ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1232 check: MH O KOG2014 Posttranslational modification, protein turnover, chaperones SMT3/SUMO-activating complex, AOS1/RAD31 component
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1232 432181 433137 319
(319 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR180w [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 comp... 350 1e-96
Hs4885585 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 co... 156 3e-38
SPAC4C5.04 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 c... 137 2e-32
7299631 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 comp... 134 1e-31
Hs20559018 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 c... 116 4e-26
At2g32410 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 comp... 91 2e-18
CE05449 [O] KOG2012 Ubiquitin activating enzyme UBA1 89 6e-18
At5g50680 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 co... 89 8e-18
At5g50580 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 co... 89 8e-18
At1g05180 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 comp... 88 2e-17
Hs4502169 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 comp... 87 4e-17
CE05246 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 comp... 83 5e-16
Hs4507763 [O] KOG2012 Ubiquitin activating enzyme UBA1 81 2e-15
YKL210w [O] KOG2012 Ubiquitin activating enzyme UBA1 80 3e-15
SPBC211.09 [O] KOG2012 Ubiquitin activating enzyme UBA1 79 1e-14
Hs21361718 [O] KOG2012 Ubiquitin activating enzyme UBA1 77 4e-14
7294767 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component 76 6e-14
7303864 [O] KOG2012 Ubiquitin activating enzyme UBA1 76 7e-14
Hs4507767 [O] KOG2012 Ubiquitin activating enzyme UBA1 74 2e-13
SPAC323.06c [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 co... 74 3e-13
At4g24940 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 co... 73 5e-13
ECU03g0930 [O] KOG2012 Ubiquitin activating enzyme UBA1 70 4e-12
At2g30110 [O] KOG2012 Ubiquitin activating enzyme UBA1 69 1e-11
7295159_1 [O] KOG2013 SMT3/SUMO-activating complex catalytic com... 65 2e-10
7303261 [O] KOG2015 NEDD8-activating complex catalytic component... 63 6e-10
At5g06460 [O] KOG2012 Ubiquitin activating enzyme UBA1 62 1e-09
SPBC16H5.03c [O] KOG2013 SMT3/SUMO-activating complex catalytic ... 60 4e-09
CE01166 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component 59 1e-08
Hs19923744 [O] KOG2015 NEDD8-activating complex catalytic compon... 58 2e-08
Hs4885649 [O] KOG2013 SMT3/SUMO-activating complex catalytic com... 58 2e-08
At5g19180 [O] KOG2015 NEDD8-activating complex catalytic compone... 57 3e-08
At2g21470 [O] KOG2013 SMT3/SUMO-activating complex catalytic com... 57 3e-08
YDR390c [O] KOG2013 SMT3/SUMO-activating complex catalytic compo... 57 5e-08
CE25131 [O] KOG2013 SMT3/SUMO-activating complex catalytic compo... 57 5e-08
SPAC24H6.12c [O] KOG2015 NEDD8-activating complex catalytic comp... 54 3e-07
CE25130 [O] KOG2013 SMT3/SUMO-activating complex catalytic compo... 54 4e-07
YPR066w [O] KOG2015 NEDD8-activating complex catalytic component... 53 7e-07
ECU02g1340 [O] KOG2013 SMT3/SUMO-activating complex catalytic co... 51 3e-06
CE28320 [H] KOG2017 Molybdopterin synthase sulfurylase 50 4e-06
SPAC2G11.10c [H] KOG2017 Molybdopterin synthase sulfurylase 49 7e-06
CE04374 [O] KOG2015 NEDD8-activating complex catalytic component... 49 1e-05
>YPR180w [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 347
Score = 350 bits (899), Expect = 1e-96
Identities = 180/322 (55%), Positives = 244/322 (74%), Gaps = 4/322 (1%)
Query: 1 MTAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDI 60
MTAQANMRSA VLLINLGAIG+EITK+IVLSGIG LTILD H VTEEDLG+QFFIG +D+
Sbjct: 25 MTAQANMRSAKVLLINLGAIGSEITKSIVLSGIGHLTILDGHMVTEEDLGSQFFIGSEDV 84
Query: 61 GTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
G +++A + I+D+NPR++L D DLQ K++EFF QF+L+V T++ + K+N +TR
Sbjct: 85 GQWKIDATKERIQDLNPRIELNFDKQDLQEKDEEFFQQFDLVVATEMQIDEAIKINTLTR 144
Query: 121 ELNVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKDEEN 180
+LN+P+YVAG NGL Y+F DL+EFIS DEK++S RP +G S N+ II ++ RKDEE+
Sbjct: 145 KLNIPLYVAGSNGLFAYVFIDLIEFISEDEKLQSVRPTTVGPISSNRSIIEVTTRKDEED 204
Query: 181 -NAVYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQN---I 236
Y+ I TK+ Y+PL E+L +ATL ++++RQLKR+T +PLTL+LLQY Q I
Sbjct: 205 EKKTYERIKTKNCYRPLNEVLSTATLKEKMTQRQLKRVTSILPLTLSLLQYGLNQKGKAI 264
Query: 237 NLDDFRKKYDLTRKQLGLLIGEISNDTIEEFVEQAAVEISPVAAIIGGAVAQDVINILGK 296
+ + ++ + + LG+ + +D I++F++Q +E +PVAAIIGGAVAQDVINILGK
Sbjct: 265 SFEQMKRDAAVWCENLGVPATVVKDDYIQQFIKQKGIEFAPVAAIIGGAVAQDVINILGK 324
Query: 297 RQSPLNNFVVFDGTSLDMWVFE 318
R SPLNNF+VFDG +LDM +FE
Sbjct: 325 RLSPLNNFIVFDGITLDMPLFE 346
>Hs4885585 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 346
Score = 156 bits (395), Expect = 3e-38
Identities = 98/320 (30%), Positives = 166/320 (51%), Gaps = 25/320 (7%)
Query: 1 MTAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDI 60
+ AQ +R++ VLL+ L +G EI KN++L+G+ LT+LD VT ED GAQF I +
Sbjct: 28 LEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSV 87
Query: 61 GTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
G R EA+ +++NP V + VD D++ K + FF+QF+ + +T I K++++
Sbjct: 88 GRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICH 147
Query: 121 ELNVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKDEEN 180
+ ++ + + G GY F +L E +EK K A+ Q + P+ + L +
Sbjct: 148 KNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKL-------D 200
Query: 181 NAVYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQNINLDD 240
++ ++ K + P+KE L+ ++ ++ LKR T L LL++ T D
Sbjct: 201 SSETTMVKKKVVFCPVKEALE-VDWSSEKAKAALKRTTSDYFLLQVLLKFRT------DK 253
Query: 241 FRKKYDLTRKQLGLLIGEISNDTI-----------EEFVEQAAVEISPVAAIIGGAVAQD 289
R T ++ L+ +I ND + E+FV E++PV A++GG +AQ+
Sbjct: 254 GRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQE 313
Query: 290 VINILGKRQSPLNNFVVFDG 309
++ L +R P NNF FDG
Sbjct: 314 IVKALSQRDPPHNNFFFFDG 333
>SPAC4C5.04 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 307
Score = 137 bits (345), Expect = 2e-32
Identities = 90/309 (29%), Positives = 153/309 (49%), Gaps = 34/309 (11%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
AQ ++ + VLLI + NEI KN+VLSGIG L +LDS V E+D+ QFFI DIG
Sbjct: 25 AQQALKQSRVLLITASPLANEIAKNLVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQ 84
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTREL 122
R ++ + ++NP V++ D S + ++ S+F++++ T L + ++NE+TR
Sbjct: 85 LRANVFKKKLHELNPLVEIDTDTSLISEIDEGKISKFSMVIATQLDYEEFCRINELTRIC 144
Query: 123 NVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKDEENNA 182
N Y GL G+ F DL+ ++V + K EE+
Sbjct: 145 NASFYATSCFGLYGFAFCDLINHNFAIDRV-------------------VDNTKVEEDMF 185
Query: 183 VYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQNINLDDFR 242
+ Q KP+KE +S L L R K++ P L+LL+ + ++
Sbjct: 186 IVQ--------KPMKEAFQS-ILGETLKPRLAKKIPTLYPAMLSLLKSKKSDPDSIRQVC 236
Query: 243 KKYDLTRKQLGLLIGEISNDTIEEFVEQAAVEISPVAAIIGGAVAQDVINILGKRQSPLN 302
+ L K + ++ + + +F + + +PV +++GG V+QD +N + K+Q P++
Sbjct: 237 IEQKLNEKTV------LNGEFLSKFSSNISFQWTPVMSVVGGVVSQDALNSISKKQFPID 290
Query: 303 NFVVFDGTS 311
NF +FD S
Sbjct: 291 NFWIFDAES 299
>7299631 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 337
Score = 134 bits (338), Expect = 1e-31
Identities = 90/313 (28%), Positives = 152/313 (47%), Gaps = 25/313 (7%)
Query: 1 MTAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDI 60
+ +Q +R+A +L+ L +G EITKNI+LSG+ S+ +LD DVTEED +QF + ++ +
Sbjct: 31 LESQKRLRTAKILIAGLCGLGAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESL 90
Query: 61 GTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
T R EA+ +NP V ++ D L+ K EFF QF+++V+ ++ +++ + R
Sbjct: 91 NTNRAEASLTRARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150
Query: 121 ELNVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKDEEN 180
+L V + G G+ F L + ++ +K +K + N ++K E
Sbjct: 151 DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIK------------HKVVANSEKKKKYE- 197
Query: 181 NAVYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQNINLDD 240
+ T + P + R+LKR P V L L ++ T +
Sbjct: 198 --TVSIPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHKRD--- 252
Query: 241 FRKKYDLTRKQLGLLIG---EISNDTI--EEFVEQAAVEISPVAAIIGGAVAQDVINILG 295
Y L LL G E+ ++I +E + +ISP A++GG VAQ+VI ++
Sbjct: 253 --PSYKTREADLELLRGIRDELLPNSILGDEALGLIFAQISPAVAVVGGVVAQEVIKVVT 310
Query: 296 KRQSPLNNFVVFD 308
K ++P N VFD
Sbjct: 311 KLEAPHRNLFVFD 323
>Hs20559018 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 377
Score = 116 bits (291), Expect = 4e-26
Identities = 79/301 (26%), Positives = 147/301 (48%), Gaps = 35/301 (11%)
Query: 12 VLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAARRH 71
VLL + +G EI KN++L+G+ LT+LD ++ E+ GAQF I +G R EA+
Sbjct: 88 VLLAGMKGLGAEIAKNLILAGVKGLTMLDHKQISPEEPGAQFLIRIGSVGRNRAEASLER 147
Query: 72 IEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTRELNVPIYVAGI 131
+++NP V + +D D++ K + FF+QF+ + +++ + ++ + +
Sbjct: 148 AQNLNPMVDVKLDTEDIEKKPESFFTQFDAV-------------DQICHKNSIKFFAGDV 194
Query: 132 NGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKDEENNAVYQVITTKH 191
GY F +L E +EK K A+ Q + P+ + + L +++ ++ K
Sbjct: 195 FSYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRVKL-------DSSETTMVKKKV 247
Query: 192 TYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQNINLDDFRKKYDLTRKQ 251
+ P+KE L+ ++ ++ LKR T L LL++ T D R T +
Sbjct: 248 VFCPVKEALE-VDWSSKKAKAALKRTTSDHFLLQVLLKFRT------DKGRDPSSDTHGE 300
Query: 252 LGLLIGEISNDTIE--------EFVEQAAVEISPVAAIIGGAVAQDVINILGKRQSPLNN 303
L+ +I ND ++ F+ E++PV A++GG +AQ+++ L ++ P N
Sbjct: 301 DSELLLQIRNDVLDSLGIIPDPRFITYFFSEMAPVCAVVGGILAQEIVKALSQQDPPHNF 360
Query: 304 F 304
F
Sbjct: 361 F 361
>At2g32410 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 523
Score = 90.9 bits (224), Expect = 2e-18
Identities = 50/151 (33%), Positives = 83/151 (54%), Gaps = 3/151 (1%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
Q+ + +AS+ L+N G G+E KN+V+ GIGS+TI+D V DLG F + +G
Sbjct: 20 QSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQS 79
Query: 64 RLEAARRHIEDMNPRVK---LTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
R + ++++N VK + + L S + FFSQF L++ T L + KL+ + R
Sbjct: 80 RAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQLVEDSMVKLDRICR 139
Query: 121 ELNVPIYVAGINGLSGYIFTDLVEFISTDEK 151
E NV + +A GL+G++ + E + + K
Sbjct: 140 EANVMLVLARSYGLTGFVRISVKEHTAIETK 170
>CE05449 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1113
Score = 89.4 bits (220), Expect = 6e-18
Identities = 89/357 (24%), Positives = 159/357 (43%), Gaps = 53/357 (14%)
Query: 2 TAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIG 61
+A N+R+ASVL+ LG++G EI KN++L G+ +TI D+ DL AQ+++ D+G
Sbjct: 118 SAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVG 177
Query: 62 TKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTRE 121
R + + ++N V + V +L +EF F+L+V+TD ++ TR
Sbjct: 178 HNRATSCYERLAELNDSVNVQVSTDEL---TEEFVKTFDLVVLTDAARTAQRQIAAWTRA 234
Query: 122 LNVPIYVAGINGLSGYIFTDLVEFI----STDEKVKSARPEQLGKQSPNKEII-NLSERK 176
N I + G+ YIF D + +T E+V+ E + K + + NL
Sbjct: 235 HNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGL 294
Query: 177 DEENNAVYQVI---TTKHTYKPLKELLKSATLIN------------QLSR-RQLKRMT-- 218
++ ++ + + T + +PLK +K+A+ N + R RQ+K T
Sbjct: 295 EDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSV 354
Query: 219 PKVPLTLTLLQ-------YETFQNIN--------LDDFRKKYD------------LTRKQ 251
VP +L++ Y F+ + L F +KY L ++
Sbjct: 355 SHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKEL 414
Query: 252 LGLLIGEISNDTIEEFVEQAAVEISPVAAIIGGAVAQDVINILGKRQSPLNNFVVFD 308
+ EI IE F A+ + V++++GG AQ+ + + +PL ++ D
Sbjct: 415 IPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLD 471
>At5g50680 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 320
Score = 89.0 bits (219), Expect = 8e-18
Identities = 80/311 (25%), Positives = 131/311 (41%), Gaps = 35/311 (11%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDD--- 59
AQ + + VL+ + E KNIVL+G+GS+T+LD VT E A F I D+
Sbjct: 25 AQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAY 84
Query: 60 IGTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVT 119
+G E ++D NP V ++++ DL + +FF +F+++VI A + +NE
Sbjct: 85 VGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144
Query: 120 REL--NVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKD 177
R L +V Y G G IF DL + T +K+ +L S + + +
Sbjct: 145 RNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTKKKLDETVECELTFPSFEEAVSVPWKPMP 204
Query: 178 EENNAVYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQNIN 237
+Y + ++ + ++ L R + L L + + +
Sbjct: 205 RRTAKLYFAMRVIELFEETEGRKPGECSLSDLPR--------VLKLKKELCEGNSVSENH 256
Query: 238 LDDFRKKYDLTRKQLGLLIGEISNDTIEEFVEQAAVEISPVAAIIGGAVAQDVINILGKR 297
+ D LL +SN+T E P AIIGG + Q+VI ++ +
Sbjct: 257 IPDI------------LLERLVSNNT----------EFPPACAIIGGILGQEVIKVISGK 294
Query: 298 QSPLNNFVVFD 308
PL NF FD
Sbjct: 295 GEPLKNFFYFD 305
>At5g50580 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 320
Score = 89.0 bits (219), Expect = 8e-18
Identities = 80/311 (25%), Positives = 131/311 (41%), Gaps = 35/311 (11%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDD--- 59
AQ + + VL+ + E KNIVL+G+GS+T+LD VT E A F I D+
Sbjct: 25 AQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAY 84
Query: 60 IGTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVT 119
+G E ++D NP V ++++ DL + +FF +F+++VI A + +NE
Sbjct: 85 VGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144
Query: 120 REL--NVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKD 177
R L +V Y G G IF DL + T +K+ +L S + + +
Sbjct: 145 RNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTKKKLDETVECELTFPSFEEAVSVPWKPMP 204
Query: 178 EENNAVYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQNIN 237
+Y + ++ + ++ L R + L L + + +
Sbjct: 205 RRTAKLYFAMRVIELFEETEGRKPGECSLSDLPR--------VLKLKKELCEGNSVSENH 256
Query: 238 LDDFRKKYDLTRKQLGLLIGEISNDTIEEFVEQAAVEISPVAAIIGGAVAQDVINILGKR 297
+ D LL +SN+T E P AIIGG + Q+VI ++ +
Sbjct: 257 IPDI------------LLERLVSNNT----------EFPPACAIIGGILGQEVIKVISGK 294
Query: 298 QSPLNNFVVFD 308
PL NF FD
Sbjct: 295 GEPLKNFFYFD 305
>At1g05180 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 540
Score = 87.8 bits (216), Expect = 2e-17
Identities = 47/151 (31%), Positives = 80/151 (52%), Gaps = 3/151 (1%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
QA + AS+ L+N G G+E KN+VL G+GS+T++D V DLG F + +G
Sbjct: 36 QAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKVQFGDLGNNFMVDAKSVGQS 95
Query: 64 RLEAARRHIEDMNPRVK---LTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
+ ++ ++++N V + + L + N FFSQF L++ T L + KL+ + R
Sbjct: 96 KAKSVCAFLQELNDSVNAKFIEENPDTLITTNPSFFSQFTLVIATQLVEDSMLKLDRICR 155
Query: 121 ELNVPIYVAGINGLSGYIFTDLVEFISTDEK 151
+ NV + + GL+G++ + E D K
Sbjct: 156 DANVKLVLVRSYGLAGFVRISVKEHPIIDSK 186
>Hs4502169 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 534
Score = 86.7 bits (213), Expect = 4e-17
Identities = 50/138 (36%), Positives = 74/138 (53%), Gaps = 3/138 (2%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
Q + SA V LIN A G EI KN+VL GIGS TI+D + V+ ED G FF+ + IG
Sbjct: 25 QEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKN 84
Query: 64 RLEAARRHIEDMNPRVK---LTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
R EAA ++++N V + +L + FF +F ++V T L + +L +V
Sbjct: 85 RAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLW 144
Query: 121 ELNVPIYVAGINGLSGYI 138
+P+ + GL GY+
Sbjct: 145 NSQIPLLICRTYGLVGYM 162
>CE05246 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 343
Score = 83.2 bits (204), Expect = 5e-16
Identities = 73/321 (22%), Positives = 138/321 (42%), Gaps = 22/321 (6%)
Query: 1 MTAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGK--D 58
M AQ +R++ VL+I +G E+ K + L+G+ + ++D V E++G F D
Sbjct: 20 MEAQNKIRNSKVLIIGGKQLGAEVAKTLSLAGVDEMHLVDHRLVDTEEIGMNFLYDASVD 79
Query: 59 DIGTKRLEAARRHIEDMNPRVKLTVDISDLQSKN----KEFFSQFNLIVITDLFPADIEK 114
+ + A+ + ++N VKL + D+ SKN +E+ ++F L+V+ D K
Sbjct: 80 NSKMTKWAASYNFLYNLNRNVKLFIVEEDVLSKNDSEIEEYLTKFTLVVVLDESYERTAK 139
Query: 115 LNEVTRELNVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSE 174
+N + R+ ++ I G GY F D F VK+ P+ L ++ +
Sbjct: 140 VNNICRKHHIRFISGAIYGWIGYAFFD---FDGHAYLVKAKSPDCLNEEESETGKTSTVV 196
Query: 175 RKDEENNAVYQVITTKHTYKPLKELLKSATLINQLSRRQLKRMTPKVPLTLTLLQYETFQ 234
DEE + +Y E L S ++ R+ KR+ P + + + +
Sbjct: 197 TVDEE------FVLETFSYPSFVETLNSDFTAKKIVRK-CKRIVPTSYFLVKSMLRASSE 249
Query: 235 N----INLDDFRKKYDLTRKQLGLLIGEISNDTIE--EFVEQAAVEISPVAAIIGGAVAQ 288
N + +D K + +++ I ++ F P AA +GG + Q
Sbjct: 250 NKLTGVTENDIEKLIPIWNEEVAAGNHTIDMQPVQPDRFDHLFGPNFGPTAACVGGVIGQ 309
Query: 289 DVINILGKRQSPLNNFVVFDG 309
+ I + + ++PL N ++ G
Sbjct: 310 EAIKSISEGKNPLRNLFIYTG 330
>Hs4507763 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1058
Score = 81.3 bits (199), Expect = 2e-15
Identities = 52/167 (31%), Positives = 88/167 (52%), Gaps = 7/167 (4%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A ++++SVL+ L +G EI KNI+L G+ ++T+ D DL +QF++ ++DIG
Sbjct: 66 AMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGK 125
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTREL 122
R E ++ + ++N V +T L ++F S F ++V+T+ D ++ E
Sbjct: 126 NRAEVSQPRLAELNSYVPVTAYTGPLV---EDFLSGFQVVVLTNTPLEDQLRVGEFCHNR 182
Query: 123 NVPIYVAGINGLSGYIFTDL-VEFISTD---EKVKSARPEQLGKQSP 165
+ + VAG GL G +F D E I TD E+ SA + K +P
Sbjct: 183 GIKLVVAGTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNP 229
>YKL210w [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1024
Score = 80.5 bits (197), Expect = 3e-15
Identities = 57/185 (30%), Positives = 90/185 (47%), Gaps = 7/185 (3%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A M++++VL++ L +G EI KN+VL+G+ S+T+ D V DL QFF+ + DIG
Sbjct: 30 AMLKMQTSNVLILGLKGLGVEIAKNVVLAGVKSMTVFDPEPVQLADLSTQFFLTEKDIGQ 89
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFP-ADIEKLNEVTRE 121
KR + R + ++N V + V + L + SQF ++V TD D K+NE
Sbjct: 90 KRGDVTRAKLAELNAYVPVNV-LDSLDDVTQ--LSQFQVVVATDTVSLEDKVKINEFCHS 146
Query: 122 LNVPIYVAGINGLSGYIFTDL-VEFISTDEKVKSARPEQLGKQSPNKEIINLSERKD--E 178
+ + GL G F DL EF D + R + P+ + L + + E
Sbjct: 147 SGIRFISSETRGLFGNTFVDLGDEFTVLDPTGEEPRTGMVSDIEPDGTVTMLDDNRHGLE 206
Query: 179 ENNAV 183
+ N V
Sbjct: 207 DGNFV 211
Score = 48.9 bits (115), Expect = 1e-05
Identities = 29/101 (28%), Positives = 50/101 (48%), Gaps = 7/101 (6%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGS-----LTILDSHDVTEEDLGAQFFIGKD 58
Q + ++ V L+ GAIG E+ KN L G+GS + + D+ + + +L QF
Sbjct: 429 QKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIEKSNLNRQFLFRPK 488
Query: 59 DIGTKRLEAARRHIEDMNP--RVKLTVDISDLQSKNKEFFS 97
D+G + E A + MNP + K+ I + + +E F+
Sbjct: 489 DVGKNKSEVAAEAVCAMNPDLKGKINAKIDKVGPETEEIFN 529
>SPBC211.09 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 644
Score = 78.6 bits (192), Expect = 1e-14
Identities = 46/148 (31%), Positives = 77/148 (51%), Gaps = 5/148 (3%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A M ++VL+I +G EI KN+ L+G+ S+T+ D EDL +Q+F+ +DDIG
Sbjct: 31 AMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGV 90
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTREL 122
R + + ++N V ++V + + E+ F +V+T+ ++N+ T +
Sbjct: 91 PRAKVTVSKLAELNQYVPVSV----VDELSTEYLKNFKCVVVTETSLTKQLEINDFTHKN 146
Query: 123 NVPIYVAGINGLSGYIFTDLVE-FISTD 149
++ A GL G IF D E FI TD
Sbjct: 147 HIAYIAADSRGLFGSIFCDFGENFICTD 174
>Hs21361718 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1052
Score = 76.6 bits (187), Expect = 4e-14
Identities = 47/145 (32%), Positives = 79/145 (54%), Gaps = 5/145 (3%)
Query: 2 TAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIG 61
TA M + V L +G +G EI KN+VL+GI ++TI D+ DLG FF+ +DD+
Sbjct: 54 TAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVV 113
Query: 62 TK--RLEAARRHIEDMNPRVKLT-VDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEV 118
K R EA +HI ++NP V +T + ++ + F ++ +V+T++ +K+N+
Sbjct: 114 NKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDF 173
Query: 119 TRELNVPI--YVAGINGLSGYIFTD 141
R PI A ++G+ +F D
Sbjct: 174 CRSQCPPIKFISADVHGIWSRLFCD 198
>7294767 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 524
Score = 76.3 bits (186), Expect = 6e-14
Identities = 45/138 (32%), Positives = 71/138 (50%), Gaps = 3/138 (2%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
Q + +A+V L+N+ A+G E K +VL GIG T+ D V EEDLG FF+ +G
Sbjct: 30 QTLLEAATVCLVNVTAVGCETAKGLVLPGIGGFTVADGSTVKEEDLGNNFFLDSSYLGKS 89
Query: 64 RLEAARRHIEDMNPRVK---LTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
+ A + ++++NP V + L + FF F+L++ ++L + L E
Sbjct: 90 KALACMQLLQELNPDVNGDYVDESADFLLANRPNFFDSFDLVIASNLNEQTLLLLAERLW 149
Query: 121 ELNVPIYVAGINGLSGYI 138
ELNVP+ G+ G I
Sbjct: 150 ELNVPLIYCRSLGMLGTI 167
>7303864 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1008
Score = 75.9 bits (185), Expect = 7e-14
Identities = 41/142 (28%), Positives = 76/142 (52%), Gaps = 3/142 (2%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A M ++ +LL LG +G EI KN++L G+ S+T+ D+ DL +QF++ + DIG
Sbjct: 28 AMRRMANSDILLSGLGGLGLEIAKNVILGGVKSITLHDTATCGLHDLSSQFYLTEADIGK 87
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTREL 122
R EA+ + ++N V+ +S +EF +F ++V+T+ + +++ + E
Sbjct: 88 NRAEASCAQLAELNNYVR---TVSHTGPLTEEFLRKFRVVVLTNSDGEEQQRIAKFAHEN 144
Query: 123 NVPIYVAGINGLSGYIFTDLVE 144
+ + +A GL +F D E
Sbjct: 145 GIALIIAETRGLFAKVFCDFGE 166
>Hs4507767 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1011
Score = 74.3 bits (181), Expect = 2e-13
Identities = 43/152 (28%), Positives = 75/152 (49%), Gaps = 3/152 (1%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A ++ A VL+ L +G E+ KN+VL G+GSLT+ D H DL AQF + + D+
Sbjct: 26 AMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLER 85
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTREL 122
R EA++ + +N V++ V D+ ++ F ++V+T + K+ + +
Sbjct: 86 SRAEASQELLAQLNRAVQVVVHTGDI---TEDLLLDFQVVVLTAAKLEEQLKVGTLCHKH 142
Query: 123 NVPIYVAGINGLSGYIFTDLVEFISTDEKVKS 154
V A GL G +F D E + + ++
Sbjct: 143 GVCFLAADTRGLVGQLFCDFGEDFTVQDPTEA 174
>SPAC323.06c [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 500
Score = 73.9 bits (180), Expect = 3e-13
Identities = 49/180 (27%), Positives = 86/180 (47%), Gaps = 5/180 (2%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
Q + + V L+ +G E KN++L GIGS ++D V G FFI D G
Sbjct: 22 QNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDFSMDGMNFFIQYDQEGKS 81
Query: 64 RLEAARRHIEDMNPRVK---LTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTR 120
R ++ +NP V+ L + L KN E+FS+F++++ ++L + +L E R
Sbjct: 82 RARCTASLLQQLNPNVEMEYLEMSPEALIDKNIEYFSKFSVVLSSNLKEKPLFRLEEYLR 141
Query: 121 ELNVPIYVAGINGLSGYIFTDLVEFISTDEKVKSARPEQLGKQSPNKEIINLSERKDEEN 180
+P+ G +G + E+ +T + + P+ L ++P E+IN + D +N
Sbjct: 142 SHKIPLLHFNSVGFAGILRISTHEYTTTQSQPE--LPQDLRLKNPWPELINYVKSMDLDN 199
>At4g24940 [O] KOG2014 SMT3/SUMO-activating complex AOS1/RAD31 component
Length = 319
Score = 73.2 bits (178), Expect = 5e-13
Identities = 48/148 (32%), Positives = 73/148 (48%), Gaps = 5/148 (3%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDD--- 59
AQ + A +L+ + E KNIVL+G+GS+T++D E L A F I D+
Sbjct: 25 AQRRLTKAHILVSGIKGTVAEFCKNIVLAGVGSVTLMDDRLANMEALNANFLIPPDENVY 84
Query: 60 IGTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVT 119
G E ++D NP V+++V+ DL +FF QF+++VI A + +NE
Sbjct: 85 SGKTVAEICSDSLKDFNPMVRVSVEKGDLSMLGTDFFEQFDVVVIGYGSRATKKYVNEKC 144
Query: 120 REL--NVPIYVAGINGLSGYIFTDLVEF 145
R+L V Y G IF DL ++
Sbjct: 145 RKLKKRVAFYTVDCRDSCGEIFVDLQDY 172
>ECU03g0930 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 991
Score = 70.1 bits (170), Expect = 4e-12
Identities = 44/148 (29%), Positives = 74/148 (49%), Gaps = 7/148 (4%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A M + VL++ L +G E+ KN+ L+GI + + D V+EEDL + F++ K+DIG
Sbjct: 25 AMKKMMGSKVLVMGLDGLGQEVVKNVCLAGISKVALFDDRAVSEEDLCSGFYLRKEDIGK 84
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTREL 122
R + MN V + V +SD+ S F ++++V + + +LNE+ R+
Sbjct: 85 PRDASVVGRFRSMNEYVDVDV-VSDVNS-----FEGYDIVVACNESYGEQIRLNEMARKD 138
Query: 123 NVPIYVAGINGLSGYIFTDL-VEFISTD 149
+ GL +F D EF+ D
Sbjct: 139 GCMFIGCQVRGLFSQVFCDFGAEFVCVD 166
>At2g30110 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1080
Score = 68.6 bits (166), Expect = 1e-11
Identities = 41/135 (30%), Positives = 74/135 (54%), Gaps = 5/135 (3%)
Query: 9 SASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAA 68
+++VL+ + +G EI KN++L+G+ S+T+ D V DL + F +DD+G R +A+
Sbjct: 95 ASNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADAS 154
Query: 69 RRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTRELNVPI-- 126
+ ++D+N V ++ S +S NKE S F ++V +D+ + ++ PI
Sbjct: 155 VQKLQDLNNAVVVS---SLTKSLNKEDLSGFQVVVFSDISMERAIEFDDYCHSHQPPIAF 211
Query: 127 YVAGINGLSGYIFTD 141
A + GL G +F D
Sbjct: 212 VKADVRGLFGSVFCD 226
>7295159_1 [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 731
Score = 64.7 bits (156), Expect = 2e-10
Identities = 38/139 (27%), Positives = 67/139 (47%), Gaps = 2/139 (1%)
Query: 2 TAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIG 61
T Q ++ + VL++ G IG E+ KN+VLSG + I+D + +L QF ++ +G
Sbjct: 12 TLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHVG 71
Query: 62 TKRLEAARRHIEDMNPRVKLTVDISDLQSKN--KEFFSQFNLIVITDLFPADIEKLNEVT 119
+ AR NP K+T + S + FF +F+L++ A +N +
Sbjct: 72 KSKARVARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLSALDNRAARNHVNRMC 131
Query: 120 RELNVPIYVAGINGLSGYI 138
+VP+ +G G +G +
Sbjct: 132 LNADVPLIESGTAGYNGQV 150
>7303261 [O] KOG2015 NEDD8-activating complex catalytic component UBA3
Length = 450
Score = 62.8 bits (151), Expect = 6e-10
Identities = 30/92 (32%), Positives = 50/92 (53%)
Query: 12 VLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAARRH 71
VL+I G +G E+ K++ L G G+L ++D + +L QF + DIG + E A R
Sbjct: 51 VLIIGAGGLGCELLKDLALMGFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARF 110
Query: 72 IEDMNPRVKLTVDISDLQSKNKEFFSQFNLIV 103
I P ++T +Q ++ F+ QF+L+V
Sbjct: 111 INARVPTCRVTPHFKKIQDFDESFYQQFHLVV 142
>At5g06460 [O] KOG2012 Ubiquitin activating enzyme UBA1
Length = 1077
Score = 61.6 bits (148), Expect = 1e-09
Identities = 42/139 (30%), Positives = 72/139 (51%), Gaps = 13/139 (9%)
Query: 9 SASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAA 68
+++VL+ + +G EI KNI+L+G+ S+T+ D + V DL + F ++DIG R A+
Sbjct: 92 ASNVLISGMQGLGVEIAKNIILAGVKSVTLHDENVVELWDLSSNFVFTEEDIGKNRALAS 151
Query: 69 RRHIEDMNPRVKLTVDISDLQSK-NKEFFSQFNLIVITDLFPADIEKLNEV-----TREL 122
++++N V +S L K KE S F ++V D+ EK E+ + +
Sbjct: 152 VHKLQELNN----AVAVSTLTGKLTKEQLSDFQVVVFVDI---SFEKATEIDDYCHSHQP 204
Query: 123 NVPIYVAGINGLSGYIFTD 141
+ A + GL G +F D
Sbjct: 205 PIAFIKADVRGLFGSLFCD 223
>SPBC16H5.03c [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 628
Score = 60.1 bits (144), Expect = 4e-09
Identities = 41/139 (29%), Positives = 68/139 (48%), Gaps = 4/139 (2%)
Query: 3 AQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGT 62
A N +SA VLL+ G IG E+ KN+++SG+ + I+D + +L QF K +
Sbjct: 19 ALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHVKQ 78
Query: 63 KRLEAARRHIEDMNPRVKLTVDISDLQSK--NKEFFSQFNLIVITDLFPADIEK-LNEVT 119
+ A + NP VKL ++++ N +F QF+L V L D + +N+
Sbjct: 79 PKAIVAAKTASSFNPNVKLEAYHANIKEDRFNVAWFRQFDL-VFNALDNLDARRHVNKQC 137
Query: 120 RELNVPIYVAGINGLSGYI 138
+VP+ +G G G +
Sbjct: 138 LLASVPLIESGTTGFLGQV 156
>CE01166 [O] KOG2016 NEDD8-activating complex APP-BP1/UBA5 component
Length = 525
Score = 58.5 bits (140), Expect = 1e-08
Identities = 27/76 (35%), Positives = 45/76 (58%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
QA++ S S ++ ++ EI K++VL+G+ S ++D V + D+G FF+ DDIG
Sbjct: 22 QASIGSTSACVLGSDSLATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLHADDIGRS 81
Query: 64 RLEAARRHIEDMNPRV 79
R EA + ++NP V
Sbjct: 82 RAEATLEKLTELNPSV 97
>Hs19923744 [O] KOG2015 NEDD8-activating complex catalytic component UBA3
Length = 463
Score = 58.2 bits (139), Expect = 2e-08
Identities = 29/95 (30%), Positives = 46/95 (47%)
Query: 9 SASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAA 68
+ VL+I G +G E+ KN+ LSG + ++D + +L QF DIG + E A
Sbjct: 69 TCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVA 128
Query: 69 RRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIV 103
+ D P + +Q N F+ QF++IV
Sbjct: 129 AEFLNDRVPNCNVVPHFYKIQDFNDTFYRQFHIIV 163
>Hs4885649 [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 640
Score = 57.8 bits (138), Expect = 2e-08
Identities = 34/131 (25%), Positives = 61/131 (45%), Gaps = 2/131 (1%)
Query: 12 VLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAARRH 71
VL++ G IG E+ KN+VL+G + ++D + +L QF K +G + + A+
Sbjct: 20 VLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKES 79
Query: 72 IEDMNPRVKLTVDISDLQSK--NKEFFSQFNLIVITDLFPADIEKLNEVTRELNVPIYVA 129
+ P+ + + + N EFF QF L++ A +N + +VP+ +
Sbjct: 80 VLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIES 139
Query: 130 GINGLSGYIFT 140
G G G + T
Sbjct: 140 GTAGYLGQVTT 150
>At5g19180 [O] KOG2015 NEDD8-activating complex catalytic component UBA3
Length = 477
Score = 57.4 bits (137), Expect = 3e-08
Identities = 26/93 (27%), Positives = 51/93 (53%)
Query: 12 VLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAARRH 71
+L+I G +G E+ K++ LSG +L ++D + +L QF +D+G + E A +
Sbjct: 49 ILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKR 108
Query: 72 IEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVI 104
+ + V++ S ++ K EF++ FN+I +
Sbjct: 109 VMERVSGVEIVPHFSRIEDKEIEFYNDFNIIAL 141
>At2g21470 [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 700
Score = 57.4 bits (137), Expect = 3e-08
Identities = 34/138 (24%), Positives = 69/138 (49%), Gaps = 4/138 (2%)
Query: 4 QANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTK 63
Q+ ++ A VL++ G IG E+ K + LSG + I+D + +L QF + +G
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQS 66
Query: 64 RLEAARRHIEDMNPRVKLTVDISDLQSK--NKEFFSQFNLIVITDLFPADIEK-LNEVTR 120
+ + AR + P + + +++++ + +FF QF+ +V+ L D + +N +
Sbjct: 67 KAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQFD-VVLNGLDNLDARRHVNRLCL 125
Query: 121 ELNVPIYVAGINGLSGYI 138
+VP+ +G G G +
Sbjct: 126 AADVPLVESGTTGFLGQV 143
>YDR390c [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 636
Score = 56.6 bits (135), Expect = 5e-08
Identities = 35/134 (26%), Positives = 68/134 (50%), Gaps = 2/134 (1%)
Query: 7 MRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLE 66
+RS+ LL+ G IG+E+ K+I+L G + I+D + +L QF + DI +
Sbjct: 19 LRSSRCLLVGAGGIGSELLKDIILMEFGEIHIVDLDTIDLSNLNRQFLFRQKDIKQPKST 78
Query: 67 AARRHIEDMN--PRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKLNEVTRELNV 124
A + ++ N V ++ D+ + +F QF++I A +N++++ L++
Sbjct: 79 TAVKAVQHFNNSKLVPYQGNVMDISTFPLHWFEQFDIIFNALDNLAARRYVNKISQFLSL 138
Query: 125 PIYVAGINGLSGYI 138
P+ +G G GY+
Sbjct: 139 PLIESGTAGFDGYM 152
>CE25131 [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 438
Score = 56.6 bits (135), Expect = 5e-08
Identities = 31/131 (23%), Positives = 64/131 (48%), Gaps = 2/131 (1%)
Query: 10 ASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAAR 69
+ +L+I G IG E+ KN+ ++G + ++D + +L QF K+ + + + A
Sbjct: 14 SKILVIGAGGIGCELLKNLAVTGFRKVHVIDLDTIDISNLNRQFLFRKEHVSSSKAATAT 73
Query: 70 RHIEDMNPRVKLTVDISDLQSK--NKEFFSQFNLIVITDLFPADIEKLNEVTRELNVPIY 127
+ ++ P+++LT D + K N EFF +++++ A +N + N P+
Sbjct: 74 QVVKQFCPQIELTFDHDSIFEKKYNMEFFQAYDIVLNALDNRAARNYVNRMCHAANRPLI 133
Query: 128 VAGINGLSGYI 138
+G G G +
Sbjct: 134 DSGSGGYFGQV 144
>SPAC24H6.12c [O] KOG2015 NEDD8-activating complex catalytic component UBA3
Length = 444
Score = 53.9 bits (128), Expect = 3e-07
Identities = 28/95 (29%), Positives = 47/95 (49%)
Query: 9 SASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAA 68
S+ +L+I G +G EI K++ LSG L+++D + +L QF + +I + A
Sbjct: 44 SSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNIDEPKANVA 103
Query: 69 RRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIV 103
I P +T +Q K EF+ +F LI+
Sbjct: 104 ASMIMKRIPSTVVTPFYGKIQDKTIEFYKEFKLII 138
>CE25130 [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 456
Score = 53.5 bits (127), Expect = 4e-07
Identities = 30/127 (23%), Positives = 61/127 (47%), Gaps = 2/127 (1%)
Query: 14 LINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAARRHIE 73
+I G IG E+ KN+ ++G + ++D + +L QF K+ + + + A + ++
Sbjct: 36 VIGAGGIGCELLKNLAVTGFRKVHVIDLDTIDISNLNRQFLFRKEHVSSSKAATATQVVK 95
Query: 74 DMNPRVKLTVDISDLQSK--NKEFFSQFNLIVITDLFPADIEKLNEVTRELNVPIYVAGI 131
P+++LT D + K N EFF +++++ A +N + N P+ +G
Sbjct: 96 QFCPQIELTFDHDSIFEKKYNMEFFQAYDIVLNALDNRAARNYVNRMCHAANRPLIDSGS 155
Query: 132 NGLSGYI 138
G G +
Sbjct: 156 GGYFGQV 162
>YPR066w [O] KOG2015 NEDD8-activating complex catalytic component UBA3
Length = 299
Score = 52.8 bits (125), Expect = 7e-07
Identities = 36/138 (26%), Positives = 67/138 (48%), Gaps = 9/138 (6%)
Query: 12 VLLINLGAIGNEITKNI-VLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLEAARR 70
+L++ G +G EI KN+ +LS + + I+D + +L QF DIG + + A +
Sbjct: 5 ILVLGAGGLGCEILKNLTMLSFVKQVHIVDIDTIELTNLNRQFLFCDKDIGKPKAQVAAQ 64
Query: 71 HIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIV--ITDLFPADI--EKLNEVTRELN--- 123
++ P++++ + DL + F+ F I+ + + P E L ++T E N
Sbjct: 65 YVNTRFPQLEVVAHVQDLTTLPPSFYKDFQFIISGLDAIEPRRFINETLVKLTLESNYEI 124
Query: 124 -VPIYVAGINGLSGYIFT 140
+P G GL G++ T
Sbjct: 125 CIPFIDGGTEGLKGHVKT 142
>ECU02g1340 [O] KOG2013 SMT3/SUMO-activating complex catalytic component UBA2
Length = 429
Score = 50.8 bits (120), Expect = 3e-06
Identities = 26/96 (27%), Positives = 47/96 (48%)
Query: 7 MRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLE 66
M +L++ G IG E+ K + + S+T++DS V +L QFF +DDIG +
Sbjct: 1 MTDGRILVVGCGGIGCELLKLLAREKLESITLVDSDTVDLSNLNRQFFFNRDDIGKSKAT 60
Query: 67 AARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLI 102
A + +N R + +D+ + FF+ + +
Sbjct: 61 VAAGIFKKLNERCDVFPICADITEFDARFFAGYRTV 96
>CE28320 [H] KOG2017 Molybdopterin synthase sulfurylase
Length = 402
Score = 50.1 bits (118), Expect = 4e-06
Identities = 29/127 (22%), Positives = 65/127 (50%), Gaps = 1/127 (0%)
Query: 1 MTAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDI 60
++ Q N+++ +VL++ G +G + + +GIG++ I+D ++ ++L Q +D +
Sbjct: 29 VSGQKNLKNLNVLIVGAGGLGCPVATYLGAAGIGTIGIVDYDHISLDNLHRQVAYKEDQV 88
Query: 61 GTKRLEAARRHIEDMNPRVKLTVDISDLQSKN-KEFFSQFNLIVITDLFPADIEKLNEVT 119
G + +A +I+ N + + V + L S N + F + ++ A +N+V
Sbjct: 89 GKSKAQALADNIKLQNSDLNVQVHNTSLDSSNAMQLFKNYEIVCDCTDNVATRYLINDVC 148
Query: 120 RELNVPI 126
LN+P+
Sbjct: 149 VLLNIPL 155
>SPAC2G11.10c [H] KOG2017 Molybdopterin synthase sulfurylase
Length = 401
Score = 49.3 bits (116), Expect = 7e-06
Identities = 34/123 (27%), Positives = 61/123 (48%), Gaps = 9/123 (7%)
Query: 1 MTAQANMRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDI 60
+ Q +++ +SVL+I G +G + +V +GIG+L I+D V + +L Q
Sbjct: 35 LPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSNLHRQIIHSTSKQ 94
Query: 61 GTKRLEAARRHIEDMNPRVKLTVDISDLQSKNKEFFSQFNLIVITDLFPADIEKL-NEVT 119
G + +A++ +ED+NP V + + EF S NL I + + ++ N+ T
Sbjct: 95 GMHKAISAKQFLEDLNPNVIINTYL--------EFASASNLFSIIEQYDVVLDCTDNQYT 146
Query: 120 REL 122
R L
Sbjct: 147 RYL 149
>CE04374 [O] KOG2015 NEDD8-activating complex catalytic component UBA3
Length = 437
Score = 48.9 bits (115), Expect = 1e-05
Identities = 26/112 (23%), Positives = 56/112 (49%), Gaps = 8/112 (7%)
Query: 7 MRSASVLLINLGAIGNEITKNIVLSGIGSLTILDSHDVTEEDLGAQFFIGKDDIGTKRLE 66
+++ +L+I G +G E+ KN+ LSG ++ ++D + +L QF + D+G + E
Sbjct: 40 LQNTKILVIGAGGLGCELLKNLALSGFRTIEVIDMDTIDVSNLNRQFLFRESDVGKSKAE 99
Query: 67 AARRHI-------EDMNPRVKLTVDISDLQSKNKEFFSQFNLIVI-TDLFPA 110
A + ++ + + ++ K +EF+ +F++I+ D PA
Sbjct: 100 VAAAFVQQRVVGCQNYFNFISIFRHNCRIEDKGQEFYRKFSIIICGLDSIPA 151
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,329,224
Number of Sequences: 60738
Number of extensions: 719996
Number of successful extensions: 2465
Number of sequences better than 1.0e-05: 41
Number of HSP's better than 0.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 2375
Number of HSP's gapped (non-prelim): 73
length of query: 319
length of database: 30,389,216
effective HSP length: 106
effective length of query: 213
effective length of database: 23,950,988
effective search space: 5101560444
effective search space used: 5101560444
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)