ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2252 good R KOG2728 General function prediction only Uncharacterized conserved protein with similarity to phosphopantothenoylcysteine synthetase/decarboxylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2252 781831 780779 -351
(351 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL083c [R] KOG2728 Uncharacterized conserved protein with simil... 490 e-138
SPCC4B3.18 [R] KOG2728 Uncharacterized conserved protein with si... 235 6e-62
7300482 [R] KOG2728 Uncharacterized conserved protein with simil... 198 1e-50
CE22938 [R] KOG2728 Uncharacterized conserved protein with simil... 179 5e-45
At5g02080 [R] KOG2728 Uncharacterized conserved protein with sim... 176 5e-44
Hs13375919 [R] KOG2728 Uncharacterized conserved protein with si... 170 3e-42
>YIL083c [R] KOG2728 Uncharacterized conserved protein with similarity to
phosphopantothenoylcysteine synthetase/decarboxylase
Length = 365
Score = 490 bits (1262), Expect = e-138
Identities = 235/345 (68%), Positives = 288/345 (83%), Gaps = 5/345 (1%)
Query: 5 RIHTSITEVSKAIDRTVDETALPIAHLSDEDNYFRTNPRPVYMDDLLTEATQFIEYQKSQ 64
+IHTS+TE+S AIDRT+ E P+A+ ++E+ YF+TNP+P Y+D+L+ +A +FI+ Q S
Sbjct: 11 QIHTSVTEISHAIDRTIKEELFPVAYTTEEEQYFKTNPKPAYIDELIKDAKEFIDLQYSL 70
Query: 65 GREKIVLVTSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLFNGYSVIFLHREFSLTPF 124
R KIVL+TSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFL NGYSVIFLHREFSLTP+
Sbjct: 71 KRNKIVLITSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLANGYSVIFLHREFSLTPY 130
Query: 125 NRPFIHNTETCFLDYFDEDGSISPKYKDLILQNKRDYDQFIYKEKKLLMLPFTTVNQYLW 184
NR F H+ T FLDY D +G I P++ + +L+NK+ YD+++ KE+KLL+LPFTTVNQYLW
Sbjct: 131 NRSFSHSINTLFLDYIDSEGKIKPEFAENVLKNKKLYDKYMEKEEKLLLLPFTTVNQYLW 190
Query: 185 SLKTIAALMNNSGCLFYLAAAVSDFFVPYSRLPQHKIQSQEANQSLNDGNQSS----TTV 240
SLK+IA L+NNSGCLFYLAAAVSDFFVPYSRLPQHKIQS + N + N + TT
Sbjct: 191 SLKSIAKLLNNSGCLFYLAAAVSDFFVPYSRLPQHKIQSGD-NGKMGANNDTEGTTRTTP 249
Query: 241 GGKLIVNLDPVPKFLRRLVESWATQAMIISFKLETDNNLLIHKAHQALDRYNHQLVIGNL 300
GKLIVNLDPVPKFLRRLVESWATQAMI+SFKLETD ++L++K QALDRYNHQLVIGNL
Sbjct: 250 DGKLIVNLDPVPKFLRRLVESWATQAMIVSFKLETDESMLLYKCTQALDRYNHQLVIGNL 309
Query: 301 LQTRNKEVVFVSEQNRDGDWIRITDDHKNIEKLIVQEIVKRHDAW 345
LQTRNK+V+FVS +NR GDW+R+ + H IE++I+ E++ RHD W
Sbjct: 310 LQTRNKQVIFVSPENRKGDWVRLDEKHHIIEEMIIPEVIARHDKW 354
>SPCC4B3.18 [R] KOG2728 Uncharacterized conserved protein with similarity to
phosphopantothenoylcysteine synthetase/decarboxylase
Length = 316
Score = 235 bits (600), Expect = 6e-62
Identities = 140/325 (43%), Positives = 190/325 (58%), Gaps = 36/325 (11%)
Query: 31 LSDEDNYFRTNPRPVYMDDLLTEATQFIEYQKSQGREKIVLVTSGGTTVPLENNTVRFID 90
+ +++F NP P + D+ + FI Q + + I VTSGGT VPLE NTVRFID
Sbjct: 4 MDKSNDFFEENPCPNSLGDVSSLIEDFINLQSNS--QNIAFVTSGGTLVPLEQNTVRFID 61
Query: 91 NFSAGTRGASSAEQFLFNGYSVIFLHREFSLTPFNRPFIHNTETCFLDYFDEDGS----- 145
NFSAG RGA+SAE F +GYSVIFL+R +SL PF R + E + + F+
Sbjct: 62 NFSAGNRGAASAEYFCKSGYSVIFLYRNYSLMPFTRRY----EGPWTNLFERSNGSQEKP 117
Query: 146 --ISPKYKDLILQNKRDYDQFIYKEKKLLMLPFTTVNQYLWSLKTIAALMN--NSGCLFY 201
+ +++ + + D + K +L+M+P+TT+ +YLW LK IA + S LFY
Sbjct: 118 WIVKQEFQSTVFNALNELDTYT-KHHRLIMIPYTTLTEYLWYLKAIAERLQIIASRALFY 176
Query: 202 LAAAVSDFFVPYSRLPQHKIQSQEANQSLNDGNQSSTTVGGKLIVNLDPVPKFLRRLVES 261
LAAAVSDF +P L +HKIQS + KL V + PVPKFLR LV++
Sbjct: 177 LAAAVSDFHIPPKELSEHKIQSGSGSN--------------KLEVKMHPVPKFLRFLVDT 222
Query: 262 WATQAMIISFKLETDNNLLIHKAHQALDRYNHQLVIGNLLQTRNKEVVFVSEQNRDGDWI 321
WA +A IISFKLETD ++LI KA AL RYNHQLVI NLL TR + V FV+ ++ +W+
Sbjct: 223 WAPEASIISFKLETDPSILIDKARVALQRYNHQLVIANLLSTRKRSVAFVT--SKTVEWL 280
Query: 322 RITDD----HKNIEKLIVQEIVKRH 342
++DD IE+ IV + H
Sbjct: 281 HLSDDDIVKKVEIEQYIVSRAISLH 305
>7300482 [R] KOG2728 Uncharacterized conserved protein with similarity to
phosphopantothenoylcysteine synthetase/decarboxylase
Length = 313
Score = 198 bits (503), Expect = 1e-50
Identities = 128/328 (39%), Positives = 181/328 (55%), Gaps = 32/328 (9%)
Query: 35 DNYFRTNPRPVYMDDLLTEATQFIE-YQKSQGREKIVLVTSGGTTVPLENNTVRFIDNFS 93
++++ T+ P +D + +F E + K Q R IVLVTSGGTTVPLE+NTVRF+DNFS
Sbjct: 5 EDFYNTHLPPADFEDNRSLLKEFCERHNKLQNR--IVLVTSGGTTVPLEHNTVRFVDNFS 62
Query: 94 AGTRGASSAEQFLFNGYSVIFLHREFSLTPFNRPFIHNTETCFLDYFDEDGS----ISPK 149
AGTRG++SAE FL + Y+VIF+HR SL PF R F LD D S I P
Sbjct: 63 AGTRGSASAEYFLDHDYAVIFMHRHKSLEPFTRHFTGQQFFDMLDIADNSQSSTIAIKPD 122
Query: 150 YKDLILQNKRDYDQFIYKEKKLLMLPFTTVNQYLWSLKTIAALMN--NSGCLFYLAAAVS 207
D+ Y + + + +L + FT+V Y+W L+ + + YLAAAVS
Sbjct: 123 SVDVFAPVLAKY-KIARETQMILYVNFTSVVDYMWLLRAACECLAAFEERAVLYLAAAVS 181
Query: 208 DFFVPYSRLPQHKIQSQEANQSLNDGNQSSTTVGGKLIVNLDPVPKFLRRLVESWATQAM 267
DF++P +P HK+QS + G ++L VPK L L W A
Sbjct: 182 DFYIPEDMMPTHKMQSGD----------------GAPTISLQLVPKMLAPLASLWVPHAF 225
Query: 268 IISFKLETDNNLLIHKAHQALDRYNHQLVIGNLLQTRNKEVVFVSEQNRDGDWIRITDDH 327
++SFKLETD +LLI KA +L++Y H+LVI N+LQTR VVFV+ D + +T +
Sbjct: 226 VVSFKLETDESLLIVKARDSLNKYKHKLVIANVLQTRKHRVVFVTP--TDSYELHLTREQ 283
Query: 328 K----NIEKLIVQEIVKRHDAWRKTGKQ 351
IE+ IV ++V++H + +Q
Sbjct: 284 TLQGLEIEEPIVADVVQKHGEFISNAQQ 311
>CE22938 [R] KOG2728 Uncharacterized conserved protein with similarity to
phosphopantothenoylcysteine synthetase/decarboxylase
Length = 277
Score = 179 bits (454), Expect = 5e-45
Identities = 120/287 (41%), Positives = 156/287 (53%), Gaps = 31/287 (10%)
Query: 60 YQKSQGREKIVLVTSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLFNGYSVIFLHREF 119
+ K Q IV +TSGGT VPLE NTVRFIDNFS GTRGA+SAE FL GY+VIF+HRE
Sbjct: 10 FLKKQQNIPIVFITSGGTQVPLEKNTVRFIDNFSMGTRGAASAEYFLRAGYAVIFMHREE 69
Query: 120 SLTPFNRPFIHNTETCFLDYFDEDGSISPKYKDLILQNKRDYDQFIYKEKKLLMLPFTTV 179
SL PF+R F + + LD E+ IL++K Y E+K+L +PF T
Sbjct: 70 SLKPFSRHFPNIFNSLKLD--GENVVCDLPNLAQILRDKFKY------EEKILYIPFKTF 121
Query: 180 NQYLWSLKTIAALMN--NSGCLFYLAAAVSDFFVPYSRLPQHKIQSQEANQSLNDGNQSS 237
+QY+ L+ I +N S + YLAAAVSDF V + LP HKI S
Sbjct: 122 DQYMSRLEQICIHLNPLGSRAMAYLAAAVSDFVV--TELPTHKIASNT------------ 167
Query: 238 TTVGGKLIVNLDPVPKFLRRLVESWATQAMIISFKLETDNNLLIHKAHQALDRYNHQLVI 297
+L + L PK ++++V + A I+SFKLETD LI KA AL +Y HQLVI
Sbjct: 168 -----ELSLELSVAPKVIQKVVTCFIPNAFIVSFKLETDETKLIPKAKAALSKYGHQLVI 222
Query: 298 GNLLQTRNKEVVFVSEQNRDGDWIRITD--DHKNIEKLIVQEIVKRH 342
N+L TR + V V + G+ I + IE I+ + H
Sbjct: 223 ANMLSTRKQRVTLVRKNIEQGEEINLAPGAPELEIESFIIDRVSALH 269
>At5g02080 [R] KOG2728 Uncharacterized conserved protein with similarity to
phosphopantothenoylcysteine synthetase/decarboxylase
Length = 270
Score = 176 bits (446), Expect = 5e-44
Identities = 115/304 (37%), Positives = 161/304 (52%), Gaps = 41/304 (13%)
Query: 47 MDDLLTEATQFIEYQKS-QGREKIVLVTSGGTTVPLENNTVRFIDNFSAGTRGASSAEQF 105
M+++L +FI+ S QG +IV VTSGGTTVPLE VR+IDNFS+G RGA+S E F
Sbjct: 1 MEEILENLNEFIKLNSSSQGGRRIVCVTSGGTTVPLEQRCVRYIDNFSSGNRGAASTENF 60
Query: 106 LFNGYSVIFLHREFSLTPFNRPFIHNTETCFLDYFDEDGSISPKYKDLILQNKRDYDQFI 165
+ GY+VIFL+R + P+ R + FL+ F ++
Sbjct: 61 VKAGYAVIFLYRRGTCQPYCRSL---PDDPFLECF-------------------EFSDKT 98
Query: 166 YKEKKLLMLPFTTVNQYLWSLKTIAALMNNSG--CLFYLAAAVSDFFVPYSRLPQHKIQS 223
+ LL LPF+T+ +YL L+ IA + + G +FYLAAAVSDF+VP+ + +HKI+S
Sbjct: 99 NIQGSLLKLPFSTIYEYLQMLRLIAEALKDVGPCSMFYLAAAVSDFYVPWKSMTEHKIES 158
Query: 224 QEANQSLNDGNQSSTTVGGKLIVNLDPVPKFLRRLVESWATQAMIISFKLETDNNLLIHK 283
G L + L VPK L L +WA +A ISFKLETD+ +L+ K
Sbjct: 159 G----------------SGPLDIRLAQVPKMLSVLRSNWAPKAFCISFKLETDSKILMEK 202
Query: 284 AHQALDRYNHQLVIGNLLQTRNKEVVFVSEQNRDGDWIRITDDHKNIEKLIVQEIVKRHD 343
A +AL +Y V+ N L TR +EVV VS +E +++ IV RH
Sbjct: 203 ATKALRKYKVHAVVANELSTRKEEVVVVSSSGNVVVRRECDKPESFVEDNLIRLIVDRHS 262
Query: 344 AWRK 347
+ K
Sbjct: 263 TYIK 266
>Hs13375919 [R] KOG2728 Uncharacterized conserved protein with similarity to
phosphopantothenoylcysteine synthetase/decarboxylase
Length = 284
Score = 170 bits (430), Expect = 3e-42
Identities = 110/261 (42%), Positives = 151/261 (57%), Gaps = 26/261 (9%)
Query: 63 SQGREKIVLVTSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLFNGYSVIFLHREFSLT 122
+QGR ++VLVTSGGT VPLE VRF+DNFS+G RGA+SAE FL GY V+FL+R S
Sbjct: 32 AQGR-RVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAF 90
Query: 123 PFNRPFIHNTETCFLDYFDE--DGSISPKYKDLIL----QNKRDYDQFIYKEKKLLMLPF 176
P+ F T L G +S + ++ L + R Y + L + F
Sbjct: 91 PYAHRFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAA-AAGTFLAVEF 149
Query: 177 TTVNQYLWSLKTIAALMNNSG--CLFYLAAAVSDFFVPYSRLPQHKIQSQEANQSLNDGN 234
TT+ YL L+ A +N G +FYLAAAVSDF+VP S +P+HKIQS
Sbjct: 150 TTLADYLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSS---------- 199
Query: 235 QSSTTVGGKLIVNLDPVPKFLRRLVESWATQAMIISFKLETDNNLLIHKAHQALDRYNHQ 294
GG L + + VPK L LV+ WA +A IISFKLETD ++I++A +AL+ Y HQ
Sbjct: 200 ------GGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQ 253
Query: 295 LVIGNLLQTRNKEVVFVSEQN 315
+V+ N+L++R V V++ +
Sbjct: 254 VVVANILESRQSFVFIVTKDS 274
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,817,175
Number of Sequences: 60738
Number of extensions: 874813
Number of successful extensions: 2102
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2080
Number of HSP's gapped (non-prelim): 7
length of query: 351
length of database: 30,389,216
effective HSP length: 107
effective length of query: 244
effective length of database: 23,890,250
effective search space: 5829221000
effective search space used: 5829221000
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)