ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV1098    good          S     KOG3455  Function unknown                                               Predicted membrane protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV1098 381004 380558 -149 
         (149 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YER044c [S] KOG3455 Predicted membrane protein                        214   4e-56
SPBC337.09 [S] KOG3455 Predicted membrane protein                     113   8e-26
Hs6005719 [S] KOG3455 Predicted membrane protein                       81   6e-16
At1g10030 [S] KOG3455 Predicted membrane protein                       48   4e-06

>YER044c [S] KOG3455 Predicted membrane protein
          Length = 148

 Score =  214 bits (544), Expect = 4e-56
 Identities = 97/146 (66%), Positives = 123/146 (83%), Gaps = 1/146 (0%)

Query: 1   MLSFTEVSECVQKSLASLAPGYLSKWLLFISVVSIFNSVQTYIN-IDLTRRVYENKPNET 59
           M S  +V    + +LA++  GYL KWLLFIS+VS+FNS+QTY++ ++LTR+VYE KP ET
Sbjct: 1   MFSLQDVITTTKTTLAAMPKGYLPKWLLFISIVSVFNSIQTYVSGLELTRKVYERKPTET 60

Query: 60  TPLSARTFGTWTLVSCIIRLYGALYLTENHVYQLTFISYVIALLHFGSELLIFRTCKLGT 119
           T LSARTFGTWT +SC+IR YGA+YL E H+++L F+SY++AL HFGSELLIFRTCKLG 
Sbjct: 61  THLSARTFGTWTFISCVIRFYGAMYLNEPHIFELVFMSYMVALFHFGSELLIFRTCKLGK 120

Query: 120 GFLGPLVVSTTSLVWMYCQKEFYTGI 145
           GF+GPLVVSTTSLVWMY Q+E+YTG+
Sbjct: 121 GFMGPLVVSTTSLVWMYKQREYYTGV 146


>SPBC337.09 [S] KOG3455 Predicted membrane protein
          Length = 136

 Score =  113 bits (283), Expect = 8e-26
 Identities = 60/131 (45%), Positives = 82/131 (61%), Gaps = 1/131 (0%)

Query: 15  LASLAPGYLSKWLLFISVVSIFNSVQTYINIDLTRRVYENKPNETTPLSARTFGTWTLVS 74
           LA L    ++KW + +SV ++FN+VQ+++   LT+RVY N  NE   L  RTFG WTL+S
Sbjct: 5   LAMLPDSLVAKWNVVVSVAALFNTVQSFLTPKLTKRVYSNT-NEVNGLQGRTFGIWTLLS 63

Query: 75  CIIRLYGALYLTENHVYQLTFISYVIALLHFGSELLIFRTCKLGTGFLGPLVVSTTSLVW 134
            I+R Y A ++T   VY L   +Y +A  HF SE L+FRT  LG G L P+VVST S+ +
Sbjct: 64  AIVRFYCAYHITNPDVYFLCQCTYYLACFHFLSEWLLFRTTNLGPGLLSPIVVSTVSIWF 123

Query: 135 MYCQKEFYTGI 145
           M  +K    GI
Sbjct: 124 MAKEKASILGI 134


>Hs6005719 [S] KOG3455 Predicted membrane protein
          Length = 140

 Score = 80.9 bits (198), Expect = 6e-16
 Identities = 41/114 (35%), Positives = 71/114 (61%), Gaps = 1/114 (0%)

Query: 23  LSKWLLFISVVSIFNSVQTYINID-LTRRVYENKPNETTPLSARTFGTWTLVSCIIRLYG 81
           L  WL+ +S++++ N++Q++ +   L  ++Y  KPN    L ARTFG WTL+S +IR   
Sbjct: 8   LRSWLVMVSIIAMGNTLQSFRDHTFLYEKLYTGKPNLVNGLQARTFGIWTLLSSVIRCLC 67

Query: 82  ALYLTENHVYQLTFISYVIALLHFGSELLIFRTCKLGTGFLGPLVVSTTSLVWM 135
           A+ +    +Y +T  ++++AL HF SEL ++ T     G L PL+V++ S++ M
Sbjct: 68  AIDIHNKTLYHITLWTFLLALGHFLSELFVYGTAAPTIGVLAPLMVASFSILGM 121


>At1g10030 [S] KOG3455 Predicted membrane protein
          Length = 110

 Score = 48.1 bits (113), Expect = 4e-06
 Identities = 27/92 (29%), Positives = 47/92 (50%), Gaps = 1/92 (1%)

Query: 23  LSKWLLFISVVSIFNSVQTYINIDLTRRVYENKPNETTPLSARTFGTWTLVSCIIRLYGA 82
           L  WL+ +  + + +    + NI   R    ++    + +  RTFG WTL++C +    A
Sbjct: 4   LGYWLMVVGSLRLASVWFGFFNIWALRLAVFSQTT-MSEVHGRTFGVWTLLTCTLCFLCA 62

Query: 83  LYLTENHVYQLTFISYVIALLHFGSELLIFRT 114
             L    +Y  TF+S++ AL HF +E L ++T
Sbjct: 63  FNLENKPLYLATFLSFIYALGHFLTEYLFYQT 94


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.327    0.140    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,768,249
Number of Sequences: 60738
Number of extensions: 324600
Number of successful extensions: 879
Number of sequences better than 1.0e-05: 4
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 874
Number of HSP's gapped (non-prelim): 4
length of query: 149
length of database: 30,389,216
effective HSP length: 96
effective length of query: 53
effective length of database: 24,558,368
effective search space: 1301593504
effective search space used: 1301593504
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)