ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV1941    suspect: LH   O     KOG0826  Posttranslational modification, protein turnover, chaperones   Predicted E3 ubiquitin ligase involved in peroxisome organization

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV1941 685564  686556 331  
         (331 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR026c [O] KOG0826 Predicted E3 ubiquitin ligase involved in pe...   112   1e-24

>YMR026c [O] KOG0826 Predicted E3 ubiquitin ligase involved in peroxisome
           organization
          Length = 399

 Score =  112 bits (279), Expect = 1e-24
 Identities = 93/373 (24%), Positives = 164/373 (43%), Gaps = 56/373 (15%)

Query: 1   MDFYSNLP----------------VNLQQ--PTLFEILSVNEVKKLIKPTLRYIFSIYLQ 42
           M FYSNLP                V L+   PT+FEI+S  E+  L+  ++RY+ + +L 
Sbjct: 1   MSFYSNLPSAGQSSRGSSTSGRNGVGLEPLYPTIFEIMSSQEIDSLLPASIRYLLANHLV 60

Query: 43  YRGPTRWLLKIFNKFDFIILVIKSLVEYRHYKTTGATILDKFYGLKRF------------ 90
              P R+ L++   F      IK  VE+ H KT  +T +D+FYGL+ F            
Sbjct: 61  ANFPNRYTLRLNKYFFEWFQAIKGFVEWYHLKTYNSTFIDRFYGLQLFSSRDRNLALTQC 120

Query: 91  ------SRFPKLTFLG------IWLNDCLFEYVSDICEQYHELLQSRKLTSPE--LSSW- 135
                 S +P+   L       I+L   +  Y++   ++  E +    + S +   + W 
Sbjct: 121 LNPKGQSEWPQGLQLNQQQKSVIFLEKIILPYITAKLDEILEKISMNNIFSSDETENKWP 180

Query: 136 QQWFDAYYPKLQKTIKVINFCFKLKYL-RHSKDTDMIHFITQIRYQRYQEPEEGIASRKN 194
           ++ F   YP ++K + + N   KL +L + +    ++ ++ +I Y   +     ++  K 
Sbjct: 181 KRAFLRIYPFIKKLLALSNLLVKLLFLTKRTGSVSLLQYLFKIEYTTVRPLSSELSGLKE 240

Query: 195 TLTLSERRRKRTNLPRILAMTK---DAVESTSTMFLDKLFPSFLVMIRILQIINQRPELF 251
           T  +  R RK TN+  I A+ +     +    T    + FP+F+ ++R+ Q    +    
Sbjct: 241 TKGMDNRLRK-TNISSIFALMQGQLSIIPRFLTFMGSQFFPTFIFVLRVYQWWTTQDMTT 299

Query: 252 KKEIRVKR-----PKPPVLPGVASEVDNNDTTDVCPLCGEEITEPAMISSGYVANLECAK 306
           K + RV       P+PP         D    ++ CP+C + +  P ++ +GYVA   CA 
Sbjct: 300 KLQKRVNDLDEDIPRPPFSSHSDKTEDKEGVSEACPVCEKTVQNPCVLETGYVACYPCAI 359

Query: 307 KW-VSTENTCFAT 318
            + V+ E  C  T
Sbjct: 360 SYLVNNEGHCPVT 372


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.324    0.140    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,347,839
Number of Sequences: 60738
Number of extensions: 864255
Number of successful extensions: 3141
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3139
Number of HSP's gapped (non-prelim): 1
length of query: 331
length of database: 30,389,216
effective HSP length: 107
effective length of query: 224
effective length of database: 23,890,250
effective search space: 5351416000
effective search space used: 5351416000
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)