ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2330 good E KOG4492 Amino acid transport and metabolism Chorismate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2330 811468 810341 -376
(376 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL148w [E] KOG4492 Chorismate synthase 578 e-165
At1g48850 [E] KOG4492 Chorismate synthase 301 8e-82
SPCC1223.14 [E] KOG4492 Chorismate synthase 209 5e-54
>YGL148w [E] KOG4492 Chorismate synthase
Length = 376
Score = 578 bits (1490), Expect = e-165
Identities = 285/375 (76%), Positives = 310/375 (82%)
Query: 1 MSTFGQIFRVTTYGESHCKSVGCIVDGVPPGMSLTEDDIQPQLTRRRPGQSKLSTPRNEK 60
MSTFG++FRVTTYGESHCKSVGCIVDGVPPGMSLTE DIQPQLTRRRPGQSKLSTPR+EK
Sbjct: 1 MSTFGKLFRVTTYGESHCKSVGCIVDGVPPGMSLTEADIQPQLTRRRPGQSKLSTPRDEK 60
Query: 61 DRVEIQSGTEFGKTLGTPIGMLVKNEDQRPHDYSDMDNYPRPSHADFTYSSKYGIKXXXX 120
DRVEIQSGTEFGKTLGTPI M++KNEDQRPHDYSDMD +PRPSHADFTYS KYGIK
Sbjct: 61 DRVEIQSGTEFGKTLGTPIAMMIKNEDQRPHDYSDMDKFPRPSHADFTYSEKYGIKASSG 120
Query: 121 XXXXXXXETIGRVAAGAIAEKFLKQLSNVEIVAFVTQIGAVKMNRDPQDPKFQQVLNTIT 180
ETIGRVA+GAIAEKFL Q SNVEIVAFVTQIG +KMNRD DP+FQ +LNTIT
Sbjct: 121 GGRASARETIGRVASGAIAEKFLAQNSNVEIVAFVTQIGEIKMNRDSFDPEFQHLLNTIT 180
Query: 181 REKVDAAGPIRCPDSSVADAMVKEIERYRGEKDSIGGVVTCVIRNLPAGLGEPCFDKLEA 240
REKVD+ GPIRCPD+SVA MVKEIE+YRG KDSIGGVVTCV+RNLP GLGEPCFDKLEA
Sbjct: 181 REKVDSMGPIRCPDASVAGLMVKEIEKYRGNKDSIGGVVTCVVRNLPTGLGEPCFDKLEA 240
Query: 241 LLAHAMLSIPASKGFEIGSGFDGVCLPGSKHNDPFYFDEDTGRLRTKTXXXXXXXXXXXX 300
+LAHAMLSIPASKGFEIGSGF GV +PGSKHNDPFYF+++T RLRTKT
Sbjct: 241 MLAHAMLSIPASKGFEIGSGFQGVSVPGSKHNDPFYFEKETNRLRTKTNNSGGVQGGISN 300
Query: 301 XXXXYFSVPFKSAATISQEQATATYDGKSGILAAKGRHDPSVTPRATPIXXXXXXXXXXX 360
YFSVPFKS ATISQEQ TATYDG+ GILAAKGRHDP+VTPRA PI
Sbjct: 301 GENIYFSVPFKSVATISQEQKTATYDGEEGILAAKGRHDPAVTPRAIPIVEAMTALVLAD 360
Query: 361 XXXIQKSREAAISIV 375
IQK+R+ + S+V
Sbjct: 361 ALLIQKARDFSRSVV 375
>At1g48850 [E] KOG4492 Chorismate synthase
Length = 435
Score = 301 bits (772), Expect = 8e-82
Identities = 164/351 (46%), Positives = 213/351 (59%), Gaps = 15/351 (4%)
Query: 2 STFGQIFRVTTYGESHCKSVGCIVDGVPPGMSLTEDDIQPQLTRRRPGQSKLSTPRNEKD 61
S++G FRV+T+GESH VGCI+DG PP + LTE D+Q L RR PGQS+++TPR E D
Sbjct: 53 SSYGTHFRVSTFGESHGGGVGCIIDGCPPRIPLTESDLQFDLDRR-PGQSRITTPRKETD 111
Query: 62 RVEIQSGTEFGKTLGTPIGMLVKNEDQRPHDYSDMDNYPRPSHADFTYSSKYGIKXXXXX 121
I SG G T GTPI + V N DQR DYS+M RPSHAD TY KYG++
Sbjct: 112 TCRISSGVSEGMTTGTPIHVFVPNTDQRGLDYSEMSVAYRPSHADATYDMKYGVRSVQGG 171
Query: 122 XXXXXXETIGRVAAGAIAEKFLKQLSNVEIVAFVTQIGAVKMNR---DPQDPKFQQVLNT 178
ETIGRVA GA+A+K LKQ + EI+A+V+Q+ V + D ++ +Q+ N
Sbjct: 172 GRSSARETIGRVAPGALAKKILKQFAGTEILAYVSQVHHVVLPEELVDHENLTLEQIENN 231
Query: 179 ITREKVDAAGPIRCPDSSVADAMVKEIERYRGEKDSIGGVVTCVIRNLPAGLGEPCFDKL 238
I +RCP+ A+ M+ I+ R + +S+GGVVTC++RN P GLG P FDKL
Sbjct: 232 I----------VRCPNPEYAEKMIAAIDAVRTKGNSVGGVVTCIVRNAPRGLGTPVFDKL 281
Query: 239 EALLAHAMLSIPASKGFEIGSGFDGVCLPGSKHNDPFYFDEDTGRLRTKTXXXXXXXXXX 298
EA LA A +S+PA+KGFE GSGF G L G +HND FY DE+ GR+RT+T
Sbjct: 282 EAELAKACMSLPATKGFEFGSGFAGTFLTGLEHNDEFYTDEN-GRIRTRTNRSGGIQGGI 340
Query: 299 XXXXXXYFSVPFKSAATISQEQATATYDGKSGILAAKGRHDPSVTPRATPI 349
V FK +TI ++Q T T D + A+GRHDP V PRA P+
Sbjct: 341 SNGEIINMRVAFKPTSTIGRKQNTVTRDKVETEMIARGRHDPCVVPRAVPM 391
>SPCC1223.14 [E] KOG4492 Chorismate synthase
Length = 124
Score = 209 bits (532), Expect = 5e-54
Identities = 94/116 (81%), Positives = 107/116 (92%)
Query: 1 MSTFGQIFRVTTYGESHCKSVGCIVDGVPPGMSLTEDDIQPQLTRRRPGQSKLSTPRNEK 60
MS+FG +F+VTTYGESHCKSVGCIV+G PPGM+LTE D+Q QLTRRRPGQS L+TPRNEK
Sbjct: 1 MSSFGTLFKVTTYGESHCKSVGCIVEGCPPGMNLTESDVQVQLTRRRPGQSNLTTPRNEK 60
Query: 61 DRVEIQSGTEFGKTLGTPIGMLVKNEDQRPHDYSDMDNYPRPSHADFTYSSKYGIK 116
D+V+IQSGTEFG TLGTPIGMLV N+DQ+PHDYSDMDNYPRPSHAD+TY KYG+K
Sbjct: 61 DKVQIQSGTEFGVTLGTPIGMLVLNQDQKPHDYSDMDNYPRPSHADYTYLEKYGVK 116
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,226,245
Number of Sequences: 60738
Number of extensions: 809115
Number of successful extensions: 1754
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1748
Number of HSP's gapped (non-prelim): 3
length of query: 376
length of database: 30,389,216
effective HSP length: 108
effective length of query: 268
effective length of database: 23,829,512
effective search space: 6386309216
effective search space used: 6386309216
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)