ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2047 suspect: LH E KOG0172 Amino acid transport and metabolism Lysine-ketoglutarate reductase/saccharopine dehydrogenase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV2047 719938 718823 -372 
         (372 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YIR034c [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine ...   528   e-150
SPAC227.18 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopi...   383   e-106

>YIR034c [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
           dehydrogenase
          Length = 373

 Score =  528 bits (1359), Expect = e-150
 Identities = 255/371 (68%), Positives = 292/371 (77%)

Query: 2   SVTLHLRAETKPLEARXXXXXXXXXXXXXXGFKIFVEESSQSAFDIEEYKKVGATIVPEG 61
           +VTLHLRAETKPLEAR              GFKI+VE+S QS F+I EY++ GA IVP G
Sbjct: 3   AVTLHLRAETKPLEARAALTPTTVKKLIAKGFKIYVEDSPQSTFNINEYRQAGAIIVPAG 62

Query: 62  SWKTAPRDRIIIGLKEMPETDTFPLVHEHIQFAHCYKNQAGWEDVLSRFKRGNGVLYDLE 121
           SWKTAPRDRIIIGLKEMPETDTFPLVHEHIQFAHCYK+QAGW++VL RF +G+G LYDLE
Sbjct: 63  SWKTAPRDRIIIGLKEMPETDTFPLVHEHIQFAHCYKDQAGWQNVLMRFIKGHGTLYDLE 122

Query: 122 FLENDQXXXXXXXXXXXXXXXXXXXIRDWAFKQTHSDSENLGAIEPYENEEAMIKDVRRD 181
           FLENDQ                   +RDWAFKQTHSD E+L A+ PY NE+A++KDV +D
Sbjct: 123 FLENDQGRRVAAFGFYAGFAGAALGVRDWAFKQTHSDDEDLPAVSPYPNEKALVKDVTKD 182

Query: 182 YQLALKRGARKPKVLIIGALGRCGSGAADLLRKCGLPEENIIKWDMKETARGGPFQEIAD 241
           Y+ AL  GARKP VLIIGALGRCGSGA DLL K G+P+ NI+KWD+KET+RGGPF EI  
Sbjct: 183 YKEALATGARKPTVLIIGALGRCGSGAIDLLHKVGIPDANILKWDIKETSRGGPFDEIPQ 242

Query: 242 ADVFINCIYLSKPIPPFINNELLNKPTRRLRTVVDVSADTTNPHNPVPIYTINTTFDAPT 301
           AD+FINCIYLSKPI PF N E LN P RRLRTVVDVSADTTNPHNP+PIYT+ T F+ PT
Sbjct: 243 ADIFINCIYLSKPIAPFTNMEKLNNPNRRLRTVVDVSADTTNPHNPIPIYTVATVFNKPT 302

Query: 302 VQVPTTVGPKLSVCSIDHLPSLLPKEASESFSRDLLPSLEQLPQRATASVWVKARNLYEK 361
           V VPTT GPKLSV SIDHLPSLLP+EASE FS DLLPSLE LPQR TA VWV+A+ L+++
Sbjct: 303 VLVPTTAGPKLSVISIDHLPSLLPREASEFFSHDLLPSLELLPQRKTAPVWVRAKKLFDR 362

Query: 362 FAAKVGRTSKL 372
             A+V R+S+L
Sbjct: 363 HCARVKRSSRL 373


>SPAC227.18 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
           dehydrogenase
          Length = 368

 Score =  383 bits (984), Expect = e-106
 Identities = 189/364 (51%), Positives = 243/364 (65%), Gaps = 3/364 (0%)

Query: 5   LHLRAETKPLEARXXXXXXXXXXXXXXGFKIFVEESSQSAFDIEEYKKVGATIVPEGSWK 64
           L LRAETKPLE R              GF+I +E SSQ AF  +E++++G  +VPEGSW+
Sbjct: 6   LWLRAETKPLEERSALTPRTAKILADAGFQITIERSSQRAFKDKEFERLGFPMVPEGSWR 65

Query: 65  TAPRDRIIIGLKEMPETDTFPLVHEHIQFAHCYKNQAGWEDVLSRFKRGNGVLYDLEFLE 124
            AP+D  IIGLKE+PE D  PL H HIQFAHCYKNQ GW +VLSRF  GNG+LYDLEFL+
Sbjct: 66  HAPKDAYIIGLKELPENDNSPLKHTHIQFAHCYKNQEGWREVLSRFPAGNGLLYDLEFLQ 125

Query: 125 NDQXXXXXXXXXXXXXXXXXXXIRDWAFKQTHSDSENLGAIEPYENEEAMIKDVRRDYQL 184
           +D                       WA +  H + +   AI P+ NE+++++ V R  +L
Sbjct: 126 DDNGRRVAAFGYHAGFAGSAISCLVWAHQLLHPNKQ-FPAIRPFPNEKSLVRHVARQVRL 184

Query: 185 ALKRGARK-PKVLIIGALGRCGSGAADLLRKCGLPEENIIKWDMKETARGGPFQEIADAD 243
           ALK+   + P++L+IGALGRCG+GA DL  K G+P +NI++WD+ ET +GGPF EI ++D
Sbjct: 185 ALKKNNNQYPRILVIGALGRCGTGACDLASKIGIPFDNILRWDINETKKGGPFTEITESD 244

Query: 244 VFINCIYLSKPIPPFINNELLNKPTRRLRTVVDVSADTTNPHNPVPIYTINTTFDAPTVQ 303
           +F+NCIYLS PIP F   E LN P R+LR V DVS DTTNP+NP+PIY +NTTFD PTV+
Sbjct: 245 IFVNCIYLSMPIPKFCTVESLNVPNRKLRVVCDVSCDTTNPNNPIPIYNVNTTFDHPTVE 304

Query: 304 VP-TTVGPKLSVCSIDHLPSLLPKEASESFSRDLLPSLEQLPQRATASVWVKARNLYEKF 362
           V   T  P L V SIDHLP+LLP+E+SE+FS  L+PSL  L     A VWV+A+ LYE  
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLALKDVDNAPVWVRAKKLYETM 364

Query: 363 AAKV 366
             K+
Sbjct: 365 VQKL 368


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,255,752
Number of Sequences: 60738
Number of extensions: 908118
Number of successful extensions: 1951
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1946
Number of HSP's gapped (non-prelim): 2
length of query: 372
length of database: 30,389,216
effective HSP length: 108
effective length of query: 264
effective length of database: 23,829,512
effective search space: 6290991168
effective search space used: 6290991168
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)