ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0785 good L KOG2379 Replication, recombination and repair Endonuclease MUS81
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0785 277093 278934 614
(614 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR386w [L] KOG2379 Endonuclease MUS81 427 e-119
SPCC4G3.05c [L] KOG2379 Endonuclease MUS81 224 2e-58
7290106 [L] KOG2379 Endonuclease MUS81 137 6e-32
Hs13376707 [L] KOG2379 Endonuclease MUS81 117 7e-26
At4g30870 [L] KOG2379 Endonuclease MUS81 108 3e-23
At5g39770 [L] KOG2379 Endonuclease MUS81 95 3e-19
ECU02g1390 [L] KOG2379 Endonuclease MUS81 79 3e-14
CE08680 [L] KOG2379 Endonuclease MUS81 77 1e-13
>YDR386w [L] KOG2379 Endonuclease MUS81
Length = 632
Score = 427 bits (1097), Expect = e-119
Identities = 246/636 (38%), Positives = 363/636 (56%), Gaps = 26/636 (4%)
Query: 1 MSIPSDAKQLYAEFLQQEVNQSTSAHQEKLVMVLNRALFALKNYPDPIYHPKDLLKVKGI 60
M + S+ K LY E+LQ+ V+ T QE+L + +A L+N Y+P DL KVKGI
Sbjct: 1 MELSSNLKDLYIEWLQELVDGLTPK-QEQLKIAYEKAKRNLQNAEGSFYYPTDLKKVKGI 59
Query: 61 GQTTMNKLSKRLKTYCEENGYSFPEEXXXXXXXXXXXXXXXPSNTQKTRVRTLEPEENES 120
G T + +L +L+ YC+ + S P E + ++ V + E ++NE+
Sbjct: 60 GNTIIKRLDTKLRNYCKIHHIS-PVEAPSLTQTSSTRPPKRTTTALRSIVNSCENDKNEA 118
Query: 121 ---QGTRKRRKKKYIPRNRSXXXXXXXXXXXXXCDKDGTACTRRQLIAVASKYCDQSYEK 177
+GT+KR+ +KYIP+ RS G + + Q+I VA KY D
Sbjct: 119 PEEKGTKKRKTRKYIPKKRSGGYAILLSLLELNAIPRGVS--KEQIIEVAGKYSDHCMTP 176
Query: 178 NPSTKEFYSAWSAIKSLKTNDLVIEQGRPSQFSLTEAGTILADSLKTANNIEFDVSSVY- 236
N STKEFY AWS+I +LK + LV+E+GRP ++SLTE G L SLKTA+ I F +
Sbjct: 177 NFSTKEFYGAWSSIAALKKHSLVLEEGRPKRYSLTEEGVELTKSLKTADGISFPKENEEP 236
Query: 237 -----------ERRLNRGNTQNSFTNDEHDHTVNFSGLM---NHANMSINESANSSRLFL 282
E N + + + +E +N + M ++S + ++
Sbjct: 237 NEYSVTRNESSEFTANLTDLRGEYGKEEEPCDINNTSFMLDITFQDLSTPQRLQNNVFKN 296
Query: 283 DATANSSRIEQNEEPEVSAEISTPIP----KQKVSKGRWKGVKYELWKPDSYDIILHIDH 338
D + + I ++ EVS + + P K + + R+ GV YELW +++ IDH
Sbjct: 297 DRLNSQTNISSHKLEEVSDDQTVPDSALKAKSTIKRRRYNGVSYELWCSGDFEVFPIIDH 356
Query: 339 REVRSKEDRGFFARKLLQRGIETESSSLTVGDMIWLAKHKQSGQQCALDFIVERKRLDDL 398
RE++S+ DR FF+R ++G+++E L +GD+IW+AK+K +G QC L+ IVERKRLDDL
Sbjct: 357 REIKSQSDREFFSRAFERKGMKSEIRQLALGDIIWVAKNKNTGLQCVLNTIVERKRLDDL 416
Query: 399 VISIRDNRFSEQKNRLQKTGCKHIFYLVEETTGYNVSDSADMMKTSIWTTVIYNDFHIKR 458
+SIRDNRF EQKNRL+K+GC+H +YL+EET N+ + + +KT++W ++Y F + R
Sbjct: 417 ALSIRDNRFMEQKNRLEKSGCEHKYYLIEETMSGNIGNMNEALKTALWVILVYYKFSMIR 476
Query: 459 TRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLTSLKMFRTEFERNKEIEC 518
T N+D TV+ + + +I YS+K L+VI + ++ Y L FR EFER IEC
Sbjct: 477 TCNSDETVEKIHALHTVISHHYSQKDLIVIFPSDLKSKDDYKKVLLQFRREFERKGGIEC 536
Query: 519 CHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQSRYPTFKTLLKAYRRCAA 578
CHN E Q M K +L TV EL + LM VKGISLEKA+ IQ +PT +L AY+ C++
Sbjct: 537 CHNLECFQELMGKGDLKTVGELTIHVLMLVKGISLEKAVAIQEIFPTLNKILMAYKTCSS 596
Query: 579 EADAKTLIQNELKDAPGNRKIGKSLSHTLWETFGKL 614
E +AK L+ N L DAPG +KI KSLS +++ FGKL
Sbjct: 597 EEEAKLLMFNVLGDAPGAKKITKSLSEKIYDAFGKL 632
>SPCC4G3.05c [L] KOG2379 Endonuclease MUS81
Length = 572
Score = 224 bits (572), Expect = 2e-58
Identities = 167/595 (28%), Positives = 283/595 (47%), Gaps = 51/595 (8%)
Query: 41 LKNYPDPIYHPKDLLKVKGIGQTTMNKLSKRLKTYCEENGYSFPEEXXXXXXXXXXXXXX 100
+K+ P + P L +KGIG T KL K+ YC EN
Sbjct: 1 MKSCPITFHRPSQALALKGIGPTICAKLEKKWNAYCLENNIPISTHNEQNDSHVNANKSS 60
Query: 101 XPSNTQKTRVRTLEPEENESQGTRKRRKKKYIPRNRSXXXXXXXXXXXXXCDKDGTACTR 160
++++K P + TRKR K Y+P RS + T+
Sbjct: 61 SETSSEK-------PRSVKKPTTRKR--KVYVPSYRSGAYSILCALYMLNKHE---FATK 108
Query: 161 RQLIAVASKYCDQSYEKNPSTKEFYSAWSAIKSLKTNDLVIEQGRPSQFSLTEAGTILAD 220
Q++ +A YCD S+ Y+AWSA+K+L T +LV + G PS++ LT+ G +
Sbjct: 109 PQIVTMAQPYCDSSFGSATDRNMRYTAWSAMKTLITKNLVYQTGHPSKYCLTDDGEEVCI 168
Query: 221 SLKTANNIEFDVSSVYERRLNRGNTQNSFTNDEHDHTV----NFSGLMNHANMSINESAN 276
L V ++R+ N S +D+HD ++ NF +N A S +
Sbjct: 169 RLAK-------VDDSFQRKHTVSNFSVS-KSDDHDSSLCQPPNFVTSINKAGSSSDHGGE 220
Query: 277 SSRLFLDATAN--SSRIEQNEEPEVSAEIS----------------TPIPKQKVSKGRWK 318
+ N S +E + + + ++ T K++ ++
Sbjct: 221 LHVTYCPVDHNEVSDGVETDIDVDQVDSLTGIHDHHIINNEQLIDLTEQEKKQPNESNLS 280
Query: 319 GVKYELWKPDSYDIILHIDHREVRSKEDRGFFARKLLQR-GIETESSSLTVGDMIWLAKH 377
+K E + + L ID RE+RS DR KL G+ + SL +GD +W+A+
Sbjct: 281 NLKIETVLFSNCTVFLLIDTREIRSPLDRNLIIDKLTNDFGVNCQVRSLELGDALWVARD 340
Query: 378 KQSGQQCALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEETTGYNVSDS 437
+SGQ+ LDF+VERKR DDLV SI+D RF EQK RL+K+G + + Y++EE++ Y+ S
Sbjct: 341 MESGQEVVLDFVVERKRYDDLVASIKDGRFHEQKARLKKSGIRSVTYILEESS-YDES-F 398
Query: 438 ADMMKTSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQS 497
+ ++T++ T + FH++ TR+ + +V L +M+ I Y ++ + + I + S
Sbjct: 399 TESIRTAVSNTQVDQLFHVRHTRSLEHSVSLLAEMTKQINLFYEKRKTLAV----IPDLS 454
Query: 498 IYLTSLKMFRTEFERNKEIECCH-NYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKA 556
I + + R + + H +Y + S + K++ +TV ++++R LM++KGIS KA
Sbjct: 455 IEAKTYESLREQLLKIDPSTPYHISYHAFSSVLSKSSTLTVGDIFIRMLMTIKGISASKA 514
Query: 557 LMIQSRYPTFKTLLKAYRRCAAEADAKTLIQNELKDAPGNRKIGKSLSHTLWETF 611
+ IQ +YPTF L +AY + ++ + + L+ N+ G + IG +LS + F
Sbjct: 515 IEIQKKYPTFMHLFEAYEK-SSSSQERNLLLNKTCQGYGFQTIGPALSAKVASVF 568
>7290106 [L] KOG2379 Endonuclease MUS81
Length = 426
Score = 137 bits (344), Expect = 6e-32
Identities = 88/289 (30%), Positives = 146/289 (50%), Gaps = 17/289 (5%)
Query: 326 KPDSYDIILHIDHREVRSKEDRGFFARK--LLQRGIETESSSLTVGDMIWLAKHKQSGQQ 383
KP + ++L +D +E K R + L G E LT+GD +W+A+ Q G +
Sbjct: 147 KPGQFQLLLLVDTQETSGKNKRVLDQTRSYLESLGARHEVRRLTIGDFLWVAQD-QEGNE 205
Query: 384 CALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEE-TTGYNVSDSADMMK 442
L +IVERKR+DDL SIRD RF EQK+RLQ G +HI YLVE+ + D ++
Sbjct: 206 LVLPYIVERKRMDDLASSIRDGRFHEQKHRLQHCGLQHIIYLVEDYGDNEQLGLPLDSLQ 265
Query: 443 TSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLTS 502
++ + + RT N ++ +L MS + ++++ K L ++ I + LT
Sbjct: 266 QALTNAKVQTGVRVVRTANHYRSMSYLAGMSRTLGQIFAGKKLHSVDRGAIDQSTCLLTH 325
Query: 503 LKMFRTEFERNKEIECCHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQSR 562
+ +F ++ K +TV+E++++ L+ + +SLE+A+ I R
Sbjct: 326 SDLGLLKF------------RALYEDSAKNAQLTVREVFVQQLLQLHSLSLERAMAIVER 373
Query: 563 YPTFKTLLKAYRRCAAEADAKTLIQNELKDAPGNRKIGKSLSHTLWETF 611
YPT + LL AY CA + A+ L+ + + P R +G +S L E +
Sbjct: 374 YPTPRWLLDAYAECADQKQARLLLAS-IPCGPLERPLGDKISQCLHEFY 421
>Hs13376707 [L] KOG2379 Endonuclease MUS81
Length = 476
Score = 117 bits (292), Expect = 7e-26
Identities = 85/292 (29%), Positives = 137/292 (46%), Gaps = 16/292 (5%)
Query: 326 KPDSYDIILHIDHREVRSKEDRGFFARKLLQRGIETESSSLTVGDMIWLAKHKQSGQ--- 382
+P Y ++L +D E R R R+L + + L VGD +W+A+
Sbjct: 188 RPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPAN 247
Query: 383 --QCALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEE-TTGYNVSDSAD 439
+ LD IVERKRLDDL SI D RF EQK RL++ G + YLVEE + +N+S
Sbjct: 248 PGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPES 307
Query: 440 MMKTSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIY 499
+ ++ T + + F +KRT + + +L ++ ++ LY H +
Sbjct: 308 TLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLY---------QGHTLRSRPW 358
Query: 500 LTSLKMFRTEFERNKEIECCHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMI 559
T + + + +K +V+E++ R LM V+G+S EKA +
Sbjct: 359 GTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAAL 418
Query: 560 QSRYPTFKTLLKAYRRCAAEADAKTLIQNELKDAPGNRKIGKSLSHTLWETF 611
RY T +LL AY CA + +TL+ + +K R +G +LS TL + +
Sbjct: 419 VDRYSTPASLLAAYDACATPKEQETLL-STIKCGRLQRNLGPALSRTLSQLY 469
>At4g30870 [L] KOG2379 Endonuclease MUS81
Length = 661
Score = 108 bits (269), Expect = 3e-23
Identities = 69/215 (32%), Positives = 107/215 (49%), Gaps = 25/215 (11%)
Query: 366 LTVGDMIWLAKHKQSGQQCALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYL 425
L VGD IW+A+HK + LDFI ERK +DD+ SIRDNR+ +QK RLQ++G K + Y+
Sbjct: 336 LPVGDCIWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYI 395
Query: 426 VEETTGYNVSDSADMMKTSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSL 485
+E N SD+A+ +KT+ +TT I F + RT T L + K +Y L
Sbjct: 396 LEGDP--NHSDAAESIKTACFTTEILEGFDVLRTHGLGET---LRKYGYLTKSIYQYYKL 450
Query: 486 VVINHDHITNQSIYLTSLKMFRTEFERNKEIECCHNYESMQSAMVKTNLMTVKELYLRAL 545
V ++D ++K C +++S + MT+ +++ L
Sbjct: 451 RVNDND--------------------QSKGAASCPSFDSFVKRCQDLDKMTISDVFAIQL 490
Query: 546 MSVKGISLEKALMIQSRYPTFKTLLKAYRRCAAEA 580
M V ++ E A+ + YPT +L AY E+
Sbjct: 491 MQVPQVTEEIAIAVLDMYPTLLSLASAYSHLYQES 525
>At5g39770 [L] KOG2379 Endonuclease MUS81
Length = 1242
Score = 95.1 bits (235), Expect = 3e-19
Identities = 108/421 (25%), Positives = 175/421 (40%), Gaps = 90/421 (21%)
Query: 180 STKEFYSAWSAIKSLKTNDLVIEQGRPSQFSLTEAGTILADSLKTANNIEFDVSSVYERR 239
S +E+YS WS + L LV++ P+++ LT G +AD L + + + + R
Sbjct: 867 SKREWYSGWSCMSKLIEKGLVVKFSNPAKYMLTVEGRNVADDLDPRSRGQSSIPAGPSSR 926
Query: 240 LNRGNTQNSFTNDEHDHTVNFSGLMNHANMSINESANSSRLFLDATANSSRIEQNEEPEV 299
Q D V+ G + A + S SS + A SS + +
Sbjct: 927 -----AQEFLATDTGQ--VDLEG--SRAKKFRSSSPCSSSISHALKACSSSLASD----- 972
Query: 300 SAEISTPIPKQKVSKGRWKGVKYELWKPDSYDIILHIDHREVRSKEDRGFFARKLLQRGI 359
E T IP+ + G +E + YD+IL +D RE FA K
Sbjct: 973 GTEGVTTIPRLPPLRF---GEAFE----EVYDVILILDDREQ--------FATK------ 1011
Query: 360 ETESSSLTVGDMIWLAKHKQSGQQCALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGC 419
HK + LDFIVERK +DD+ SI DNR+ +QK RLQ++G
Sbjct: 1012 ----------------GHKYQQDEYVLDFIVERKNVDDMRSSIMDNRYKDQKLRLQRSGI 1055
Query: 420 KHIFYLVEETTGYNVSDSADMMKTSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKEL 479
K + Y++E N S +A+ +KT+ +TT I F + RT T++ ++ I +
Sbjct: 1056 KKLIYILEGDP--NQSKTAESIKTACFTTEILEGFDVVRTNGLGETLRKYGYLTKSIYQY 1113
Query: 480 YSRKSLVVINHDHITNQSIYLTSLKMFRTEFERNKEIECCHNYESMQSAMVKTNLMTVKE 539
Y KSLV N D + K + C +Y+ MT+ +
Sbjct: 1114 Y--KSLV--NDD--------------------QRKVADSCPSYDDFVQRCPDLGKMTISD 1149
Query: 540 LYLRALMSVKGISLEK-----------ALMIQSRYPTFKTLLKAYRRCAAEADAKTLIQN 588
++ LM V +SL K A+ + +Y T ++L AY + + + +++N
Sbjct: 1150 VFAIQLMQV--LSLAKPSFGFLSATFFAIAVLDKYKTLRSLASAYSKLDNVSAQEEMLRN 1207
Query: 589 E 589
+
Sbjct: 1208 Q 1208
>ECU02g1390 [L] KOG2379 Endonuclease MUS81
Length = 487
Score = 78.6 bits (192), Expect = 3e-14
Identities = 61/241 (25%), Positives = 103/241 (42%), Gaps = 14/241 (5%)
Query: 331 DIILHIDHREVRSKEDRGFFARKLLQRGIETESSSLTVGDMIWLAKHKQSGQQCALDFIV 390
++ L ID RE++++ R FF R ++ E+ L GD +W+ + I+
Sbjct: 227 EVTLIIDAREIKNRRSRLFFQEYFELRQVKHETRVLEAGDFLWVRGERVCSS------II 280
Query: 391 ERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEETTGYNVSDSADMMKTSIWTTVI 450
ERKR D V SI D RF EQKNRL+ +G + IFY+VE ++ + S T
Sbjct: 281 ERKRGSDFVSSIMDGRFKEQKNRLRNSGIRRIFYVVEGLKNIHMQKVGKELVMSCLTATK 340
Query: 451 YNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLTSLKMFRTEF 510
F + TR+ T + + ++ +K+ + + + +L
Sbjct: 341 LEGFIVIETRDIVQTSSVIHMIDREVRGECEKKAKAMEGTEDHRERDGHL--------NI 392
Query: 511 ERNKEIECCHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQSRYPTFKTLL 570
N E +Y S K T+ L +L+S+KG+ +A + Y T L+
Sbjct: 393 SMNVGEETEMSYGSFIDRSSKGKGRTLTYLLYMSLLSIKGMGHRRAYALAEHYKTIGELI 452
Query: 571 K 571
+
Sbjct: 453 R 453
>CE08680 [L] KOG2379 Endonuclease MUS81
Length = 445
Score = 76.6 bits (187), Expect = 1e-13
Identities = 63/255 (24%), Positives = 115/255 (44%), Gaps = 39/255 (15%)
Query: 332 IILHIDHREVRSKEDRGFFARKLLQR-GIETESSSLTVGDMIWLAKHKQSGQQCALDFIV 390
+ L D+RE R+ L+++ I + SL+VGD IW+ + K G + +D++V
Sbjct: 209 VYLIADNREHRNNPRFKSVIEHLVKKEDIRVDIRSLSVGDYIWICR-KIDGTEIVMDWVV 267
Query: 391 ERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEETTGYNVSDSADMMKTSIWTTVI 450
ERK DDL SIR R+ EQK RL K+ YL+E +V+ + ++ +T+
Sbjct: 268 ERKTWDDLQSSIRGGRYDEQKGRLNMAPMKNRVYLIEAQHKGDVA-----CEQAVASTLS 322
Query: 451 YNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLTSLKMFRTEF 510
+ I+R + T +L ++++ ++ + + + +
Sbjct: 323 NGGYLIQRCSDTRDTAAFLKEVTIRLQNKAAIEEISGV---------------------- 360
Query: 511 ERNKEIECCHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQSRYPTFKTLL 570
+ +Q+ + K TVKE ++R LM G+S +A I R+P+ +LL
Sbjct: 361 ----------PFSQLQNLLQKKKAETVKEAWVRQLMVCPGMSQNRAEAIADRFPSMVSLL 410
Query: 571 KAYRRCAAEADAKTL 585
+R +A + L
Sbjct: 411 SYFRANGDDAPIRLL 425
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.130 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,273,493
Number of Sequences: 60738
Number of extensions: 1289719
Number of successful extensions: 3870
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3835
Number of HSP's gapped (non-prelim): 16
length of query: 614
length of database: 30,389,216
effective HSP length: 112
effective length of query: 502
effective length of database: 23,586,560
effective search space: 11840453120
effective search space used: 11840453120
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)