ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1808 suspect: HSP O KOG2164 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1808 641391 642227 279
(279 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER116c [O] KOG2164 Predicted E3 ubiquitin ligase 105 5e-23
Hs7662653 [O] KOG2164 Predicted E3 ubiquitin ligase 54 2e-07
At5g05130 [KL] KOG1001 Helicase-like transcription factor HLTF/D... 53 4e-07
SPBC3D6.11c [O] KOG0317 Predicted E3 ubiquitin ligase integral p... 50 4e-06
At5g22750 [KL] KOG1001 Helicase-like transcription factor HLTF/D... 50 5e-06
7292099 [O] KOG2164 Predicted E3 ubiquitin ligase 50 5e-06
>YER116c [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 274
Score = 105 bits (263), Expect = 5e-23
Identities = 54/101 (53%), Positives = 68/101 (66%), Gaps = 7/101 (6%)
Query: 177 QQVIEIDDEDETEGHKSGKDMSETPLETKKAADYVCPICMEPPEAALVTKCGHVFCTTCL 236
QQV++I D+D E K E P E A DY CPIC EPPE AL+T CGHVFC CL
Sbjct: 177 QQVLQISDDDFQEETK------EAPKEYGAAKDYRCPICFEPPETALMTLCGHVFCCPCL 230
Query: 237 YGMVNSSKGNGRRNGLCALCRENVKLQDLRLIVMRKNRIRK 277
+ MVNSS+ R+ G CALCR V L+D+RLI++RK +++K
Sbjct: 231 FQMVNSSR-TCRQFGHCALCRSKVYLKDVRLIILRKKQVKK 270
>Hs7662653 [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 761
Score = 54.3 bits (129), Expect = 2e-07
Identities = 22/58 (37%), Positives = 36/58 (61%), Gaps = 3/58 (5%)
Query: 212 CPICMEPPEAALVTKCGHVFCTTCLYGMVNSSKGNGRRNGLCALCRENVKLQDLRLIV 269
CPIC+ PP AA +T+CGH+FC C+ ++ S+ + C +C +V +DL+ +V
Sbjct: 175 CPICLYPPTAAKITRCGHIFCWACILHYLSLSEKTWSK---CPICYSSVHKKDLKSVV 229
>At5g05130 [KL] KOG1001 Helicase-like transcription factor HLTF/DNA helicase
RAD5 DEAD-box superfamily
Length = 881
Score = 53.1 bits (126), Expect = 4e-07
Identities = 22/57 (38%), Positives = 32/57 (55%), Gaps = 6/57 (10%)
Query: 209 DYVCPICMEPPEAALVTKCGHVFCTTCLYGMVNSSKGNGRRNGLCALCRENVKLQDL 265
D+ CPIC+ PP ++T+C H+FC C+ + SK LC LCR ++ DL
Sbjct: 632 DFDCPICISPPTNIIITRCAHIFCRACILQTLQRSK------PLCPLCRGSLTQSDL 682
>SPBC3D6.11c [O] KOG0317 Predicted E3 ubiquitin ligase integral peroxisomal
membrane protein
Length = 269
Score = 50.1 bits (118), Expect = 4e-06
Identities = 36/116 (31%), Positives = 48/116 (41%), Gaps = 27/116 (23%)
Query: 168 LTEEEQIKAQ-----QVIEIDDEDETEGHKS----GKDMSETPLETKKA----------- 207
LT I AQ +I++ DE + K GK+ S T E +K
Sbjct: 139 LTNVNSIHAQPTTISDMIDLTDETSYDPRKQKFEQGKNPSTTNAEIEKEEPSKKQVVPSS 198
Query: 208 ---ADYVCPICMEPPEAALVTKCGHVFCTTCLYGMVNSSKGNGRRNGLCALCRENV 260
ADY C IC++ PE T CGH+FC C+ S+ G C +CR V
Sbjct: 199 QRLADYKCVICLDSPENLSCTPCGHIFCNFCIL----SALGTTAATQKCPVCRRKV 250
>At5g22750 [KL] KOG1001 Helicase-like transcription factor HLTF/DNA helicase
RAD5 DEAD-box superfamily
Length = 1029
Score = 49.7 bits (117), Expect = 5e-06
Identities = 28/80 (35%), Positives = 38/80 (47%), Gaps = 11/80 (13%)
Query: 192 KSGKDM------SETPLETKKAADYVCPICMEPPEAALVTKCGHVFCTTCLYGMVNSSKG 245
+ GKD+ E E +K CPIC+E E A++T C H C CL +S
Sbjct: 768 REGKDVPSEAFVQEVVEELRKGEQGECPICLEALEDAVLTPCAHRLCRECLLASWRNS-- 825
Query: 246 NGRRNGLCALCRENVKLQDL 265
+GLC +CR V Q+L
Sbjct: 826 ---TSGLCPVCRNTVSKQEL 842
>7292099 [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 708
Score = 49.7 bits (117), Expect = 5e-06
Identities = 22/63 (34%), Positives = 35/63 (54%), Gaps = 3/63 (4%)
Query: 212 CPICMEPPEAALVTKCGHVFCTTCLYGMVNSSKGNGRRNGLCALCRENVKLQDLRLIVMR 271
CPIC+ PP AA +T+CGH +C CL ++ S R+ C +C + + DL+ +
Sbjct: 212 CPICLYPPVAAKLTRCGHAYCWPCLLHYLSLSDKTWRK---CPICYDAIHAGDLKSCTIE 268
Query: 272 KNR 274
+ R
Sbjct: 269 QLR 271
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.311 0.130 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,824,196
Number of Sequences: 60738
Number of extensions: 554555
Number of successful extensions: 2782
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2779
Number of HSP's gapped (non-prelim): 6
length of query: 279
length of database: 30,389,216
effective HSP length: 105
effective length of query: 174
effective length of database: 24,011,726
effective search space: 4178040324
effective search space used: 4178040324
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)