ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0868 good H KOG3153 Coenzyme transport and metabolism Thiamine pyrophosphokinase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0868 304939 304028 -304
(304 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR143c [H] KOG3153 Thiamine pyrophosphokinase 228 1e-59
At2g44750 [H] KOG3153 Thiamine pyrophosphokinase 93 4e-19
Hs21362110 [H] KOG3153 Thiamine pyrophosphokinase 85 1e-16
SPAC6F12.05c_2 [H] KOG3153 Thiamine pyrophosphokinase 79 8e-15
At1g02880 [H] KOG3153 Thiamine pyrophosphokinase 75 2e-13
7299502 [H] KOG3153 Thiamine pyrophosphokinase 58 2e-08
>YOR143c [H] KOG3153 Thiamine pyrophosphokinase
Length = 319
Score = 228 bits (580), Expect = 1e-59
Identities = 126/286 (44%), Positives = 175/286 (61%), Gaps = 23/286 (8%)
Query: 34 ENSVLLILNQRIKLDHSAFEALWNSYTIKICADGAANRLFDYYRDSDGKR--YHPDVIAG 91
ENS LLILNQ+I + F +W + +K+CADGAANRL+DY D + R Y P+ I G
Sbjct: 37 ENSTLLILNQKIDIPRPLFYKIWKLHDLKVCADGAANRLYDYLDDDETLRIKYLPNYIIG 96
Query: 92 DMDSIREDVLEYYEAIETTTVIKQNTQYSTDFTKSVNVATLLLLGIDLNAVEINEYD--- 148
D+DS+ E V +YY T+IKQ TQYSTDFTK VN+ +L + ++ N+ +
Sbjct: 97 DLDSLSEKVYKYYRK-NKVTIIKQTTQYSTDFTKCVNLISLHFNSPEFRSLISNKDNLQS 155
Query: 149 --------GIHKLYQQADKS----QLKDIPLLILNGIDGRFDHTIHSMVQFYALRRQDPY 196
GIH LY +S ++ I LL L GI GRFD T+HS+ Q Y L Y
Sbjct: 156 NHGIELEKGIHTLYNTMTESLVFSKVTPISLLALGGIGGRFDQTVHSITQLYTLSENASY 215
Query: 197 FKMCFLTMSDMIILIPSSKKGYWLKLDKV--KPLIGNCGLLPLAGPNIITKTKGLKWDVE 254
FK+C++T +D+I LI K G ++ D IGNCGLLP+ ++ +T+GLKWDV+
Sbjct: 216 FKLCYMTPTDLIFLI--KKNGTLIEYDPQFRNTCIGNCGLLPIGEATLVKETRGLKWDVK 273
Query: 255 DWPTSIESGNVSSSNRFVGEN-CLIACEESIVMSVELKWDKITQHL 299
+WPTS+ +G VSSSNRFVG+N C I ++ I+++VE+ DK+ L
Sbjct: 274 NWPTSVVTGRVSSSNRFVGDNCCFIDTKDDIILNVEIFVDKLIDFL 319
>At2g44750 [H] KOG3153 Thiamine pyrophosphokinase
Length = 263
Score = 93.2 bits (230), Expect = 4e-19
Identities = 73/264 (27%), Positives = 126/264 (47%), Gaps = 45/264 (17%)
Query: 38 LLILNQRIKLDHSAFEALWNSYTIKICADGAANRLFD----YYRDSDG----KRYHPDVI 89
L++LNQ + LW +++CADG ANR++D ++ D RY PDVI
Sbjct: 24 LVVLNQNLP---RFTPLLWEHAKLRLCADGGANRIYDELPLFFPHEDPFVIRNRYKPDVI 80
Query: 90 AGDMDSIREDVLEYYEAIETTTVIKQNTQYSTDFTKSVNVATLLLLGIDLNAVEINEYDG 149
GDMDSIR DVL++Y T + + + Q +TD K ++ Y
Sbjct: 81 KGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDLDKCIS------------------YIR 122
Query: 150 IHKLYQQADKSQLKDIPLLILNGIDGRFDHTIHSMVQFYALRRQDPYFKMCFLTMSDMII 209
L Q++ + Q +L + GRFDH ++ Y + P ++ L+ +I
Sbjct: 123 HSTLNQESSRLQ-----ILATGALGGRFDHEAGNLNVLY----RYPDTRIVLLSDDCLIQ 173
Query: 210 LIPSSKKGYWLKLDKVKPLIG-NCGLLPLAGPNIITKTKGLKWDVEDWPTSIESGNVSSS 268
L+P + + ++ L G +CGL+P+ P+ T T GLKWD+ + T + G + S+
Sbjct: 174 LLPKTHRH---EIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSN--TEMRFGGLIST 228
Query: 269 NRFVGENCL-IACEESIVMSVELK 291
+ V E + + + ++ ++ +K
Sbjct: 229 SNLVKEEIITVESDSDLLWTISIK 252
>Hs21362110 [H] KOG3153 Thiamine pyrophosphokinase
Length = 243
Score = 85.1 bits (209), Expect = 1e-16
Identities = 70/237 (29%), Positives = 113/237 (47%), Gaps = 35/237 (14%)
Query: 38 LLILNQRIKLDHSAFEALWNSYTIKICADGAANRLFDYYRDSDGKRYHPDVIAGDMDSIR 97
L+ILNQ + + F LWN ++ CADG ANRL+D + + + + P+ I GD DSIR
Sbjct: 21 LVILNQPLD---NYFRHLWNKALLRACADGGANRLYD-ITEGERESFLPEFINGDFDSIR 76
Query: 98 EDVLEYYEAIETTTVIKQNTQYSTDFTKSVNVATLLLLGIDLNAVEINEYDGIHKLYQQA 157
+V EYY A + +I Q TDFTK + + L ++
Sbjct: 77 PEVREYY-ATKGCELISTPDQDHTDFTKCLKM-----------------------LQKKI 112
Query: 158 DKSQLKDIPLLILNGIDGRFDHTIHSMVQFYALRRQDPYFKMCFLTMSDMIILIPSSKKG 217
++ LK ++ L G+ GRFD + S+ + P F + + +I L+ K
Sbjct: 113 EEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITP-FPIIIIQEESLIYLLQPGKH- 170
Query: 218 YWLKLDKVKPLIGN-CGLLPLAGPNIITKTKGLKWDVEDWPTSIESGNVSSSNRFVG 273
+L + G+ CGL+P+ P + T GLKW++ + + + VS+SN + G
Sbjct: 171 ---RLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGT-LVSTSNTYDG 223
>SPAC6F12.05c_2 [H] KOG3153 Thiamine pyrophosphokinase
Length = 233
Score = 79.0 bits (193), Expect = 8e-15
Identities = 71/257 (27%), Positives = 115/257 (44%), Gaps = 46/257 (17%)
Query: 38 LLILNQRIKLDHSAFEALWNSYTIKICADGAANRLFDYYRDSDGKRYHPDVIAGDMDSIR 97
+L+LNQ I + F LW +I++CADG AN+L +Y DS K PD + GD DS+
Sbjct: 19 VLLLNQPIDIPDDRFRTLWKRASIRVCADGGANQLRNY--DSSLK---PDYVVGDFDSLT 73
Query: 98 EDVLEYYEAIETTTVIKQNTQYSTDFTKSVNVATLLLLGIDLNAVEINEYDGIHKLYQQA 157
++ YY+ + ++ Q +TDF K + I E+ GI ++
Sbjct: 74 DETKAYYKEM-GVNIVFDPCQNTTDFMKCHKI--------------IKEH-GIDTIF--- 114
Query: 158 DKSQLKDIPLLILNGIDGRFDHTIHSMVQFY---ALRRQDPYFKMCFLTMSDMIILIPSS 214
+L G+ GR DH I ++ + ++ ++ F + L +S +L P
Sbjct: 115 -----------VLCGMGGRVDHAIGNLNHLFWAASISEKNEVFLLTELNVS--TLLQPGI 161
Query: 215 KKGYWLKLDKVKPLIGNCGLLPLAGPNIITKTKGLKWDVEDWPTSIESGNVSSSNRFVGE 274
+D + +CGLLP+ + KT GL+W++ED G VSS N
Sbjct: 162 N-----HVDCHDNIGLHCGLLPVGQSVYVKKTSGLEWNIEDRICQF-GGLVSSCNVVTKA 215
Query: 275 NCLIACEESIVMSVELK 291
I IV ++E +
Sbjct: 216 TVTIEVNNFIVWTMETR 232
>At1g02880 [H] KOG3153 Thiamine pyrophosphokinase
Length = 277
Score = 74.7 bits (182), Expect = 2e-13
Identities = 63/246 (25%), Positives = 108/246 (43%), Gaps = 43/246 (17%)
Query: 47 LDHSAFEALWNSYTIKICADGAANRLFDYYRDSDGKRYHPDVIAGDMDSIREDVLEYYEA 106
L S + LW T K D+ + + RY PDVI GDMDSIR DVL++Y
Sbjct: 63 LSSSLMKTLWPFETEK-----------DFMKKNLYFRYKPDVIKGDMDSIRRDVLDFYIN 111
Query: 107 IETTTVIKQNTQYSTDFTKSVNVATLLLLGIDLNAVEINEYDGIHKLYQQADKSQLKDIP 166
+ T + + + Q +TD K + L + LN + +
Sbjct: 112 LGTKVIDESHDQDTTDLDKCI----LYIRHSTLN-------------------QETSGLQ 148
Query: 167 LLILNGIDGRFDHTIHSMVQFYALRRQDPYFKMCFLTMSDMIILIPSSKKGYWLKLDKVK 226
+L + GRFDH ++ Y + P ++ L+ +I L+P + + ++
Sbjct: 149 ILATGALGGRFDHEAGNLNVLY----RYPDTRIVLLSDDCLIQLLPKTHRH---EIHIQS 201
Query: 227 PLIG-NCGLLPLAGPNIITKTKGLKWDVEDWPTSIESGNVSSSNRFVGENCLIACEESIV 285
L G +CGL+P+ P+ T T GL+WD+ + G +S+SN E + + ++
Sbjct: 202 SLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRF-GGLISTSNLVKEEKITVESDSDLL 260
Query: 286 MSVELK 291
++ +K
Sbjct: 261 WTISIK 266
>7299502 [H] KOG3153 Thiamine pyrophosphokinase
Length = 332
Score = 57.8 bits (138), Expect = 2e-08
Identities = 59/264 (22%), Positives = 113/264 (42%), Gaps = 45/264 (17%)
Query: 37 VLLILNQRIKLDHSAFEALWNSYTIKICADGAANRLFDYY------RDSDGKR----YHP 86
V ++LN++I++ + LW + ++ DG +N D+ + ++G P
Sbjct: 87 VCVVLNRQIQVPAHVVKLLWKNAAVRCAVDGGSNHWRDFVVAQAMSKKANGSAPTTPLEP 146
Query: 87 -DVIAGDMDSIREDVLEYYEAIETTTVIKQNTQYSTDFTKSVNVATLLLLGIDLNAVEIN 145
DVI GD DSI E+ ++++ +TT + Q +TDFTK++ V
Sbjct: 147 LDVITGDFDSITEETVDFF---KTTPKVHTPDQDATDFTKAMAVL--------------- 188
Query: 146 EYDGIHKLYQQADKSQLKDIPLLILNGIDGRFDHTIHSMVQFYALRRQDPYFKMCFLTMS 205
Q +Q K +++ + GR D + ++ Y + Q + L+
Sbjct: 189 ----------QPVMTQRKIQDVVVFHDTSGRLDQVMANLNTLY--KSQKDNCNVFLLSGD 236
Query: 206 DMIILIPSSKKGYWLKLDKVKPLIGNCGLLPLAGPNIITKTKGLKWDVEDWPTSIE-SGN 264
+ L+ K + +D V C L+P+ T GLKW++ + +E G
Sbjct: 237 SVTWLLRPGKHTIQVPVDLVTSQRW-CSLMPVGSSAHNVTTTGLKWNL--YHAQLEFGGM 293
Query: 265 VSSSNRFVGENCLIACEESIVMSV 288
VS+SN + E + + +++ S+
Sbjct: 294 VSTSNTYATEFVQVETDANLIWSM 317
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,675,836
Number of Sequences: 60738
Number of extensions: 788930
Number of successful extensions: 1826
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1810
Number of HSP's gapped (non-prelim): 7
length of query: 304
length of database: 30,389,216
effective HSP length: 106
effective length of query: 198
effective length of database: 23,950,988
effective search space: 4742295624
effective search space used: 4742295624
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)