ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0769 suspect: LH U KOG2273 Intracellular trafficking, secretion, and vesicular transport Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0769 271880 273532 551
(551 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR132w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/... 479 e-135
SPAPJ696.01c [U] KOG2273 Membrane coat complex Retromer subunit ... 188 2e-47
>YOR132w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 551
Score = 479 bits (1234), Expect = e-135
Identities = 265/567 (46%), Positives = 368/567 (64%), Gaps = 34/567 (5%)
Query: 1 MTSSVXXXXXXXXXXXXFAEPVEPLAVPE-TINSITRGDQEQQPDTER-----------N 48
MTS+V FAEP E + P T + E++ TE+ N
Sbjct: 1 MTSAVPYDPYDDLDNNPFAEPQEEDSEPAATTTDGSSSMSEERVGTEQTAASVQDNGTAN 60
Query: 49 NKEHSSHKDNDSESTNTTNQQ-ENGNDSAVNDIPIDELLPERKNNK--YNLFVQVTGIER 105
N ++ ++ ++ + T+N+ EN S ++ I LPER + K Y L +VTG+ER
Sbjct: 61 NIQNGLGEEGNATRSKTSNEHNENQQPSQPSERVI---LPERSDEKKKYTLLAKVTGLER 117
Query: 106 AGSLTNKKENPSIKFDCTTNLPTFRXXXXXXXXXTYTEFYKLFKYLNAAIPETFVPSLPN 165
GS T KKENP+I FDC+TNLPTFR +Y EF++LFKYLN AI E+FVP+LP+
Sbjct: 118 FGSATGKKENPTIIFDCSTNLPTFRKQQYKNVKKSYEEFHQLFKYLNVAIQESFVPTLPS 177
Query: 166 SFTSYGINNEEDAIKTAANFQSWFNRITSDPLIIRSDEVAIFIESDFNTYTPLKKVSSI- 224
++T++GIN+EED +K NFQ WFNR++ DPLIIR++EVA FIESDFNTYTP+ K S+
Sbjct: 178 AYTTFGINSEEDRMKVTRNFQLWFNRLSQDPLIIRNEEVAFFIESDFNTYTPINKSKSLA 237
Query: 225 SGLKRKTLKQLSPPYDETLALAEFRPLVKSIYHTTQEIKAKLLKMATVRRQLSQEVNQFG 284
SGLKRKTLKQL+PPYDE LAEFRPLVKSIY +Q ++ KLL+++ R+ + QE N FG
Sbjct: 238 SGLKRKTLKQLAPPYDEITELAEFRPLVKSIYVVSQSLQEKLLRVSRNRKMMVQEENAFG 297
Query: 285 QAFQPLSLDNSDLYHKFGRVITAIGDIESIVANLDMATLFDPLESIEHDSYIIKEALTNR 344
Q F L +++ LY ++G+++TA+GDI+SI+A +DMATL+D LE I D+Y +KEALTNR
Sbjct: 298 QDFVNLD-EHNKLYRRYGKILTAVGDIDSIIATMDMATLYDGLEWIVRDAYAVKEALTNR 356
Query: 345 HFVMRELLQAQQTSRQRQENARKLRAKRDINPLKVDESIRQLKESTKNEHDLTIKLKRIT 404
HF+MR L+QAQQ S+ +QE AR+ R++RDINP+K+DE++RQLK + KNE LT+KL+RIT
Sbjct: 357 HFIMRNLVQAQQNSKAKQEQARRFRSRRDINPMKIDEALRQLKAAAKNEQVLTLKLQRIT 416
Query: 405 ANMLIERERWLVYLQETLAQAIKEFVLRKIEYDRKKLSLLERIRLTVRTADGKXXXXXXX 464
+NM+IER++W+ + +E + +IKEF LRKIEY+RKKL+LLER+R +R AD +
Sbjct: 417 SNMIIERKQWISWYEEWIRSSIKEFTLRKIEYERKKLTLLERVRSDIRKAD-ENGGLSRL 475
Query: 465 XXXXXXXXXXXXXXXXXXXXWTGDKTRTPHL-IGGIVTTEFDNAVFNPNNSHGETNTSQP 523
WTG+ R + I I TEFD+ +F ++ +
Sbjct: 476 GRHAVSNNNSDTSQTLKGDSWTGESNRKSQIPINKIAHTEFDDELFTEDDGY-------- 527
Query: 524 QSSRQSENIEQVGLSARQAASLLGDGT 550
S++ + L+AR AASLLG T
Sbjct: 528 ----NSQDSDTTSLNARHAASLLGMST 550
>SPAPJ696.01c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 549
Score = 188 bits (478), Expect = 2e-47
Identities = 127/461 (27%), Positives = 226/461 (48%), Gaps = 23/461 (4%)
Query: 96 LFVQVTGIERAGSLTNKKENPSIKFDCTTNLPTFRXXXXXXXXXTYTEFYKLFKYLNAAI 155
L +++ IE S ++P IK + T LP +R T+ EF K KYL +
Sbjct: 99 LRIRIVDIEAENS-----KDPVIKMNVQTTLPAYRSKLYKNVRRTHAEFKKFAKYLISTH 153
Query: 156 PETFVPSLPNSFTSYGINNEEDAIKTAANFQSWFNRITSDPLIIRSDEVAIFIESDFNTY 215
PE +P++P + TS +ED I + QSW N ++++P ++ E+ +F+ESD+ Y
Sbjct: 154 PECLIPAVPEAKTSVSSGIKEDLIYLKSGLQSWLNYVSTNPNLLYDPELQLFVESDYG-Y 212
Query: 216 TPLKKVSS-ISGLKRKTLKQLSPPYDETLALAEFRPLVKSIYHTTQEIKAKLLKMATVRR 274
+PL + SGLKRK LKQ P D ALA RP+VKS Y ++ + KL K+ ++
Sbjct: 213 SPLINTGNPTSGLKRKALKQFPLPPDPCQALANLRPIVKSFYKNAKDAEIKLEKLVNRKQ 272
Query: 275 QLSQEVNQFGQAFQPLSLDN-----SDLYHKFGRVITAIGDIESIVANLDMATLFDPLES 329
L+ GQ+ S++ ++ ++ G+++ AI D+ + ++ + TL D L
Sbjct: 273 SLALTHADLGQSLIDYSVEEQHNGLANALNRVGKMLQAISDVRIMQSSKQLVTLADSLCY 332
Query: 330 IEHDSYIIKEALTNRHFVMRELLQAQQTSRQRQENARKLRAKRDINPLKVDESIRQLKES 389
+++++KE L+NRH +MR+L+ ++ + A +L+ I+ + D++++ L+ +
Sbjct: 333 ASDNAFVVKEILSNRHILMRDLISSKNQTNSYLSAANRLQDSPKISKARTDDALQALEVA 392
Query: 390 TKNEHDLTIKLKRITANMLIERERWLVYLQETLAQAIKEFVLRKIEYDRKKLSLLERIRL 449
+E L+ K+ +T N++ E + + +L +AI+E+V ++ Y+R+ LS++E IR
Sbjct: 393 RVHEKLLSDKVDFVTLNLVKESKTYTKKTSVSLQKAIREYVEKEAYYERRLLSIMESIRP 452
Query: 450 TVRTADG-KXXXXXXXXXXXXXXXXXXXXXXXXXXXWTGDKTRTPHLIGGIVTTEFD--- 505
+R D WT K ++ FD
Sbjct: 453 HIRNIDPFGGLSRLGREEYPRRLSNPPPSQKTNQDAWTNRKRPG-------YSSSFDGSS 505
Query: 506 NAVFNPNNSHGETNTSQPQSSRQSENIEQVGLSARQAASLL 546
+ FNP+N+ G NTS+ I L + A+LL
Sbjct: 506 QSTFNPSNNDGAHNTSENADELVEPPIGNERLDPKSVANLL 546
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.130 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,944,587
Number of Sequences: 60738
Number of extensions: 1186942
Number of successful extensions: 5244
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5237
Number of HSP's gapped (non-prelim): 3
length of query: 551
length of database: 30,389,216
effective HSP length: 111
effective length of query: 440
effective length of database: 23,647,298
effective search space: 10404811120
effective search space used: 10404811120
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)