ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2348 suspect: Pn Q KOG1201 Secondary metabolites biosynthesis, transport and catabolism Hydroxysteroid 17-beta dehydrogenase 11
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2348 816861 817700 280
(280 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL114w [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11 57 2e-08
CE28011 [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11 57 4e-08
7297912 [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11 50 3e-06
Hs20373187 [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11 49 6e-06
YLR426w [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11 49 8e-06
SPCC162.03 [Q] KOG1205 Predicted dehydrogenase 49 8e-06
>YDL114w [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11
Length = 308
Score = 57.4 bits (137), Expect = 2e-08
Identities = 54/248 (21%), Positives = 96/248 (37%), Gaps = 12/248 (4%)
Query: 42 VVAGGSRGIGSHLVTMFDSLGYKTILID-KRYP-LNLIEPHRVSFYKCDISDFEYTRLIL 99
++ GGS G+G L K I+ D + +P +E + + +Y+CDI+ + + +
Sbjct: 42 LITGGSSGLGFELAKELSRRINKVIVADIQSFPTFAQVEYNNIFYYQCDITSLDEIKNLK 101
Query: 100 DDISVRFGQITVFINAVSLKSFTDAEVGKGVSLRKLMRTVYAGSMHIIQHILPKMIAYDR 159
I G I + IN + E + +L+ G+ II MI
Sbjct: 102 KAIERDHGNINIIINNAGVAHIKKLEHMTNKEVEQLIDINLIGAYRIISTFAEDMIDNRE 161
Query: 160 GYIIDIASTEGLYTTENKSSYGAIQATKFQLLQLIEQTLKEQYK-------KNFLISNGR 212
G+II+IAS G T +SYGA + + + + + K L+ G+
Sbjct: 162 GFIINIASVLGELTPARLTSYGASKGAMIGFHKCMSRHFRSLSTECNKTGIKTLLVCPGK 221
Query: 213 VRCLLLIFGKALNSKPHQDM---TTAKTVFSAIFHNRQGVQGYSMRTNFKLALRELDWQW 269
++ + I + D+ A + SA+ HN N + L W +
Sbjct: 222 IKTNMFIDVPTPSKLLAPDIIPSQLALAIISAMEHNHLQTLNAPYYVNLVPFFKTLSWPY 281
Query: 270 YQMFKYLS 277
+ K+ S
Sbjct: 282 RHLLKHFS 289
>CE28011 [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11
Length = 427
Score = 56.6 bits (135), Expect = 4e-08
Identities = 44/169 (26%), Positives = 74/169 (43%), Gaps = 15/169 (8%)
Query: 37 QKDIVVVAGGSRGIGSHLVTMFDSLGYKTILID--KRYPLNLI-----EPHRVSFYKCDI 89
Q V++ GG G+G + F K +ID K L + E + F+ CDI
Sbjct: 40 QGQTVIITGGGSGLGRAMALDFAKRKAKVAIIDVNKEGGLETVKTIAAEGNMAKFWYCDI 99
Query: 90 SDFEYTRLILDDISVRFGQITVFINAVSLKSFTDAEVGKGVSLRKLMRTVYAGSMHIIQH 149
SD + + +I FG + + I ++ SFT LRK + G+++ I+
Sbjct: 100 SDVDNMKKTAKEIEDTFGDVNIVICNAAILSFTSFMEISDELLRKCLDVNIFGTINTIRA 159
Query: 150 ILPKMIAYDRGYIIDIASTEG--------LYTTENKSSYGAIQATKFQL 190
LPKM + G+I+ + S G Y T + GA+++ + +L
Sbjct: 160 FLPKMETKNDGHIVCVCSIAGWSGETMGLSYCTSKFAVRGAMESLQMEL 208
>7297912 [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11
Length = 299
Score = 50.4 bits (119), Expect = 3e-06
Identities = 57/195 (29%), Positives = 80/195 (40%), Gaps = 42/195 (21%)
Query: 18 YVRVLEDLVTSFVGIPLCPQK------DIVVVAGGSRGIGSHLVTMFDSLGYKTILID-- 69
YVR+L +L S V I L P+K +IV++ G GIG L + SLG + +D
Sbjct: 29 YVRILLELFVSLVQIVL-PKKQKDVSGEIVLITGTGHGIGRELALHYASLGSTVVCVDID 87
Query: 70 ---------KRYPLNLIEPHRVSFYKCDISDFEYTRLILDDISVRFGQITVFINAVSLKS 120
K LNL E V Y CD+S + + D I G I+V +N V +
Sbjct: 88 GKNNLQTVEKAKRLNLGE---VYSYSCDVSKRDEVTALADRIKSDVGCISVLVNNVGIMP 144
Query: 121 FTDAEVGKGVSLRKLMRTVYAGSMHIIQHILPKMIAYDRGYIIDIASTEGLYTTENKSSY 180
+ S IQ + + RG+II ++S GL N Y
Sbjct: 145 ---------------THPILQQSAEEIQRVFDEKC---RGHIICMSSIAGLVGISNLVPY 186
Query: 181 GAIQATKFQLLQLIE 195
ATKF + L+E
Sbjct: 187 ---CATKFAVRGLME 198
>Hs20373187 [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11
Length = 231
Score = 49.3 bits (116), Expect = 6e-06
Identities = 44/175 (25%), Positives = 67/175 (38%), Gaps = 17/175 (9%)
Query: 39 DIVVVAGGSRGIGSHLVTMFDSLGYKTILIDKRYPLNL--------IEPHRVSFYKCDIS 90
+IV++ G G+G L F LG +L D N RV Y CD S
Sbjct: 41 EIVLITGAGSGLGRLLALQFARLGSVLVLWDINKEGNEETCKMAREAGATRVHAYTCDCS 100
Query: 91 DFEYTRLILDDISVRFGQITVFINAVSL---KSFTDAEVGKGVSLRKLMRTVYAGSMHII 147
E + D + G +++ IN + K F D + K + +
Sbjct: 101 QKEGVYRVADQVKKEVGDVSILINNAGIVTGKKFLDCP---DELMEKSFDVNFKAHLWTY 157
Query: 148 QHILPKMIAYDRGYIIDIASTEGLYTTENKSSYGAIQATKFQLLQLIEQTLKEQY 202
+ LP MIA D G+++ I+S+ GL + Y A+KF E E +
Sbjct: 158 KAFLPAMIANDHGHLVCISSSAGLSGVNGLADY---CASKFAAFGFAESVFVETF 209
>YLR426w [Q] KOG1201 Hydroxysteroid 17-beta dehydrogenase 11
Length = 326
Score = 48.9 bits (115), Expect = 8e-06
Identities = 49/206 (23%), Positives = 95/206 (45%), Gaps = 12/206 (5%)
Query: 40 IVVVAGGSRGIGSHLVT-MFDSLGYKTILIDKRYPLNLIEPHRVSFYKCDISDFEYTRLI 98
IV++ GGS+G+G +V+ + TIL P + + RV CD+SD E +
Sbjct: 72 IVLITGGSKGLGRAIVSQLLQDYSNLTILNVDICPSS-VRNTRVKDLICDLSDDEEVAAL 130
Query: 99 LDDISVRF-GQITVFINAVSLKS-FTDAEVGKGVSLRKLMRTVYAGSMHIIQHILPKMIA 156
L+ + ++ +I + +N +++ FT + +L K+ + + IQ + P +
Sbjct: 131 LNLLKRKYKNEIRLIVNNAGVRANFTGFNGMERDNLDKIFKINTFAPLQFIQELAPSRHS 190
Query: 157 YDRGYIIDIASTEGLYTTENKSSYGAIQATKFQLLQLIEQTLKEQYKKNFLISNGRVRCL 216
+ YI++IAS G+ T ++Y A +A Q L+ + +N +R L
Sbjct: 191 TRQCYIVNIASILGILTPAKVAAYAASKAALIAFHQSYSFELQNEGVRN-------IRTL 243
Query: 217 LLIFGKALNSKPHQDMTTAKTVFSAI 242
L+ G+ LN++ + F+ +
Sbjct: 244 LVTPGQ-LNTEMFAGFKPPRQFFAPV 268
>SPCC162.03 [Q] KOG1205 Predicted dehydrogenase
Length = 292
Score = 48.9 bits (115), Expect = 8e-06
Identities = 42/170 (24%), Positives = 74/170 (42%), Gaps = 5/170 (2%)
Query: 41 VVVAGGSRGIGSHLVTMFDSLGYKTILIDKRYPLNLIEPHRVSFYKCDISDFEYTRLILD 100
V++ G S+G+G LV + + GY I + IE ++ K D++D +
Sbjct: 8 VLITGSSKGLGYALVKVGLAQGYNVIACSRAPDTITIEHSKLLKLKLDVTDVKSVETAFK 67
Query: 101 DISVRFGQITVFINAVSLKSFTDAEVGKGVSLRKLMRTVYAGSMHIIQHILPKMIAYDR- 159
D RFG + + IN + E + + M + G +I + L M +
Sbjct: 68 DAKRRFGNVDIVINNAGYGLVGEFESYNIEEMHRQMNVNFWGVAYITKEALNLMRESGKG 127
Query: 160 GYIIDIASTEGLYTTENKSSYGAIQATKFQLLQLIEQTLKEQYKKNFLIS 209
G I+ I+S G Y + S Y A+KF ++ + QT+ + N+ I+
Sbjct: 128 GRILQISSVAGYYPSPCLSMY---NASKF-AVEGLSQTIMRELDPNWNIA 173
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.140 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,594,368
Number of Sequences: 60738
Number of extensions: 609066
Number of successful extensions: 1916
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1910
Number of HSP's gapped (non-prelim): 6
length of query: 280
length of database: 30,389,216
effective HSP length: 105
effective length of query: 175
effective length of database: 24,011,726
effective search space: 4202052050
effective search space used: 4202052050
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)