ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0831 good R KOG1432 General function prediction only Predicted DNA repair exonuclease SIA1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0831 292358 290625 -578
(578 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR137c [R] KOG1432 Predicted DNA repair exonuclease SIA1 240 5e-63
YLR361c [R] KOG1432 Predicted DNA repair exonuclease SIA1 103 9e-22
At5g63140 [R] KOG1432 Predicted DNA repair exonuclease SIA1 85 3e-16
At5g57140 [R] KOG1432 Predicted DNA repair exonuclease SIA1 83 1e-15
SPCC1020.05 [R] KOG1432 Predicted DNA repair exonuclease SIA1 73 1e-12
At2g46880 [R] KOG1432 Predicted DNA repair exonuclease SIA1 70 9e-12
>YOR137c [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 622
Score = 240 bits (612), Expect = 5e-63
Identities = 194/652 (29%), Positives = 307/652 (46%), Gaps = 125/652 (19%)
Query: 5 RRILLYARRFFLVWICFLFITSWSLIQRHLKNWLVQAPDLKLSGSLQDTGIKVRDVRISK 64
RR R F++ I L+++ SL + H KN L+ + G + D D++I +
Sbjct: 7 RRFNFLRRILFILCITSLYLSRDSL-KLHAKNVLMDHNVAEYHGGMID------DIQILR 59
Query: 65 CYSWFSNCDAIWDYSLQD----------DLVTWYRIDMGKMQDT--SIGQGFGWTSYVYW 112
CY W+ C +++ L + + W R+ +T S+ G + SY+Y
Sbjct: 60 CYHWYRQCSSLYAPKLHPSNTAKKIKDKNSILWTRVSKNITVETLYSLQSGPFYNSYLYV 119
Query: 113 QPL-YPNERGSAVVDLALSRNG--LPLGLANERYNKIKNNDIDGYHVHSNTDNEFYVKDD 169
+ + + + +LA++R+ +PL + + +K++D +H N Y+++
Sbjct: 120 HLKDFQSNPKNTIKELAIARDSALIPLQVLRDINKLVKSSDSSVFH------NHVYLREK 173
Query: 170 SSNRFNPDMKWSAKFGNHL---------EDWFWRGDGIWCKYGRRSNG----IKEIKAFI 216
++ + W FG + E+W ++G GIWCKY + I ++ ++
Sbjct: 174 PTSSW-----WKLLFGISVDTDNIAVFGEEWVYKGSGIWCKYILNDDDNDAPITNLEIYL 228
Query: 217 GEDFIESRPMWKEMVHCLHREGY-SKPISISFQ---KSRLDDFTYG-------KEKDLS- 264
G FIESRP WKE++H HR S PISI+ + K+ F+ G KD++
Sbjct: 229 GSSFIESRPSWKEVIHEFHRNNIPSLPISITRKLETKNHHHKFSNGLLGSLRTPSKDINI 288
Query: 265 ------QISEPSLVMKRAD--FKILQISDLHFGRHIVSDSRKEKPDSIFRYDWPNVQFIH 316
+I+ P + R FKILQI+D HF +D+ + I V FI
Sbjct: 289 QVDADYKITSPHIQFSRGQRSFKILQITDFHFK---CTDNSMTVINEI-----KTVNFID 340
Query: 317 SVIRNERPDLAVITGHIFKDFNKNLDYESQILKMVSPIISNGIPFLFTWG---EPQVTTE 373
V+ +E PDL VITG + D + +DY++ I+K+V P+ISN IP+ + G E + T
Sbjct: 341 RVLASENPDLVVITGDLL-DSHNTIDYQTCIMKVVQPMISNKIPYAISLGVSDESNLATS 399
Query: 374 FKVNILNFIKSLPFCLN---------------KFDLKNSTYLMLPLLLPAKTPGSQKQIG 418
++ +FI++LP+ N K L +T L + +T S+
Sbjct: 400 AQIR--DFIRNLPYTFNNVASEEGHMAIEVSFKKKLTKNTLLERDIDTEDETNPSE---A 454
Query: 419 TIFAFDSNVTESYNFLDKFPRSPQSV-YNLAFQHLPLHEYRPQGSFALIGNYEQKGSLDY 477
F FDS + NFL + + + LAFQ+ PL EYRP G F +IG Y ++ +L
Sbjct: 455 LFFVFDSFAPVN-NFLQDYNDLIGKIDFGLAFQYFPLSEYRPHGLFPIIGQYNERSTLTV 513
Query: 478 IP--------------HTKAFRNLLGEKDIKAISCGHEHGNDCCVLSDGKQQNLKNNMWL 523
H K+F ++L +IK +SCGHEH NDCC+ S KN MWL
Sbjct: 514 DTPRSRGQVSMTINGKHYKSFLDILSLWNIKGVSCGHEHNNDCCLQS-------KNEMWL 566
Query: 524 CYGGVTGYD----QAYESKVRIFKIDTEKNDITSWKRSIKDTSKVSDYQYIW 571
CYGG G Q VR+F +D ++ITSWKR+ +V DYQYI+
Sbjct: 567 CYGGSAGIGLPRIQGIYPTVRLFNLDDILDEITSWKRNSNLVDEVYDYQYIY 618
>YLR361c [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 578
Score = 103 bits (256), Expect = 9e-22
Identities = 125/504 (24%), Positives = 203/504 (39%), Gaps = 95/504 (18%)
Query: 104 FGWTSYVYWQPLYPNERGSAVVDLALSRNGLPLGLANERYNKIKNNDIDGYHVHSNTDNE 163
FG + Y+Y+ LYP A+VD ++++ +V + T
Sbjct: 120 FGKSEYLYYDVLYP-----ALVDYF-------------------GSNLEKLNVEAVTGIS 155
Query: 164 FYVKDDSSNRFNPDMKWSAKFGNHLEDWFWRGDGIWCKYGRRSNGIKEIKAFIGEDFIES 223
Y KD S P M S F + I + ++ + I +I G D I+
Sbjct: 156 KYPKDKSL----PFMDVSITF-----------EPISIELLQKRSYISDINILFGVDCIQP 200
Query: 224 RPMW---KEMVHCLHREGYSKPISISFQKSRLDDFTYGKEKDLSQISEPSLVMKRADFKI 280
W KE +R YS+P ++++ G ++ L + E FKI
Sbjct: 201 IANWTLQKEFPLVKYR--YSEPAYLTYKFVGTRPVDTGAQR-LQETDE-------GKFKI 250
Query: 281 LQISDLHFGRHIVSDSRKEKPDSIFRYDWPNVQFIHSVIRNERPDLAVITGHIFKDFNKN 340
+Q++DLH G + + D F+ V+ E+P L V TG
Sbjct: 251 VQLADLHLGVGESECIDEYPKHEACKADPKTETFVQQVLDIEKPQLVVFTGDQIMGDRSI 310
Query: 341 LDYESQILKMVSPIISNGIPFLFTWGEPQVTTEFKVNILNFIKS-LPFCLNKF---DLKN 396
D E+ +LK V+P+I+ IP+ WG L+ I S LP+ L KF D +
Sbjct: 311 QDSETVLLKAVAPVIARKIPWAMVWGNHDDEGSLTRWQLSEIASVLPYSLFKFSPHDTHD 370
Query: 397 STYLMLPLLLPA-KTPGSQKQIGTIFAFDSN----VTESYNFLDKFPRSP---------- 441
+T+ + + ++ +GT++ DS+ V + Y D S
Sbjct: 371 NTFGVGNYIYQIFSNNDTEVPVGTLYFLDSHKYSTVGKIYPGYDWIKESQWKYIEDYHDV 430
Query: 442 ----QSVYNLAFQHLPLHEY-------RPQGSFALIGNYEQKGSLDYIPHTKAFRNLLGE 490
++ ++AF H+PL EY P LIG Y K + + L
Sbjct: 431 NLKFKTGLSMAFFHIPLPEYLNIESKTHPGEKNPLIGMY--KEGVTAPKYNSEGITTLDR 488
Query: 491 KDIKAISCGHEHGNDCCVLSDGKQQNLKNNMWLCY------GGVTGYDQAYESKVRIFKI 544
+ +SCGH+H ND C+ D + N +WLCY GG GY E ++RI++I
Sbjct: 489 LSVDVVSCGHDHCNDYCLRDD----STPNKIWLCYGGGGGEGGYAGYG-GTERRIRIYEI 543
Query: 545 DTEKNDITSWKRSIKDTSKVSDYQ 568
+ +N+I +WKR ++ D+Q
Sbjct: 544 NVNENNIHTWKRLNGSPKEIFDFQ 567
>At5g63140 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 389
Score = 84.7 bits (208), Expect = 3e-16
Identities = 86/348 (24%), Positives = 143/348 (40%), Gaps = 57/348 (16%)
Query: 277 DFKILQISDLHFGRHIVSDSRKEKPDSIFRY-DWPNVQFIHSVIRNERPDLAVITGHIFK 335
+FKILQ++D+HF + + P D F+ VI E+PDL V TG
Sbjct: 46 EFKILQVADMHFANGAKTQCQNVLPSQRAHCSDLNTTIFMSRVIAAEKPDLIVFTGDNIF 105
Query: 336 DFNKNLDYESQILKMVSPIISNGIPFLFTWGE-PQVTTEFKVNILNFIKSLPFCLNKFDL 394
F+ D I +P I++ IP++ G Q +T + ++N I LP L++ +
Sbjct: 106 GFDVK-DALKSINAAFAPAIASKIPWVAILGNHDQESTFTRQQVMNHIVKLPNTLSQVNP 164
Query: 395 KNSTYLM-------LPLLLPAKTPGSQKQIGTIFAFDSN------VTESYNFL------- 434
+ + + L + A + K + ++ DS E Y+++
Sbjct: 165 PEAAHYIDGFGNYNLQIHGAADSKLQNKSVLNLYFLDSGDYSSVPYMEGYDWIKTSQQFW 224
Query: 435 -DKFPRSPQSVYN------------LAFQHLPLHEYRPQGSFALIGNYEQKGSLDYIPHT 481
D+ + + YN LA+ H+PL E+ S Q+G+ ++
Sbjct: 225 FDRTSKRLKREYNAKPNPQEGIAPGLAYFHIPLPEFLSFDSKNATKGVRQEGTSAASTNS 284
Query: 482 KAFRNLLGEKDIKAISCGHEHGNDCCVLSDGKQQNLKNNMWLCYGGVTGY----DQAYES 537
F L+ D+K++ GH+H ND C G+ + L LCYGG GY +E
Sbjct: 285 GFFTTLIARGDVKSVFVGHDHVNDFC----GELKGLN----LCYGGGFGYHAYGKAGWER 336
Query: 538 KVRIFKIDTEK---------NDITSWKRSIKDTSKVSDYQYIWSRTLN 576
+ R+ +D K I +WKR V D Q +W+ + N
Sbjct: 337 RARVVVVDLNKKRKGKWGAVKSIKTWKRLDDKHLSVIDSQVLWNNSAN 384
>At5g57140 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 397
Score = 82.8 bits (203), Expect = 1e-15
Identities = 89/356 (25%), Positives = 149/356 (41%), Gaps = 65/356 (18%)
Query: 269 PSLVMKRAD---FKILQISDLHFGRHIVSDSRKEKPDSIFRY--DWPNVQFIHSVIRNER 323
P+L ++ D FKILQ++D+HFG +++ R + DS F Y D +F+ +I +ER
Sbjct: 46 PNLPLRFRDDGTFKILQVADMHFGMGMITRCR-DVLDSEFEYCSDLNTTRFLRRMIESER 104
Query: 324 PDLAVITG-HIFKDFNKNLDYESQILKMVSPIISNGIPFLFTWG-EPQVTTEFKVNILNF 381
PDL TG +IF + D +L+ + P I GIP+ G +T ++ ++ F
Sbjct: 105 PDLIAFTGDNIFG--SSTTDAAESLLEAIGPAIEYGIPWAAVLGNHDHESTLNRLELMTF 162
Query: 382 IKSLPFCLNKF------DLKNSTYLMLP-----LLLPAKTPGS---QKQIGTIFAFDSNV 427
+ + F +++ + K T ++ + PGS + +F FDS
Sbjct: 163 LSLMDFSVSQINPLVEDETKGDTMRLIDGFGNYRVRVYGAPGSVLANSTVFDLFFFDSGD 222
Query: 428 TE------SYNFLDK----------FPRSPQSVY----NLAFQHLPLHEYRPQGSFALIG 467
E +Y ++ + Q ++ LAF H+P+ E R IG
Sbjct: 223 REIVQGKRTYGWIKESQLRWLQDTSIQGHSQRIHVNPPALAFFHIPILEVRELWYTPFIG 282
Query: 468 NYEQKGSLDYIPHTKAFRNLLGEKDIKAISCGHEHGNDCCVLSDGKQQNLKNNMWLCYGG 527
+ Q+G I + + + ++KA GH+H ND C G +W CYGG
Sbjct: 283 QF-QEGVACSIVQSGVLQTFVSMGNVKAAFMGHDHVNDFCGTLKG--------VWFCYGG 333
Query: 528 VTGYD----QAYESKVRIFKIDTEKND--------ITSWKRSIKDTSKVSDYQYIW 571
GY + + R+ + K I +WKR + D Q +W
Sbjct: 334 GFGYHAYGRPNWHRRARVIEAKLGKGRDTWEGIKLIKTWKRLDDEYLSKIDEQVLW 389
>SPCC1020.05 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 509
Score = 73.2 bits (178), Expect = 1e-12
Identities = 85/380 (22%), Positives = 152/380 (39%), Gaps = 36/380 (9%)
Query: 197 GIWCKYGRRSNGIKEIKAFIGEDFIESRPMWKEMVHCLHREGYSKPISISFQKSRLD--- 253
GI +YG + I E +GED +E R W +++ G +P+ ++ +
Sbjct: 127 GIQIRYGTDGD-ICEFNTLLGEDVVELREGWSAVLNDFIYLG--QPVLLTQRPCETPPTP 183
Query: 254 DFTYGKEKDLSQISEPSLVMKRADFKILQISDLHFGR--HIVSDSRKEKPDSIFRYDWPN 311
K K+LS ++ ++ KILQ+SDLH+ D + D
Sbjct: 184 SIEALKRKELSSVTLTYDDEEKKTIKILQLSDLHYSNSDRPCRDPYPYETAEDCMADAKT 243
Query: 312 VQFIHSVIRNERPDLAVITGHIFKDFNKNLDYESQILKMVSPIISNGIPFLFTWGE---- 367
F++ +++ E PD ++TG + + + D S ++K VSP + +PF +G
Sbjct: 244 TAFVNELLQLEEPDFVLLTGDLING-DTSRDARSSLMKAVSPFVDYNVPFAVNFGNHDDL 302
Query: 368 PQVTTEFKVNILNFIKSLPFCLNKFDLKNSTYLMLP---LLLPAKTPGSQKQIGTIFAFD 424
++ E IL+ I + + L P + T G +D
Sbjct: 303 GDLSREELAKILSQIPGSMGLIGNVSGVGNFVLHSPRKFAIYVLDTKGDTSNRRLCPGYD 362
Query: 425 SNVTESYNFL-DKFPRSPQSVYNLAFQHLPLHEYRPQGSFALIGNYEQKGSLDYI-PHT- 481
+ + +L K +A H+PL E+ L+G + + S P+T
Sbjct: 363 AITEDQLEWLSSKVADFKYEPIQMAVLHIPLKEFCETED--LVGAFREPCSYSICDPNTA 420
Query: 482 KAFRNLLGEKDIKAISCGHEHGNDCCVLSDGKQQNLKNNMWLCYGGVTGY-----DQAYE 536
KA ++L I GH+H ND C + + N + C+ G G+ Y
Sbjct: 421 KALKSL----RIPLAIAGHDHVNDFCGI------HPDYNTYFCFAGGAGFGGYGGHGGYV 470
Query: 537 SKVRIFKIDTEKNDITSWKR 556
+ R+F++D + + +WKR
Sbjct: 471 RRARVFELDPVERAVRTWKR 490
>At2g46880 [R] KOG1432 Predicted DNA repair exonuclease SIA1
Length = 437
Score = 70.1 bits (170), Expect = 9e-12
Identities = 77/349 (22%), Positives = 131/349 (37%), Gaps = 64/349 (18%)
Query: 278 FKILQISDLHFGRHIVSDSRKEKPDSI-FRYDWPNVQFIHSVIRNERPDLAVITGHIFKD 336
FKILQ+SD+H+G + P + D F+ I +E+PDL V +G
Sbjct: 46 FKILQVSDMHYGFGKETQCSDVSPAEFPYCSDLNTTSFLQRTIASEKPDLIVFSGDNVYG 105
Query: 337 FNKNLDYESQILKMVSPIISNGIPFLFTWG-EPQVTTEFKVNILNFIKSLPFCLNK---- 391
+ D + +P I +GIP++ G Q + + ++ +I LP L++
Sbjct: 106 LCETSDVAKSMDMAFAPAIESGIPWVAILGNHDQESDMTRETMMKYIMKLPNSLSQVNPP 165
Query: 392 ----FDLKNSTYLMLPLLLPAKTPGSQKQIGTIFAFDSNVTESYNFLDKF-------PRS 440
+ + L + P +P K I ++ D SY LD F S
Sbjct: 166 DAWLYQIDGFGNYNLQIEGPFGSPLFFKSILNLYLLDGG---SYTKLDGFGYKYDWVKTS 222
Query: 441 PQSVY-----------------------NLAFQHLPLHEYRPQGSFALIGNYEQKGSLDY 477
Q+ Y L + H+P+ E+ + Q+ +
Sbjct: 223 QQNWYEHTSKWLEMEHKRWPFPQNSTAPGLVYLHIPMPEFALFNKSTEMTGVRQESTCSP 282
Query: 478 IPHTKAFRNLLGEKDIKAISCGHEHGNDCCVLSDGKQQNLKNNMWLCYGGVTGY----DQ 533
++ F L+ ++K + GH+H ND C G + LCY G GY
Sbjct: 283 PINSGFFTKLVERGEVKGVFSGHDHVNDFCAELHG--------INLCYAGGAGYHGYGQV 334
Query: 534 AYESKVRIFKIDTEK---------NDITSWKRSIKDTSKVSDYQYIWSR 573
+ +VR+ + EK + I +WKR + D Q +W++
Sbjct: 335 GWARRVRVVEAQLEKTMYGRWGAVDTIKTWKRLDDKNHSLIDTQLLWTK 383
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.139 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,019,410
Number of Sequences: 60738
Number of extensions: 1765272
Number of successful extensions: 3522
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 3494
Number of HSP's gapped (non-prelim): 10
length of query: 578
length of database: 30,389,216
effective HSP length: 112
effective length of query: 466
effective length of database: 23,586,560
effective search space: 10991336960
effective search space used: 10991336960
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)