ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1218 good L KOG3818 Replication, recombination and repair DNA polymerase epsilon, subunit B
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1218 424886 427015 710
(710 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR175w [L] KOG3818 DNA polymerase epsilon subunit B 715 0.0
SPBP8B7.14c [L] KOG3818 DNA polymerase epsilon subunit B 292 1e-78
Hs4505935 [L] KOG3818 DNA polymerase epsilon subunit B 164 3e-40
7295418 [L] KOG3818 DNA polymerase epsilon subunit B 139 1e-32
CE07046 [L] KOG3818 DNA polymerase epsilon subunit B 108 3e-23
>YPR175w [L] KOG3818 DNA polymerase epsilon subunit B
Length = 692
Score = 715 bits (1846), Expect = 0.0
Identities = 367/711 (51%), Positives = 490/711 (68%), Gaps = 31/711 (4%)
Query: 2 AGVVLPASIQPQLLRPLAYRILTKKFGLNIKSDGLVALAAYIGTVFGLYWRQNSETTKFL 61
+G VLP IQP LLRPLAYR+L++K+GL+IKSDGL ALA ++GT G WRQ T KFL
Sbjct: 7 SGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATIKFL 66
Query: 62 EQFAIIWREQGRGLFVDELNVSQVINEIKEREKIEQDQHVEKRNNLHKANNLDAFLKRPN 121
EQFA +W++Q RGLF+D+ V +VI E+KEREK+E H + + L R +
Sbjct: 67 EQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHE-------HPIQHEENILGRTD 119
Query: 122 SPTDTEITTLSQGSATSVVNPDSHSPMMLEEGSPINSDS--EPISEHERANGISNVDQQL 179
+ + + +S+ N SPM P D +P + L
Sbjct: 120 DDENNSDDEMPIAADSSLQNVSLSSPMR----QPTERDEYKQPFKPES--------SKAL 167
Query: 180 DWLDYFKVIDAFNQQTFTYDHTKRQYIYVPRPKEVEKNMLSIAKLKLPNVESKVSLFTTR 239
DW DYFKVI+A QQ F+Y+ K Q+I+VP K+ N L LP++E KV +F TR
Sbjct: 168 DWRDYFKVINASQQQRFSYNPHKMQFIFVPNKKQ---NGLGGIAGFLPDIEDKVQMFLTR 224
Query: 240 YHIIKDKVLRNEKFQNNDIFNPLSSIIEMGKHMNESDVSPLNSASYMKITQIKNLLGHDG 299
Y++ D+V+RNE FQN+D+FNPLSS++ + + S+ + +S M IT IKNLLG D
Sbjct: 225 YYLTNDRVMRNENFQNSDMFNPLSSMVSLQNEL--SNTNRQQQSSSMSITPIKNLLGRDA 282
Query: 300 KNFLLLGLLDQNSKGNWSLEDPSGSIELHLQQAIPTKGTYYVPGCIVLVEGIYYTASNTF 359
+NFLLLGLL++N KGNWSLEDPSGS+E+ + Q IPT+G YYVPGC+VLVEGIYY+ N F
Sbjct: 283 QNFLLLGLLNKNFKGNWSLEDPSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKF 342
Query: 360 VVSSITHPPGEKREDTIEAIGNLDLLGAYNPSNENYVARLDKDLKIRLHYLEQELSDNKF 419
V+S+T PPGE+RE T+E IGNLDLLG + SN N++ARLDKDLKIRLH LE+EL+D+KF
Sbjct: 343 HVTSMTLPPGERREITLETIGNLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKF 402
Query: 420 VILGGDIYLDEMTTMDGLKKTFDKLTTDPPVAIVFNGSFVSVPVHPSLNAKNVSATVSYK 479
VILG +++LD++ M L K KL DPP +++ GSF SVPV S++++N+S++ +K
Sbjct: 403 VILGANLFLDDLKIMTALSKILQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFK 462
Query: 480 NNFDALATLLSNYENLINDTHMIFIPGPNDPWTSMVGLGTTTMWPQKIIPSSFTQKMSRI 539
NNFDALATLLS ++NL +T MIFIPGPND W SMV LG + PQ IPS+FT+K++++
Sbjct: 463 NNFDALATLLSRFDNLTENTTMIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKV 522
Query: 540 CRKVHWGSNPLRIAYLSQEIVLTRDDLANRFKRYNIVFPTVE-EEKYLENAELQEQYSRN 598
C+ V W SNP RIAYLSQEIV+ RDDL+ RFKR+ + FP E E+ Y EN + S++
Sbjct: 523 CKNVVWSSNPTRIAYLSQEIVIFRDDLSGRFKRHRLEFPFNESEDVYTENDNMM---SKD 579
Query: 599 PD-VSVGQLIAFKNNLPVSVLESRKLVKTLLDQQHLSPFSSRIRPTVWDLDFTLQLSPLP 657
D V + +L+ + LP V E+RKLVKT+LDQ HLSPF +RP WDLD TL L P+P
Sbjct: 580 TDIVPIDELVKEPDQLPQKVQETRKLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIP 639
Query: 658 SSIMLCDTSAPAFDVTYNGCKTINPGRFIHKRAAKYIEFFPASKVLVEEEL 708
S+++LCDT++ FD+TYNGCK INPG FIH R A+Y+E+ P+SK ++EE+
Sbjct: 640 STMVLCDTTSAQFDLTYNGCKVINPGSFIHNRRARYMEYVPSSKKTIQEEI 690
>SPBP8B7.14c [L] KOG3818 DNA polymerase epsilon subunit B
Length = 594
Score = 292 bits (748), Expect = 1e-78
Identities = 202/698 (28%), Positives = 317/698 (44%), Gaps = 132/698 (18%)
Query: 10 IQPQLLRPLAYRILTKKFGLNIKSDGLVALAAYIGTVFGLYWRQNSETTKFLEQFAIIWR 69
+QP LRP+A+ + T+K+ LNI D L LA+++ G WR E L++ A W+
Sbjct: 20 VQPNALRPVAFHVFTRKYDLNINRDSLQELASFVSKKCGSQWRSQCEP--LLDEIAKTWK 77
Query: 70 EQGRGLFVDELNVSQVINEIKEREKIEQDQHVEKRNNLHKANNLDAFLKRPNSPTDTEIT 129
++ + Q + R L L N P + ++
Sbjct: 78 ------------------------RVHETQPIVTRPLLIPV------LANLNVPHEVRVS 107
Query: 130 TLSQGSATSVVNPDSHSPMMLEEGSPINSDSEPISEHERANGISNVDQQLDWLDYFKVID 189
+L++ GS +NS + I E + YF+V+D
Sbjct: 108 SLARVQTLETT------------GSFLNSSNSEIRETIKNE------------KYFRVLD 143
Query: 190 AFNQQTFTYDHTKRQYIYVPRPKEVEKNMLSIAKLKLPNVESKVSLFTTRYHIIKDKVLR 249
AF F YD +++ ++ + + + + + + R++++ ++LR
Sbjct: 144 AFKMPKFKYDSSRKVFVLSKQSPTL-----------MASASACTDMLNRRFNVVYSRILR 192
Query: 250 NEKFQNNDIFNPLSSIIEMGKHMNESDVSPLNSASYMKITQIKNLLGHDGKNFLLLGLLD 309
NE FQ S + +Y ++T I+NLLG G FLL GLL
Sbjct: 193 NESFQTPSFSGSFSQ-----------------TGTY-QLTPIRNLLGRAGNTFLLFGLLT 234
Query: 310 QNSKGNWSLEDPSGSIELHLQQAIPTKGTYYVPGCIVLVEGIYYTASNTFVVSSITHPPG 369
G LED ++L + QA G + PGC+VLV G + +S F+V + HPP
Sbjct: 235 IAPDGTLWLEDLDSQVQLDVSQAEQGFGLF-CPGCLVLVNG-QFLSSGLFLVFELGHPPI 292
Query: 370 EKREDTIEAIGNLDLLGAYNPSNENYVARLDKDLKIRLHYLEQELSDNKFVILGGDIYLD 429
E+R+ +++A+ NLD+LG +D L + EQ FV + +++LD
Sbjct: 293 ERRDASLKALNNLDILGL----------NMDAKQLALLRHAEQAYQSQAFVCIS-EVHLD 341
Query: 430 EMTTMDGLKKTFDKLTTDP--PVAIVFNGSFVSVPVHPSLNAKNVSATVSYKNNFDALAT 487
T L+K F K + P I+ GSF+S H N +++ YK F+ LA
Sbjct: 342 NHQTFYALEKIFQKYESSEAVPFCIILCGSFMSSAFH------NSGSSIQYKEGFNKLAA 395
Query: 488 LLSNYENLINDTHMIFIPGPNDPWTSMVGLGTTTMWPQKIIPSSFTQKMSRICRKVHWGS 547
L + + T IF+PGPNDPWT+ ++ P+ IP F ++ R+C+ + S
Sbjct: 396 SLEKFPKICEKTKFIFVPGPNDPWTT----NGISLMPKHSIPLHFVNRIQRVCKHTIFAS 451
Query: 548 NPLRIAYLSQEIVLTRDDLANRFKRYNIVF-PTVEEEKYLENAELQEQYSRNPDVSVGQL 606
NP RI + SQE+++ RDD++ RF+R ++ F T + + + L EQ
Sbjct: 452 NPCRIIFFSQEVLIYRDDISGRFQRNSLKFGKTPQGTSNINSIPLAEQ------------ 499
Query: 607 IAFKNNLPVSVLESRKLVKTLLDQQHLSPFSSRIRPTVWDLDFTLQLSPLPSSIMLCDTS 666
V + RKLVKT+LDQ HLSPF SR RP +WD D+ L + PLPS + L D+
Sbjct: 500 ---------QVHQQRKLVKTVLDQSHLSPFPSRTRPILWDFDYALSVFPLPSCMGLIDSE 550
Query: 667 APAFDVTYNGCKTINPGRFIHKRAAKYIEFFPASKVLV 704
+ AFDV Y GC T NPG + + E FP K ++
Sbjct: 551 SSAFDVHYGGCPTFNPGALLLGVHYNWQEVFPVKKEII 588
>Hs4505935 [L] KOG3818 DNA polymerase epsilon subunit B
Length = 526
Score = 164 bits (416), Expect = 3e-40
Identities = 140/537 (26%), Positives = 238/537 (44%), Gaps = 92/537 (17%)
Query: 174 NVDQQLDWLDYFKVIDAFNQQTFTYDHTKRQYIYVPRPKEVEKNMLSIAKLKLPNVESKV 233
+VD+ ++ + F +I AF+ F Y+ +++++ + N+ + K
Sbjct: 77 SVDETIEHV--FNIIGAFDIPRFVYNSERKKFLPLLMTNHPAPNLFGTPR-------DKA 127
Query: 234 SLFTTRYHIIKDKVLRNEKFQNNDIFNPLSSIIEMGKHMNESDVSPLNSASYMKITQIKN 293
+F RY I+ + R+E F I G H +ES S ++ I+
Sbjct: 128 EMFRERYTILHQRTHRHELFTPPVI----------GSHPDES-------GSKFQLKTIET 170
Query: 294 LLGHDGK--NFLLLGLLDQNSKGNWSLEDPSGSIELHLQQAIPTKGTYYVPGCIVLVEGI 351
LLG K + ++LG++ Q +G + LEDP+G+++L L +A G Y C VL EG
Sbjct: 171 LLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLY-TEACFVLAEGW 229
Query: 352 YYTASNTFVVSSITHPPGEKREDTIEAIGNLDLLGAYNPSNENYVARLDKDLKIRLHYLE 411
+ F V++ PP E T GN++ G PSN + +L LE
Sbjct: 230 F--EDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGG--PSNTSVKT------SAKLKQLE 279
Query: 412 QELSDNKFVILGGDIYLDEMTTMDGLKKTFDKLTTDPPVAIVFNGSFVSVPVHPSLNAKN 471
+E D FV L D++LD++ ++ L+ F + PP + G+F S P KN
Sbjct: 280 EENKDAMFVFLS-DVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPY-----GKN 333
Query: 472 VSATVSYKNNFDALATLLSNYENLINDTHMIFIPGPNDPWTSMVGLGTTTMWPQKIIPSS 531
+ K++ LA ++ Y + I+ + +F+PGP DP G ++ P+ + S
Sbjct: 334 --QVQALKDSLKTLADIICEYPD-IHQSRFVFVPGPEDP-------GFGSILPRPPLAES 383
Query: 532 FTQKMSRICRKVHWGSNPLRIAYLSQEIVLTRDDLANRFKRYNIVFPTVEEEKYLENAEL 591
T + + + +NP RI Y +QEI + R+DL N+ R + FP+
Sbjct: 384 ITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPS------------ 431
Query: 592 QEQYSRNPDVSVGQLIAFKNNLPVSVLESRKLVKTLLDQQHLSPFSSRIRPTVWDLDFTL 651
+NL + VKT+L Q HL+P + P W D+ L
Sbjct: 432 -------------------SNLAI----PNHFVKTILSQGHLTPLPLYVCPVYWAYDYAL 468
Query: 652 QLSPLPSSIMLCDTSAPAFDVTYNGCKTINPGRFIHKRAAKYIEFFPASKVLVEEEL 708
++ P+P +++ D P F T C INPG F + + F+P++K + + +L
Sbjct: 469 RVYPVPDLLVIADKYDP-FTTTNTECLCINPGSF-PRSGFSFKVFYPSNKTVEDSKL 523
>7295418 [L] KOG3818 DNA polymerase epsilon subunit B
Length = 525
Score = 139 bits (350), Expect = 1e-32
Identities = 135/527 (25%), Positives = 222/527 (41%), Gaps = 95/527 (18%)
Query: 185 FKVIDAFNQQTFTYDHTKRQYIYVPRPKEVEKNMLSIAKLKLPNVESKVSLFTTRYHIIK 244
F +IDAF F Y+ +++ +P+++ L P ++S RY ++
Sbjct: 88 FALIDAFTVPRFRYNQRIKKFELDTQPRQL---------LTAPRMKS--DYMQQRYAMLL 136
Query: 245 DKVLRNEKFQNNDIFNPLSSIIEMGKHMNESDVSPLNSASYMKITQIKNLLGHDG-KNFL 303
K LR+ D+F P ++I+ G V A K+ +NLL K +
Sbjct: 137 QKTLRH------DLFAP--AVIQDG-------VGAEAQAKKFKLQFAENLLATSAMKEAV 181
Query: 304 LLGLLDQNSKGNWSLEDPSGSIELHLQQAIPTKGTYYVPGCIVLVEGIYYTASNTFVVSS 363
+LGLL Q +G + +EDP+G ++L L A G ++ GC VL EG Y + V
Sbjct: 182 VLGLLTQLKEGKFYVEDPTGCVQLDLTGARFHAG-FFCEGCFVLAEGNYN--NGVLKVDG 238
Query: 364 ITHPPGEKREDTIEAIGNLDLLGAYNPSNENYVARLDKDLKIRLHYLEQELSDNKFVILG 423
+ PP E + G + G + Y A L + LE+ ++ V L
Sbjct: 239 LGFPPAEPANSSRAFFGTANTWGGESAKLLKYSAGLQE--------LERTNTETTIVFLS 290
Query: 424 GDIYLDEMTTMDGLKKTFDKLTTDPPVAIVFNGSFV-SVPVHPSLNAKNVSATVSYKNNF 482
D+ LD M+ L++ F + PP AIV G F S H L +++
Sbjct: 291 -DVRLDLPVVMEKLRQLFVGYDSCPPQAIVLMGPFTASTRNHHEL-----------RHHL 338
Query: 483 DALATLLSNYENLINDTHMIFIPGPNDPWTSMVGLGTTTMWPQKIIPSSFTQKMSRICRK 542
DAL L + E L T +I +P DP + P+ IP + + +
Sbjct: 339 DALGGLAAGCEQLKKQTDLILVPSSEDPTAPNI-------LPRAPIPECLAAGLLKAWPR 391
Query: 543 VHWGSNPLRIAYLSQEIVLTRDDLANRFKRYNIVFPTVEEEKYLENAELQEQYSRNPDVS 602
+NP R+ Y +Q+IV+ R DL +F R + FP + +++++ ++R
Sbjct: 392 TQLATNPCRLQYCTQQIVVCRLDLMAKFCRNTLHFPE-------DTSQIEQHFAR----- 439
Query: 603 VGQLIAFKNNLPVSVLESRKLVKTLLDQQHLSPFSSRIRPTVWDLDFTLQLSPLPSSIML 662
T++ Q HL P P WD D L L PLP I++
Sbjct: 440 -----------------------TIVCQGHLVPIHPIAMPVHWDYDPALWLYPLPDLIVM 476
Query: 663 CDTSAPAFDVTYNGCKTINPGRFIHKRAAKYIEFFPASKVLVEEELP 709
D S +F + +GC +N G F+ + A + + PA++ + + E+P
Sbjct: 477 GD-SCQSFSSSQHGCTVLNTGSFVKSKFA-FKVYIPATRTIEDSEIP 521
>CE07046 [L] KOG3818 DNA polymerase epsilon subunit B
Length = 526
Score = 108 bits (270), Expect = 3e-23
Identities = 113/441 (25%), Positives = 177/441 (39%), Gaps = 85/441 (19%)
Query: 286 MKITQIKN-------LLGHDGKNF---LLLGLLDQNSKGNWSLEDPSGSIELHLQQAIPT 335
M+ +KN +LG K+ ++LG+L Q + +ED SGS+E+ ++
Sbjct: 134 MRCASLKNFKFTTCEILGSSTKHLQSVVVLGMLTQQKADCYHIEDLSGSVEVEFKEDTKF 193
Query: 336 KGTYYVPGCIVLVEGIYYTASNTFVVSSITHPPGEKREDTIEAIGNLDLLGAYNPSNENY 395
+ I + EG + ++ V+ + P E E T + + SNEN+
Sbjct: 194 HHALFHEHSIAIFEGTF--ENSVLTVNEVAMVPVESAEVTRKELS----------SNENW 241
Query: 396 VARLDKDL-----KIRLHYLEQELSDNKFVILGGDIYLDEMTTMDGLKKTFDKLTTDPPV 450
DK ++R +QE D V L D++LD+ M + K PPV
Sbjct: 242 FGGDDKIAFRCSDRLRSALAKQE--DTSLVFLS-DVFLDDKKVMKAVFKLLQGYKDQPPV 298
Query: 451 AIVFNGSFVSVPVHPSLNAKNVSATVSYKNNFDALATLLSNYENLINDTHMIFIPGPNDP 510
AIVF G+F S P + F LA L+ T IF+PGP+DP
Sbjct: 299 AIVFCGNFCSRP-------RQTDTIDLLDRGFRWLANQLTPLRKDYEKTQFIFVPGPDDP 351
Query: 511 WTSMVGLGTTTMWPQKIIPSSFTQKMSRICRKVHWGSNPLRIAYLSQEIVLTRDDLANRF 570
+ T+ P+ +PS + +S I + SNP RI + SQEIV+ R DL +
Sbjct: 352 FVD-------TVLPRPHLPSLLFKHISPII-SCTFASNPCRIQFASQEIVVFRSDLIKKT 403
Query: 571 KRYNIVFPTVEEEKYLENAELQEQYSRNPDVSVGQLIAFKNNLPVSVLESRKLVKTLLDQ 630
R++I N++ V + SR +++L Q
Sbjct: 404 CRHSI-----------------------------------NSITVESIPSR-YARSVLSQ 427
Query: 631 QHLSPFSSRIRPTVWDLDFTLQLSPLPSSIMLCDTSAPAFDVTYNGCKTI--NPGRFIHK 688
HL P I P + D +L L PLP ++ D F G TI NPG F
Sbjct: 428 AHLCPLPQHITPVLPDFSHSLSLHPLPDLLITAD-RFETFTEKVVGADTIVSNPGSFSRS 486
Query: 689 RAAKYIEFFPASKVLVEEELP 709
++ ++P + ++P
Sbjct: 487 NYTFHV-YYPCQNRVEASQIP 506
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,554,473
Number of Sequences: 60738
Number of extensions: 2035908
Number of successful extensions: 5610
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 5578
Number of HSP's gapped (non-prelim): 8
length of query: 710
length of database: 30,389,216
effective HSP length: 114
effective length of query: 596
effective length of database: 23,465,084
effective search space: 13985190064
effective search space used: 13985190064
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)