ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1625 good J KOG1936 Translation, ribosomal structure and biogenesis Histidyl-tRNA synthetase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1625 571704 573290 529
(529 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR033c [J] KOG1936 Histidyl-tRNA synthetase 814 0.0
SPBC2G2.12 [J] KOG1936 Histidyl-tRNA synthetase 572 e-163
Hs6996014 [J] KOG1936 Histidyl-tRNA synthetase 448 e-126
7293481 [J] KOG1936 Histidyl-tRNA synthetase 436 e-122
Hs15029520 [J] KOG1936 Histidyl-tRNA synthetase 415 e-115
At3g02760 [J] KOG1936 Histidyl-tRNA synthetase 391 e-108
CE06427 [J] KOG1936 Histidyl-tRNA synthetase 374 e-103
ECU06g0620 [J] KOG1936 Histidyl-tRNA synthetase 303 3e-82
At3g46100 [J] KOG1936 Histidyl-tRNA synthetase 143 7e-34
At3g59410 [J] KOG1035 eIF-2alpha kinase GCN2 116 7e-26
Hs20553830 [J] KOG1035 eIF-2alpha kinase GCN2 113 8e-25
YDR283c [J] KOG1035 eIF-2alpha kinase GCN2 99 2e-20
7302048 [J] KOG1035 eIF-2alpha kinase GCN2 83 1e-15
At5g03410_1 [J] KOG1936 Histidyl-tRNA synthetase 74 5e-13
>YPR033c [J] KOG1936 Histidyl-tRNA synthetase
Length = 546
Score = 814 bits (2102), Expect = 0.0
Identities = 403/502 (80%), Positives = 445/502 (88%), Gaps = 4/502 (0%)
Query: 28 GKLQFTLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGE 87
GKLQ +LKTPKGTKDWA+ DMVIREAIFS LS LFK+HGG+T+DTPVFELREILAGKYGE
Sbjct: 49 GKLQVSLKTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGE 108
Query: 88 DSKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMR 147
DSKLIYNLEDQGGELCSLRYDLTVPFARYVAMNN+QSIKRYHIAKVYRRDQPAMTKGRMR
Sbjct: 109 DSKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNIQSIKRYHIAKVYRRDQPAMTKGRMR 168
Query: 148 EFYQCDFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDE 207
EFYQCDFDVAGT+ESMVPD+E LSILVEGLTSLGIKDFK+K+NHRKILDGIFQIAGVKDE
Sbjct: 169 EFYQCDFDVAGTFESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVKDE 228
Query: 208 DVRKISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKA 267
DVRKISSAVDKLDKSPWE VKKE+T EKGQ+EETA+ IGEYVKLNGSL+ IH +LS D
Sbjct: 229 DVRKISSAVDKLDKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADAN 288
Query: 268 FQANEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKN 327
+NEKA +GL +I TL+ Y +AF I +ISFDLSLARGLDYYTG+IYE VT+ASAPP+N
Sbjct: 289 ITSNEKAKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPEN 348
Query: 328 AXXXXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGV 387
A +VGVGSIAAGGRYDNLVNMF EASGKKST IPCVGISFGV
Sbjct: 349 A----SELKKKAKSAEDASEFVGVGSIAAGGRYDNLVNMFSEASGKKSTQIPCVGISFGV 404
Query: 388 ERIFSLIKQRVEQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVY 447
ERIFSLIKQR+ S ++PTATQVFVMAFGGGKDWTGYLPERMKV K LW+ GIEAEYVY
Sbjct: 405 ERIFSLIKQRINSSTTIKPTATQVFVMAFGGGKDWTGYLPERMKVTKQLWDAGIEAEYVY 464
Query: 448 KSKANPRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRLGPEFANDDGELIDAADLISVV 507
K+KANPRKQFDAAEK+GC +AVILGKEEYLE KLRVKRLG EFA+DDGEL+ AAD++ +V
Sbjct: 465 KAKANPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRLGQEFADDDGELVSAADIVPIV 524
Query: 508 KEKLSQVHENGVDEVTRLIKGL 529
+EKLSQ+HE+G++EVTRLIKGL
Sbjct: 525 QEKLSQIHEDGLNEVTRLIKGL 546
>SPBC2G2.12 [J] KOG1936 Histidyl-tRNA synthetase
Length = 538
Score = 572 bits (1475), Expect = e-163
Identities = 284/481 (59%), Positives = 362/481 (75%), Gaps = 7/481 (1%)
Query: 32 FTLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSKL 91
FTLKTPKGTKDW +KD+V+RE IFS ++ +FKRHGG+TLDTPVFEL+EIL+GKYGEDSKL
Sbjct: 61 FTLKTPKGTKDWCDKDVVLREHIFSTVTEIFKRHGGVTLDTPVFELKEILSGKYGEDSKL 120
Query: 92 IYNLEDQGGELCSLRYDLTVPFARYVAMN-NVQSIKRYHIAKVYRRDQPAMTKGRMREFY 150
IY+L+DQGGELCSLRYDLTVPFAR++AMN V SIKR+HIAKVYRRDQPAMTKGRMREFY
Sbjct: 121 IYDLKDQGGELCSLRYDLTVPFARWLAMNPKVTSIKRFHIAKVYRRDQPAMTKGRMREFY 180
Query: 151 QCDFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDEDVR 210
QCDFD+AG Y+ M+PD+E L +LVE L +L + ++ +K+NHRKILDGIF + GV D+ +R
Sbjct: 181 QCDFDIAGNYDPMIPDSEALKVLVEALDALEVGNYTIKLNHRKILDGIFTVCGVPDDKIR 240
Query: 211 KISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQA 270
ISSAVDKLDK PWE V++E+ +EKG EE A+ I EYV L G ++ D L D +
Sbjct: 241 TISSAVDKLDKLPWEDVRREMVVEKGLKEEVADKIKEYVLLKGD-RSLLDKLEADSLLSS 299
Query: 271 NEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXX 330
N AV ++ L Y++AFG+ D SFD+SLARGLDYYTGIIYEAVT ASA PK
Sbjct: 300 NSSAVAAFNDMRLLFDYLEAFGVLDRFSFDMSLARGLDYYTGIIYEAVTEASA-PKIKSS 358
Query: 331 XXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVERI 390
+GVGSIAAGGRYDNLV MF + KK+ IPCVGIS G+ERI
Sbjct: 359 AEKKKSADPEADRSNDDSIGVGSIAAGGRYDNLVGMF---AAKKNAKIPCVGISLGLERI 415
Query: 391 FSLIKQRVEQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVYKSK 450
FS+++ ++ ++R VFVMAFGGGK+WTG+L ERM V K LW NGI+AE++YK K
Sbjct: 416 FSILRSKI-PDEDIRANDVDVFVMAFGGGKEWTGFLKERMSVCKDLWANGIKAEFLYKVK 474
Query: 451 ANPRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRLGPEFANDDGELIDAADLISVVKEK 510
PR+QFDAA+K P AVILG++E+ + +R+K++G + +D+G+L+ +++SV+K+
Sbjct: 475 PKPRQQFDAADKFDVPFAVILGQDEFAKGSVRIKQMGLKENSDEGQLVPREEMVSVLKKL 534
Query: 511 L 511
L
Sbjct: 535 L 535
>Hs6996014 [J] KOG1936 Histidyl-tRNA synthetase
Length = 509
Score = 448 bits (1153), Expect = e-126
Identities = 243/486 (50%), Positives = 317/486 (65%), Gaps = 33/486 (6%)
Query: 29 KLQFTLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGED 88
K +F LKTPKGT+D++ + M +RE +F + FKRHG +DTPVFEL+E L GKYGED
Sbjct: 51 KQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGED 110
Query: 89 SKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMRE 148
SKLIY+L+DQGGEL SLRYDLTVPFARY+AMN + +IKRYHIAKVYRRD PAMT+GR RE
Sbjct: 111 SKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYRE 170
Query: 149 FYQCDFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDED 208
FYQCDFD+AG ++ M+PDAE L I+ E L+SL I DF VK+N R+ILDG+F I GV D
Sbjct: 171 FYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSK 230
Query: 209 VRKISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAF 268
R I S+VDKLDK WE VK E+ EKG E A+ IG+YV+ +G + + +L K
Sbjct: 231 FRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLS 290
Query: 269 QANEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNA 328
Q N++A+EGL +++ L Y+ FGI D ISFDLSLARGLDYYTG+IYEAV + P A
Sbjct: 291 Q-NKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQT--PAQA 347
Query: 329 XXXXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVE 388
+GVGS+AAGGRYD LV MF + G+K +PCVG+S GVE
Sbjct: 348 GEEP----------------LGVGSVAAGGRYDGLVGMF-DPKGRK---VPCVGLSIGVE 387
Query: 389 RIFSLIKQRVEQ-SNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVY 447
RIFS+++QR+E +R T TQV V L ER+K+ LW+ GI+AE +Y
Sbjct: 388 RIFSIVEQRLEALEEKIRTTETQVLV-----ASAQKKLLEERLKLVSELWDAGIKAELLY 442
Query: 448 KSKANPRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRLGPEFANDDGELIDAADLISVV 507
K Q E++G PL I+G++E + ++++ + D + DL+ +
Sbjct: 443 KKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVD----VRREDLVEEI 498
Query: 508 KEKLSQ 513
K + Q
Sbjct: 499 KRRTGQ 504
>7293481 [J] KOG1936 Histidyl-tRNA synthetase
Length = 522
Score = 436 bits (1121), Expect = e-122
Identities = 227/462 (49%), Positives = 307/462 (66%), Gaps = 26/462 (5%)
Query: 31 QFTLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSK 90
+FTLKTPKGT+D+ + M +R+ + ++ +FKRHGG +DTPVFEL+E+L GKYGEDSK
Sbjct: 56 KFTLKTPKGTRDYGPQQMTLRQGVLDKIVQVFKRHGGEAIDTPVFELKEVLTGKYGEDSK 115
Query: 91 LIYNLEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFY 150
LIY+L+DQGGE+ S+RYDLTVP ARY+AMN + SIKRYHIAKVYRRD PAMTKGR REFY
Sbjct: 116 LIYDLKDQGGEILSMRYDLTVPLARYLAMNKISSIKRYHIAKVYRRDNPAMTKGRYREFY 175
Query: 151 QCDFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDEDVR 210
QCDFD+AGTY+ M+PDAE + I+ E L +L I D+ +K+NHR++LDG+FQ GV + R
Sbjct: 176 QCDFDIAGTYDPMLPDAECVKIVSEILDTLDIGDYVIKLNHRQLLDGMFQACGVPADSFR 235
Query: 211 KISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQA 270
I SAVDKLDKSPW V+KE+ EKG E A+ IGEYV+L+G E + +L+ +K +A
Sbjct: 236 TICSAVDKLDKSPWADVRKEMVDEKGLDEAAADRIGEYVRLSGGAELVEQLLANEK-LKA 294
Query: 271 NEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAV-----TAASAPP 325
AV+GL+ ++ L+ Y FG+ +SFDLSLARGLDYYTG+IYE V ++P
Sbjct: 295 VPNAVKGLEGMKQLLKYCSIFGLDKRVSFDLSLARGLDYYTGVIYEGVLKGESATVASPA 354
Query: 326 KNAXXXXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISF 385
K + VGS+A GGRYDNLV MF + GK ++PCVG+S
Sbjct: 355 KTSQQNGEQANEPAT----------VGSVAGGGRYDNLVGMF-DPRGK---AVPCVGVSI 400
Query: 386 GVERIFSLIKQRVEQSN-NVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAE 444
GVERIFS+++ R S +R + +V+V G +R+KV +LW+ G++AE
Sbjct: 401 GVERIFSVLEARAAASGLKLRTSDVEVYV-----ASAHKGLHEQRLKVLNLLWDAGVKAE 455
Query: 445 YVYKSKANPRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRL 486
+ YK Q E+ PL V+LG E + ++++ +
Sbjct: 456 HSYKLNPKLLVQLQHCEEHQIPLVVVLGDAELAQGLVKLREV 497
>Hs15029520 [J] KOG1936 Histidyl-tRNA synthetase
Length = 506
Score = 415 bits (1066), Expect = e-115
Identities = 221/491 (45%), Positives = 313/491 (63%), Gaps = 43/491 (8%)
Query: 29 KLQFTLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGED 88
K F +KTPKGT+D + + MV+RE I + S FKRHG +DTP FEL+E L KYGED
Sbjct: 52 KPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGED 111
Query: 89 SKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMRE 148
S L+Y+L+DQGGEL SLRYDLTVPFARY+AMN V+ +KRYH+ KV+RR+ P + +GR RE
Sbjct: 112 SGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYRE 171
Query: 149 FYQCDFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDED 208
F QCDFD+AG ++ M+PDAE L I+ E L+ L + DF +K+N R+I+DG+F + GV +
Sbjct: 172 FCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESK 231
Query: 209 VRKISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAF 268
R I S++DKLDK W+ V+ E+ ++KG E A+ IG+YV+ +G + + + +D
Sbjct: 232 FRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMF-QDPRL 290
Query: 269 QANEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAV-----TAASA 323
N++A+EGL +++ L Y+ FGI D ISFDLSLARGLDYYTG+IYEAV T A
Sbjct: 291 SQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGE 350
Query: 324 PPKNAXXXXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGI 383
P N VGS+AAGGRYD LV MF K +PCVG+
Sbjct: 351 EPLN-----------------------VGSVAAGGRYDGLVGMF----DPKGHKVPCVGL 383
Query: 384 SFGVERIFSLIKQRVE-QSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIE 442
S GVERIF +++QR++ + VR T TQVFV +L ER+K+ LW++GI+
Sbjct: 384 SIGVERIFYIVEQRMKTKGEKVRTTETQVFV-----ATPQKNFLQERLKLIAELWDSGIK 438
Query: 443 AEYVYKSKANPRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRLGPEFANDDGELIDAAD 502
AE +YK+ Q E +G PL VI+G++E E ++++ + A+ + I +
Sbjct: 439 AEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSV----ASREEVAIKREN 494
Query: 503 LISVVKEKLSQ 513
++ ++++LS+
Sbjct: 495 FVAEIQKRLSE 505
>At3g02760 [J] KOG1936 Histidyl-tRNA synthetase
Length = 479
Score = 391 bits (1004), Expect = e-108
Identities = 217/454 (47%), Positives = 286/454 (62%), Gaps = 44/454 (9%)
Query: 35 KTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSKLIYN 94
K PKGT+D+A++ M +RE FS + ++FKRHG LDTPVFELRE L GKYGEDSKL+Y+
Sbjct: 43 KLPKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMGKYGEDSKLVYD 102
Query: 95 LEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFYQCDF 154
+ DQGGELCSLRYDLTVPFARYVAMN + S KRY IAKVYRRD P +KGR REFYQCDF
Sbjct: 103 IADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRYREFYQCDF 160
Query: 155 DVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDEDVRKISS 214
D+AG +E M PD EI+ IL E L L I D++VK+NHRK+LDG+ +I GV E R I S
Sbjct: 161 DIAGLFEPMGPDFEIVKILTELLDELEIGDYEVKLNHRKLLDGMLEICGVPPEKFRTICS 220
Query: 215 AVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGS-LETIHDILSKDKAFQANEK 273
++DKLDK +E VKKE+ EKG + E A+ IG +VK G+ LE + + + F N+
Sbjct: 221 SIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQEGSEFLDNQS 280
Query: 274 AVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXXXXX 333
+ E L E+ + +K + I FDLSLARGLDYYTG+I+EAV +
Sbjct: 281 SREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAE---------- 330
Query: 334 XXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVERIFSL 393
VGSI AGGRYDNL+ MFG + +P VG+S G+ER+F++
Sbjct: 331 -----------------VGSIGAGGRYDNLIGMFG------TKQVPAVGMSLGIERVFNI 367
Query: 394 IKQRVE-QSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVYKSKAN 452
+++ E Q +RPT TQV V K L E ++ LW I AEY+ +
Sbjct: 368 MEELNEKQKQVIRPTETQVLVSIMVDNK-----LAEAAELVSQLWGAKINAEYLVSKR-- 420
Query: 453 PRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRL 486
K F+ A++SG P V++G++E + + +K+L
Sbjct: 421 KEKHFNRAKESGIPWMVMVGEKELSGSFVTLKKL 454
>CE06427 [J] KOG1936 Histidyl-tRNA synthetase
Length = 520
Score = 374 bits (959), Expect = e-103
Identities = 211/495 (42%), Positives = 304/495 (60%), Gaps = 35/495 (7%)
Query: 24 PKSGGKLQFTLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAG 83
P+ GK F LKT KGT+D+ +R ++ ++ F R+G T+DTPVFELR++L G
Sbjct: 53 PEKPGK--FVLKTGKGTRDYGPAQSALRNSVLQTVTETFNRYGAETIDTPVFELRDVLMG 110
Query: 84 KYGEDS-KLIYNLEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMT 142
KYGE+ KL+Y+L+DQGGEL SLRYDLTVPFARY+AMN + +I RY IAKVYRRDQP M+
Sbjct: 111 KYGEEGGKLVYDLQDQGGELLSLRYDLTVPFARYLAMNKITNITRYQIAKVYRRDQPVMS 170
Query: 143 KGRMREFYQCDFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIA 202
+GR REFYQCDFD+AG Y+ M+P+AE L I+ E LT L I +F + +NHR IL+G+F ++
Sbjct: 171 RGRYREFYQCDFDIAGQYDLMLPEAECLGIVDELLTKLEIGEFFINLNHRLILEGMFAVS 230
Query: 203 GVKDEDVRKISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVK---LNGSLETIH 259
G+ +D + I S+VDKLDK+PWE V++E+ EK T+E +GE V+ LN L +
Sbjct: 231 GIPAKDFKTICSSVDKLDKTPWEDVEQEMINEKFLTKEQTGKLGELVRFRELNSDLNNLE 290
Query: 260 --DILSKDKAFQANEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEA 317
+ +S+ N+K +G +E++ LI Y+ G+ + ++ SLARGLDYYTG IYEA
Sbjct: 291 LLEKMSQLPDLGQNDKFKKGAEELKVLIEYLNVDGVTT-VRYEPSLARGLDYYTGAIYEA 349
Query: 318 VTAASAPPKNAXXXXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTS 377
V PK VGVGS+AAGGRYD LV MF +
Sbjct: 350 VA-----PK-----ALEGTAVENSEDTAGQPVGVGSVAAGGRYDGLVKMF-----DSKAN 394
Query: 378 IPCVGISFGVERIFSLIKQRVEQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLW 437
+PC G+SFG+ER+F++++ R Q +R T T+V+V + +R K+ KML
Sbjct: 395 VPCCGVSFGIERLFAIMEAR--QKVAIRTTQTEVYV-----ASAQKNLVRDRKKLVKMLR 447
Query: 438 ENGIEAEYVYKSKANPRKQFDAAEKSGCPLAVILGKEEYLENKLRVKRLGPEFANDDGEL 497
GI+ E K+ QF AE+ PLA+++G++E + ++++ + D +
Sbjct: 448 SAGIKTEMALKANPKLLTQFQYAEERRIPLAIVIGEQELKDGVVKLRNV----VTRDEQT 503
Query: 498 IDAADLISVVKEKLS 512
I LI+ V++ L+
Sbjct: 504 IKLDQLITAVRDTLA 518
>ECU06g0620 [J] KOG1936 Histidyl-tRNA synthetase
Length = 435
Score = 303 bits (777), Expect = 3e-82
Identities = 171/453 (37%), Positives = 257/453 (55%), Gaps = 48/453 (10%)
Query: 33 TLKTPKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSKLI 92
+LKTPKGT D + + + E I ++ +F +G + + TP FE++ IL KYGED+KLI
Sbjct: 3 SLKTPKGTMDESPRQCFLHEEIIERIRRVFVLYGAVPISTPTFEMKSILTNKYGEDTKLI 62
Query: 93 YNLEDQGGELCSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFYQC 152
Y+L+DQGGE+C+LRYDLTVPFARY+A N ++ +KRY I +VYRRDQP++ +GR+REF Q
Sbjct: 63 YDLKDQGGEICALRYDLTVPFARYLASNKIRRMKRYQIGRVYRRDQPSIARGRLREFVQA 122
Query: 153 DFDVAGTYESMVPDAEILSILVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDEDVRKI 212
DFD+AG M+ DAE++S + L+ GI ++++K+N R+IL I ++ GV E +
Sbjct: 123 DFDIAGECIQMMADAEVVSCVDRILSMFGIGEYRIKVNDRRILTSILEVVGVSCESYGTV 182
Query: 213 SSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQANE 272
S +DK++K WE + +E+ + KG +EE +Y+ + G E + +L D + E
Sbjct: 183 CSTIDKIEKMSWEDIGRELVL-KGLSEEQVRTTKKYMTMGGK-EDVLGLLKADPVY-GIE 239
Query: 273 KAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXXXX 332
+ + ++E L + G + D+SLARGLDYYTG+I EA
Sbjct: 240 RCKAAVHDMEELFRLCRILGCSGSLVMDISLARGLDYYTGMILEA--------------- 284
Query: 333 XXXXXXXXXXXXXXXYVG--VGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVERI 390
YVG VGS+ GGRYDNL GE + S PCVG S GV RI
Sbjct: 285 --------------EYVGKSVGSVIGGGRYDNLTENLGE----RCVSTPCVGFSVGVSRI 326
Query: 391 FSLIKQRVEQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVYKSK 450
FSL+ + + + ++T V+V A GG +L ER+ V ++L + I +E Y +
Sbjct: 327 FSLLYEEYD-----KESSTMVYVGASGG-----LFLDERLSVLRILQDANICSETFYTRR 376
Query: 451 ANPRKQFDAAEKSGCPLAVILGKEEYLENKLRV 483
++ Q K P V++G+ E ++V
Sbjct: 377 SSFESQQKYVAKKKIPFIVVVGESEIAAGSVQV 409
>At3g46100 [J] KOG1936 Histidyl-tRNA synthetase
Length = 486
Score = 143 bits (360), Expect = 7e-34
Identities = 123/457 (26%), Positives = 211/457 (45%), Gaps = 63/457 (13%)
Query: 37 PKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSK-LIYNL 95
PKGT+D+ +DM +R +F+ + + +G +D PV E + K GE+ + +Y
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCF 131
Query: 96 EDQGGELCSLRYDLTVPFARYVAMNNVQ---SIKRYHIAKVYRRDQPAMTKGRMREFYQC 152
ED+G +LR +LT AR V +K + I + +R ++ MT+GR RE YQ
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYER--MTRGRRREHYQW 189
Query: 153 DFDVAGTYESMVPDAEILSILVEGLTSLGI--KDFKVKMNHRKILDGIFQIAGVKDEDVR 210
+ D+ G + + +AE++S +V +GI D K++ RK+L + + GV ++
Sbjct: 190 NMDIIGVPQ-VTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFG 248
Query: 211 KISSAVDKLDKSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQA 270
++ +DK++K P + +KKE+ G +E+ + + + + SL+ + DI+
Sbjct: 249 RVCIIIDKIEKIPIDEIKKELGFT-GISEDAIEQLLQVLSVK-SLDDLEDIIGG------ 300
Query: 271 NEKAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXX 330
A E + +++ L S + FG ++I FD S+ RGL YYTGI++E
Sbjct: 301 ---AGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKGK------- 350
Query: 331 XXXXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVERI 390
+ +I GGRYD L++ +G P G FG I
Sbjct: 351 --------------------LRAICGGGRYDRLLSTYG------GDDFPACGFGFGDAVI 384
Query: 391 FSLIKQRVEQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVYKSK 450
L+K++ + + +V + KD G VA L + G + V +SK
Sbjct: 385 VELLKEK----DLLPELGQEVENIVCALDKDLQG---AAATVATALRDKGQTVDLVLESK 437
Query: 451 ANPRK-QFDAAEKSGCPLAVILGKEEYLENKLRVKRL 486
P K F A + V++GK E+ + + VK L
Sbjct: 438 --PLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVL 472
>At3g59410 [J] KOG1035 eIF-2alpha kinase GCN2
Length = 1271
Score = 116 bits (291), Expect = 7e-26
Identities = 106/449 (23%), Positives = 200/449 (43%), Gaps = 46/449 (10%)
Query: 43 WAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSKLIYNLEDQGGEL 102
+ E + +R+ + +F++H L+ V +R L + S+ L GG++
Sbjct: 819 YTEINTELRDYVVEITKEVFRQHCAKHLE--VIPMR--LLSDCPQFSRKTVKLLTNGGDM 874
Query: 103 CSLRYDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDVAGTYES 162
L Y+L +PF ++++N S KRY I+ VYRR A+ Q DFD+ G S
Sbjct: 875 LELCYELRLPFVHWISVNQKSSFKRYEISHVYRR---AIGHSPPNPCLQADFDIVGGTLS 931
Query: 163 MVPDAEILSILVEGLTSLGIK-DFKVKMNHRKILDGIFQIAGVKDEDVRKISSAVDKLD- 220
+ +AE+L ++V+ T + + + +NH +LD I+ AG+K E RK++ + +
Sbjct: 932 LT-EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGS 990
Query: 221 --------KSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQANE 272
K W +++++ E E N + + V + + A +A+
Sbjct: 991 LRPQSSERKLKWVFIRRQLLQELKLPEAVVNRL-QTVASRFCGDADQALPRLRGALRADR 1049
Query: 273 KAVEGLKEIETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXXXX 332
+ L E+ L++Y++ + I++++ D+ + Y+ + ++ +N+
Sbjct: 1050 PTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLT----KENSSGTS 1105
Query: 333 XXXXXXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVERIFS 392
GV +A GGRYD LV + K + VG+S +E IF
Sbjct: 1106 ND---------------GV-LLAVGGRYDWLVQEVCDREHKMNLP-GAVGVSLALETIFQ 1148
Query: 393 LIKQRVEQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVYKSKAN 452
+ + N +T V V + GGG G L +RM++ LWE I+AE+V +
Sbjct: 1149 HLPMDLRPIRN--EVSTSVLVCSRGGG----GLLVQRMELVAELWEKSIKAEFVPTPDPS 1202
Query: 453 PRKQFDAAEKSGCPLAVILGKEEYLENKL 481
+Q++ A + VI+ + +N++
Sbjct: 1203 LTEQYEYANEHEIKCLVIITESGVAQNQI 1231
>Hs20553830 [J] KOG1035 eIF-2alpha kinase GCN2
Length = 1528
Score = 113 bits (282), Expect = 8e-25
Identities = 95/409 (23%), Positives = 184/409 (44%), Gaps = 52/409 (12%)
Query: 50 IREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDL 109
+++ + + +FKRHG + L TP+ R ++ E + + D G L L +DL
Sbjct: 942 MQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFM----DHSGMLVMLPFDL 997
Query: 110 TVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFD-VAGTYESMVPDAE 168
+PFARYVA NN+ ++KRY I +V+R + + + +E +C FD V T S +P AE
Sbjct: 998 RIPFARYVARNNILNLKRYCIERVFRPRK--LDRFHPKELLECAFDIVTSTTNSFLPTAE 1055
Query: 169 ILSI---LVEGLTSLGIKDFKVKMNHRKILDGIFQIAGVKDEDVRKI-----SSAVDKLD 220
I+ +++ +L +++ + +NH +L I G+ ++ + ++ + +KL
Sbjct: 1056 IIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLT 1115
Query: 221 KSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQANEKAVEGLKE 280
+ E +++ I + L + TI+ ++ + K GLK+
Sbjct: 1116 RREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVK--YGLKD 1173
Query: 281 IETLISYVKAFGIQDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXXXXXXXXXXXX 340
+E ++ +K GI+ + +L L + + GII++ V +
Sbjct: 1174 LEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEI--------- 1224
Query: 341 XXXXXXXYVGVGSIAAGGRYDNLVNMF--GEASGKKSTSIPCVGISFGVERIFSLIKQRV 398
+AAGGRYD L+ F +A G T+I G+S +++I + + +
Sbjct: 1225 -------------LAAGGRYDLLIPQFRGPQALGPVPTAI---GVSIAIDKISAAV---L 1265
Query: 399 EQSNNVRPTATQVFVMAFGGGKDWTGYLPERMKVAKMLWENGIEAEYVY 447
+V ++ + V++ G + + + + LW GI AE +Y
Sbjct: 1266 NMEESVTISSCDLLVVSVGQMS-----MSRAINLTQKLWTAGITAEIMY 1309
>YDR283c [J] KOG1035 eIF-2alpha kinase GCN2
Length = 1659
Score = 98.6 bits (244), Expect = 2e-20
Identities = 105/425 (24%), Positives = 179/425 (41%), Gaps = 68/425 (16%)
Query: 49 VIREAIFSQLSSLFKRHGGITLDTP--VFELREILAGKYGEDSKLIYNLEDQGGELCSLR 106
++R + ++ +F++HGGI + P +F I YG + +Y + D+GG + L+
Sbjct: 1038 ILRSQMTEEVVKIFRKHGGIENNAPPRIFPKAPI----YGTQN--VYEVLDKGGTVLQLQ 1091
Query: 107 YDLTVPFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDV--AGTYESMV 164
YDLT P ARY++ N K+Y + VYR + + R+F + DFD+ + ES
Sbjct: 1092 YDLTYPMARYLSKNPSLISKQYRMQHVYRPPDHSRSSLEPRKFGEIDFDIISKSSSESGF 1151
Query: 165 PDAEILSILVEGLTSLGI----KDFKVKMNHRKILDGIFQIAGVKDEDVRKISSAVDKLD 220
DAE L I+ E LT + F + +NH IL+ +F + +S + ++
Sbjct: 1152 YDAESLKIIDEILTVFPVFEKTNTFFI-LNHADILESVFNFTNIDKAQRPLVSRMLSQVG 1210
Query: 221 -KSPWEVVKKEITIEKGQTEETANIIGEYVKLNGSLETIHDILSKDKAFQANEKAVE-GL 278
++ VK E+ + + N + E E L K + K +E L
Sbjct: 1211 FARSFKEVKNELKAQLNISSTALNDL-ELFDFRLDFEAAKKRLYKLMIDSPHLKKIEDSL 1269
Query: 279 KEIETLISYVKAFGI-QDYISFDLSLARGLDYYTGIIYEAVTAASAPPKNAXXXXXXXXX 337
I ++SY+K + ++ + LS Y GI++ AV +
Sbjct: 1270 SHISKVLSYLKPLEVARNVVISPLSNYNSAFYKGGIMFHAVYDDGS-------------- 1315
Query: 338 XXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSI-PCVGISFGVERIFSLIK- 395
IAAGGRYD L++ F SGKKS++ VG + E IF + +
Sbjct: 1316 ------------SRNMIAAGGRYDTLISFFARPSGKKSSNTRKAVGFNLAWETIFGIAQN 1363
Query: 396 -------QRVEQSNNV--------RPTATQVFVMAFGGG-KDWTGYLPERMKVAKMLWEN 439
R+++ N +P+ V + +F D G + + LW+
Sbjct: 1364 YFKLASGNRIKKRNRFLKDTAVDWKPSRCDVLISSFSNSLLDTIG-----VTILNTLWKQ 1418
Query: 440 GIEAE 444
I+A+
Sbjct: 1419 NIKAD 1423
>7302048 [J] KOG1035 eIF-2alpha kinase GCN2
Length = 1589
Score = 82.8 bits (203), Expect = 1e-15
Identities = 88/359 (24%), Positives = 157/359 (43%), Gaps = 40/359 (11%)
Query: 52 EAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTV 111
E + +++ +LF++HG I +D+P+ ++ ++ L G + L DL
Sbjct: 972 EFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVH-LMTHSGCVVVLPCDLRT 1030
Query: 112 PFARYVAMNNVQSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFD-VAGTYESMVPDAEIL 170
FAR+V MN+V I+RY + +VYR ++ + ++ Y C FD +A T S + DAE+L
Sbjct: 1031 QFARHVTMNSVNLIRRYCVDRVYREER--VFNFHPKQSYDCSFDIIAPTTGSHLVDAELL 1088
Query: 171 SILVE---GLTSLGIKDFKVKMNHRKILDGIFQIAGVKDEDVRKISSAVDKLDKSPWEVV 227
S+ E L L K+ ++MNH +L I V + +S
Sbjct: 1089 SLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRF 1148
Query: 228 KKEITI----EKGQT--EETANIIGEYVKLNGSLETIHDILSKD---KAFQANEKAVEGL 278
+ +I EK +T + +++ L GS T+ D K +A A L
Sbjct: 1149 QFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGAL 1208
Query: 279 KEIETLISYVKAFGIQDYISFDLSLARGLDYYT--GIIYEAVTAASAPPKNAXXXXXXXX 336
+E+ET++ + G++ I L D + GI+++ +TA P ++
Sbjct: 1209 RELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQ-MTADLKPNRSGHPSV---- 1263
Query: 337 XXXXXXXXXXXYVGVGSIAAGGRYDNLVNMFGEASGKKSTSIPCVGISFGVERIFSLIK 395
+A G RYD++++ F + + K + ++P G+ G FSL K
Sbjct: 1264 -----------------LAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSLDK 1305
>At5g03410_1 [J] KOG1936 Histidyl-tRNA synthetase
Length = 395
Score = 73.9 bits (180), Expect = 5e-13
Identities = 56/190 (29%), Positives = 96/190 (50%), Gaps = 11/190 (5%)
Query: 37 PKGTKDWAEKDMVIREAIFSQLSSLFKRHGGITLDTPVFELREILAGKYGEDSK-LIYNL 95
PKGT+D+ +DM +R +F+ + + G +D PV E + K GE+ + +Y
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCF 131
Query: 96 EDQGGELCSLRYDLTVPFARYVAMNNVQ---SIKRYHIAKVYRRDQPAMTKGRMREFYQC 152
ED+G +LR +LT AR V +K + + + +R ++ MT+GR RE YQ
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYER--MTRGRRREHYQW 189
Query: 153 DFDVAGTYESMVPDAEILSILVEGLTSLGI--KDFKVKMNHRKILDGIFQIAGVKD--ED 208
+ D+ G E + +AE++S ++ +GI D K++ RK A + + ++
Sbjct: 190 NMDIIGVPE-VTAEAELISSIMTFFKRIGITASDVGFKVSSRKQSKHQLTAALLPELGQE 248
Query: 209 VRKISSAVDK 218
V I A+DK
Sbjct: 249 VENIVCALDK 258
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,856,160
Number of Sequences: 60738
Number of extensions: 1188289
Number of successful extensions: 3370
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3312
Number of HSP's gapped (non-prelim): 21
length of query: 529
length of database: 30,389,216
effective HSP length: 111
effective length of query: 418
effective length of database: 23,647,298
effective search space: 9884570564
effective search space used: 9884570564
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)