ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV2272    suspect: LH   O     KOG1867  Posttranslational modification, protein turnover, chaperones   Ubiquitin-specific protease

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV2272 784991  787303 771  
         (771 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL122w [O] KOG1867 Ubiquitin-specific protease                       585   e-167

>YDL122w [O] KOG1867 Ubiquitin-specific protease
          Length = 809

 Score =  585 bits (1508), Expect = e-167
 Identities = 308/562 (54%), Positives = 406/562 (71%), Gaps = 19/562 (3%)

Query: 45  VVSTTKEITQAVRDKIMLGRWAPRN--AEQEETIVRRGGYVGGLVNDGNTCFMNSVLQCL 102
           +V +  E+T   R+   L RW PR+     +E I++RGG++ GLVNDGNTCFMNSVLQ L
Sbjct: 60  IVESVSEMTTNFRNNNSLSRWLPRSKFTHLDEEILKRGGFIAGLVNDGNTCFMNSVLQSL 119

Query: 103 ASSKELHKFMDTIILDSNDD-SQNTIDENGEESDLPNEQSTVEKKPKATKNKTYGK-RKK 160
           ASS+EL +F+D  ++ + ++  QN  +E G   +   +++T  KK      K YGK +KK
Sbjct: 120 ASSRELMEFLDNNVIRTYEEIEQNEHNEEGNGQESAQDEAT-HKKNTRKGGKVYGKHKKK 178

Query: 161 IAVRQADNELLKNSTEPNMHFTLALQDLFSKLNAKHYRNRPWFKTNKLLKTMSKAPNRNI 220
           +  + +  E  + S EP++ F++AL+DL S LNAK+YR++P+FKTN LLK MSK+P +NI
Sbjct: 179 LNRKSSSKEDEEKSQEPDITFSVALRDLLSALNAKYYRDKPYFKTNSLLKAMSKSPRKNI 238

Query: 221 ILGYDQEDAQEFFQTILSELEKNVESIYGKPEKDPKPVPQDDLPEDAIVGQEHLGEIGDV 280
           +LGYDQEDAQEFFQ IL+ELE NV+S+    + D  PV + +LP+DA+VGQ +LGE+G V
Sbjct: 239 LLGYDQEDAQEFFQNILAELESNVKSL-NTEKLDTTPVAKSELPDDALVGQLNLGEVGTV 297

Query: 281 YIPTEQIDPNSVLASGR-KFYSPYKIVTPLDGITAERIGCLQCGENGGIRYSVFSGLSLN 339
           YIPTEQIDPNS+L     + ++P+K++TPLDGITAERIGCLQCGENGGIRYSVFSGLSLN
Sbjct: 298 YIPTEQIDPNSILHDKSIQNFTPFKLMTPLDGITAERIGCLQCGENGGIRYSVFSGLSLN 357

Query: 340 LPSDNIGSPIKLSQLLDEWSKPEIIEGVDCNRCALNAVLEHLVEQLNTIQSQPD--VNQK 397
           LP++NIGS +KLSQLL +WSKPEIIEGV+CNRCAL A   HL  QL   + +P+  + +K
Sbjct: 358 LPNENIGSTLKLSQLLSDWSKPEIIEGVECNRCALTAAHSHLFGQLKEFEKKPEGSIPEK 417

Query: 398 LVTLMEDRINEVKSVLSKLAIDDDDYKRMHTENMVRKSSKSKQILISRPPPLLSVHINRS 457
           L+  ++DR+++++ VL+K  IDD+DYK++HT NMVRK SKSKQILISRPPPLLS+HINRS
Sbjct: 418 LINAVKDRVHQIEEVLAKPVIDDEDYKKLHTANMVRKCSKSKQILISRPPPLLSIHINRS 477

Query: 458 VFDPRTYMIRKNNSRVIFKSKLNLSPWCCNYDKINLDARLPMSKKQE-NWHESSEDDNNG 516
           VFDPRTYMIRKNNS+V+FKS+LNL+PWCC+ ++INLDARLPMSKK++    +SSED+N G
Sbjct: 478 VFDPRTYMIRKNNSKVLFKSRLNLAPWCCDINEINLDARLPMSKKEKAAQQDSSEDENIG 537

Query: 517 TEYYNKLHRR---XXXXXXXXXXXXXXXXNGQGMFNGSSRMVEEHDVLNGEXXXXXXXXX 573
            EYY KLH R                   N    +N  ++ +  +D LNGE         
Sbjct: 538 GEYYTKLHERFEQEFEDSEEEKEYDDAEGNYASHYN-HTKDISNYDPLNGE-VDGVTSDD 595

Query: 574 EDDIAEVEEVELDALGNTIVKK 595
           ED+  E    E DALGNTI K+
Sbjct: 596 EDEYIE----ETDALGNTIKKR 613



 Score =  155 bits (392), Expect = 2e-37
 Identities = 71/88 (80%), Positives = 77/88 (86%)

Query: 672 SSVPAGPLTYVLRSVIVHYGTHNYGHYIAFRRFRGCWWRISDESVYIVDEQEVLSTPGVF 731
           S+VPA PLTY LRSVIVHYGTHNYGHYIAFR++RGCWWRISDE+VY+VDE EVLSTPGVF
Sbjct: 672 STVPATPLTYSLRSVIVHYGTHNYGHYIAFRKYRGCWWRISDETVYVVDEAEVLSTPGVF 731

Query: 732 MLFYEYDYDTETGKMKDDTEYENEFNVE 759
           MLFYEYD+D ETGKMKDD E     N E
Sbjct: 732 MLFYEYDFDEETGKMKDDLEAIQSNNEE 759


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.314    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,788,737
Number of Sequences: 60738
Number of extensions: 1884602
Number of successful extensions: 5584
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5574
Number of HSP's gapped (non-prelim): 3
length of query: 771
length of database: 30,389,216
effective HSP length: 114
effective length of query: 657
effective length of database: 23,465,084
effective search space: 15416560188
effective search space used: 15416560188
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)