ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1600 good C KOG2759 Energy production and conversion Vacuolar H+-ATPase V1 sector, subunit H
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1600 564268 562889 -460
(460 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR036w [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 355 6e-98
SPAC7D4.10 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 112 2e-24
CE04638 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 107 4e-23
Hs7706262 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 100 5e-21
CE07497 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 90 8e-18
7298328 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 84 3e-16
7298327 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 74 6e-13
At3g42050 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H 71 3e-12
>YPR036w [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 478
Score = 355 bits (912), Expect = 6e-98
Identities = 202/478 (42%), Positives = 293/478 (61%), Gaps = 30/478 (6%)
Query: 8 ILLDSTHFQRIRHALNSRTIGWDALVRSSEISKFDGETTKSLESRLLKNQKLDLPLDT-- 65
IL+DSTHF IR + SR++ WDAL RS E+S+ D T K+LES L+K D +
Sbjct: 6 ILMDSTHFNEIRSIIRSRSVAWDALARSEELSEIDASTAKALESILVKKNIGDGLSSSNN 65
Query: 66 ----------ALTSLLHILETSNNLDVNKCVVNLFSQLLTLEPYNEQLVELLSKRGARDV 115
L L+H+L TS+N D K V NL ++LL+ + Y + V+ + +
Sbjct: 66 AHSGFKVNGKTLIPLIHLLSTSDNEDCKKSVQNLIAELLSSDKYGDDTVKFFQEDPKQ-- 123
Query: 116 LNQLYEHTLNKSFSDEQFNLISAFTLVSLLLQ--FNDTDLASKLLENKQFQSLFKS-EHK 172
L QL++ +L F Q LIS F +VSLL+Q ++ L KLL+N ++ ++ E
Sbjct: 124 LEQLFDVSLKGDF---QTVLISGFNVVSLLVQNGLHNVKLVEKLLKNNNLINILQNIEQM 180
Query: 173 DSLYLAIRLLQELTVTKAYKQLVWSQEAFYLPIVFQSIF----SXXXXXXXXXXXXXXXX 228
D+ Y+ IRLLQEL V Y+ ++W E ++P +F+ + S
Sbjct: 181 DTCYVCIRLLQELAVIPEYRDVIWLHEKKFMPTLFKILQRATDSQLATRIVATNSNHLGI 240
Query: 229 XXXYYALMIIWLLTFDPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINE 288
Y++L++IWLLTF+P +++L+ +L LL+L++ TIKEKV RL ++IIL +
Sbjct: 241 QLQYHSLLLIWLLTFNPVFANELVQKYLSDFLDLLKLVKITIKEKVSRLCISIILQCCST 300
Query: 289 SVKNHKSTVKNLILLGNAIPTLNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYL 348
VK HK +K L+LLGNA+PT+ + ERK+ D+EL+QD++ LKE LE EY ELTSFDEY+
Sbjct: 301 RVKQHKKVIKQLLLLGNALPTVQSLSERKYSDEELRQDISNLKEILENEYQELTSFDEYV 360
Query: 349 AELNSKILLWSPVHQDDQFWLDNLDKFKENNWKLFLQLIDLLKEFI----TEKRPSSVSL 404
AEL+SK+L WSP H D+ FW DN+D+FK++N+K+F QLI+LL+ + + + +
Sbjct: 361 AELDSKLLCWSPPHVDNGFWSDNIDEFKKDNYKIFRQLIELLQAKVRNGDVNAKQEKIII 420
Query: 405 QILLNDIRKVMELDNDSIKILGK--DKLIIMQLLQHSDSKVKYEALKTTQVIVSNTFK 460
Q+ LNDI V+EL +SI +L K K IM+LL HSDS+VKYEALK TQ I+ TFK
Sbjct: 421 QVALNDITHVVELLPESIDVLDKTGGKADIMELLNHSDSRVKYEALKATQAIIGYTFK 478
>SPAC7D4.10 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 450
Score = 112 bits (279), Expect = 2e-24
Identities = 114/460 (24%), Positives = 199/460 (42%), Gaps = 51/460 (11%)
Query: 10 LDSTHFQRIRHALNSRTIGWDALVRSSEISKFDGETTKSLESRLLKNQKLDLPLDTALTS 69
LD++ I + + I W RS + + + + ++L + L DT S
Sbjct: 19 LDNSQVDEIINNVRCVAIPWQGYQRSGSLEENELQEIENLTGKPLSAYVKTAEEDTTAYS 78
Query: 70 --LLHILETSNNLDV-NKCVVNLFSQLLT----LEPYNEQLVELLSKRGARDVLNQLYEH 122
L +L + DV N +V L LL L + + L K E
Sbjct: 79 NLFLKLLSMKDTPDVVNFALVKLADTLLNSNKFLSAFGPAFYDFLEKD----------ES 128
Query: 123 TLNKSFSDEQFNLISAFTLVSLLLQFNDTDLASKLLENKQFQSLFKSEHKDSLYLAIRLL 182
+N D + F L S + + LE L +S + + A++ L
Sbjct: 129 YINYLDDDSKLLFARVFALCSSSSPCSVAKAFTLFLE--YLGKLMQSLNPLTRLFAVQCL 186
Query: 183 QELTVTKAYKQLVWSQE--AFYLPIVFQSIFSXXXXXXXXXXXXXXXXXXXYYALMIIWL 240
+ KA++ +W++ +F L + ++ YY+L W
Sbjct: 187 NGVLTLKAHRYALWAENTCSFRLAELLRNSIGDTQLQ--------------YYSLFCFWQ 232
Query: 241 LTFDPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINESVKNHKSTVKNL 300
LTF+ I + K L L+++IRS K KV RL +AI++N I+++ K+ ST+
Sbjct: 233 LTFESHIAQDINKRFDLIKL-LVQIIRSDTKTKVYRLVLAILVNLIDKAPKDTISTM--- 288
Query: 301 ILLGNAIPTLNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYLAELNSKILLWSP 360
LL + + + +RK+ D+++ L + +L+ L++FD Y +EL++ IL WSP
Sbjct: 289 -LLEHVDKAVQLLQKRKWADEDITNYLDFITSTLDESSKHLSTFDMYKSELDTGILHWSP 347
Query: 361 VHQDDQFWLDNLDKFKENNWKLFLQLIDLLKEFITEKRPSSVSLQILLND----IRKVME 416
H+ + FW N + E+N+ L +L I + + SL + +D IR E
Sbjct: 348 SHRSEDFWHQNAKRLNEDNYALLKKLF-----HIVQYNEDNTSLAVACHDLGAYIRSYPE 402
Query: 417 LDNDSIKILGKDKLIIMQLLQHSDSKVKYEALKTTQVIVS 456
+ IK K + IM L+ H D +V++EAL T Q++++
Sbjct: 403 GRSLIIKYGAKQR--IMDLMSHPDPEVRFEALSTVQLLMT 440
>CE04638 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 451
Score = 107 bits (267), Expect = 4e-23
Identities = 105/461 (22%), Positives = 197/461 (41%), Gaps = 40/461 (8%)
Query: 13 THFQRIRHALNSRTIGWDALVRSSEISKFDGE---TTKSLESRLLKNQKLDLPLDTALTS 69
THFQ+ + + W R+ I++ D + T + E+ ++ L + + A+ +
Sbjct: 12 THFQKEADKVRAMKTNWGLFTRTRMIAQSDYDFIVTYQQAENEAERSTVLSVFKEKAVYA 71
Query: 70 LLHILETSNNLDVNKCVVNLFSQLLTLEPYNEQLVE---LLSKRGARDVLNQLYEHTLNK 126
+H++ + D + + L +L + + E +L KR + +
Sbjct: 72 FVHLMSQISKDDYVRYTLTLIDDMLREDVTRTIIFEDVAVLLKRSP-------FSFFMGL 124
Query: 127 SFSDEQFNLISAFTLVSLLLQFNDTDLASKLLE---NKQFQSLFKSEHKDSLYLAIRLLQ 183
+Q+ + F++++ + F + L+ L+ +++ + + D + A+R +Q
Sbjct: 125 LHRQDQYIVHITFSILTKMAVFGNIKLSGDELDYCMGSLKEAMNRGTNNDYIVTAVRCMQ 184
Query: 184 ELTVTKAYKQLVWSQEAFYLPIVFQSIFSXXXXXXXXXXXXXXXXXXXYYALMIIWLLTF 243
L Y+ + F +I Y + +WLLTF
Sbjct: 185 TLFRFDPYR------------VSFVNINGYDSLTHALYSTRKCGFQIQYQIIFCMWLLTF 232
Query: 244 DPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINESVKNHKSTVKNLILL 303
+ E +L + ++ + KEKV+R+ V+ + N I + + L ++
Sbjct: 233 NGHAAEVALSGNLIQTIS--GILGNCQKEKVIRIVVSTLRNLITSNQDVYMKKQAALQMI 290
Query: 304 GNAIPT-LNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYLAELNSKILLWSPVH 362
N IPT L+ + RKF D +L +D+ L+ L+ LTSFDEY EL L WSP H
Sbjct: 291 QNRIPTKLDHLENRKFTDVDLVEDMVYLQTELKKVVQVLTSFDEYENELRQGSLHWSPAH 350
Query: 363 QDDQFWLDNLDKFKENNWKLFLQLIDLLKEFITEKRPSSVSLQILLNDIRK-VMELDNDS 421
+ + FW +N + +N +L L+ +L EK + L + +DI + V
Sbjct: 351 KCEVFWNENAHRLNDNRQELLKLLVAML-----EKSNDPLVLCVAAHDIGEFVRYYPRGK 405
Query: 422 IKI--LGKDKLIIMQLLQHSDSKVKYEALKTTQVIVSNTFK 460
+K+ LG K +M+LL D V+Y AL Q ++ N +K
Sbjct: 406 LKVEQLG-GKEAMMRLLTVKDPNVRYHALLAAQKLMINNWK 445
>Hs7706262 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 483
Score = 100 bits (249), Expect = 5e-21
Identities = 63/230 (27%), Positives = 121/230 (52%), Gaps = 6/230 (2%)
Query: 232 YYALMIIWLLTFDPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINESVK 291
Y + IWLL F P++ E + ++ L +++ ++KEKV R+ +A N + +S +
Sbjct: 241 YQMIFSIWLLAFSPQMCEHLRRYNIIPVLS--DILQESVKEKVTRIILAAFRNFLEKSTE 298
Query: 292 NHKSTVKNLILLG-NAIPTLNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYLAE 350
L ++ + L + ++K+ D+++ +D+ L E L +L+SFDEY +E
Sbjct: 299 RETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSE 358
Query: 351 LNSKILLWSPVHQDDQFWLDNLDKFKENNWKLFLQLIDLLKEFITEKRPSSVSLQILLND 410
L S L WSPVH+ ++FW +N + E N++L L+++ L E + + +V+ +
Sbjct: 359 LKSGRLEWSPVHKSEKFWRENAVRLNEKNYEL-LKILTKLLEVSDDPQVLAVAAHDVGEY 417
Query: 411 IRKVMELDNDSIKILGKDKLIIMQLLQHSDSKVKYEALKTTQVIVSNTFK 460
+R I+ LG K ++M + H D +V+Y AL Q ++ + ++
Sbjct: 418 VRHYPR-GKRVIEQLG-GKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWE 465
>CE07497 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 470
Score = 89.7 bits (221), Expect = 8e-18
Identities = 62/232 (26%), Positives = 114/232 (48%), Gaps = 10/232 (4%)
Query: 232 YYALMIIWLLTFDPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINE-SV 290
Y + +W LTF+ I L L ++ + KEKV+R+ +A +N +++
Sbjct: 228 YQLIFAVWCLTFNADIARKAPSLGLIQALG--DILSESTKEKVIRIILASFVNILSKVDE 285
Query: 291 KNHKSTVKNLILLGNAIPTLNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYLAE 350
+ K ++ + TL + +K+ D +L+ D+ L E L H+L+S+DEY +E
Sbjct: 286 REVKREAALQMVQCKTLKTLELMDAKKYDDPDLEDDVKFLTEELTLSVHDLSSYDEYYSE 345
Query: 351 LNSKILLWSPVHQDDQFWLDNLDKFKENNWKLFLQLIDLLKEFITEKRPSSVSLQILLND 410
+ S L WSPVH+ ++FW +N KF + +++ LI LL E + L + +D
Sbjct: 346 VRSGRLQWSPVHKSEKFWRENASKFNDKQFEVVKILIKLL-----ESSHDPLILCVASHD 400
Query: 411 IRKVME--LDNDSIKILGKDKLIIMQLLQHSDSKVKYEALKTTQVIVSNTFK 460
I + + ++ + K +M+LL D V+Y AL Q ++ + ++
Sbjct: 401 IGEYVRHYPRGKTVVEQYQGKAAVMRLLTAEDPNVRYHALLAVQKLMVHNWE 452
>7298328 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 392
Score = 84.3 bits (207), Expect = 3e-16
Identities = 47/161 (29%), Positives = 85/161 (52%), Gaps = 3/161 (1%)
Query: 232 YYALMIIWLLTFDPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINESVK 291
Y + +W+LTF+P + K + L ++ KEKV R+ +A+ N I +
Sbjct: 207 YQLIFCLWVLTFNPLLAAKMNKFSVIPILA--DILSDCAKEKVTRIILAVFRNLIEKPED 264
Query: 292 NHKSTVKNLILLG-NAIPTLNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYLAE 350
+ + + ++ + L+ + +R+F D+++ D+ L E L+ +L+SFDEY E
Sbjct: 265 SSVAKDHCIAMVQCKVLKQLSILEQRRFDDEDITADVEYLSEKLQNSVQDLSSFDEYATE 324
Query: 351 LNSKILLWSPVHQDDQFWLDNLDKFKENNWKLFLQLIDLLK 391
+ S L WSPVH+ +FW +N + E N++L L+ LL+
Sbjct: 325 VRSGRLEWSPVHKSAKFWRENAQRLNEKNYELLRILVHLLE 365
>7298327 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 356
Score = 73.6 bits (179), Expect = 6e-13
Identities = 39/122 (31%), Positives = 69/122 (55%), Gaps = 1/122 (0%)
Query: 271 KEKVVRLSVAIILNGINESVKNHKSTVKNLILLG-NAIPTLNQIMERKFLDDELKQDLTT 329
KEKV R+ +A+ N I + + + + ++ + L+ + +R+F D+++ D+
Sbjct: 208 KEKVTRIILAVFRNLIEKPEDSSVAKDHCIAMVQCKVLKQLSILEQRRFDDEDITADVEY 267
Query: 330 LKESLEAEYHELTSFDEYLAELNSKILLWSPVHQDDQFWLDNLDKFKENNWKLFLQLIDL 389
L E L+ +L+SFDEY E+ S L WSPVH+ +FW +N + E N++L L+ L
Sbjct: 268 LSEKLQNSVQDLSSFDEYATEVRSGRLEWSPVHKSAKFWRENAQRLNEKNYELLRILVHL 327
Query: 390 LK 391
L+
Sbjct: 328 LE 329
>At3g42050 [C] KOG2759 Vacuolar H+-ATPase V1 sector subunit H
Length = 441
Score = 71.2 bits (173), Expect = 3e-12
Identities = 57/224 (25%), Positives = 112/224 (49%), Gaps = 17/224 (7%)
Query: 232 YYALMIIWLLTF-DPKITEAFTKEHLAHYLQLLRLIRSTIKEKVVRLSVAIILNGINESV 290
Y + IWLL++ +P I T + +L +++ + KEKVVR+ + N + +
Sbjct: 218 YETCLCIWLLSYYEPAIEYLATSRTMQ---RLTEVVKHSTKEKVVRVVILTFRNLLPKGT 274
Query: 291 KNHKSTVKNLILLGNAIPTLNQIMERKFLDDELKQDLTTLKESLEAEYHELTSFDEYLAE 350
+ L + +++ T + + D++L L L+E L+ + +L+SFD+Y E
Sbjct: 275 FGAQMVDLGLPHIIHSLKT------QAWSDEDLLDALNQLEEGLKDKIKKLSSFDKYKQE 328
Query: 351 LNSKILLWSPVHQDDQFWLDNLDKFKENNWKLFLQLIDLLKEFITEKRPSSVSLQILLND 410
+ L W+P+H++ FW +N+ F+EN++++ L+ +L T P SL + D
Sbjct: 329 VLLGHLDWNPMHKETNFWRENVTCFEENDFQILRVLLTILD---TSSDPR--SLAVACFD 383
Query: 411 IRKVMELDNDSIKILG--KDKLIIMQLLQHSDSKVKYEALKTTQ 452
I + ++ I+ K K +M+L+ H +++V A+ Q
Sbjct: 384 ISQFIQYHAAGRVIVADLKAKERVMKLINHENAEVTKNAILCIQ 427
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,490,951
Number of Sequences: 60738
Number of extensions: 918693
Number of successful extensions: 3491
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3467
Number of HSP's gapped (non-prelim): 11
length of query: 460
length of database: 30,389,216
effective HSP length: 110
effective length of query: 350
effective length of database: 23,708,036
effective search space: 8297812600
effective search space used: 8297812600
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)