ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2324 suspect: LH D KOG4575 Cell cycle control, cell division, chromosome partitioning TGc (transglutaminase/protease-like) domain-containing protein involved in cytokinesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2324 809603 807162 -814
(814 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL117w [D] KOG4575 TGc (transglutaminase/protease-like) domain-... 678 0.0
SPAC9G1.06c [D] KOG4575 TGc (transglutaminase/protease-like) dom... 157 5e-38
>YDL117w [D] KOG4575 TGc (transglutaminase/protease-like) domain-containing
protein involved in cytokinesis
Length = 885
Score = 678 bits (1750), Expect = 0.0
Identities = 367/873 (42%), Positives = 531/873 (60%), Gaps = 72/873 (8%)
Query: 9 FKVKTNFAWSGEKKDDLGFLEGDFIEVTRVTGDWYFGKLIRNKK-QGYFPSNYVSILEEQ 67
FKVK + WSG+ K DLGFLEGD +EVTR+ G W++GKL+RNKK GYFP N+V +LEE+
Sbjct: 12 FKVKARYGWSGQTKGDLGFLEGDIMEVTRIAGSWFYGKLLRNKKCSGYFPHNFVILLEER 71
Query: 68 YNTYLPTKHQSN-------------LPRSPQRY--------KEHATSSPEFRKRDQFSLR 106
N+ Q + +P P RY K+ ++S P K+ SL
Sbjct: 72 LNSSTENGRQPSKIVESFEKSNKVVIPPVPSRYSDERPRPKKKLSSSMPNSPKKPVDSLT 131
Query: 107 KNHTNEELAGYSQGKQYSRSFGSKDKYTIPQSHSASNIAKRTGKFQDKYSS--------- 157
K + ++ Y+ + P AS+ + F++ ++
Sbjct: 132 KARKAKSKEMVNEKNIYNTQSSRHHNNSAPNLPLASHSKPQVRNFEESMNNPLPPLPPLP 191
Query: 158 ---------SREXXXXXXXXXXXXXXXXXXFNHISQTGGYTNGMNQPLK-----STDSFG 203
R +N+ + +G + + S G
Sbjct: 192 DLDNMRKTDKRAPKKSYSANDLHMARSSREYNYYKDNQKFYDGFIPEKRYSLEEDSISSG 251
Query: 204 RFSHTRYMEDSLASSEESFAIMSDFSATSAGSFARHNFARSFKDSTDRSMVAEQKDT--- 260
FS+++Y+ DS SSE SFA+MSDFSATSAGSFARH +A+SF DS RS A T
Sbjct: 252 LFSNSQYLNDSACSSENSFALMSDFSATSAGSFARHKYAQSFSDSLQRSQNANGCSTKIN 311
Query: 261 -TSKLGSQ--SMKESKF-DIFKKWL--PGNTKEYNPGAND------YPKLPNIESLNISA 308
+ + G S + K DI +K + NT Y+ + YPKLP+I++LN+SA
Sbjct: 312 DSQEFGDSNASSRNGKMGDILRKIIIPKRNTNIYSSSVSSPKSPKAYPKLPDIQNLNLSA 371
Query: 309 DNHDANSWMEAEAQLRRALTISSNERRERGMRATAKNVDLLLYPHSVVNKDLYSNEVLHT 368
+A W+ + L RA T++ ++ R MRA +N DL+L+P + L +NEV
Sbjct: 372 TPDEARDWIAVKCHLNRARTLTKYDKHPRYMRALEENRDLILHPQDSIYNGLNTNEVKGN 431
Query: 369 RQPGIVDEFLYSADFKRVDKSARQLFFEKLPVQLSVESFAQYSFPSQYFTPLEQLRGLFI 428
+PG+VD L + + +DK + ++++S+AQ +F ++Y T LE+LRG++I
Sbjct: 432 TKPGLVDVELAELNIEYIDKMTWKRCIRD--GTMTLDSWAQTTFSARYSTVLEKLRGIYI 489
Query: 429 FCTELFRLIDDNGKTDFNRSPENLEKALERRYCTPYELTWLFKRMSTALNIKSEIVFGFL 488
FCTE+F L DDNG +DF+ P+NLEK L R++CTPYELTWLFK+++ +L I EIV GFL
Sbjct: 490 FCTEMFALTDDNGTSDFSAEPQNLEKILYRKHCTPYELTWLFKKLANSLGITCEIVIGFL 549
Query: 489 KTPNADNSTFKLNHCWLQVCFYEEWRFVDVILGNITNPIHEYLNNKPAKKCEPFYFLASP 548
KTP+A N FK NHCWL++ +EWRF+DVILGN+TNPIHE++NN+ KK E YFL +P
Sbjct: 550 KTPSAINWEFKYNHCWLRILVNKEWRFIDVILGNVTNPIHEFVNNRKIKKAENSYFLMAP 609
Query: 549 MTLINTHVPREYSKQHIVPELDLNIVLSLPVVFPSFFKNRLRLYRFSNGLCDLEDNEIFE 608
+ +I TH+P +QHIVP +D L LP+VFPSFFKN L+LY+FS L LED+EI+E
Sbjct: 610 LEMIYTHIPPREFEQHIVPSIDQLSALYLPLVFPSFFKNELKLYKFSTALSFLEDSEIYE 669
Query: 609 CTLRIPNDIEVFSNVVV---DEENSLQYKTMNLSLVQVLSQ----NKRNAVIKAVLPPGA 661
C+L IPND+EVF++VV+ +EE S Y+ M L+L Q+ Q +R A+IKAVLPP
Sbjct: 670 CSLEIPNDVEVFASVVIPTDNEEASSAYRNMELALTQIKKQKAESGRRIALIKAVLPPNV 729
Query: 662 SKGTLYIHSGIKGTQCSIENIHPISMILPLTASGNPGNFEFVSRIPSEAVQRVEVYIKEP 721
+KG+LYIHSG++GTQ SI NIHP+SM++PLT G+ +EFV +IPSE++Q++E+YI EP
Sbjct: 730 NKGSLYIHSGVRGTQTSIANIHPLSMMVPLTHKGSNMKYEFVIKIPSESIQKIELYIVEP 789
Query: 722 QNKFLSNKRKYKFEMIQNPTDGIIHQS---MDRNLSRAMLILSPTGKRYKVIKNDPHLPF 778
Q+++L +Y FE+IQ+P+DGI++ S ++N + M I SP+G+ ++++K+DPH P+
Sbjct: 790 QSRYLFVGNEYSFEVIQSPSDGIVYSSDEGPNQNRKQPMAIKSPSGRVHELVKSDPHFPY 849
Query: 779 GTWSASIEINETGRWNAMIISDAGSGWCEFAQW 811
GTW SI+I E G W+A++I+D+G GW FA+W
Sbjct: 850 GTWKGSIKIKEPGVWSALVIADSGIGWSVFAEW 882
>SPAC9G1.06c [D] KOG4575 TGc (transglutaminase/protease-like) domain-containing
protein involved in cytokinesis
Length = 886
Score = 157 bits (398), Expect = 5e-38
Identities = 123/444 (27%), Positives = 204/444 (45%), Gaps = 22/444 (4%)
Query: 378 LYSADFKRVDKSARQLFFEKLPVQLSVESFAQYSFPSQYFTPLEQLRGLFIFCTELFRLI 437
+++ +F +DK R + +++ V +V + Y P Q T L Q+R +FI+ +E R+
Sbjct: 456 IHNMNFSAIDKQIRSIIPQRVQVSPAVLA-KNYLAPGQT-TALAQMRAVFIYISE--RIT 511
Query: 438 DDNGKTDFNRSPENLEKALERRYCTPYELTWLFKRMSTALNIKSEIVFGFLKTPNADNST 497
N D N + + TP+E+ L K M AL++ E++ G+LK+P+ T
Sbjct: 512 FTNQTLD-NDELRTSTQVISEGQGTPFEVALLVKEMLQALDLWCEVIEGYLKSPDDIYYT 570
Query: 498 --FKLNHCWLQVCFYEEWRFVDVILGNITNPIHEYLNNKPAKKCEPFYFLASPMTLINTH 555
+NH W V F E R +D + T+P ++ FYFL P I TH
Sbjct: 571 RDININHAWNVVTFDNEVRLIDASFASPTHPQQALKSSSS----NDFYFLMKPNECIFTH 626
Query: 556 VPREYSKQHIVPELDLNIVLSLPVVFPSFFKNRLRLYRFSNGLCDLEDNEIFECTLRIPN 615
VP +Q I+P+L + IV++LP V +F L+L +F+ + L D E+ + P
Sbjct: 627 VPENPDQQFIMPDLSMPIVMALPWVSSVYFTLGLKLRKFNTSILHLNDLEVLQIEFLAPK 686
Query: 616 DIEVFSNV-----VVDEENSLQYKTMNLSLVQVLSQNKRNAVIKAVLPPGASKGTLYIHS 670
DIE + V + + Q L+ + + R +KAV+P L I++
Sbjct: 687 DIECVAEVDALSALAPTADVSQCYKYTLTQAFWETPDIRVMRVKAVMPANNRAAVLRIYA 746
Query: 671 GIKGTQCSIENI-HPISMILPLTASGNPGNFEFVSRIPSEAVQRVEVYIKEPQNKFLSNK 729
G G + HP++M LP G EFV+R P V++YI PQ L +
Sbjct: 747 GRLGVSSPVRTAPHPMAMSLPFVHHGKNKALEFVTRHPVPHCPSVDLYINSPQCGTLHSG 806
Query: 730 RKYKFEMIQNPTDGIIHQSMDRNLSRAMLILSPTGKRYKVIKNDPHLPFGTWSASIEINE 789
+YKF + Q + + I +PTG ++ + +S S+ INE
Sbjct: 807 VEYKFNV-----SAYACQPSTSISNTRLAIQTPTGNIVRLREERSGNGVIFFSLSLTINE 861
Query: 790 TGRWNAMIISDAGSGWCEFAQWES 813
TG + A+I+++ W +A W++
Sbjct: 862 TGEYRALILAEKIGRWVVYATWQA 885
Score = 57.0 bits (136), Expect = 1e-07
Identities = 33/85 (38%), Positives = 42/85 (48%), Gaps = 3/85 (3%)
Query: 11 VKTNFAWSGEKKDDLGFLEGDFIEVTRV-TGDWYFGKLIRNKKQGYFPSNYVSILEEQYN 69
V+ +AW GE++ DL F EGD IE + G W+ G+ I QG FPSN+V L+
Sbjct: 11 VRALYAWPGEREGDLKFTEGDLIECLSIGDGKWWIGRHINTNTQGIFPSNFVHCLD--IP 68
Query: 70 TYLPTKHQSNLPRSPQRYKEHATSS 94
T P S S RY SS
Sbjct: 69 TVRPGSSMSRTSASSFRYSSPQKSS 93
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,224,923
Number of Sequences: 60738
Number of extensions: 2222057
Number of successful extensions: 5731
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5716
Number of HSP's gapped (non-prelim): 3
length of query: 814
length of database: 30,389,216
effective HSP length: 115
effective length of query: 699
effective length of database: 23,404,346
effective search space: 16359637854
effective search space used: 16359637854
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)