ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV1883 good L KOG3108 Replication, recombination and repair Single-stranded DNA-binding replication protein A (RPA), medium (30 kD) subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV1883 668590 667910 -227
(227 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL312w [L] KOG3108 Single-stranded DNA-binding replication prot... 249 2e-66
SPCC1753.01c [L] KOG3108 Single-stranded DNA-binding replication... 80 2e-15
At2g24490 [L] KOG3108 Single-stranded DNA-binding replication pr... 67 2e-11
Hs9558731 [L] KOG3108 Single-stranded DNA-binding replication pr... 57 2e-08
Hs4506585 [L] KOG3108 Single-stranded DNA-binding replication pr... 57 3e-08
At3g02920 [L] KOG3108 Single-stranded DNA-binding replication pr... 54 2e-07
7298736 [L] KOG3108 Single-stranded DNA-binding replication prot... 49 6e-06
>YNL312w [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 273
Score = 249 bits (636), Expect = 2e-66
Identities = 120/227 (52%), Positives = 162/227 (70%), Gaps = 6/227 (2%)
Query: 4 AKQQIQDGPYVIMGQELHHVSFVGVIRNVADNTSNVTLTVEDGTGQIEFRKWTNDSNDMS 63
+KQ IQDGP+V QELHHV FVGV+RN+ D+T+N+ LT+EDGTGQIE RKW+ D+ND++
Sbjct: 50 SKQDIQDGPFVSHNQELHHVCFVGVVRNITDHTANIFLTIEDGTGQIEVRKWSEDANDLA 109
Query: 64 HASQEDQNGDYNSQVAQDYSVGKYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAHHLE 123
+ + Y SQVAQ + +G Y+KV+ +L+EF GK N+QYAV+K IDSFNE+L HHLE
Sbjct: 110 AGNDDSSGKGYGSQVAQQFEIGGYVKVFGALKEFGGKKNIQYAVIKPIDSFNEVLTHHLE 169
Query: 124 VIKAFAVANGNLSVDSTVAVNGANTETNSLFVQDNEPPKSAT---EKVLDFIRAQCAGKD 180
VIK ++A+G + A +N SLFV+D+ S + +++L+F + QC GKD
Sbjct: 170 VIKCHSIASGMMKQPLESA---SNNNGQSLFVKDDNDTSSGSSPLQRILEFCKKQCEGKD 226
Query: 181 SNSFSVQTKLISQSLNMIEDDVRMYCQTLTEQGYIYPTFDEFTFFIL 227
+NSF+V LISQSLN+ E VR C TLT+QG+IYPTFD+ FF L
Sbjct: 227 ANSFAVPIPLISQSLNLDETTVRNCCTTLTDQGFIYPTFDDNNFFAL 273
>SPCC1753.01c [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 247
Score = 80.1 bits (196), Expect = 2e-15
Identities = 65/233 (27%), Positives = 107/233 (45%), Gaps = 31/233 (13%)
Query: 3 SAKQQIQDGPYVIMGQELHHVSFVGVIRNVADNTSNVTLTVEDGTGQIEFRKWTNDSNDM 62
+A Q D + I G E+ V+FVGV+RN+ T+N T +EDGTG IE R W
Sbjct: 28 NASQVHADAEFKIDGVEVGQVTFVGVLRNIHAQTTNTTYQIEDGTGMIEVRHW------- 80
Query: 63 SHASQEDQNGDYNSQVAQDYSVGKYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAHHL 122
++ D S++A D Y++VY +++ FSGK+ + ++ I NE+ H L
Sbjct: 81 -------EHIDALSELATD----TYVRVYGNIKIFSGKIYIASQYIRTIKDHNEVHFHFL 129
Query: 123 EVI--------KAFAVANGN---LSVDSTVAVNGANTETNSLFVQDNEPPKSATEKVLDF 171
E I KA AV N + + N ++ + ++ S T +
Sbjct: 130 EAIAVHLHFTQKANAVNGANAPGYGTSNALGYNNISSNGAANSLEQKLAEYSLTPAQMTV 189
Query: 172 IRAQCAGKDSNSFSVQTKLISQSLNMIEDDVRMYCQTLTEQGYIYPTFDEFTF 224
++A + ++N V + ++QS+ D+ L ++G IY T DE F
Sbjct: 190 MQAIHSAPETNE-GVHVRQLAQSVGP-GIDLTAVTDFLQQEGIIYTTIDENHF 240
>At2g24490 [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 250
Score = 67.4 bits (163), Expect = 2e-11
Identities = 50/215 (23%), Positives = 102/215 (47%), Gaps = 37/215 (17%)
Query: 14 VIMGQELHHVSFVGVIRNVADN-TSNVTLTVEDGTGQIEFRKWTNDSNDMSHASQEDQNG 72
VI G L +VS VG++ + ++ + V T++DGTG+I+ ++W +++
Sbjct: 64 VINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSET------------- 110
Query: 73 DYNSQVAQDYSVGKYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAHHLEVIKAFAVAN 132
++++ + G Y+++ L+ F GK + V+ I FNE+ H++E I ++
Sbjct: 111 -FDAREMESVRDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYS--- 166
Query: 133 GNLSVDSTVAVNGANTETNSLFVQDNE--PPKSATEKVLDFIR-AQCAGKDSNSFSVQTK 189
N+E+ + Q+N+ K+ + +LD+++ C + +
Sbjct: 167 -------------QNSESQVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQ---GIHID 210
Query: 190 LISQSLNMIEDDVRMYCQTLTEQGYIYPTFDEFTF 224
I+Q L + ++ + Q+L G IY T DE+ F
Sbjct: 211 EIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHF 245
>Hs9558731 [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 261
Score = 57.4 bits (137), Expect = 2e-08
Identities = 52/225 (23%), Positives = 96/225 (42%), Gaps = 22/225 (9%)
Query: 2 QSAKQQIQDGPYVIMGQELHHVSFVGVIRNVADNTSNVTLTVEDGTGQ-IEFRKWTNDSN 60
Q + D + + G + VS VGVIR ++++ ++D T + IE R+W
Sbjct: 53 QLLSSTVFDPVFKVRGIIVSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWF---- 108
Query: 61 DMSHASQEDQNGDYNSQVAQDYSVGKYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAH 120
G + SVG Y+KV+ L+ +G +++ + ++ NE H
Sbjct: 109 -----------GREKVKQVTPLSVGVYVKVFGILKCPTGTKSLEVLKIHVLEDMNEFTVH 157
Query: 121 HLEVIKAFAVANGNLSVDSTVAVNGANTETNSLFVQDNEPPKSATEKVLDFIRAQCAGKD 180
LE + A + + + +V + +E N D ++VL I +C ++
Sbjct: 158 ILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIH-ECPHQE 216
Query: 181 SNSF-SVQTKLISQSLNMIEDDVRMYCQTLTEQGYIYPTFDEFTF 224
S ++ +L S+ I++ + LT +G+IYPT D F
Sbjct: 217 GKSIHELRAQLCDLSVKAIKEAI----DYLTVEGHIYPTVDREHF 257
>Hs4506585 [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 270
Score = 56.6 bits (135), Expect = 3e-08
Identities = 55/222 (24%), Positives = 91/222 (40%), Gaps = 25/222 (11%)
Query: 10 DGPYVIMGQELHHVSFVGVIRNVADNTSNVTLTVEDGTGQ-IEFRKWTNDSNDMSHASQE 68
D + I E+ V+ VG+IR+ +N+ ++D T ++ R+W +
Sbjct: 61 DEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDT---------- 110
Query: 69 DQNGDYNSQVAQDYSVGKYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAHHLEVIKAF 128
D N+ V + Y+KV LR F K ++ + ++ NE H LEVI A
Sbjct: 111 DDTSSENTVVPPE----TYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAH 166
Query: 129 AV---ANGNLSVDSTVAVNGANTETNSLFVQDNEPPKSAT---EKVLDFIRAQCAGKDSN 182
V AN S N +E + P T +VL+ I+A C +
Sbjct: 167 MVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKA-CPRPEGL 225
Query: 183 SFSVQTKLISQSLNMIEDDVRMYCQTLTEQGYIYPTFDEFTF 224
+F L +Q +M ++ L+ +G+IY T D+ F
Sbjct: 226 NFQ---DLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHF 264
>At3g02920 [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 282
Score = 53.5 bits (127), Expect = 2e-07
Identities = 54/233 (23%), Positives = 97/233 (41%), Gaps = 37/233 (15%)
Query: 13 YVIMGQELHHVSFVGVIRNVADNTSNVTLTVEDGTGQIEFRKWTNDSNDMSHASQEDQNG 72
+ I G ++ V VG I + + + V V+DGTG ++ +W HA QE +
Sbjct: 60 FSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWC-------HARQETEE- 111
Query: 73 DYNSQVAQDYSVGKYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAHHLEVIKAF---- 128
+ +G Y++++ L+ F GK +V V+ + FNEI+ H E +
Sbjct: 112 ------MEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNT 165
Query: 129 AVANGNLSVD----------STVAVNGANTET----NSLFV---QDNEPPKSATEKVLDF 171
+ G+++ D ST+ +T +LF Q N+ + VL++
Sbjct: 166 KLRGGSITQDTATPRPQMPYSTMPTPAKPYQTGPSNQNLFQFPNQFNDSMHGVKQTVLNY 225
Query: 172 IRAQCAGKDSNSFSVQTKLISQSLNMIEDDVRMYCQTLTEQGYIYPTFDEFTF 224
+ + V +I++ L + V+ + L+ G IY T DE F
Sbjct: 226 LNQPM--HIVSEAGVHCDIIARELRIPLLQVKEALEQLSNDGCIYSTLDETCF 276
>7298736 [L] KOG3108 Single-stranded DNA-binding replication protein A (RPA)
medium (30 kD) subunit
Length = 195
Score = 48.9 bits (115), Expect = 6e-06
Identities = 32/128 (25%), Positives = 57/128 (44%), Gaps = 15/128 (11%)
Query: 26 VGVIRNVADNTSNVTLTVEDGTGQIEFRKWTNDSNDMSHASQEDQNGDYNSQVAQDYSVG 85
V ++RNV +++ +T T+ED +G+I+ W + + + A + V
Sbjct: 5 VAIVRNVETSSTKITYTLEDHSGRIDAHYWLEEGDALK---------------APEVMVN 49
Query: 86 KYIKVYASLREFSGKMNVQYAVVKHIDSFNEILAHHLEVIKAFAVANGNLSVDSTVAVNG 145
Y+KVY + R G + + + NE+ H LEV+ A A S A G
Sbjct: 50 NYVKVYGTTRSQGGSKTLMIFKLLPVLDPNEVCTHLLEVLNARYRAEDYQSKGGAGAGAG 109
Query: 146 ANTETNSL 153
A++ + S+
Sbjct: 110 ASSGSGSI 117
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.130 0.363
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,105,178
Number of Sequences: 60738
Number of extensions: 521996
Number of successful extensions: 1575
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1562
Number of HSP's gapped (non-prelim): 8
length of query: 227
length of database: 30,389,216
effective HSP length: 103
effective length of query: 124
effective length of database: 24,133,202
effective search space: 2992517048
effective search space used: 2992517048
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)