ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2120 good A KOG2793 RNA processing and modification Putative N2,N2-dimethylguanosine tRNA methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2120 746966 746169 -266
(266 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL024c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 275 4e-74
SPAC23C4.06c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA me... 73 5e-13
At5g49560 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methy... 67 3e-11
At5g44170 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methy... 64 2e-10
At3g50850 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methy... 64 2e-10
Hs17475528 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA meth... 63 4e-10
YBR271w [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 59 9e-09
CE26910 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methylt... 57 3e-08
At1g73320 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methy... 54 2e-07
YJR129c [R] KOG2497 Predicted methyltransferase 50 3e-06
>YNL024c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 246
Score = 275 bits (704), Expect = 4e-74
Identities = 147/240 (61%), Positives = 177/240 (73%), Gaps = 9/240 (3%)
Query: 34 FEELVPARPIEHLGKIDMSFNGKLPSPLIINEDGGESGCGGKVWIAGELLCEYILEKSDK 93
F +LV RP EHLG+ D+SF GKL L I EDGGESGCGGKVWIAGELLCEYILEKS
Sbjct: 8 FGDLVVPRPKEHLGQTDLSFGGKLLPALKICEDGGESGCGGKVWIAGELLCEYILEKS-V 66
Query: 94 EHLLSHLFPDGN-CNSVLELGSGTGLVGLCVGLMDQANEYSDREVYISDIDQLLGLMESN 152
+HLLS VLELGSGTGLVGLCVGL+++ + +VY++DID+L+ L++ N
Sbjct: 67 DHLLSKTVNGTKQFKKVLELGSGTGLVGLCVGLLEKNTFHDGTKVYVTDIDKLIPLLKRN 126
Query: 153 IQVNGLDDKVHAEVLWWGNPLPDVFVKKP-------VDLVLAADCVYLEAAFPLLEKTLL 205
I+++ + +V A LWWG PL F + VDLVLAADCVYLE AFPLLEKTLL
Sbjct: 127 IELDEVQYEVLARELWWGEPLSADFSPQEGAMQANNVDLVLAADCVYLEEAFPLLEKTLL 186
Query: 206 ELTDGENVPIILMAYKKRRKADKHFFQKIKKNFKIVEIRDFINFDKYLKQRTHLFQLMRE 265
+LT N P+ILMAYKKRRKADKHFF KIK+NF ++EI DF F+ YLK+RTHLFQL+R+
Sbjct: 187 DLTHCINPPVILMAYKKRRKADKHFFNKIKRNFDVLEITDFSKFEHYLKERTHLFQLIRK 246
>SPAC23C4.06c [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 327
Score = 72.8 bits (177), Expect = 5e-13
Identities = 51/161 (31%), Positives = 88/161 (53%), Gaps = 15/161 (9%)
Query: 76 VWIAGELLCEYILEKSDKEHLLSHLFPDGNCNSVLELGSGTGLVGLCVGLMDQANEYSDR 135
+W AG + + IL SD H + F + +VLELGSG G+VG+ + A++Y
Sbjct: 158 LWDAGVVFSKKIL--SDDWH---YSFSNRKDINVLELGSGCGIVGISI-----ASKYPRA 207
Query: 136 EVYISDIDQLLGLMESNIQVN--GLDDKVHAEVLWWGNPLPDVFVKKPVDLVLAADCVYL 193
V ++D + + ME N++ N + + + +++L WG+ +P F ++ D ++ +D +Y
Sbjct: 208 LVSMTDTEDAIEFMEKNVEKNKSAMSNNITSDILVWGHDIPRKF-RRHWDYIVMSDVMYN 266
Query: 194 EAAFPLLEKTLLELTDGENVPIILMAYKKRRKADKHFFQKI 234
E++F LE +L EL D + +AYKKR +K F I
Sbjct: 267 ESSFSDLEASLQELMDKNTK--LYIAYKKRHDNEKTFMSNI 305
>At5g49560 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 274
Score = 66.6 bits (161), Expect = 3e-11
Identities = 47/139 (33%), Positives = 74/139 (52%), Gaps = 13/139 (9%)
Query: 108 SVLELGSGTGLVGLCVGLMDQANEYSDREVYISDIDQLLGLMESNIQVNG-----LDDKV 162
++LELGSGTGLVG+ + AN V ++D+ +L + N + N KV
Sbjct: 108 NILELGSGTGLVGIAAAITLSAN------VTVTDLPHVLDNLNFNAEANAEIVERFGGKV 161
Query: 163 HAEVLWWGNPLPDVFVKKPVDLVLAADCVYLEAAF-PLLEKTLLELTDGENVPIILMAYK 221
+ L WG + + VDL+LA+D VY + + PLL+ L +G+ + I LMA+
Sbjct: 162 NVAPLRWGEADDVEVLGQNVDLILASDVVYHDHLYEPLLKTLRLMQLEGKRL-IFLMAHL 220
Query: 222 KRRKADKHFFQKIKKNFKI 240
+R K + FF+K +K F +
Sbjct: 221 RRWKKESVFFKKARKLFDV 239
>At5g44170 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 234
Score = 63.9 bits (154), Expect = 2e-10
Identities = 58/227 (25%), Positives = 104/227 (45%), Gaps = 20/227 (8%)
Query: 49 IDMSFNGKLPSPLIINEDGGESGCGGKVWIAGELLCEYILEKSDKEHLLSHLFP------ 102
ID++ NG + L I +D G G VW +L ++ S + S P
Sbjct: 9 IDLTVNG---TKLSIQQDNGSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAEL 65
Query: 103 -DGNCNSVLELGSGTGLVGLCVGLMDQANEYSDREVYISDIDQLLGLMESNIQVN--GLD 159
D +ELG+G G+ G+ L+ E+ ++DI ++ ++ N++ N L
Sbjct: 66 FDFRRRRGIELGTGCGVAGMAFYLLGLT------EIVLTDIAPVMPALKHNLKRNKTALG 119
Query: 160 DKVHAEVLWWGNPLPDVFVKKPVDLVLAADCVYLEAAFPLLEKTLLELTDGENVPIILMA 219
+ +++W N +K P DLV+AAD VY+E + L T +EL ++ ++L
Sbjct: 120 KSLKTSIVYWNNRDQISALKPPFDLVIAADVVYIEESVGQL-VTAMELLVADDGAVLLGY 178
Query: 220 YKKRRKADKHFFQKIKKNFKIVEIRDFINFDKYLKQRTHLFQLMREK 266
+ +ADK F++ FKI ++ Y + T ++ + R+K
Sbjct: 179 QIRSPEADKLFWELCDIVFKIEKVPHEHLHSDYAYEETDVY-IFRKK 224
>At3g50850 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 251
Score = 63.9 bits (154), Expect = 2e-10
Identities = 44/139 (31%), Positives = 71/139 (50%), Gaps = 12/139 (8%)
Query: 108 SVLELGSGTGLVGLCVGLMDQANEYSDREVYISDIDQLLGLMESNIQVNG-----LDDKV 162
+++ELGSGTG+VG+ AN V ++D+ ++ ++ N N KV
Sbjct: 91 NIVELGSGTGIVGIAAAATLGAN------VTVTDLPNVIENLKFNADANAQVVAKFGGKV 144
Query: 163 HAEVLWWGNPLPDVFVKKPVDLVLAADCVYLEAAF-PLLEKTLLELTDGENVPIILMAYK 221
H L WG + + VDL+LA+D VY + PLL+ L +G + + LMA+
Sbjct: 145 HVASLRWGEIDDVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHL 204
Query: 222 KRRKADKHFFQKIKKNFKI 240
KR K + FF+K ++ F +
Sbjct: 205 KRWKKESIFFKKARRFFDV 223
>Hs17475528 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 257
Score = 63.2 bits (152), Expect = 4e-10
Identities = 56/180 (31%), Positives = 84/180 (46%), Gaps = 24/180 (13%)
Query: 73 GGKVWIAGELLCEYILEKSDKEHLLSHLFPDGNCNSVLELGSGTGLVGLCVGLMDQANEY 132
G VW + +LC Y LE + K++ + +V+E+G+GTGLV + L+
Sbjct: 78 GAVVWPSALVLC-YFLETNAKQYNMVD-------KNVIEIGAGTGLVSIVASLL------ 123
Query: 133 SDREVYISDIDQLLGLMESNIQVNGLDDKVH---AEVLWWGNPLPDVFVKKP--VDLVLA 187
V +D+ +LLG ++ NI N H + L WG L F + D +LA
Sbjct: 124 -GAHVTATDLPELLGNLQYNISRNTKMKSKHLPQVKELSWGVALDTNFPRSSNNFDYILA 182
Query: 188 ADCVYLEAAFPLLEKTLLELTD-GENVPIILMAYKKRRKADKHFFQKIKKNFKIVEIRDF 246
AD VY A P LE+ L+ + IIL A K R + + F + K+ F + EI F
Sbjct: 183 ADVVY---AHPFLEELLITFDHLCKETTIILWAMKFRLEKENKFVDRFKELFDLEEISSF 239
>YBR271w [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 419
Score = 58.5 bits (140), Expect = 9e-09
Identities = 37/127 (29%), Positives = 69/127 (54%), Gaps = 7/127 (5%)
Query: 73 GGKVWIAGELLCEYILEKSDKEHLLS-HLFPDGNCNS--VLELGSGTGLVGLCVGLM-DQ 128
G K W + +L + +++ D H + ++ + + VLELG+GTGLVGL L +
Sbjct: 218 GWKTWGSSLILSQLVVDHLDYLHTTNVNMLANSDIKQIKVLELGAGTGLVGLSWALKWKE 277
Query: 129 ANEYSDREVYISDIDQLLGLMESNIQVNGLDDKVHAEVLWWGNP---LPDVFVKKPVDLV 185
+ E++++D+ +++ ++ N+ +N L D V AE+L W NP + + D++
Sbjct: 278 LYGTENIEIFVTDLPEIVTNLKKNVSLNNLGDFVQAEILDWTNPHDFIDKFGHENEFDVI 337
Query: 186 LAADCVY 192
L AD +Y
Sbjct: 338 LIADPIY 344
>CE26910 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 282
Score = 57.0 bits (136), Expect = 3e-08
Identities = 42/137 (30%), Positives = 72/137 (51%), Gaps = 18/137 (13%)
Query: 109 VLELGSGTGLVGLCVGLMDQANEYSDREVYISDIDQLLGLMESNIQVNG--LDDKVHAEV 166
VLELGSGTG+ G+ + + +V I+D+ + L L+E N++ N +++ +V
Sbjct: 133 VLELGSGTGVGGIALAAL-------GADVIITDLPERLALIEKNVEANRKLTGNRIKVQV 185
Query: 167 LWWGNPLPDVFVKKPVDLVLAADCVYLEAAF-PLLEKTLLELTDGENVPIIL--MAYKKR 223
L W + + +D+VLA DCVY + PL+ TLL D + + ++ +
Sbjct: 186 LDWTKDR----IPEGLDMVLAIDCVYYNSTIDPLI--TLLNDCDAKEIMVVSEERDIGEA 239
Query: 224 RKADKHFFQKIKKNFKI 240
A K FF+ I+K F++
Sbjct: 240 HLAQKSFFKDIQKFFRL 256
>At1g73320 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
Length = 314
Score = 54.3 bits (129), Expect = 2e-07
Identities = 55/214 (25%), Positives = 90/214 (41%), Gaps = 25/214 (11%)
Query: 57 LPSPLIINEDGGESGCGGKVWIAGELLCEYILEKSDKEHLLSHLFPDGNCNSVLELGSGT 116
L SP +N G G +W +G +L +++ EH + ++ELGSG
Sbjct: 106 LQSPCSLNTPGVT---GSVMWDSGVVLGKFL------EHSVDSKVLSLEGKKIVELGSGC 156
Query: 117 GLVGLCVGLMDQANEYSDREVYISDIDQLLGLMESNIQV-----NGLDDKVHAEVLWWGN 171
GLVG C+ + N ++D+ L L++ NIQ N + E++W +
Sbjct: 157 GLVG-CIAALLGGN------AVLTDLPDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDD 209
Query: 172 PLPDVFVKKPVDLVLAADCVYLEAAFPLLEKTLLELTDGENVPIILMAYKKRRKADKHFF 231
P PD+ P +D +Y E A L KTLL+L + I L + ++F
Sbjct: 210 PDPDLIEPFP---DYGSDVIYSEEAVHHLVKTLLQLC-SDQTTIFLSGELRNDAVLEYFL 265
Query: 232 QKIKKNFKIVEIRDFINFDKYLKQRTHLFQLMRE 265
+ K+F I + Y R L+ L ++
Sbjct: 266 ETALKDFAIGRVEQTQWHPDYRSHRVVLYVLEKK 299
>YJR129c [R] KOG2497 Predicted methyltransferase
Length = 339
Score = 50.1 bits (118), Expect = 3e-06
Identities = 44/171 (25%), Positives = 81/171 (46%), Gaps = 14/171 (8%)
Query: 70 SGCGGKVWIAGELLCEYILEKSDKEHLLSHLFPDGNCN-SVLELGSGTGLVGLCVGLMDQ 128
S G + W A + ++++ K +E DG +VLE+G+GTG+V L + + +
Sbjct: 130 STTGFRTWEAALYMGDFLIHKPLQELAPVQGQDDGKKKLNVLEVGAGTGIVSLVI--LQK 187
Query: 129 ANEYSDREVYISDID------QLLGLMESNIQVNGLDDKVHAEVLWWGNPLPDVFVKKPV 182
+E+ ++ +Y++D D QL E N +V + + + LWWG+ V + +
Sbjct: 188 YHEFVNK-MYVTDGDSNLVETQLKRNFELNNEVRENEPDIKLQRLWWGSDR----VPEDI 242
Query: 183 DLVLAADCVYLEAAFPLLEKTLLELTDGENVPIILMAYKKRRKADKHFFQK 233
DLV+ AD Y P L + L E + + L++ R ++ F +
Sbjct: 243 DLVVGADVTYDPTILPDLCECLAECLALDRCKLCLLSATIRSESTVQLFSQ 293
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.142 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,342,815
Number of Sequences: 60738
Number of extensions: 825475
Number of successful extensions: 1749
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1737
Number of HSP's gapped (non-prelim): 10
length of query: 266
length of database: 30,389,216
effective HSP length: 104
effective length of query: 162
effective length of database: 24,072,464
effective search space: 3899739168
effective search space used: 3899739168
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)