ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1674 good U KOG2887 Intracellular trafficking, secretion, and vesicular transport Membrane protein involved in ER to Golgi transport
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1674 589706 590344 213
(213 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBL102w [U] KOG2887 Membrane protein involved in ER to Golgi tra... 338 3e-93
SPAC1527.02 [U] KOG2887 Membrane protein involved in ER to Golgi... 125 3e-29
CE20520 [U] KOG2887 Membrane protein involved in ER to Golgi tra... 101 9e-22
At5g56020 [U] KOG2887 Membrane protein involved in ER to Golgi t... 85 8e-17
At4g26550 [U] KOG2887 Membrane protein involved in ER to Golgi t... 74 1e-13
At5g23550 [U] KOG2887 Membrane protein involved in ER to Golgi t... 60 3e-09
Hs14736892 [U] KOG2887 Membrane protein involved in ER to Golgi ... 50 2e-06
7296331 [U] KOG2887 Membrane protein involved in ER to Golgi tra... 49 4e-06
ECU09g0940 [U] KOG2887 Membrane protein involved in ER to Golgi ... 49 5e-06
>YBL102w [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 215
Score = 338 bits (867), Expect = 3e-93
Identities = 156/215 (72%), Positives = 190/215 (87%), Gaps = 2/215 (0%)
Query: 1 MSENNP--EVNNLRDSLNRWNESRNAHSQGFNEGAKTIFASWADSINERAQDVYQRLPLT 58
MSE P +VN+LRDSLNRWN++R +SQGFNE AKT+F+SWADS+N RAQD+YQ LP++
Sbjct: 1 MSEEPPSDQVNSLRDSLNRWNQTRQQNSQGFNESAKTLFSSWADSLNTRAQDIYQTLPVS 60
Query: 59 QQDLTANPEPEWFSLSRTERLSLFVCFILGAVACFTICVFLFPVLAVQPRKFGLLWSMGS 118
+QDL + EP WF LSRTER+ LFVCF+LGA ACFT+C FLFPVLA +PRKFGLLW+MGS
Sbjct: 61 RQDLVQDQEPSWFQLSRTERMVLFVCFLLGATACFTLCTFLFPVLAAKPRKFGLLWTMGS 120
Query: 119 LLFIFAFGVLQGPVAYMRHLTSRERLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILEL 178
LLF+ AFGVL GP+AY++HLT+RERLPF++FFF +CF+TIYFAAF+K+T+LTI CA+LEL
Sbjct: 121 LLFVLAFGVLMGPLAYLKHLTARERLPFSMFFFATCFMTIYFAAFSKNTVLTITCALLEL 180
Query: 179 IAVIYYAISYFPFGGTGLRMISSIGLNQARGVLRI 213
+AVIYYAISYFPFG TGLRM+SS G+N ARGVLRI
Sbjct: 181 VAVIYYAISYFPFGATGLRMLSSAGVNSARGVLRI 215
>SPAC1527.02 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 201
Score = 125 bits (315), Expect = 3e-29
Identities = 66/153 (43%), Positives = 94/153 (61%), Gaps = 4/153 (2%)
Query: 52 YQRLPLTQQDLTANPEPEWFSLSRTERLSLFVCFILGAVACFTICVFLFPVLAVQPRKFG 111
Y +P + + FSLSR ER LF +LG++AC+ I F+FPVL ++PRKF
Sbjct: 41 YTEIPTNASGGNSYFQSSEFSLSRWERYMLFGICLLGSLACYAIACFMFPVLVLKPRKFV 100
Query: 112 LLWSMGSLLFIFAFGVLQGPVAYMRHLTSRERLPFTVFFFVSCFLTIYFAAFTKSTLLTI 171
LLW+MGSLL + F ++QG VA+ R LT+ ERLP T+ +FV+ TI KST+L+I
Sbjct: 101 LLWTMGSLLAVLGFAIVQGFVAHFRQLTTMERLPITLSYFVTLLATIIATIKIKSTILSI 160
Query: 172 PCAILELIAVIYYAISYFPFG----GTGLRMIS 200
+L +++ + Y I++FPFG G RM S
Sbjct: 161 VFGVLHILSFVAYLIAFFPFGTRTVSLGTRMAS 193
>CE20520 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 235
Score = 101 bits (251), Expect = 9e-22
Identities = 49/132 (37%), Positives = 80/132 (60%)
Query: 71 FSLSRTERLSLFVCFILGAVACFTICVFLFPVLAVQPRKFGLLWSMGSLLFIFAFGVLQG 130
F ++RT+R+ F I+GA+ CF+ L PV+ V RKF L ++GSLL + +F L G
Sbjct: 88 FGMTRTQRIIAFFMCIIGAIFCFSTAAVLIPVILVSTRKFAGLNTLGSLLLLLSFAFLLG 147
Query: 131 PVAYMRHLTSRERLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILELIAVIYYAISYFP 190
P +Y+ H+ S +R TV + + F T+Y + + KST+ T+ AI + +++Y +SY P
Sbjct: 148 PKSYLTHMASPQRRLVTVSYLSALFATLYSSLWLKSTIFTLIAAIFQGFTLVWYVLSYVP 207
Query: 191 FGGTGLRMISSI 202
G GL ++S+
Sbjct: 208 GGERGLFFMTSL 219
>At5g56020 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 230
Score = 84.7 bits (208), Expect = 8e-17
Identities = 53/185 (28%), Positives = 91/185 (48%), Gaps = 3/185 (1%)
Query: 18 WNESRNAHSQGFNEGAKTIFASWADSINERAQDVYQRLPLTQQDLTANPEPEWFSLSRTE 77
WN A S+ F E T+ ++ V + + + N + S+ +
Sbjct: 36 WNSY--AASRDFEEDTGTLGFDIESAVRSANDTVSGTFNVVSKGVRDNLQSATSSMPSGK 93
Query: 78 RLSLFVCFILGAVACFTICVFLF-PVLAVQPRKFGLLWSMGSLLFIFAFGVLQGPVAYMR 136
L F + V I +F PV+ + P+KF + +++G I +F L+GP +
Sbjct: 94 ALMYFGLLLASGVFFIFIAFTMFLPVMVLMPQKFAICFTLGCGFIIGSFFALRGPQNQLA 153
Query: 137 HLTSRERLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILELIAVIYYAISYFPFGGTGL 196
H++S ERLP T+ F + TIY + S +L++ + L++IA+ YY ISYFP G +G+
Sbjct: 154 HMSSAERLPLTLGFIATMVGTIYVSMVLHSYILSVIFSALQVIALAYYCISYFPGGSSGM 213
Query: 197 RMISS 201
+ +SS
Sbjct: 214 KFLSS 218
>At4g26550 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 385
Score = 74.3 bits (181), Expect = 1e-13
Identities = 40/130 (30%), Positives = 68/130 (51%), Gaps = 16/130 (12%)
Query: 88 GAVACFTICVFLFPVLAVQPRKFGLLWSMGSLLFIFAFGVLQGPVAYMRHLTSRE----- 142
G F PV+ + P+KF + +++G I +F L+GP + H++S E
Sbjct: 244 GVFFIFIAFTMFLPVMVLMPQKFAICFTLGCGFIIGSFFALRGPKNQLAHMSSMEVCYTV 303
Query: 143 -----------RLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILELIAVIYYAISYFPF 191
RLP T+ F + TIY + S +L++ ++L+++A++YY ISYFP
Sbjct: 304 LLCVCHALNPRRLPSTLGFIATMVGTIYVSMVLHSYILSVLFSVLQVLALVYYCISYFPG 363
Query: 192 GGTGLRMISS 201
G +G+R +SS
Sbjct: 364 GSSGMRFLSS 373
>At5g23550 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 175
Score = 59.7 bits (143), Expect = 3e-09
Identities = 31/107 (28%), Positives = 57/107 (52%), Gaps = 3/107 (2%)
Query: 96 CVFLFPVLAVQPRKFGLLWSMGSLLFIFAFGVLQGPVAYMRHLTSRERLPFTVFFFVSCF 155
C L ++ P KFG+ +++G+L+ + + L GP + + R+ T + S
Sbjct: 63 CTLLSMLVFFNPVKFGITFTLGNLMALGSTAFLIGPQRQVTMMLDPARIYATALYLASII 122
Query: 156 LTIYFAAFTKSTLLTIPCAILELIAVIYYAISYFPFGGTGLRMISSI 202
+ ++ A + ++ LLT+ ILE +I+Y++SY PF T M+S I
Sbjct: 123 IALFCALYVRNKLLTLLAIILEFTGLIWYSLSYIPFART---MVSKI 166
>Hs14736892 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 160
Score = 50.1 bits (118), Expect = 2e-06
Identities = 35/126 (27%), Positives = 62/126 (48%), Gaps = 7/126 (5%)
Query: 86 ILGAVACFTI---CVFLFPVLAVQPRK----FGLLWSMGSLLFIFAFGVLQGPVAYMRHL 138
I G +ACF I C L VL PRK F + ++ G++ I + L GPV ++ +
Sbjct: 34 IKGFIACFAIGILCSLLGTVLLWVPRKGLHLFAVFYTFGNIASIGSTIFLMGPVKQLKRM 93
Query: 139 TSRERLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILELIAVIYYAISYFPFGGTGLRM 198
RL T+ + LT+ A + + L + IL+ +A+ +Y++S+ PF ++
Sbjct: 94 FEPTRLIATIMVLLCFALTLCSAFWWHNKGLALIFCILQSLALTWYSLSFIPFARDAVKK 153
Query: 199 ISSIGL 204
++ L
Sbjct: 154 CFAVCL 159
>7296331 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 163
Score = 49.3 bits (116), Expect = 4e-06
Identities = 34/133 (25%), Positives = 67/133 (49%), Gaps = 7/133 (5%)
Query: 72 SLSRTERLSLFV-CFILGAVACF--TICVFLFPVLAVQPRKFGLLWSMGSLLFIFAFGVL 128
+LS + R+ FV CF LG + ++ +FL + V F + +++G+++ + + L
Sbjct: 30 TLSWSTRIKGFVICFALGILLSLLGSVALFLHRGIVV----FAVFYTLGNVISMASTCFL 85
Query: 129 QGPVAYMRHLTSRERLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILELIAVIYYAISY 188
GP ++ + + RL T+ V LT A K LT+ I++ +A+ +Y++SY
Sbjct: 86 MGPFKQIKKMFAETRLIATIIVLVMMVLTFIAAIVWKKAGLTLIFIIIQSLAMTWYSLSY 145
Query: 189 FPFGGTGLRMISS 201
P+ ++ S
Sbjct: 146 IPYARDAVKKTMS 158
>ECU09g0940 [U] KOG2887 Membrane protein involved in ER to Golgi transport
Length = 171
Score = 48.9 bits (115), Expect = 5e-06
Identities = 29/120 (24%), Positives = 61/120 (50%), Gaps = 9/120 (7%)
Query: 92 CFTICV------FLFPVLAV---QPRKFGLLWSMGSLLFIFAFGVLQGPVAYMRHLTSRE 142
CF+ C+ FL+ ++ + P F L +++ + LF FG L G +Y+ L S++
Sbjct: 48 CFSACLGAGVLSFLYSMIKIVRLSPSGFILPYTISNFLFFIMFGFLLGFRSYLEGLFSKK 107
Query: 143 RLPFTVFFFVSCFLTIYFAAFTKSTLLTIPCAILELIAVIYYAISYFPFGGTGLRMISSI 202
+ + +F LT+Y LL + +++++ I +++++ P G +G+ I S+
Sbjct: 108 KRVHSSWFIGCTLLTLYVVLKYDRYLLNLAFCFIQVVSFIMFSLTFIPGGTSGVSSIISM 167
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.329 0.141 0.440
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,210,605
Number of Sequences: 60738
Number of extensions: 470855
Number of successful extensions: 1562
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1553
Number of HSP's gapped (non-prelim): 10
length of query: 213
length of database: 30,389,216
effective HSP length: 102
effective length of query: 111
effective length of database: 24,193,940
effective search space: 2685527340
effective search space used: 2685527340
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)