ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactV2346    suspect: LH   S     KOG4852  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactV2346 816633 815509 -375 
         (375 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL115c [S] KOG4852 Uncharacterized conserved protein                 128   2e-29
7298630 [S] KOG4852 Uncharacterized conserved protein                  55   2e-07

>YDL115c [S] KOG4852 Uncharacterized conserved protein
          Length = 240

 Score =  128 bits (321), Expect = 2e-29
 Identities = 89/265 (33%), Positives = 122/265 (45%), Gaps = 32/265 (12%)

Query: 116 MLNDYLKI-KEKDSPEXXXXXXXXXXXXXYTAPILPPTDYVYDVYVKQTIPEDEEEFVFD 174
           M+NDYLK+ K  +  E                  LP  DYV+D+Y  + I +DE     +
Sbjct: 1   MVNDYLKLNKGVEKTERKKPSRKYFTGDSAKIASLPSLDYVFDIYHLEKIHDDEVARYNN 60

Query: 175 KSTIGYIRIVEHNGDMIPEEEVDPEKTMLTDDEDSNEEDYYQNDYPEDEDDDRSIFIGSD 234
           +  IG+++I+EH  D+  +EE DP +   +DDEDSN+E+YYQNDYPEDEDDDRSI  GS 
Sbjct: 61  EKNIGFVKIIEHI-DLALDEESDPNEAR-SDDEDSNDENYYQNDYPEDEDDDRSILFGS- 117

Query: 235 EDRTDSEDIVYLDE-----LNDELDQNYNVDGEQANETTNDNGDEFDALFQNYGEXXXXX 289
               + EDI  L E     +N     ++N D  Q +   +D  +E+  LF   G      
Sbjct: 118 ----EGEDIAALGEEIVIGVNKSRFSSWNDDKIQGSNGYHDVEEEYGDLFNRLGGKSDVL 173

Query: 290 XXXXXXXXXXXXXXXXXXXXXXXXXGKYDGDFVNLKPEAXXXXXXXXXXXXQTFKRNYFF 349
                                       +GD +                    + RN FF
Sbjct: 174 KSINSSNFIDLDGQEGEIEISDNEDDSDEGDDIE-------------------YPRNEFF 214

Query: 350 PTDKDDEDAIHRDRIFGKLQKMIDK 374
           PTD DD  A HRDRIF +LQK I++
Sbjct: 215 PTDVDDPLAHHRDRIFHQLQKKINR 239


>7298630 [S] KOG4852 Uncharacterized conserved protein
          Length = 367

 Score = 54.7 bits (130), Expect = 2e-07
 Identities = 53/230 (23%), Positives = 92/230 (39%), Gaps = 47/230 (20%)

Query: 84  NNDKKMVYVLERERERQAASE---------------------LQEELPDSIAEMLNDYLK 122
           +ND  +   L R+R+RQ A E                      + E P+S +   + ++ 
Sbjct: 96  SNDAAIASALRRDRQRQEAQENSREQRFRVVNCLRSTLENSAAEAECPESQSHESSSHIT 155

Query: 123 IKEKDSPEXXXXXXXXXXXXXYTAPILPPTD-----------YVYDVYVKQTIPEDEEEF 171
           I + +S +                  + P+            YVYD+YV    PE+E + 
Sbjct: 156 IVDIESQQQQHMGTASAAAEEQQQQEIAPSQDQQQPADSDVGYVYDLYV----PENEMQA 211

Query: 172 VF-DKSTIGYIRIVEHNGDMIPEEEVDPEKTMLTDDEDSNEEDYYQNDYPEDEDDDRSIF 230
            + D     Y+ ++     ++ +   D ++    D EDSN+E+Y+ NDYP   DDD +  
Sbjct: 212 AYVDMMDDNYLSVIPVGEIVLEDCYNDQDEDY--DSEDSNQENYFTNDYP---DDDEAGA 266

Query: 231 IGSDEDRTDSEDIVYLDELNDEL-----DQNYNVDGEQANETTNDNGDEF 275
           +GSD++     +   LD+  DE      D +Y    +    T +   D F
Sbjct: 267 MGSDDELCRQMNKFMLDDDEDEFASTSDDDDYATFRDPYVHTIDTEEDSF 316


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.309    0.133    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,974,221
Number of Sequences: 60738
Number of extensions: 1028461
Number of successful extensions: 7980
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 7969
Number of HSP's gapped (non-prelim): 6
length of query: 375
length of database: 30,389,216
effective HSP length: 108
effective length of query: 267
effective length of database: 23,829,512
effective search space: 6362479704
effective search space used: 6362479704
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)