ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1702 good G KOG4125 Carbohydrate transport and metabolism Acid trehalase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1702 601655 598215 -1147
(1147 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR026w [G] KOG4125 Acid trehalase 1238 0.0
7297891 [G] KOG4125 Acid trehalase 159 2e-38
Hs13376652 [G] KOG4125 Acid trehalase 124 9e-28
>YPR026w [G] KOG4125 Acid trehalase
Length = 1211
Score = 1238 bits (3202), Expect = 0.0
Identities = 653/1192 (54%), Positives = 830/1192 (68%), Gaps = 81/1192 (6%)
Query: 2 LIVPFILFSVAILAPIYFYLTKPLPLQHTHFG-----------SFVCAEDTLFCPESQRK 50
+++ +LF +AI YLTK L ++H S TL R+
Sbjct: 51 ILLLMMLFLLAIFVTA-LYLTKSSRLTYSHASRAALFNPLGVISPSLGNHTLNYDPEARE 109
Query: 51 ASEKMYSLLKDHENTFYDEEQQILGNLLLSENTFSRQPYVANGYIGSRIPNLGFGYALDT 110
+S+K+Y LL D +YD+E ILG+ L S+NT+SRQPYVANGYIGSRIPN+GFGYALDT
Sbjct: 110 SSKKLYELLSDFNTAYYDDENMILGSNLFSKNTYSRQPYVANGYIGSRIPNIGFGYALDT 169
Query: 111 INVWVNDSSIPGALDNGWPLRNQRFAGAFISDFYCLQEKLNSTNFPELDDDGYSTVISTI 170
+N + + PGAL+NGWPLRN RFAGAF+SDFYCLQ KLNSTNFPELDD GYSTVIS+I
Sbjct: 170 LNFYTD---APGALNNGWPLRNHRFAGAFVSDFYCLQPKLNSTNFPELDDVGYSTVISSI 226
Query: 171 PQWTDLSILKHTTTGQIEYINPTDVKLDKITNYMQNLSLQDGIVTTTFVY-DKQLLVTTK 229
PQWT+L + ++ NP +V LD +TNY QNLS++DGIVTT + + Q+ V ++
Sbjct: 227 PQWTNLQF---SLVNDSKWFNPQNVTLDDVTNYSQNLSMKDGIVTTELDWLNSQIHVKSE 283
Query: 230 VVAHRKIYPLGVVTLELSLFDNNSDTSADNENEYVELEICDSLNFSTSHRTVLNDYGYDQ 289
+ AHR I+PLGVV+LE+SL ++ + D+ +++ I D L+F+TSHRTVL+ G D+
Sbjct: 284 IWAHRHIHPLGVVSLEISLNTDHLPSDFDS----LDVNIWDILDFNTSHRTVLHSTGTDE 339
Query: 290 NNEGIFMVVEPENVPYSNASLFSYFDIPSRDTLTLSKYSDSISQCTTQ-------ILKAN 342
N +FM+V+P+NVP SN +++S + ++ S+S + + +
Sbjct: 340 KNNAVFMIVQPDNVPSSNCAIYSTCTVKYENSTNPINSSESFEEKDVSSNIYNVILTEDQ 399
Query: 343 STFVAHKYIGIISSEYD-NKQPDDNSNGTSNEALKMSNLERATSIVLDNKGNYDSLIQSH 401
+ HKY+GI+S+E++ NK+ DN+N + A I L++KGNY+ L+ SH
Sbjct: 400 PKIIVHKYVGIMSTEFNKNKEQQDNTN-----------IGLAKMIALNSKGNYEKLLSSH 448
Query: 402 KNAWKRIYKDASIEIPSDGLLEMTAKSSIYHLLANTRSHNVSEDRGLPIGVSGLSSDSYG 461
K AW +Y DA IEIPSD LLEMTA+SS++HLLANTR +NVS DRGLP+GVSGLSSDSYG
Sbjct: 449 KRAWYDLYNDAFIEIPSDSLLEMTARSSLFHLLANTRDYNVSSDRGLPVGVSGLSSDSYG 508
Query: 462 GMVFWDSDLWILPALLPFFPNAARQINNYRNASLHQAKLNAEKYGYDGALYPWTSGRYAN 521
GMVFWD+D+W+ PALLPFFPN A+ +NNYRNA+ QAKLNAEKYGY GA+YPWTSG+YAN
Sbjct: 509 GMVFWDADIWMEPALLPFFPNVAQNMNNYRNATHSQAKLNAEKYGYPGAIYPWTSGKYAN 568
Query: 522 CTSTGPCVDYEYHINVDIALSSFAIYMNGDEDDERSEEYLRYTTWPFVENAAKMFAQYVK 581
CTSTGPCVDYEYHINVD+A++SF+IY+NG E + +EYLRYTTWP ++NAA+ F YVK
Sbjct: 569 CTSTGPCVDYEYHINVDVAMASFSIYLNGHEGID--DEYLRYTTWPIIKNAAQFFTAYVK 626
Query: 582 WNDTMQQYTTHNLTDPDEYANFVDNAAFTNAGIQSVMVWAHDIARHLGIDPDPQWLEIAD 641
+N ++ Y T+NLTDPDE+AN ++N AFTNAGI++++ WA DI HLG DP+W EI+
Sbjct: 627 YNSSLGLYETYNLTDPDEFANHINNGAFTNAGIKTLLKWATDIGNHLGEVVDPKWSEISK 686
Query: 642 NIHIPISETNITLEYSGMNSSVEIKQADVVLMIYPLSYITDQSILNNAIKNLYYYSERQS 701
+I+IP S +NITLEYSGMNSSVEIKQADV LM+YPL YI D+SILNNAIK+LYYYSERQS
Sbjct: 687 DIYIPRSSSNITLEYSGMNSSVEIKQADVTLMVYPLGYINDESILNNAIKDLYYYSERQS 746
Query: 702 ASGPAMTYPVFVAGAASLLNYGSSSQSYLYKSVVPYLRSPFAQFSEQSDDNFLTNGLTQP 761
ASGPAMTYPVFVA AA LLN+GSSSQSYLYKSV+PYLR+PFAQFSEQSDDNFLTNGLTQP
Sbjct: 747 ASGPAMTYPVFVAAAAGLLNHGSSSQSYLYKSVLPYLRAPFAQFSEQSDDNFLTNGLTQP 806
Query: 762 AFPFLTANGGFLQSILFGLTGLRYSYEVDEDTGKIHRLLKFNPIQLPLLPGGIRINNFKY 821
AFPFLTANGGFLQSILFGLTG+RYSYEVD DT KI+RLL+FNPI+LPLLPGGI I NFKY
Sbjct: 807 AFPFLTANGGFLQSILFGLTGIRYSYEVDPDTKKINRLLRFNPIELPLLPGGIAIRNFKY 866
Query: 822 MGQVLDVLITDTEGIIIHKNGTKEIRIKVPDRTLIPDVDVKYSEETDPIKQILHGRRSVP 881
M QVLD++I D G I+HK+G I IK+P+R+LI D D+ + ++ ++ RR V
Sbjct: 867 MNQVLDIIIDDHNGTIVHKSGDVPIHIKIPNRSLIHDQDINFYNGSENERKPNLERRDVD 926
Query: 882 TGKN---------YFTIQPGDVFKTPLYIPKKNLEGNLVEAKQITNLTAGVPGDVAVSVI 932
+ Y+ ++P L+ P N N+ E KQITNLTAGVPGDVA S +
Sbjct: 927 RVGDPMRMDRYGTYYLLKPKQELTVQLFKPGLNARNNIAENKQITNLTAGVPGDVAFSAL 986
Query: 933 DGNNFTHWQPAYKNLPARLLIDMGNNFTQEIKSGKIIWGSRPAKTFSLSILPQTEEV--- 989
DGNN+THWQP K A+LLID+G +EI G I+WG RPAK S+SILP +E+V
Sbjct: 987 DGNNYTHWQPLDKIHRAKLLIDLGEYNEKEITKGMILWGQRPAKNISISILPHSEKVENL 1046
Query: 990 FKNLTHILSN----------VNQYCNKTG--DECVRDLERTEKGFDAAIEDV---FDWYG 1034
F N+T I+ N + Q + G E V D + E+ D +++DV W
Sbjct: 1047 FANVTEIMQNSGNDQLLNETIGQLLDNAGIPVENVIDFDGIEQEDDESLDDVQALLHWKK 1106
Query: 1035 IDPDSIISTHPELKDMKTKFVKILDHYKVTPSEPYPWRVYNESQIVLLPGNETDFDIDYS 1094
D +I P L +K KFVKILD+ V+PSEPY N+S I +LP N T F IDY
Sbjct: 1107 EDLAKLIEQIPRLNFLKRKFVKILDNVPVSPSEPYYEASRNQSLIEILPSNRTTFTIDYD 1166
Query: 1095 KVAEMNPENVDIDFRGNQTDWRKGRFIVLTVHDTYXXXXXE-KGATIKEFAL 1145
K + + +GN TDWRK R+IV+ V Y + KGATIKE L
Sbjct: 1167 K--------LQVGDKGN-TDWRKTRYIVVAVQGVYDDYDDDNKGATIKEIVL 1209
>7297891 [G] KOG4125 Acid trehalase
Length = 690
Score = 159 bits (403), Expect = 2e-38
Identities = 121/413 (29%), Positives = 197/413 (47%), Gaps = 34/413 (8%)
Query: 395 DSLIQSHKNAWKRIYKDA-SIEIPSDGL-LEMTAKSSIYHLLANTRSHNVSEDRGLPIGV 452
D+L+Q H +W + D SIEI D L L + I++++++ S ++ L G+
Sbjct: 223 DNLLQKHSQSWNSFWDDEFSIEIKGDALELSQIVNAGIFYIVSSLPSLETNQKNELFYGL 282
Query: 453 S------GLSSDSYGGMVFWDSDLWILPALLPFFPNAARQINNYRNASLHQAKLNAEKYG 506
S G Y G FWD+++W+LP + F + A+++ YR+ L AK +A G
Sbjct: 283 SPTGLGRGNLEADYQGHNFWDTEIWMLPIVSLFGLDYAKEVLGYRSRKLEAAKYHANATG 342
Query: 507 YDGALYPWTSGRYANCTSTGPC----VDYEYHINVDIALSSFAIYMNGDEDDERSEEYLR 562
Y GA +PW S Y T PC + E HI+ DI+ + + ++ E+
Sbjct: 343 YKGARFPWESA-YTGTEVTNPCCPEVAEQEIHISPDISFALQKFFAQSND-----SEWTC 396
Query: 563 YTTWPFVENAAKMFAQYVKWNDTMQQYTTHNLTDPDEYANFVDNAAFTNAGIQSVMVWAH 622
T WP + A +D+ + N+ PDE V++ +TNA + + +A
Sbjct: 397 GTAWPITADVADFLVSRATCDDSTKTCHLLNVMGPDEDHPNVNDNVYTNAVSKIALNFAE 456
Query: 623 DIARHLG---IDPDPQWLEIADNIHIPISET-NITLEYSGMNSSVEIKQADVVLMIYPLS 678
+A+H G + +W E++D + I E+ N EY+G IKQAD +L+ YPL+
Sbjct: 457 WLAKHCGNASTERLEKWREVSDGLVILRDESLNYHPEYAGYVRDTIIKQADTILLGYPLN 516
Query: 679 YITDQSILNNAIKNLYYYSERQSASGPAMTYPVFVAGAASLLNYGSSSQSYLYKSVVPYL 738
+ D+S + I +L +Y+ SGPAMT+ +F A L + + Y + Y+
Sbjct: 517 F--DKS--STHINDLRFYANVTRESGPAMTWSMFAANYLRNLQL-TLANEYFERGYKSYV 571
Query: 739 RSPFAQFSEQSDDNFLTNGLTQPAFPFLTANGGFLQSILFGLTGLRYSYEVDE 791
R F +SE T + FLT GGFLQ+++FG GL + D+
Sbjct: 572 RPEFKVWSE-------TPIGYDGSANFLTGIGGFLQALIFGYGGLDFGRTDDD 617
>Hs13376652 [G] KOG4125 Acid trehalase
Length = 323
Score = 124 bits (311), Expect = 9e-28
Identities = 77/260 (29%), Positives = 127/260 (48%), Gaps = 17/260 (6%)
Query: 396 SLIQSHKNAWKRIYKDASIEIPSDGLLEMTAKSSIYHLLANTRSHNVSEDRGLPIGVSGL 455
+L +H AW +++ + +++ L + S+Y+LL+ + GL
Sbjct: 49 ALYTAHAQAWAQLWVECGLDVVGPLQLRQALRGSLYYLLSALPQPKAPGYICHGLSPGGL 108
Query: 456 SSDS----YGGMVFWDSDLWILPALLPFFPNAARQINNYRNASLHQAKLNAEKYGYDGAL 511
S+ S Y G VFWD DLW+ P++L F P AAR I YR +L A NA+ GY GA
Sbjct: 109 SNGSREECYWGHVFWDQDLWMFPSILMFHPEAARAILEYRIRTLDGALENAQNLGYQGAK 168
Query: 512 YPWTSGRYANCTSTGPCVD-----YEYHINVDIALSSFAIYMNGDEDDERSEEYLRYTTW 566
+ W S + C + E H+N + L +F +Y + +D + E W
Sbjct: 169 FAWESAD----SGLEVCPEDIYGVQEVHVNGAVVL-AFELYYHTTQDLQLFRE---AGGW 220
Query: 567 PFVENAAKMFAQYVKWNDTMQQYTTHNLTDPDEYANFVDNAAFTNAGIQSVMVWAHDIAR 626
V A+ + V+W+ ++Y + PDEY + V+N+ +TN +Q+ + +A +A+
Sbjct: 221 DVVRAVAEFWCSRVEWSPREEKYHLRGVMSPDEYHSGVNNSVYTNVLVQNSLRFAAALAQ 280
Query: 627 HLGIDPDPQWLEIADNIHIP 646
LG+ QWL +AD I +P
Sbjct: 281 DLGLPIPSQWLAVADKIKVP 300
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,008,308
Number of Sequences: 60738
Number of extensions: 3347374
Number of successful extensions: 7729
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 7709
Number of HSP's gapped (non-prelim): 3
length of query: 1147
length of database: 30,389,216
effective HSP length: 117
effective length of query: 1030
effective length of database: 23,282,870
effective search space: 23981356100
effective search space used: 23981356100
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)