ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2195 good S KOG0396 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2195 766404 765001 -468
(468 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL097w [S] KOG0396 Uncharacterized conserved protein 339 6e-93
SPBC106.13 [S] KOG0396 Uncharacterized conserved protein 112 1e-24
At3g55070 [S] KOG0396 Uncharacterized conserved protein 78 2e-14
7301214_1 [S] KOG0396 Uncharacterized conserved protein 70 9e-12
>YIL097w [S] KOG0396 Uncharacterized conserved protein
Length = 516
Score = 339 bits (869), Expect = 6e-93
Identities = 194/509 (38%), Positives = 286/509 (56%), Gaps = 45/509 (8%)
Query: 5 NEPNVDFHLKLNQQQFNIPSELLKRNWDKFNNLYTSYSVELQGQFATLNTLLE--DPNKD 62
NEP+VDFHLKLNQQ F+IP ELL + + + L A LN + E D D
Sbjct: 8 NEPDVDFHLKLNQQLFHIPYELLSKRIKHTQAVINKETKSLHEHTAALNQIFEHNDVEHD 67
Query: 63 EQSIEKLNDIINTVNILQKRLSQLHDNELTILERIEKRVEYFKQFERFK-------YDSN 115
E ++ K+ ++I V+ +++ L+ + IL RI+KR+E+F + + K ++ N
Sbjct: 68 ELALAKITEMIRKVDHIERFLNTQIKSYCQILNRIKKRLEFFHELKDIKSQNSGTSHNGN 127
Query: 116 TD----KLLKWYRSYTDLLIADYLVRHGSNSIDHKNKIASNSGIEFIKTRGLEDLIDYEV 171
+ KL++WY+SYT++LI DYL R+ + + K NSG+ F+K L+DLIDY+V
Sbjct: 128 NEGTRTKLIQWYQSYTNILIGDYLTRNNPIKYNSETKDHWNSGVVFLKQSQLDDLIDYDV 187
Query: 172 LIEANQISMELIENKNLRPLLEWIENNSAHLTEKGSHLQFQALLQEYIELVRCSDYKAAI 231
L+EAN+IS L+ +NL PL+ WI N LT+K S L+FQA LQEYIEL++ +Y AI
Sbjct: 188 LLEANRISTSLLHERNLLPLISWINENKKTLTKKSSILEFQARLQEYIELLKVDNYTDAI 247
Query: 232 RCFQTHLAHFANIYPKELKLAAGILAFFKSC--------LNNGRDDEVTNEQ-------K 276
CFQ L F +LKLA+G+L F K C ++G D E Q +
Sbjct: 248 VCFQRFLLPFVKSNFTDLKLASGLLIFIKYCNDQKPTSSTSSGFDTEEIKSQSLPMKKDR 307
Query: 277 LFHAYFRKQMYRPHPLSSISSSNV-------VHNAELCRYGPLLDHERWESLNKMFLHEF 329
+F +F K + R ++++++ + + + RY LLD +RW LN +FL +F
Sbjct: 308 IFQHFFHKSLPRITSKPAVNTTDYDKSSLINLQSGDFERYLNLLDDQRWSVLNDLFLSDF 367
Query: 330 YSLYKISYHDPXXXXXXXXXXXXKTKDCGH----------TISAQLIPHENPEVNEYIKS 379
YS+Y IS +DP KT+DC H T +A E ++ +
Sbjct: 368 YSMYGISQNDPLLIYLSLGISSLKTRDCLHPSDDENGNQETETATTAEKEVEDLQLFTLH 427
Query: 380 NVVNTDCPVCNHDIFPLSENLPFAHHIQSSLFENPVMLPNGNIYDSEKLISLSRKLNKMG 439
++ +CPVC+ P+++ LPFAHHIQS LFENP++LPNGN+YDS+KL L++ L K
Sbjct: 428 SLKRKNCPVCSETFKPITQALPFAHHIQSQLFENPILLPNGNVYDSKKLKKLAKTLKKQN 487
Query: 440 YTKLKENQVMDPIDKSIYATTDFIKMYPT 468
L Q+MDP+D I+ +D IKMYPT
Sbjct: 488 LISLNPGQIMDPVDMKIFCESDSIKMYPT 516
>SPBC106.13 [S] KOG0396 Uncharacterized conserved protein
Length = 404
Score = 112 bits (280), Expect = 1e-24
Identities = 96/393 (24%), Positives = 166/393 (41%), Gaps = 75/393 (19%)
Query: 50 ATLNTLLEDPNKD-EQSIEKLNDIINTVNILQKRLSQLHDNELTILERIEKRVEYFKQFE 108
+TL TL+ N ++ IEK++ +++ V+ ++K++ LHD E +++ + R+ + + +
Sbjct: 43 STLTTLISQENASMDEKIEKIDSLLSRVSTVKKKMKHLHDCEALFIKQTKSRLLFMNRLQ 102
Query: 109 RFKYDSNTDKLLKWYRSYTDLLIADYLVRHGSNSIDHKNKIASNSGIEFIKTRGLEDLID 168
+ D + L W R + L+ADY++ +G + + K LE+L+D
Sbjct: 103 GIR-DMESADFLDWSRVRLNRLVADYMMANGYHG----------AAALLCKDSQLENLVD 151
Query: 169 YEVLIEANQISMELIENKNLRPLLEWIENNSAHLTEKGSHLQFQALLQEYIELVRCSDYK 228
+ + Q+ + I + L+ +L W + A L + S L+ + LQ +IEL++
Sbjct: 152 LGIY-KRYQLIHDSILQQELKEVLSWCSEHRAILKKNNSTLELEVRLQRFIELIKSKKLC 210
Query: 229 AAIRCFQTHLAHFANIYPKELKLAAGILAFFKSCLNNGRDDEVTNEQKLFHAYFRKQMYR 288
AI + H +AN +P L+LAA +LAF E TN
Sbjct: 211 QAIAFAKAHFGTWANEHPARLQLAAALLAF----------PEFTNGSP------------ 248
Query: 289 PHPLSSISSSNVVHNAELCRYGPLLDHERWESLNKMFLHEFYSLYKISYHDPXXXXXXXX 348
Y LL +RWE L +F F +++ I
Sbjct: 249 --------------------YSLLLSDDRWEYLASLFTSNFTAVHNIPSVPLLHALLAAG 288
Query: 349 XXXXKTKDCGHTISAQLIPHENPEVNEYIKSNVVNTDCPVCNHDIFPLSENLPFAHHIQS 408
KT C + +NP ++S V CPVC + L + LP+AH QS
Sbjct: 289 LSSLKTPLCYLDAN-----DDNPLA---LQSQTVK-QCPVCTPCLNDLGKALPYAHITQS 339
Query: 409 SLFEN-----------PVMLPNGNIYDSEKLIS 430
++ ++ PV PNG +Y + LIS
Sbjct: 340 AIVDSLTGEGLDSDNCPVAFPNGRVYGIQSLIS 372
>At3g55070 [S] KOG0396 Uncharacterized conserved protein
Length = 418
Score = 78.2 bits (191), Expect = 2e-14
Identities = 89/436 (20%), Positives = 164/436 (37%), Gaps = 97/436 (22%)
Query: 13 LKLNQQQFNIPSELLKRNWDKFNNLYTSYSVELQGQFATLNTLLEDPNKDEQSIEKLNDI 72
LKL Q +P E K+ + S+ E+ + L + + ++ +L +
Sbjct: 41 LKLEHQLLRVPFEHYKKT---IRTNHRSFEKEVSTIVNGVGELADSDWSKDDTVSRLTCL 97
Query: 73 INTVNILQKRLSQLHDNELTILERIEKRVEYFKQFERFKYDSNTDKLLKWYRSYTDLLIA 132
+ + L+++L + + E +R R+++ + + + +W + ++
Sbjct: 98 VTRLQGLKRKLEEGSNVENLQAQRCRARIDHLDSVD-------VENITEWNNTKLKRILV 150
Query: 133 DYLVRHGSNSIDHKNKIASNSGIEFIKTRGLEDLIDYEVLIEANQISMELIENKNLRPLL 192
DY++R + + K++ +S I DL+D ++ EA ++ ++ ++N+ + L
Sbjct: 151 DYMLR--MSYFETATKLSESSNIM--------DLVDIDIFREAKKV-IDALKNREVASAL 199
Query: 193 EWIENNSAHLTEKGSHLQFQALLQEYIELVR---CSDYKAAIRCFQTHLAHFANIYPKEL 249
W +N L + S +FQ LQE+IELVR YK AI+ + HLA + + KEL
Sbjct: 200 TWCADNKTRLKKSKSKFEFQLRLQEFIELVRVDTAESYKKAIQYARKHLASWGTTHMKEL 259
Query: 250 KLAAGILAFFKSCLNNGRDDEVTNEQKLFHAYFRKQMYRPHPLSSISSSNVVHNAELCRY 309
+ LA FKS E +Y
Sbjct: 260 QHVLATLA-FKS-----------------------------------------TTECSKY 277
Query: 310 GPLLDHERWESLNKMFLHEFYSLYKISYHDPXXXXXXXXXXXXKTKDCGHTISAQLIPHE 369
L + +W+ L F EF LY ++ KT
Sbjct: 278 KVLFELRQWDVLVDQFKQEFCKLYGMTMEPLLNIYLQAGLSALKT--------------- 322
Query: 370 NPEVNEYIKSNVVNTDCPVCNHDIFPLSENLPFA--HH------IQSSLFEN---PVMLP 418
Y + P+ + L+ LPF+ HH I L + P +LP
Sbjct: 323 -----PYGLEEGCTKEDPLSQENFRKLALPLPFSKQHHSKLVCYISKELMDTENPPQVLP 377
Query: 419 NGNIYDSEKLISLSRK 434
NG +Y ++ L ++ K
Sbjct: 378 NGYVYSTKALKEMAEK 393
>7301214_1 [S] KOG0396 Uncharacterized conserved protein
Length = 405
Score = 69.7 bits (169), Expect = 9e-12
Identities = 71/379 (18%), Positives = 148/379 (38%), Gaps = 86/379 (22%)
Query: 69 LNDIINTVNILQKRLSQLHDNELTILERIEKRVEYFKQF---ERFKYDSNTDKLLKWYRS 125
++++ + +L+++ + ++EL++ + ++++++ K D + +W R
Sbjct: 63 VDNVAQKLQVLKRKAEESINDELSVTQICKRKLDHLKGITPPSSVTGDLWQGSVDQWKRI 122
Query: 126 YTDLLIADYLVRHGSNSIDHKNKIASNSGIEFIKTRGLEDLIDYEVLIEANQISMELIEN 185
D L+ ++L+R G + ++A+ S + L + ++ + ++ +L +
Sbjct: 123 RLDRLVIEHLLRMGY--YETAEELAAKSDVRH--------LTNLDIFQTSREVEDDLASH 172
Query: 186 KNLRPLLEWIENNSAHLTEKGSHLQFQALLQEYIELVRCSDYKAAIRCFQTHLAHFANIY 245
+ +L W +N + L + S ++F +QE+IELVR
Sbjct: 173 STTKCVL-WCIDNKSKLRKINSTIEFSLRVQEFIELVR---------------------- 209
Query: 246 PKELKLAAGILAFFKSCLNNGRDDEVTNEQKLFHAYFRKQMYRPHPLSSISSSNVVHNAE 305
N R + V + ++ F AY + Q+ + ++ + AE
Sbjct: 210 ------------------QNQRFEAVKHSRRYFPAYEKTQLNEICHVMALLAYPADAQAE 251
Query: 306 LCRYGPLLDHERWESLNKMFLHEFYSLYKISYHDPXXXXXXXXXXXXKTKDCGHTISAQL 365
Y +D +RW+ L F E Y L+++S KT C
Sbjct: 252 --HYRKYMDPQRWQKLVLDFRQENYRLFQLSSTSVFSAAVQAGLSALKTPHC-------- 301
Query: 366 IPHENPEVNEYIKSNVVNTDCPVCNHDIFPLSENLPFAHHIQSSLF-----------ENP 414
N +CPVC D+ ++ LP++H +QS L P
Sbjct: 302 -----------YAQTCRNLNCPVCQEDLNRIALKLPYSHCVQSRLICRVTGLPLNEHNQP 350
Query: 415 VMLPNGNIYDSEKLISLSR 433
+MLPNG I+ L +++
Sbjct: 351 MMLPNGQIFGQMALPDITK 369
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,345,927
Number of Sequences: 60738
Number of extensions: 1212403
Number of successful extensions: 3813
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3798
Number of HSP's gapped (non-prelim): 7
length of query: 468
length of database: 30,389,216
effective HSP length: 110
effective length of query: 358
effective length of database: 23,708,036
effective search space: 8487476888
effective search space used: 8487476888
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)