREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKD 501
Q + QF + ++++ ++G LPAA+G P + +VD
Sbjct: 485 VGQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVA---NPDAIVVD------- 534
Query: 502 YIPRLILCEGDGAAQMTIQEFASYIRYKIPMEILVWNN 539
+GDG+ M +QE A+ +P+++L+ NN
Sbjct: 535 -------IDGDGSFIMNVQELATIRVENLPVKVLLLNN 565
>SPBP35G2.07 [EH] KOG4166 Thiamine pyrophosphate-requiring enzyme
Length = 669
Score = 65.9 bits (159), Expect = 2e-10
Identities = 120/564 (21%), Positives = 211/564 (37%), Gaps = 84/564 (14%)
Query: 11 LRTKSPRSDSPVHGQSTVVKNIPF-----GHYVFERLLNSGAKSIFGVPGDFNLPXXXXX 65
+R S +S S V S + + F G + +L K +FG PG LP
Sbjct: 61 VRANSTKSTSTVTTASPIKYDSSFVGKTGGEIFHDMMLKHNVKHVFGYPGGAILPVFDAI 120
Query: 66 XXXXXVANGLQWIGTCNELNGAYAADGYSRYTNKLGCVITTYGVGELSALNGVAGAFAED 125
+ ++I +E +AA YSR T K G V+ T G G + + +A A A+
Sbjct: 121 YR----SPHFEFILPRHEQAAGHAAQAYSRVTKKPGVVLVTSGPGATNVITPIADALADG 176
Query: 126 VKVLHIIGVSPSRFRENEQFRSHNVHHLVPAIDSQQPPNHQVYFDMVKDRVCCS--SAFL 183
++ G + ++ F+ DMV C+ + +
Sbjct: 177 TPLVVFSGQVATSAIGSDAFQE---------------------ADMVGISRSCTKWNVMV 215
Query: 184 DDINTAADRLDQVIVDICKNSKPGYVFI--PVDFADKLVSNKNLIQNPIIDMDTVIESYK 241
D+ R+D+ +I + +PG V + P D ++ I + + M+ ++
Sbjct: 216 KDVADLPRRIDEAF-EIATSGRPGPVLVDLPKDVTASVLKEPIPILSSVPSMNRRMKEVL 274
Query: 242 DENATKIAG--DRLLQWIYESNAPSVF-SDLLVDRFNLRKELRQFIETADVWNYNTAMGK 298
+E + + DR+ + + P +F ++ LR+F E + + +G
Sbjct: 275 EEGSKNVTAKIDRVANLLKLAKKPVIFCGHGVLANPECPTLLRKFSERLQIPVTTSLLGL 334
Query: 299 STLDERNP---KYLGLYKGAETGKEMQATVDSSDLILHFGPAKNEINCGYYSFHFNENAR 355
+DER+ LG++ MQ +DLIL G ++ G S F AR
Sbjct: 335 GAVDERSDLSLHMLGMHGSGYANMAMQ----EADLILALGVRFDDRVTGNVSL-FAPQAR 389
Query: 356 I--IELSNNIIQFIDIKDKNASTVE------EANFVSVLKYVNDNLDKSKLS-----FSY 402
+ E II F DI KN V E + LK ++ K+ S
Sbjct: 390 LAAAEERGGIIHF-DISPKNIGKVVQPTEAIEGDVYESLKLLDSATKNIKIPSRFDWLSQ 448
Query: 403 PPVPKSNHSCEYDNNDS----ITQKSLKEIIPNYFNDGDVLIVETG---SFQFGVPNMQF 455
K + + Q+ ++E+ + D + + TG + ++
Sbjct: 449 IQTWKERFPFTFTRSAPGELVKPQEVIQELDKQTSDIKDKVTITTGVGAHQMWAATFYRW 508
Query: 456 AREMKYITQSFYLSIGMALPAALGVGCGMRDYPRSHIVDQALVPKDYIPRLILCEGDGAA 515
+ +T ++G LPAA+G PKD + I +GD +
Sbjct: 509 TKPSSLVTSGGLGTMGFGLPAAIGA--------------SVAAPKDIV---IDIDGDASF 551
Query: 516 QMTIQEFASYIRYKIPMEILVWNN 539
MT E A+ ++ IP++IL+ NN
Sbjct: 552 SMTGMELATVRQFDIPVKILILNN 575
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,085,457
Number of Sequences: 60738
Number of extensions: 1738815
Number of successful extensions: 3985
Number of sequences better than 1.0e-05: 15
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3900
Number of HSP's gapped (non-prelim): 19
length of query: 631
length of database: 30,389,216
effective HSP length: 113
effective length of query: 518
effective length of database: 23,525,822
effective search space: 12186375796
effective search space used: 12186375796
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)