ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV1250    suspect: LH   O     KOG4614  Posttranslational modification, protein turnover, chaperones   Inner membrane protein required for assembly of the F0 sector of ATP synthase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV1250 440402 439554 -283 
         (283 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YLR393w [O] KOG4614 Inner membrane protein required for assembly...   240   1e-63
SPAC4G8.11c [O] KOG4614 Inner membrane protein required for asse...    60   5e-09

>YLR393w [O] KOG4614 Inner membrane protein required for assembly of the F0
           sector of ATP synthase
          Length = 279

 Score =  240 bits (613), Expect = 1e-63
 Identities = 120/251 (47%), Positives = 171/251 (67%), Gaps = 6/251 (2%)

Query: 31  SAPRDIKIEELKKPIGLEKPPFNTKEYKYGNSFLDLFNSEKTGKRAEELEVEFRKSGFHD 90
           ++P++ +I++L KPIGL + P  + +Y  GNS  D+F+SEKT  R +EL VEF KSG +D
Sbjct: 30  ASPKEYQIKQLVKPIGLTQAPRKSTKYSQGNSLRDMFDSEKTNHRVKELAVEFSKSGLYD 89

Query: 91  LYVLKKTNGKIFISPPSYWKADKSLYFPHVVGQRLSDNKECNVEDTLKGKVSIIRLFTTD 150
           + V +KT GK+FI+P SYWK DK+L+FPH++G  +   K+ N+ED L+GK SI+RLF+T 
Sbjct: 90  VQVFQKTKGKLFIAPVSYWKEDKALFFPHLIGTAMDGTKQQNIEDMLRGKTSIVRLFSTA 149

Query: 151 VGKNLINSYF---IDNKHNLDYLKNDESLLKVQDGLKSAQIVEINLAENWLKTMVVKLVK 207
            G  L +SYF   +D+    DYL   ++ L + D   + QI+E+NL EN +K+ +VK + 
Sbjct: 150 SGDKLSSSYFQGIVDDNKKTDYLTEADARLSLND--SNVQIIEVNLVENAVKSALVKTLA 207

Query: 208 NRYASIVPPHRHDKTFISNRDQLPFQIREQLQINNLYSGYVFVVDENLKIRWAACGEATQ 267
            R+A+ VP  R    F  +R Q PF +RE+L  NN++SGYVF+VD+ LKIRWAACGEAT 
Sbjct: 208 -RWANRVPSWRQPFYFECSRGQWPFSVREELFCNNVFSGYVFLVDQQLKIRWAACGEATP 266

Query: 268 KDFNLLWKCVK 278
            +   LWK  K
Sbjct: 267 SEKEALWKFAK 277


>SPAC4G8.11c [O] KOG4614 Inner membrane protein required for assembly of the F0
           sector of ATP synthase
          Length = 267

 Score = 59.7 bits (143), Expect = 5e-09
 Identities = 57/238 (23%), Positives = 104/238 (42%), Gaps = 24/238 (10%)

Query: 44  PIGLEKPPFNTKEYKYGNSFLDLFNSEKTG---KRAEELEVEFRKSGFHDLYVLKKTNGK 100
           P+GL   P    E +    +  L     T    +R +EL  E ++S   D   +++ NGK
Sbjct: 43  PVGLLVKPTMLSEVQKPTLWEKLTKPASTSSPEQRQKELLNEMKRSTIQDFNEVRRFNGK 102

Query: 101 IFISPPSYWKADKSLYFPHVVGQRLSDNKECNVEDTLKGKVSIIRLFTTDVGKNLINSYF 160
           +F SPP  +K   +L+  +  G+ L  N+   +    +G      LF+   G+N   ++ 
Sbjct: 103 LFYSPPRLFKEKSALWMYNFHGKSLR-NQYKELYKDWQGSPFFFALFSNAFGENQCRNW- 160

Query: 161 IDNKHNLDYLKNDESLLKVQDGLKSAQIVEINLAENWLKTMVVKLVKNRYASIVPPHRHD 220
           I +    DYL            ++ A  V+ NL + WL+ + +  VK      +P    +
Sbjct: 161 IQHLAPFDYLP-----------VRYAN-VQSNLIKYWLQKIFIGKVKRS----IPEKCWN 204

Query: 221 KTFISNRDQLPFQIREQLQINNLYSGYVFVVDENLKIRWAACGEATQKDFNLLWKCVK 278
           +   +  ++  F    ++  NN   G V+++D++ KIRW   G+ T  +   L   +K
Sbjct: 205 EYITAYDNKFSF---PEIGWNNKLVGNVYLIDDSCKIRWLGTGDPTNVEIENLKTAIK 259


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,741,789
Number of Sequences: 60738
Number of extensions: 762197
Number of successful extensions: 1868
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1864
Number of HSP's gapped (non-prelim): 2
length of query: 283
length of database: 30,389,216
effective HSP length: 105
effective length of query: 178
effective length of database: 24,011,726
effective search space: 4274087228
effective search space used: 4274087228
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)