ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV1948    suspect: LH   J     KOG3769  Translation, ribosomal structure and biogenesis                Ribonuclease III domain proteins

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV1948 688389 687445 -315 
         (315 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR024w [J] KOG3769 Ribonuclease III domain proteins                  293   2e-79
SPBC3B9.14c [J] KOG3769 Ribonuclease III domain proteins              122   5e-28

>YMR024w [J] KOG3769 Ribonuclease III domain proteins
          Length = 390

 Score =  293 bits (750), Expect = 2e-79
 Identities = 168/329 (51%), Positives = 220/329 (66%), Gaps = 37/329 (11%)

Query: 21  LYQSELSSLKEY----KNTISTLLKNPAHELTSPNLVALHSRLRLPQGXXXXXXXXXXXX 76
           L +SEL+  KEY    K+T++ + ++ A +  SP+L  LH RL+LP              
Sbjct: 65  LPESELAKYKEYYQGLKSTVNEIPESVASK--SPSLRTLHKRLQLPNELTYSTLSRCLTC 122

Query: 77  XXX---GEVN-----------------YDNVSLNIFGKSLLTLAVTSELMKKYPRLPGVV 116
                  ++N                  DN  LNI GK+LL+  VT  +++KYPRLP VV
Sbjct: 123 PSAKLPDKINNPTKGAAFVNTVPTNKYLDNHGLNIMGKNLLSYHVTKSIIQKYPRLPTVV 182

Query: 117 LNAAVDAYINDHVLSNVGRSWGIEPETQSVVDRYAKKENVTITLGKLRYFQNSVDELH-- 174
           LNAAV+AYI++ VL+++ + WGIE ET SV+ RY K E    TLG+L++F NS++     
Sbjct: 183 LNAAVNAYISEAVLAHIAKYWGIEVETTSVLSRYLKMEPFEFTLGRLKFFNNSLNSKDGI 242

Query: 175 ------NFTENNAMAVAVRSIFAAVYSV--DKDFNQTHKFIADHILSRKLDVKDLFAFEQ 226
                 NF+E +A+A++VRSI AA+++V   KD    ++FI DHI+SRKLD+  +F FEQ
Sbjct: 243 ELITGKNFSETSALAMSVRSIIAAIWAVTEQKDSQAVYRFIDDHIMSRKLDITKMFQFEQ 302

Query: 227 PTRELAALCRRQGLERPVSKLLAENGRLSKSPLFIVGVFSGEEKLGEGYGSSLKEAKARA 286
           PTRELA LCRR+GLE+PVSKL+AE+GRLSKSP+FIV VFSGEE LGEGYGSSLKEAKARA
Sbjct: 303 PTRELAMLCRREGLEKPVSKLVAESGRLSKSPVFIVHVFSGEETLGEGYGSSLKEAKARA 362

Query: 287 ATDALLKWYCYEPVHGQQNVVIDQGEVLV 315
           ATDAL+KWYCYEP+  QQ  VID G V+V
Sbjct: 363 ATDALMKWYCYEPL-AQQEPVIDPGTVVV 390


>SPBC3B9.14c [J] KOG3769 Ribonuclease III domain proteins
          Length = 326

 Score =  122 bits (307), Expect = 5e-28
 Identities = 79/280 (28%), Positives = 140/280 (49%), Gaps = 14/280 (5%)

Query: 25  ELSSLKEYKNTISTLLKNP-AHELTSPNLVALHSRLRLPQGXXXXXXXXXXXXXXXGE-V 82
           + + ++ Y N+  +L++N  + ++    +  L  R+ LP+                 +  
Sbjct: 19  DYARVRWYSNSGKSLIENILSSKIAISKVYTLKKRIGLPESYPMKVLVQALIDKTVVQNP 78

Query: 83  NYDNVSLNIFGKSLLTLAVTSELMKKYPRLPGVVLNAAVDAYINDHVLSNVGRSWGIEPE 142
            Y N  L   GK      V   +  KYPRLP   ++   + ++    LS +   WG+E +
Sbjct: 79  QYSNEKLWSIGKVFGDFYVLEYIKCKYPRLPEEAVHGMQNGWMGSKALSQLATIWGLEVQ 138

Query: 143 TQSVVDRYAKKENVTITLGKLRYFQNSVDELHNFTE---NNAMAVAVRSIFAAVYSVDKD 199
                    ++E +  + GK+   +   + L    E   + A   A+ +I   VY + + 
Sbjct: 139 R--------REEGLQESFGKVLAKRADPEMLRKPGEIYKDEAARRAILAILGGVY-LHQG 189

Query: 200 FNQTHKFIADHILSRKLDVKDLFAFEQPTRELAALCRRQGLERPVSKLLAENGRLSKSPL 259
           FN T KFI DHILS++LD + +   + P R+L+ LC+R  L+ PV +++AE GR S+ P+
Sbjct: 190 FNDTKKFINDHILSKQLDPRTMLQLQWPRRQLSRLCKRLSLKEPVYRIIAETGRKSREPV 249

Query: 260 FIVGVFSGEEKLGEGYGSSLKEAKARAATDALLKWYCYEP 299
           F++G +SG   LG+G  SSL  A+ +AA ++L+ +Y + P
Sbjct: 250 FVIGAYSGHHLLGQGQASSLNLAEQQAAYNSLISYYIHSP 289


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.317    0.134    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,045,959
Number of Sequences: 60738
Number of extensions: 664821
Number of successful extensions: 1644
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1638
Number of HSP's gapped (non-prelim): 3
length of query: 315
length of database: 30,389,216
effective HSP length: 106
effective length of query: 209
effective length of database: 23,950,988
effective search space: 5005756492
effective search space used: 5005756492
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)