ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2074 good A KOG3741 RNA processing and modification Poly(A) ribonuclease subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2074 727056 729023 656
(656 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL025c [A] KOG3741 Poly(A) ribonuclease subunit 458 e-128
7292306 [A] KOG3741 Poly(A) ribonuclease subunit 188 3e-47
CE00424 [A] KOG3741 Poly(A) ribonuclease subunit 183 6e-46
SPBC336.14c [A] KOG3741 Poly(A) ribonuclease subunit 171 3e-42
Hs22053740 [A] KOG3741 Poly(A) ribonuclease subunit 121 4e-27
SPAC1B1.04c [A] KOG3741 Poly(A) ribonuclease subunit 106 1e-22
>YKL025c [A] KOG3741 Poly(A) ribonuclease subunit
Length = 679
Score = 458 bits (1179), Expect = e-128
Identities = 283/697 (40%), Positives = 385/697 (54%), Gaps = 76/697 (10%)
Query: 13 PDSFKGTQCRNIIIHGYCKFENEGCQFNHGNSKETEGQTTDEVP--------TQRSMTPL 64
PD K CRNI I+GYCK E EGC F H S T T ++VP T +MT +
Sbjct: 6 PDWAKDIPCRNITIYGYCKKEKEGCPFKH--SDNTTATTINDVPPPIDVGEATTPTMTSV 63
Query: 65 NVKFNAKTSSSFTP---GKSPAVRSPDFSSLPAFQPG---APVNDQPMLADGPQISGTMS 118
KFNAK S+SFTP G + +S G P I+ ++
Sbjct: 64 P-KFNAKVSASFTPMTVGSDSLTTVTNTTSAATNATGNIAMAATSATASTVNPMINPIVN 122
Query: 119 PSLMNSN-----------ATAFAPSFNPYASESFTPSVSAGGIPSSQELAGTLHGXXXXX 167
SL+N+N TA + +++P+ + FTPS S I ++
Sbjct: 123 SSLVNNNNNNSNISISIPTTASSSNYDPFNAPIFTPS-STSSIHTNANAHSFPFPSIANS 181
Query: 168 XXXXXXXXXXXXAGLPSGGMLGVGHPMQGLXXXXXXGALPVNPIT-QFPTVYPPTHSVLQ 226
+ V PMQ P+ ++P +YPP HS+LQ
Sbjct: 182 GGININATDDNSNNMSMAN--NVPPPMQ---------PPPIESSNLKYPRIYPPPHSLLQ 230
Query: 227 YHLYAPDPPPHLKIPLKPNERTPETLFINNHLRDRLVKNNQTALQVFPRGS--LPDIVGD 284
YHLYAP+ P LK LKPNER+ + LFI N++R+ L K N + LQVFP +P IV D
Sbjct: 231 YHLYAPEQPSSLKSLLKPNERSADQLFIPNNIREDLTKKNLSILQVFPSSGKVIPSIVQD 290
Query: 285 YFGLVPMDFHNRTSDKKRYNGHKNSLYKVFSNLDGKIYFMRRIHDV-KITDSAQVSKPFQ 343
YF LVP++F+N +K +L+KVFSN DGK Y ++R+ ++ K + ++SK +Q
Sbjct: 291 YFNLVPLNFNNNDF------LNKTTLFKVFSNYDGKAYVLKRLPNIDKSMNPNKISKIYQ 344
Query: 344 TWSHLRSANITVLKDSFVTSAFNDSSLCMVFDYYPQSQSLYETYGLANSVNELNQEYLWA 403
WS + N+ +D F T+ F D S+C+VFDYYP S SLY+ + + + YLW
Sbjct: 345 IWSKINCTNLIKFRDIFQTTKFGDLSICLVFDYYPNSLSLYDYHFVNFPKFPITNNYLWI 404
Query: 404 FLVQLTIALQEVHSNGLAL-NDLDWKKVIVTGEPGRIKVTDIGVYDTL-------NYHQE 455
+LVQLT + +HS L++ N L+W+KV++TG+PGRIK++ D L
Sbjct: 405 YLVQLTNVINSIHSQNLSIGNTLNWRKVLITGDPGRIKLSHCNFMDLLFNDDTDTVVSSG 464
Query: 456 GRMLHTEQQQNY---XXXXXXXXXXVQRLCGASGP------------LDDVKSYHIDPLF 500
G + +QQ +Y ++ + P +DD++ ID F
Sbjct: 465 GSTIEGQQQLDYKYLGELLFNLSINIENSNNNTAPKEYRLEEITPQSIDDMR--QIDDKF 522
Query: 501 KKCIQYL-QDTSNNNKNIEDFTKLFSHKVLSVVNSLQYNSEYLEQQLSRELENARLFRLM 559
K ++YL D ++ K+I D T F K+ V+ S Q +EY+E LSRELEN RLFRL+
Sbjct: 523 KDVLKYLISDNGDSKKSIHDLTSHFYDKMFMVLESSQTYTEYMESVLSRELENGRLFRLV 582
Query: 560 CKLNAIYGRLESRIDINWAESGEKFPIILFFDYVFHQKDDTGKNVMDLTHVLRCLNKLDA 619
KLN I+GR+ESRIDINW+ESG KFPIILF+DYVFHQ D GK +MDLTHVLRCLNKLDA
Sbjct: 583 NKLNCIFGRIESRIDINWSESGTKFPIILFYDYVFHQVDSNGKPIMDLTHVLRCLNKLDA 642
Query: 620 GVSERLMLVTPDEMNCIIISYKELKDLIDSTFRALTQ 656
G+ E+LMLVTPDE+NCIIISYKELKDLI+STFR++TQ
Sbjct: 643 GIQEKLMLVTPDELNCIIISYKELKDLIESTFRSITQ 679
>7292306 [A] KOG3741 Poly(A) ribonuclease subunit
Length = 693
Score = 188 bits (477), Expect = 3e-47
Identities = 131/468 (27%), Positives = 233/468 (48%), Gaps = 49/468 (10%)
Query: 228 HLYAPDPPPHLKIPLKPNERTPETLFINNHLRDRLVKNNQTALQVFPRG-----SLPDIV 282
H+Y P P ++ + ++P F+ + +R ++ N+ + + +LP V
Sbjct: 228 HVY-PGPSSNV-VTMQPKTLLESAFFMPDEMRAEVLARNEISNLIMDAAEAAQHALPLEV 285
Query: 283 GDYFGLVPMDFHNRTSDKKRYNGHKNSLYKVFSNLDGKIYFMRRIHDVKITDSAQVSKPF 342
+Y L P++ + K + Y+ N G Y +RRIH ++ S +
Sbjct: 286 ENYHALYPLEPPAQPLHAKLT--FPATTYRATHNTTGYKYCLRRIHGFRL-QSTKCMTLV 342
Query: 343 QTWSHLRSANITVLKDSFVTSAFNDSSLCMVFDYYPQSQSLYETY--------------- 387
+ W L+ N+ L++ F T AF D+SL +V+DY+P SQ+L Y
Sbjct: 343 EMWKKLQHTNVVQLREVFTTKAFGDNSLVLVYDYHPGSQTLLAKYFTPAPETNGYTDPFQ 402
Query: 388 GLANSVNE------------LNQEYLWAFLVQLTIALQEVHSNGLALNDLDWKKVIVTGE 435
G A + L + +W+ ++QLT L+ +H GLA LD K+IVTG+
Sbjct: 403 GEARPFSHKSNMQRTSNGPLLPEATIWSIIMQLTAGLKAIHHAGLACKVLDPTKIIVTGK 462
Query: 436 PGRIK---VTDIGVYDTLNYHQEGRMLHTEQQQNYXXXXXXXXXXVQRLCGASGPLDDVK 492
R ++DI +D N +++ QQ + R C S D+V+
Sbjct: 463 RVRFSSCCISDITQFDP-NASNPLALVNMHQQDDLTALGRLVLALACR-CLQSVQRDNVQ 520
Query: 493 SYHIDPLFKKCIQYLQD------TSNNNKNIEDFTKLFSHKVLSVVNSLQYNSEYLEQQL 546
S ID + + L++ T+NN +++ D + + + +++LQ + E +L
Sbjct: 521 S-SIDMVTRNYSTDLRNFIVYLFTTNNRRSVTDLMPMIGARFYTQLDALQSKIDMQEDEL 579
Query: 547 SRELENARLFRLMCKLNAIYGRLESRIDINWAESGEKFPIILFFDYVFHQKDDTGKNVMD 606
++E+EN RL+R++ KLN+I R + +D W+E+G+++ + LF DY+FH + G+ +D
Sbjct: 580 AKEMENGRLYRILVKLNSINERPDFNLDCTWSETGDRYMLKLFRDYLFHSVTEDGRPWLD 639
Query: 607 LTHVLRCLNKLDAGVSERLMLVTPDEMNCIIISYKELKDLIDSTFRAL 654
H+++CLNKLDAG ER+ L++ DE + +I+SY ELK+ +++ F L
Sbjct: 640 HAHIVQCLNKLDAGSIERVQLMSRDEQSVLIVSYAELKNCLENAFSEL 687
>CE00424 [A] KOG3741 Poly(A) ribonuclease subunit
Length = 676
Score = 183 bits (465), Expect = 6e-46
Identities = 132/451 (29%), Positives = 211/451 (46%), Gaps = 56/451 (12%)
Query: 231 APDPPPHLKIPLKPNERTPETLFINNHLRDRLVKNNQTALQVFPR----GSLPDIVGDYF 286
AP P H+ +T FI+ +R L+ N Q A G +P V +
Sbjct: 214 APLPISHMARFRGKANANLQTQFISPEIRMELI-NRQLAYDTKADSAIIGDIPHSVEHFS 272
Query: 287 GLVPMDFHNRTSDKKRYNGHKNSLYKVFSNLDGKIYFMRRIHDVKITDSAQVSKPFQTWS 346
LVP++ S + YK FS DG Y +RRIH +I + + + W
Sbjct: 273 NLVPLEIAGIQSQ---------TTYKAFSCRDGNYYCLRRIHGNRIQHPGKQTHLVEQWK 323
Query: 347 HLRSANITVLKDSFVTS-AFNDSSLCMVFDYYPQSQSLYETY------------------ 387
L N+ L++ + AF+DSSL +DYYP + +L E +
Sbjct: 324 KLVHGNVVPLREVLINCRAFDDSSLIFAYDYYPLAGTLMEKHFDTKSGTFFDPNNGFRIS 383
Query: 388 -----GLANSVNELNQEYLWAFLVQLTIALQEVHSNGLALNDLDWKKVIVTGEPGRIKVT 442
+ S ++ +W++++Q+ AL+ +HS+GLA LD K+I G +I ++
Sbjct: 384 SPMNVSMPISGTGAHETLIWSYIIQIAAALRAIHSSGLACRTLDLNKIITYGN--KIMIS 441
Query: 443 DIGVYDTLNYHQEGRMLHTEQQQNYXXXXXXXXXXVQRLCGASGPLDDV---------KS 493
G+ D L+ +QQQN A+G D+ +
Sbjct: 442 FCGIQDVLDPDPTT----IQQQQNEDLNMFGNLIVALATGRANGWRKDLYQQLKKFIEDT 497
Query: 494 YHIDPLFKKCIQYLQDTSNNNKNIEDFTKLFSHKVLSVVNSLQYNSEYLEQQLSRELENA 553
Y +D + I +L + S K I + + + +V+ ++Q ++ LE +LSRE+EN
Sbjct: 498 YSMD--LRNVIGFLHNNSTR-KTINEIMPMIGGRFFTVMENMQAKTDVLEAELSREMENG 554
Query: 554 RLFRLMCKLNAIYGRLESRIDINWAESGEKFPIILFFDYVFHQKDDTGKNVMDLTHVLRC 613
RLFRL+ K+N + R+E D W+E+G++F + LF DYVFHQ D GK +D+ H+++C
Sbjct: 555 RLFRLVAKMNTVLERVEHGTDDAWSETGDRFMLKLFRDYVFHQVTDQGKAWLDMAHIVQC 614
Query: 614 LNKLDAGVSERLMLVTPDEMNCIIISYKELK 644
LNKLD G E++ +V+ III Y LK
Sbjct: 615 LNKLDCGSQEKIEMVSRSGDTQIIIDYATLK 645
>SPBC336.14c [A] KOG3741 Poly(A) ribonuclease subunit
Length = 589
Score = 171 bits (433), Expect = 3e-42
Identities = 128/442 (28%), Positives = 216/442 (47%), Gaps = 38/442 (8%)
Query: 227 YHLYAPDPPPHLKIP-LKPNERTPETLFINNHLRDRLVKNNQTALQVFPRGSLPDIVGDY 285
Y+LY P P P +P L P ERT LFI++ R+R+ + ++ + Q +LP I+ Y
Sbjct: 163 YNLYNPQPSP---MPTLLPYERTVNGLFIDDTTRERIERKSEASRQTI--SALPSIISSY 217
Query: 286 FGLVPMDFHNRTSDKKRYNGHKNSLYKVFSNLDGKIYFMRRIHDVKIT-DSAQVSKPFQT 344
L P++ + K G + YK S++DG Y ++R+ D I D++ V K
Sbjct: 218 TSLAPLN--TKLYRYKEQIGFSSWTYKCTSSIDGNAYVLKRLQDCSINIDTSTVDK---- 271
Query: 345 WSHLRSANITVLKDSFVTSAFNDSSLCMVFDYYPQSQSLYETYGLANSVNELNQE----- 399
++ NI +F T F+DSSL +++D+YP + +L E Y L NS N + E
Sbjct: 272 LKNVFHPNIVPFHSAFHTDTFHDSSLLLIYDFYPCTTTLGELY-LNNSKNSVKLEENRKI 330
Query: 400 ---YLWAFLVQLTIALQEVHSNGLALNDLDWKKVIVTGEPGRIKVTDIGVYDTLNYHQEG 456
LW + QLTIAL +H +G A N L +++V + RI+++ Y+ + ++
Sbjct: 331 PERELWNYFFQLTIALSYLHKSGFACNKLTPSRILV-DQTERIRISGCADYELVVSNKP- 388
Query: 457 RMLHTEQQQNYXXXXXXXXXXVQRLCGASGPLDDVKSYHIDPL-------FKKCIQYLQD 509
L ++Q++ A+G D S + F K + Y
Sbjct: 389 -PLEERKKQDFVDLGVVIANL------ATGRTDMDMSSAARAIYSTYSREFYKAVLYFVS 441
Query: 510 TSNNNKNIEDFTKLFSHKVLSVVNSLQYNSEYLEQQLSRELENARLFRLMCKLNAIYGRL 569
+KN+E F + +++S E +E+++S ++ R F ++CK+ I
Sbjct: 442 EVPEDKNLELFLQNHIESFFPIMSSPYVECEKMERKISDAFQHGRFFNILCKIMFIIDNN 501
Query: 570 ESRIDINWAESGEKFPIILFFDYVFHQKDDTGKNVMDLTHVLRCLNKLDAGVSERLMLVT 629
+ + A E I L DY+FHQ D+ V+DL VL L KLDAG+++ + L++
Sbjct: 502 RASREYPIAREKEISLIYLLRDYLFHQIDEDECPVIDLYQVLNRLGKLDAGINQAIALIS 561
Query: 630 PDEMNCIIISYKELKDLIDSTF 651
DE++C+ +SY ELK +D+ +
Sbjct: 562 RDELDCVSVSYGELKAWLDNVY 583
>Hs22053740 [A] KOG3741 Poly(A) ribonuclease subunit
Length = 174
Score = 121 bits (303), Expect = 4e-27
Identities = 54/154 (35%), Positives = 99/154 (64%)
Query: 501 KKCIQYLQDTSNNNKNIEDFTKLFSHKVLSVVNSLQYNSEYLEQQLSRELENARLFRLMC 560
K I YL N +++ D + + + +++ Q ++ +E+ L++E++N RLFRL+
Sbjct: 13 KNLILYLLTDQNRMRSVNDIMPMIGARFYTQLDAAQMRNDVIEEDLAKEVQNGRLFRLLA 72
Query: 561 KLNAIYGRLESRIDINWAESGEKFPIILFFDYVFHQKDDTGKNVMDLTHVLRCLNKLDAG 620
KL I R E + D W+E+G+++ + LF D++FHQ + G +DL+H++ CLNKLDAG
Sbjct: 73 KLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWIDLSHIISCLNKLDAG 132
Query: 621 VSERLMLVTPDEMNCIIISYKELKDLIDSTFRAL 654
V E++ L++ DE + ++++Y +LK ++TF+ L
Sbjct: 133 VPEKISLISRDEKSVLVVTYSDLKRCFENTFQEL 166
>SPAC1B1.04c [A] KOG3741 Poly(A) ribonuclease subunit
Length = 604
Score = 106 bits (265), Expect = 1e-22
Identities = 104/421 (24%), Positives = 175/421 (40%), Gaps = 63/421 (14%)
Query: 278 LPDIVGDYFGLVPMDFHNRTSDKKRYNGHKNSLYKVFSNLDGKIYFMRRIHDVKITDSAQ 337
LP V Y L+P RY S+YKV +G Y ++ D + +
Sbjct: 188 LPSHVLSYHSLIPRRMIVTVLPVLRY---ATSIYKVIDGNNGLPYSFVQLRDFTLLNDRN 244
Query: 338 VSKPFQTWSHLRSANITVLKDSFVTSAFNDS-------------------SLCMVFDYYP 378
++ W+ + S ++ ++++F T AF ++ V++Y P
Sbjct: 245 ITN-VSPWTKVDSPHVIKIREAFTTHAFEQKCMLSFIYFENVALYTNLLLAIVFVYNYLP 303
Query: 379 QSQSLYETYGLA------------NSVNELNQEYLWAFLVQLTIALQEVHSNGLALNDLD 426
SLY+ + + + + +E LW F QL AL +HS+GLA +
Sbjct: 304 SCPSLYDLFFASPVFRKRTSSFYFSQPLKATKEVLWCFASQLISALYSIHSSGLAAKMVS 363
Query: 427 WKKVIVTGEPGRIKVTDIGVYDTLNYHQEGRMLHTEQQQN------YXXXXXXXXXXVQR 480
K V++ G+ R+ + +G+ D + +E T Q+N
Sbjct: 364 LKNVLMVGKM-RLAIFGLGIMDVIQ--EESTEPLTSLQRNDCRDVGLILLALATDTENVT 420
Query: 481 LCGASGPLDDVKSY-HIDPLFKKCIQYLQDTSNNNKNIEDFTKLFSHKVLSVVNSLQYNS 539
L A L +K+ D + I+ L N I+ +++ S+
Sbjct: 421 LSTAKAHLTRLKTIVSTDASLVELIEVL--IFNEELRIQTLLPTMLSYMVNNYESVLLME 478
Query: 540 EYLEQQLSRELENARLFRLMCKLNAIYGRLESRIDINWAESGEKFPIILFFDYVFHQK-- 597
+ E L+ ++EN RL RL+ KL + R E D +W+ SG F I LF Y+F +
Sbjct: 479 DVYETYLAEQVENDRLLRLLLKLEFLDDRPEYVDDPDWSASGVYFVIRLFRKYMFQVQTI 538
Query: 598 DDTGK------------NVMDLTHVLRCLNKLDAGVSERLMLVTPDEMNCIIISYKELKD 645
DD K +++ H+L CLNKLDAG E+++L DE II+S+KE++
Sbjct: 539 DDASKKPTLQSTTTPPRKLLNKAHLLSCLNKLDAGTDEQILL--EDEFTRIIMSFKEVRT 596
Query: 646 L 646
+
Sbjct: 597 I 597
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,911,772
Number of Sequences: 60738
Number of extensions: 1847756
Number of successful extensions: 5351
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 5321
Number of HSP's gapped (non-prelim): 15
length of query: 656
length of database: 30,389,216
effective HSP length: 113
effective length of query: 543
effective length of database: 23,525,822
effective search space: 12774521346
effective search space used: 12774521346
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)