ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1365 good I KOG4667 Lipid transport and metabolism Predicted esterase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1365 483509 485371 621
(621 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL057w [I] KOG4667 Predicted esterase 262 1e-69
At3g47590 [I] KOG4667 Predicted esterase 55 2e-07
At1g29840 [I] KOG4667 Predicted esterase 54 7e-07
At2g19550 [I] KOG4667 Predicted esterase 53 1e-06
At5g11910 [I] KOG4667 Predicted esterase 51 5e-06
>YDL057w [I] KOG4667 Predicted esterase
Length = 328
Score = 262 bits (670), Expect = 1e-69
Identities = 141/328 (42%), Positives = 200/328 (59%), Gaps = 12/328 (3%)
Query: 4 DKETITVNTVKNPHEQTVPLLENEQFIYIKDQKRHTGVGLAAILSKPPSLQFDTLAERVK 63
+K+ +TV P + L NE+F+YI +AAI+S P T K
Sbjct: 2 EKKHVTVQIQSAPPSY-IKLEANEKFVYITSTMNGLSYQIAAIVSYPEKRNSSTAN---K 57
Query: 64 NEFKFAFPTHKLVLLLHGHQSFKNALYQPLLADKLSKNGYYVLRIDFRGLGDSEDNRVPE 123
+ K +KL LLLHG QS KNA+YQ LLA +L++ GY+VLRIDFRG GDS DN P
Sbjct: 58 EDGKLLCKENKLALLLHGSQSHKNAIYQTLLAKRLAEFGYWVLRIDFRGQGDSSDNCDPG 117
Query: 124 LGRTIQQDVEDLTTIYEFVSSDACKSLLGRALT--LDTIIAHSRGVISMFEF------AR 175
LGRT+ QD+EDL+T+Y+ VS + + L + T LD ++AHSRG ++MF+F A
Sbjct: 118 LGRTLAQDLEDLSTVYQTVSDRSLRVQLYKTSTISLDVVVAHSRGSLAMFKFCLKLHAAE 177
Query: 176 SFYVPNLINCCGRFDSQGLLLKAARRCPQWEEDGGFYCNTLRFGKWQDVWIPRQETLSAG 235
S +LINC GR+D +GL+ + R P W+ +GGF+ N R G+++D WIP ET S
Sbjct: 178 SPLPSHLINCAGRYDGRGLIERCTRLHPHWQAEGGFWANGPRNGEYKDFWIPLSETYSIA 237
Query: 236 TLDTSKFEEINSETWILSVYTATDPVIPIASAAGYSNLFEGRHTLEIVPNCDHNFYGFPD 295
+ +F I ++S Y D ++PI++A+ Y+ LFEGRH+L+++ N DHN+YG
Sbjct: 238 GVCVPEFATIPQTCSVMSCYGMCDHIVPISAASNYARLFEGRHSLKLIENADHNYYGIEG 297
Query: 296 DTNKLNLPIRKGKVNYGAYLVAYLLEYL 323
D N L LPIR+G+VNY +V ++EYL
Sbjct: 298 DPNALGLPIRRGRVNYSPLVVDLIMEYL 325
>At3g47590 [I] KOG4667 Predicted esterase
Length = 309
Score = 55.5 bits (132), Expect = 2e-07
Identities = 54/221 (24%), Positives = 102/221 (45%), Gaps = 22/221 (9%)
Query: 75 LVLLLHGHQSFKNALYQPLLADKLSKNGYYVLRIDFRGLGDSEDNRVPELGRTIQQDVED 134
+V+L HG +S K+ +A + K G R DF G G+SE + + +D
Sbjct: 83 IVVLCHGFRSNKSNQIMNNVAAAIQKEGISAFRFDFSGNGESEGS---FYYGNYNHEADD 139
Query: 135 LTTIYEFVSSDACKSLLGRALTLDTIIAHSRGVISMFEFARSFY-VPNLINCCGRFD-SQ 192
L ++ ++ S+ + + I+ HS+G + +A ++ V N+IN GR+D +
Sbjct: 140 LHSVVQYFSN--------KNRVVPIILGHSKGGDVVLLYASKYHDVRNVINLSGRYDLKK 191
Query: 193 GLLLKAARRCPQWEEDGGFYCNTLRFGKWQDVWIPRQETLSAGTLDTSKFE---EINSET 249
G+ + + + GF + G + + +++L L T E +I+ E
Sbjct: 192 GIRERLGEDFLERIKQQGF----IDVGDGKSGYRVTEKSL-MDRLSTDIHEACLKIDKEC 246
Query: 250 WILSVYTATDPVIPIASAAGYSNLFEGRHTLEIVPNCDHNF 290
+L+V+ + D VIP+ A ++ + H LEIV +H +
Sbjct: 247 RVLTVHGSEDEVIPVEDAKEFAKIIP-NHKLEIVEGANHGY 286
>At1g29840 [I] KOG4667 Predicted esterase
Length = 263
Score = 53.9 bits (128), Expect = 7e-07
Identities = 55/220 (25%), Positives = 102/220 (46%), Gaps = 22/220 (10%)
Query: 74 KLVLLLHGHQSFKNALYQPLLADKLSKNGYYVLRIDFRGLGDSEDNRVPELGRTIQQDVE 133
++V+L HG +S KN +A + K G R DF G G+S+ + + +
Sbjct: 36 EIVVLCHGFRSTKNDQVMKNVAAAIEKEGISAFRFDFSGNGESKGS---FYFGNYNYEAD 92
Query: 134 DLTTIYEFVSSDACKSLLGRALTLDTIIAHSRGVISMFEFARSFY-VPNLINCCGRFD-S 191
DL ++ + ++ + R + + II HS+G + +A + + N+IN GR+D
Sbjct: 93 DLHSVIRYFTN------MNRVVPI--IIGHSKGGDVVLVYASKYQDIRNVINLSGRYDLK 144
Query: 192 QGLLLKAARRCPQWEEDGGFYCNTLRFGKWQDVWIPRQETLSAGTLDTSKFE---EINSE 248
+G+ + + + GF + + + +E+L L+T E +I+ E
Sbjct: 145 RGIGERLGEDYLERIKQQGF----IDIKEGNAGFRVTEESLME-RLNTDMHEACLKIDKE 199
Query: 249 TWILSVYTATDPVIPIASAAGYSNLFEGRHTLEIVPNCDH 288
+L+V+ + D VIP+ A ++ + H LEIV DH
Sbjct: 200 CRVLTVHGSADEVIPLEDAKEFAKIIP-NHKLEIVEGADH 238
>At2g19550 [I] KOG4667 Predicted esterase
Length = 332
Score = 53.1 bits (126), Expect = 1e-06
Identities = 56/222 (25%), Positives = 98/222 (43%), Gaps = 19/222 (8%)
Query: 74 KLVLLLHGHQSFKNALYQPLLADKLSKNGYYVLRIDFRGLGDSEDNRVPELGRTIQQDVE 133
++V+L HG +S K +A L K R DF G GDSE G + +
Sbjct: 27 EVVVLCHGFRSDKTNKILKNVATALEKEKISSFRFDFSGNGDSEGTFY--YGNFNSEAED 84
Query: 134 DLTTIYEFVSSDACKSLLGRALTLDTIIAHSRGVISMFEFARSF--YVPNLINCCGRFDS 191
DL + + +SS + L + I+ HS+G + +A F Y+ N++N GRFD
Sbjct: 85 DLHYVIQHLSSSNIMNRL-----VPVILGHSKGGDVVLLYASKFPDYIRNVVNISGRFDL 139
Query: 192 QGLLLKAARRCPQWEEDGGFYCNTLRFGKWQDVWIPRQETLSAGTLDTSKFE---EINSE 248
+ + + +E G + + + + QE+L L+T + I+ +
Sbjct: 140 KNDVRLGDGYIEKIKEQG-----FIDATEGKSCFRVTQESL-MDRLNTDMHQACLNIDKQ 193
Query: 249 TWILSVYTATDPVIPIASAAGYSNLFEGRHTLEIVPNCDHNF 290
+L+V+ + D V+P A ++ + H LEIV +H +
Sbjct: 194 CKVLTVHGSDDTVVPGEDAKEFAKVIP-NHKLEIVEGANHGY 234
>At5g11910 [I] KOG4667 Predicted esterase
Length = 339
Score = 51.2 bits (121), Expect = 5e-06
Identities = 53/220 (24%), Positives = 102/220 (46%), Gaps = 20/220 (9%)
Query: 76 VLLLHGHQSFKNALYQPLLADKLSKNGYYVLRIDFRGLGDSEDNRVPELGRTIQQDVEDL 135
V++ HG +S KN + +A + R DF G G+S+ + + G +++VEDL
Sbjct: 87 VVICHGFRSSKNRIPMLTIASFFERAMISSFRFDFAGNGESQGSF--QYG-NYRREVEDL 143
Query: 136 TTIYEFVSSDACKSLLGRALTLDTIIAHSRGVISMFEFARSFY-VPNLINCCGRFD-SQG 193
++ + L G + II HS+G + +A + V ++N GRF +G
Sbjct: 144 RSVLQ--------HLRGVNRVISAIIGHSKGGNVVLLYAAKYNDVQTVVNISGRFFLDRG 195
Query: 194 LLLKAARRCPQWEEDGGFYCNTLRFGKWQDVWIPRQETLSAGTLDTSKFE---EINSETW 250
+ + + + +D GF + R GK++ + +E+L L T+ E I
Sbjct: 196 IEFRLGKDYFKRIKDNGFIDVSNRKGKFE--YRVTEESL-MDRLTTNAHEACLSIRENCR 252
Query: 251 ILSVYTATDPVIPIASAAGYSNLFEGRHTLEIVPNCDHNF 290
+L+V+ + D ++ + A+ ++ + H L ++ DH F
Sbjct: 253 VLTVHGSNDRIVHVTEASEFAKQIK-NHKLYVIEGADHEF 291
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.138 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,770,576
Number of Sequences: 60738
Number of extensions: 1665724
Number of successful extensions: 3395
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3391
Number of HSP's gapped (non-prelim): 5
length of query: 621
length of database: 30,389,216
effective HSP length: 112
effective length of query: 509
effective length of database: 23,586,560
effective search space: 12005559040
effective search space used: 12005559040
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)