ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1437 good E KOG2275 Amino acid transport and metabolism Aminoacylase ACY1 and related metalloexopeptidases
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1437 505592 503862 -577
(577 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL172w [E] KOG2275 Aminoacylase ACY1 and related metalloexopept... 373 e-103
SPAC24C9.08 [E] KOG2275 Aminoacylase ACY1 and related metalloexo... 257 4e-68
Hs22044045 [E] KOG2275 Aminoacylase ACY1 and related metalloexop... 110 4e-24
7300958 [E] KOG2275 Aminoacylase ACY1 and related metalloexopept... 65 2e-10
At4g38220 [E] KOG2275 Aminoacylase ACY1 and related metalloexope... 65 4e-10
7300956 [E] KOG2275 Aminoacylase ACY1 and related metalloexopept... 64 5e-10
7300957 [E] KOG2275 Aminoacylase ACY1 and related metalloexopept... 63 1e-09
7300959 [E] KOG2275 Aminoacylase ACY1 and related metalloexopept... 62 2e-09
7300955 [E] KOG2275 Aminoacylase ACY1 and related metalloexopept... 62 2e-09
At1g44180 [E] KOG2275 Aminoacylase ACY1 and related metalloexope... 60 7e-09
At1g44820 [E] KOG2275 Aminoacylase ACY1 and related metalloexope... 58 3e-08
>YJL172w [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 576
Score = 373 bits (957), Expect = e-103
Identities = 208/522 (39%), Positives = 300/522 (56%), Gaps = 16/522 (3%)
Query: 52 QCQTYDKVSGGSTDDLNYIISDESFINGTIGKLQNIIRIPTEVSDERPDPGTHPDEDMYK 111
+C+ + +S ++ I+ D +F N +I KL N +RIPT V D+ P+P PD YK
Sbjct: 62 KCEKIEPLSPSFKHSVDTILHDPAFRNSSIEKLSNAVRIPTVVQDKNPNPADDPD--FYK 119
Query: 112 PFYELHKQLEHDFPLVWSKLQVEIVNNLALLISWKGTDESLKPLMFASHMDVVPVERKTW 171
FYELH E FP + L++E VN L LL +W+G+D LKPL+ +H DVVPV +T
Sbjct: 120 HFYELHDYFEKTFPNIHKHLKLEKVNELGLLYTWEGSDPDLKPLLLMAHQDVVPVNNETL 179
Query: 172 DEWRHPPFSGNIEFDSDNILNSKIWGRGSFDDKNMXXXXXXXXXXXXSQDFQPERGIVLA 231
W+ PPFSG+ + ++D + WGRGS D KN+ F+P R IV++
Sbjct: 180 SSWKFPPFSGHYDPETDFV-----WGRGSNDCKNLLIAEFEAIEQLLIDGFKPNRTIVMS 234
Query: 232 VGSDEEASGQFGAAKINEILMERYGDDGIYAIVDEGLNGIKKQEGVYMASPGTAEKGFIN 291
+G DEEASG GAA + L ERYGDDGIY+I+DEG ++ + V++A+P AEKG+++
Sbjct: 235 LGFDEEASGTLGAASLASFLHERYGDDGIYSIIDEGEGIMEVDKDVFVATPINAEKGYVD 294
Query: 292 FWIHLTTPGGHSSVPPDHTSIGIISSLVKKIESEKFPLWFTEKNPVSQWHQCAAQYSLEM 351
F + + GGHSSVPPDHT+IGI S L+ + E+ F F NP+ CAA++S +
Sbjct: 295 FEVSILGHGGHSSVPPDHTTIGIASELITEFEANPFDYEFEFDNPIYGLLTCAAEHSKSL 354
Query: 352 DNSLRYDFLNAMNDEGSNARVIEYLLQTGGRSMEYLLRTSQAVDIIHGGFKSNALPETVS 411
++ L A +++EY+ + + L+RT+QAVDII+GG K+NALPET
Sbjct: 355 SKDVKKTILGAPFCPRRKDKLVEYI--SNQSHLRSLIRTTQAVDIINGGVKANALPETTR 412
Query: 412 VLINSRIALESTVNETMTKFINQLKEISNENDLGLEYNGDELIKP-TEHGTFFVEVVVG- 469
LIN RI L S+V E + I K+I+ + GL NGD+ I P TE G + ++
Sbjct: 413 FLINHRINLHSSVAEVFERNIEYAKKIAEKYGYGLSKNGDDYIIPETELGHIDITLLREL 472
Query: 470 KXXXXXXXXXXXWKHFAGTVKSFYEDIVFPTKLEEDSTELVIAPTIMSGNTDCLHYLSLT 529
+ W AGT++ +E+ V +++ E + + SGNTD +Y +L+
Sbjct: 473 EPAPLSPSSGPVWDILAGTIQDVFENGVL-----QNNEEFYVTTGLFSGNTDTKYYWNLS 527
Query: 530 ENIYRFQPGFASKDTLGTIHSVNEHVDFETVMHTVAFVYHYI 571
+NIYRF D L T+HSVNEHVD + +AFVY YI
Sbjct: 528 KNIYRFVGSIIDIDLLKTLHSVNEHVDVPGHLSAIAFVYEYI 569
>SPAC24C9.08 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 596
Score = 257 bits (656), Expect = 4e-68
Identities = 164/518 (31%), Positives = 264/518 (50%), Gaps = 39/518 (7%)
Query: 61 GGSTDDLNYIISDESFINGTIGKLQNIIRIPTEVSDERPDPGTHPDEDMYKPFYELHKQL 120
G ++YI S E F N ++ + Q ++RIPT D+ D G D+D + F ++
Sbjct: 102 GDDVSGIDYIRSPE-FFNDSLVRFQELLRIPTVCYDDMGDVG---DDDRFDIFAVFQDKV 157
Query: 121 EHDFPLVWSKLQVEIVNNLALLISWKGTDESLKPLMFASHMDVVPVERKTWDEWRHPPFS 180
+P ++ KL+VE VN LLI+ +G+++ LKPL+ H DVVPV + + D W PPFS
Sbjct: 158 RELYPNIFKKLKVEYVNTYGLLITLEGSNKDLKPLVLMGHQDVVPVNQASLDRWYFPPFS 217
Query: 181 GNIEFDSDNILNSKIWGRGSFDDKNMXXXXXXXXXXXXSQDFQPERGIVLAVGSDEEASG 240
N ++ RG+ DDKN D++PE+ ++ + G DEE SG
Sbjct: 218 ATYH-------NGHVYSRGAADDKNSVVAILEALEILAISDYKPEQTVIASFGFDEEVSG 270
Query: 241 QFGAAKINEILMERYGDDGIYAIVDEGLNGIKKQEGVYMASPGTAEKGFINFWIHLTTPG 300
GA + L ERYG DG+ I+DEG I G A+ AEKG+++ + L TPG
Sbjct: 271 YRGALPLAHKLYERYGKDGVALILDEGGFTI-NLFGTLFATVCVAEKGYMDVHLKLKTPG 329
Query: 301 GHSSVPPDHTSIGIISSLVKKIESEKFPLWFTEKNPVSQWHQCAAQYSLEMDNSLRYDFL 360
GH+S+PP HT+IG++S LV +IE E F T +NP QC A+ S +MD++LR
Sbjct: 330 GHASIPPPHTNIGLMSKLVTQIE-EPFGGELTFENPFYTTLQCFAENSADMDDNLR---- 384
Query: 361 NAMNDEGSNARVIEYLLQTGGRSMEYLLRTSQAVDIIHGGFKSNALPETVSVLINSRIAL 420
+ G ++ + L + R Y TS AVD+I+GG K NALPE ++ +N R+
Sbjct: 385 -QLIKSGDTEKMTD--LFSKSRLYRYFFETSIAVDVINGGVKVNALPEETTLAVNHRVDA 441
Query: 421 ESTVNETMTKFINQLKEISNENDLGLE-YNGDELIKPTEH-GTFF---VEVVVGKXXXXX 475
+ + ++ L+E +E + + +NG+ +++ + G F + +
Sbjct: 442 SKGLKQVYDRYGGLLEEFGHEYHVNVTLFNGETVVEYEDAIGHIFASTAKTLEPSPVSPY 501
Query: 476 XXXXXXWKHFAGTVKSFYEDIVFPTKLEEDSTELVIAPTIMSGNTDCLHYLSLTENIYRF 535
+K AG ++ + D + P +M NTD HY +LT NIYR+
Sbjct: 502 DESSDAYKKLAGAIRYTFGD------------GTSVTPALMPANTDTRHYWNLTSNIYRW 549
Query: 536 QPGFASKDTLGTI--HSVNEHVDFETVMHTVAFVYHYI 571
P + + + H++NE++ ++ M ++ F Y++I
Sbjct: 550 TPVSTNSTSKNSFNGHTINENMRYDAHMDSIEFFYNFI 587
>Hs22044045 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 361
Score = 110 bits (276), Expect = 4e-24
Identities = 78/238 (32%), Positives = 119/238 (49%), Gaps = 24/238 (10%)
Query: 115 ELHKQLEHDFPLVWSK--LQVEIVNNLALLISWKGTDESLKPLMFASHMDVVPVERKTWD 172
E K + FP V S +Q E+V + L + +G+D SL+P + +H DVVP + W+
Sbjct: 78 EFGKYIHKVFPTVVSTSFIQHEVVEEYSHLFTIQGSDPSLQPYLLMAHFDVVPAPEEGWE 137
Query: 173 EWRHPPFSGNIEFDSDNILNSKIWGRGSFDDKNMXXXXXXXXXXXXSQDFQPERGIVLAV 232
PPFSG + D I I+GRG+ DDKN + + P R +++
Sbjct: 138 V---PPFSG---LERDGI----IYGRGTLDDKNSVMALLQALELLLIRKYIPRRSFFISL 187
Query: 233 GSDEEASGQFGAAKINEILMER------YGDDGIYAIVDEGLNGIKKQEGVYMASPGTAE 286
G DEE+SG GA +I+ +L R D+G + I+D+ + KK +A +E
Sbjct: 188 GHDEESSGT-GAQRISALLQSRGVQLAFIVDEGGF-ILDDFIPNFKKP----IALIAVSE 241
Query: 287 KGFINFWIHLTTPGGHSSVPPDHTSIGIISSLVKKIESEKFPLWFTEKNPVSQWHQCA 344
KG +N + + GHSS PP TSIGI+++ V ++E P+ F V+ Q A
Sbjct: 242 KGSMNLMLQVNMTSGHSSAPPKETSIGILAAAVSRLEQTPMPIIFGSGTVVTVLQQLA 299
>7300958 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 401
Score = 65.5 bits (158), Expect = 2e-10
Identities = 41/146 (28%), Positives = 66/146 (45%), Gaps = 10/146 (6%)
Query: 101 PGTHPDEDMYKPFYELHKQLEHDFPLVWSKLQVEIVNNLALLISWKGTDESLKPLMFASH 160
P HPD D Y E K+ L + + ++I W+G+ L ++ SH
Sbjct: 22 PTVHPDVD-YTACVEFLKRQASSLNLPVEVVYPAVQTKPVVIIKWEGSQPELSSIVLNSH 80
Query: 161 MDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGSFDDKNMXXXXXXXXXXXXSQ 220
DVVPV R ++W H PFS +I+ + +I+ RG+ D K++ +
Sbjct: 81 TDVVPVFR---EKWTHEPFSADIDEE------GRIFARGTQDMKSVGTQYLGAIRLLKAS 131
Query: 221 DFQPERGIVLAVGSDEEASGQFGAAK 246
F+P+R + + DEE G G A+
Sbjct: 132 GFKPKRNLYVTFVPDEETGGHLGMAE 157
>At4g38220 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 753
Score = 64.7 bits (156), Expect = 4e-10
Identities = 63/247 (25%), Positives = 101/247 (40%), Gaps = 37/247 (14%)
Query: 81 IGKLQNIIRIPTEVSDERPDPGTHPDEDMYKPFYELHKQLEHDFPLVWSKLQVEIVNNLA 140
+ + Q +RI T P+ + YK + Q + PL +E V
Sbjct: 25 VSRFQEYLRINT----------VQPNPEYYKAVDFIISQAK---PLSLESQTIEFVKGKP 71
Query: 141 LLI-SWKGTDESLKPLMFASHMDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRG 199
LL+ W G+D +L + SH DVVP E +W H P +++ D I+ RG
Sbjct: 72 LLLLKWVGSDPTLPAFLLNSHTDVVPFED---SKWTHHPLQAHMDHHGD------IYARG 122
Query: 200 SFDDKNMXXXXXXXXXXXXSQDFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDG 259
S D K + + F+P R + L+ DEE G GA K E + +
Sbjct: 123 SQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAE--SQLFKSLN 180
Query: 260 IYAIVDEGLNGIKKQEGVYMASPGTAEKGFINFWIHLTT--PGGHSSVPPDHTSIGIISS 317
I ++DEGL + V+ +W+ + P GH + D+++ + +
Sbjct: 181 IAIVLDEGLPSPTESYRVFYGERSP-------WWLVIKAKGPPGHGAKLYDNSA---MEN 230
Query: 318 LVKKIES 324
L+K IES
Sbjct: 231 LLKSIES 237
>7300956 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 408
Score = 64.3 bits (155), Expect = 5e-10
Identities = 80/366 (21%), Positives = 134/366 (35%), Gaps = 86/366 (23%)
Query: 101 PGTHPDEDMYKPFYELHKQLEHDFPLVWSKLQVEIVNNLA-------------------L 141
P HPD D K + + L+ P+ + VE + A +
Sbjct: 24 PSVHPDVDYSKDLF--FRSLDFVIPISLAAACVEFLKRQANKLNLRVDVVYPVVPSKPVV 81
Query: 142 LISWKGTDESLKPLMFASHMDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGSF 201
++ W G LK ++ SHMDVVPV ++W H PF +I+ +I+ RG+
Sbjct: 82 IMKWLGKHPELKSIILNSHMDVVPVFP---EKWTHEPFGAHID------AQGRIYARGAQ 132
Query: 202 DDKNMXXXXXXXXXXXXSQDFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDGIY 261
D K++ + +QP+R + L DEE G G A+ + + + +
Sbjct: 133 DMKSVGCQYMAAVRALKASGYQPKRTVYLTFVPDEETGGHMGMAEF--VKGDYFKAMNVG 190
Query: 262 AIVDEGLNGIKKQEGVYMASPGTAEKGFINFWIHLTTPGGHSSVPPDHTSIGIISSLVKK 321
+DE GI ++ Y AE+ + GH S+ L K
Sbjct: 191 FSLDE---GIASEDDTYPVF--YAERTLWQLRFKFSGTSGHGSL------------LHKS 233
Query: 322 IESEKFPLWFTEKNPVSQWHQCAAQYSLEMDNSLRYDFLNAMNDEGSNARVIEYLLQTGG 381
EKF MD +++ R + L
Sbjct: 234 TAGEKF--------------------HFVMDKLMKF-------------RETQVKLLAED 260
Query: 382 RSMEYLLRTSQAVDIIHGGFKSNALPETVSVLINSRIALESTVNETMTKFINQLKEISNE 441
S++ T+ + ++GG +SN +P + + RIA +N+ NQ++E NE
Sbjct: 261 SSLQSGDVTTLNLTQLNGGVQSNVVPPVLEATFDIRIA----INQNADAMENQIREWCNE 316
Query: 442 NDLGLE 447
G+E
Sbjct: 317 VGGGVE 322
>7300957 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 400
Score = 63.2 bits (152), Expect = 1e-09
Identities = 38/127 (29%), Positives = 62/127 (47%), Gaps = 11/127 (8%)
Query: 141 LLISWKGTDESLKPLMFASHMDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGS 200
++I W GT+ L ++ SHMDVVPV R D+W H PF+ +I+ + +I+ RG+
Sbjct: 60 VIIKWLGTEPELPSVILNSHMDVVPVFR---DKWTHDPFAADIDEE------GRIFARGT 110
Query: 201 FDDKNMXXXXXXXXXXXXSQDFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDGI 260
D K++ + QP+R + DEE G+ G + + E Y + +
Sbjct: 111 QDMKSVGTGYLGAIRLLKASGVQPKRNFFVTFVPDEEIGGELGMQEF--VKTEYYSNMNV 168
Query: 261 YAIVDEG 267
+DEG
Sbjct: 169 GFSLDEG 175
>7300959 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 340
Score = 62.0 bits (149), Expect = 2e-09
Identities = 46/167 (27%), Positives = 74/167 (43%), Gaps = 12/167 (7%)
Query: 101 PGTHPDEDMYKPFYELHKQLEHDFPLVWSKLQVEIVNNLALLISWKGTDESLKPLMFASH 160
P HP+ D Y E K+ L + + + ++I W+G+ L ++ +SH
Sbjct: 22 PSVHPNID-YTACVEFIKRQADSLNLPVEVVFPAVKSKPVVIIKWEGSQPKLPSIILSSH 80
Query: 161 MDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGSFDDKNMXXXXXXXXXXXXSQ 220
MDVVPV + W H PFS +I+ + +I+ RG+ D K++ +
Sbjct: 81 MDVVPVFP---EMWTHEPFSADIDEE------GRIFARGAQDMKSVGTQYLGAIRLLRAD 131
Query: 221 DFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDGIYAIVDEG 267
FQP+R + + DEE G G A E + Y + +DEG
Sbjct: 132 GFQPKRTLYVTFVPDEEIGGIHGMAAFVE--TDFYKQMNVGFSLDEG 176
>7300955 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 359
Score = 62.0 bits (149), Expect = 2e-09
Identities = 52/222 (23%), Positives = 95/222 (42%), Gaps = 17/222 (7%)
Query: 101 PGTHPDEDMYKPFYELHKQLEHDFPLVWSKLQVEIVNNLALLISWKGTDESLKPLMFASH 160
P HP+ D Y E K+ L + N +++ W+G+ L ++ SH
Sbjct: 22 PTVHPNVD-YTVCTEFLKRQAASLDLPVEVIYPVNEQNPVVVLKWQGSQPELPSIILNSH 80
Query: 161 MDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGSFDDKNMXXXXXXXXXXXXSQ 220
DVVPV ++W H PFS +++ + +I+ RGS D K + +
Sbjct: 81 TDVVPVFE---EKWTHGPFSADLDAE------GRIFARGSQDMKCVGTQYLGAVRALKAS 131
Query: 221 DFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDGIYAIVDEGLNGIKKQEGVYMA 280
+QP+R I L DEE G G ++ + + + + DEG++ + +Y
Sbjct: 132 GYQPKRTIYLTYVPDEEVGGHLGMREL--VKSDYFKKLNVGFSFDEGISSADETYALYY- 188
Query: 281 SPGTAEKGFINFWIHLTTPGGHSSVPPDHTSIGIISSLVKKI 322
AE+ + ++ GH S+ +T+ ++ +V K+
Sbjct: 189 ----AERTLWHLRFKISGTAGHGSLLLPNTAGEKLNYVVGKM 226
>At1g44180 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 435
Score = 60.5 bits (145), Expect = 7e-09
Identities = 38/127 (29%), Positives = 62/127 (47%), Gaps = 11/127 (8%)
Query: 141 LLISWKGTDESLKPLMFASHMDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGS 200
LL++W G++ +L ++F SH+D VP E ++W HPPFS + ++ I+ RG+
Sbjct: 80 LLLTWLGSNLNLSSILFNSHLDSVPAES---EKWIHPPFSAH------RTIDGHIYARGA 130
Query: 201 FDDKNMXXXXXXXXXXXXSQDFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDGI 260
DDK + S+ F P R I ++ +EE G G K + D +
Sbjct: 131 QDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKF--AASSEFKDLNL 188
Query: 261 YAIVDEG 267
++DEG
Sbjct: 189 GFVMDEG 195
>At1g44820 [E] KOG2275 Aminoacylase ACY1 and related metalloexopeptidases
Length = 441
Score = 58.2 bits (139), Expect = 3e-08
Identities = 38/127 (29%), Positives = 61/127 (47%), Gaps = 11/127 (8%)
Query: 141 LLISWKGTDESLKPLMFASHMDVVPVERKTWDEWRHPPFSGNIEFDSDNILNSKIWGRGS 200
LLI+W G++ +L ++F SH+D VP E ++W +PPFS + ++ I+ RG+
Sbjct: 78 LLITWLGSNPNLPSILFNSHLDSVPAES---EKWTYPPFSAH------KTIDGHIYARGA 128
Query: 201 FDDKNMXXXXXXXXXXXXSQDFQPERGIVLAVGSDEEASGQFGAAKINEILMERYGDDGI 260
DDK + S+ F P R I ++ +EE G G K + D +
Sbjct: 129 QDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKF--AASSEFKDLNL 186
Query: 261 YAIVDEG 267
+DEG
Sbjct: 187 GFAMDEG 193
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,755,794
Number of Sequences: 60738
Number of extensions: 1574217
Number of successful extensions: 3354
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 3321
Number of HSP's gapped (non-prelim): 15
length of query: 577
length of database: 30,389,216
effective HSP length: 112
effective length of query: 465
effective length of database: 23,586,560
effective search space: 10967750400
effective search space used: 10967750400
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)