ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV0819    suspect: Pn   O     KOG3509  Posttranslational modification, protein turnover, chaperones   Basement membrane-specific heparan sulfate proteoglycan (HSPG) core protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV0819 289102 288818 -95 
         (95 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Hs7427517 [O] KOG3509 Basement membrane-specific heparan sulfate...    29   1.3  
Hs11415052 [T] KOG3575 FOG: Hormone receptors                          28   2.8  
At1g20760 [TU] KOG0998 Synaptic vesicle protein EHS-1 and relate...    27   3.7  
CE00501 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter                   27   6.3  
At3g20475 [L] KOG0221 Mismatch repair ATPase MSH5 (MutS family)        27   6.3  
At2g31100 [I] KOG4569 Predicted lipase                                 27   6.3  

>Hs7427517 [O] KOG3509 Basement membrane-specific heparan sulfate proteoglycan
            (HSPG) core protein
          Length = 4393

 Score = 28.9 bits (63), Expect = 1.3
 Identities = 13/47 (27%), Positives = 24/47 (50%)

Query: 13   VNPLTKIGLTSQLSHSIGTLAAEKIALTDLEISGPTPSPSMKETVYL 59
            V+P      + Q++ S GTL A  +   +    GP P+P + + +Y+
Sbjct: 2885 VSPADSGEYSCQVTGSSGTLEASVLVTIEPSSPGPIPAPGLAQPIYI 2931


>Hs11415052 [T] KOG3575 FOG: Hormone receptors
          Length = 533

 Score = 27.7 bits (60), Expect = 2.8
 Identities = 16/32 (50%), Positives = 18/32 (56%), Gaps = 2/32 (6%)

Query: 45  SGPTPSPSMKETVYLPVFGFGLP--VYLPILG 74
           SGP  SP +  TV LP  G G P  V  P+LG
Sbjct: 154 SGPVSSPQINSTVSLPGGGSGPPEDVKPPVLG 185


>At1g20760 [TU] KOG0998 Synaptic vesicle protein EHS-1 and related EH domain
           proteins
          Length = 1019

 Score = 27.3 bits (59), Expect = 3.7
 Identities = 19/53 (35%), Positives = 28/53 (51%), Gaps = 2/53 (3%)

Query: 17  TKIGLTSQLSHS-IGTLAAEKIALTDLEISG-PTPSPSMKETVYLPVFGFGLP 67
           +K  LT ++ ++ + T AA KI    + +S  P P P+   T   PV GFG P
Sbjct: 77  SKRDLTPEIVNAALNTPAAAKIPPPKINLSAIPAPRPNPAATTVGPVSGFGGP 129


>CE00501 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
          Length = 590

 Score = 26.6 bits (57), Expect = 6.3
 Identities = 22/70 (31%), Positives = 35/70 (49%), Gaps = 11/70 (15%)

Query: 9   SGIVVNPLTKIGLTSQLSHSI--GTLAAEKIALTDL-----EISGPTPSPSMKETVYLPV 61
           S  VVN +T +G+ S++SH +   T+ A   ++ DL      +    P  +M   +  P+
Sbjct: 447 SSEVVNVVTMLGVVSRVSHEVLGLTILAWSNSIGDLIADVSVVKQGYPRMAMAAAIGGPL 506

Query: 62  F----GFGLP 67
           F    GFGLP
Sbjct: 507 FNLLMGFGLP 516


>At3g20475 [L] KOG0221 Mismatch repair ATPase MSH5 (MutS family)
          Length = 321

 Score = 26.6 bits (57), Expect = 6.3
 Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 1/45 (2%)

Query: 34  AEKIALTDLEISGP-TPSPSMKETVYLPVFGFGLPVYLPILGCFL 77
           +EKI    + +  P T S +M+E V+L  +G  L   LP L  FL
Sbjct: 209 SEKIKFYTMSVLRPDTESANMEEIVFLYRYGVSLTSILPKLPTFL 253


>At2g31100 [I] KOG4569 Predicted lipase
          Length = 355

 Score = 26.6 bits (57), Expect = 6.3
 Identities = 13/41 (31%), Positives = 22/41 (52%)

Query: 27  HSIGTLAAEKIALTDLEISGPTPSPSMKETVYLPVFGFGLP 67
           HS+G + +   A   L    P  +PS++ ++ + VF FG P
Sbjct: 163 HSLGAVMSILSAADFLHNEWPKITPSLQHSLCVTVFAFGSP 203


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.322    0.140    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,413,882
Number of Sequences: 60738
Number of extensions: 202579
Number of successful extensions: 448
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 446
Number of HSP's gapped (non-prelim): 6
length of query: 95
length of database: 30,389,216
effective HSP length: 71
effective length of query: 24
effective length of database: 26,076,818
effective search space: 625843632
effective search space used: 625843632
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)