ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2352 good U KOG2273 Intracellular trafficking, secretion, and vesicular transport Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2352 819739 817832 -636
(636 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL113c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/... 506 e-143
YDR425w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/... 127 7e-29
SPBC14F5.11c [U] KOG2273 Membrane coat complex Retromer subunit ... 84 9e-16
SPCC16A11.08 [U] KOG2273 Membrane coat complex Retromer subunit ... 72 3e-12
>YDL113c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 640
Score = 506 bits (1304), Expect = e-143
Identities = 275/623 (44%), Positives = 391/623 (62%), Gaps = 21/623 (3%)
Query: 25 NNKTVEEHKEHEPDLQTQ----SEMRRESNGSPKDTAVTNQNGDQPHENV----IHTQII 76
N++T K P T+ +E+ + GSPK + + G + V +HT ++
Sbjct: 14 NSETRNTGKAEPPHGTTEYVAEAEISKNGVGSPKKSPKKGKVGKGDNNKVETELVHTALL 73
Query: 77 KKDNPFFNYMQDNVDGSXXXXXXXXXXXXXXXXXSDDNED-QLLQPXXXXXXXXXXXXXV 135
+KDNPF S D +D + L P +
Sbjct: 74 EKDNPFMEEGPTGFTKSALLEIPGMRSHNLKNPNEDYEDDSEGLLPLNQESNAETCRTSL 133
Query: 136 ATSKDSSPDVPYNTLNHNASGMTKEGKKRAQILEASKVSEGQGRTYIAYAIKYGDSIVKR 195
+ S +S + + S ++ R ILEA +VSEGQGR YIAY I++ +S V+R
Sbjct: 134 SGSINSMNGE--TSASEEPSVSNRKKSARIHILEAKRVSEGQGRAYIAYVIQFENSTVQR 191
Query: 196 RYSDFESLRKVLVKLFPISLIPPIPEKQSLKSYGKAMTYSKSSYLLPTESGDSVDLSLSV 255
RYSDFESLR +L++LFP++LIPPIPEKQS+K+YGK++T S S YLLP+E SVDLSLSV
Sbjct: 192 RYSDFESLRSILIRLFPMTLIPPIPEKQSIKNYGKSITGSSSKYLLPSEGSGSVDLSLSV 251
Query: 256 INGPVTTNDEKLIRHRIRMLTSFLNRLLKNQEITKTSIVYDFLDPNNKNWNDXXXXXXXX 315
I+ V +DEKLIRHRIRMLT FLN+LL N+EITKTSI+ DFLDPNN NW++
Sbjct: 252 IHASVNNSDEKLIRHRIRMLTEFLNKLLTNEEITKTSIITDFLDPNNHNWHEFVNSSSTF 311
Query: 316 XXXPKSVLQCNPIDPTNTTKAHSYLPVPSSSTQLLASKDNHSASDADE----FTKIEAEF 371
PKS+LQCNP+DPTNTT+ H+ LP+P SS+QLL +K+++ E FT IE ++
Sbjct: 312 SSLPKSILQCNPLDPTNTTRIHAMLPIPGSSSQLLLNKESNDKKMDKERSKSFTNIEQDY 371
Query: 372 KNYEQLIHSGLYKYSRATTKEVNYLREDLKGISSEFAQLSTDETKNESGLAELLSHSSDA 431
K YE L+ +G+YKY+R TTK + L+ D I FAQ + ++ + LAE LS+ S+A
Sbjct: 372 KQYENLLDNGIYKYNRRTTKTYHDLKSDYNEIGEVFAQFAHEQAQ-VGELAEQLSYLSNA 430
Query: 432 YGTLQEILETLVGNLHYNINEPLSECAHMATAVRDLIHYRRLKLIQKDILERTILYKRGQ 491
+ LE LVG L+YNINEPL+E HMAT+ R+LI YR+LK +Q +++++++ KR Q
Sbjct: 431 FSGSSISLEKLVGRLYYNINEPLNESVHMATSARELIKYRKLKYLQNEMIKKSLNSKRAQ 490
Query: 492 LIKFQQQENDHKQIDNMVKEELGTNGAVNLENP-----AGPQSYSGKFINKFTQLAIIIK 546
L K + Q N++K +D ++ E+ + +NLE P +G +SY GK N F +LA ++K
Sbjct: 491 LEKLEAQNNEYKDVDKIIDNEMSKSHTINLERPNNNTGSGGKSYGGKLFNGFNKLASMVK 550
Query: 547 DSVSYQEQDPAAAARSLEKELIQLDETLKVATSDLVVISETLKNTELPNFIKERDEELTQ 606
DSV YQE DP A+ +L+KE+ QL E+L+V +DL VIS+ +KN +LP F KER+ +L++
Sbjct: 551 DSVKYQETDPHTASINLKKEIEQLSESLEVTENDLEVISKVIKNDQLPKFSKEREVDLSE 610
Query: 607 IFKNYAKYMKENATRNLEIWKEL 629
I K+Y++YM+ A +NLEIWKE+
Sbjct: 611 ILKHYSRYMRNYARQNLEIWKEV 633
>YDR425w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 625
Score = 127 bits (318), Expect = 7e-29
Identities = 129/545 (23%), Positives = 227/545 (40%), Gaps = 107/545 (19%)
Query: 161 GKKRAQILEASKVSEGQGRTYIAYAIKYGDSIVKRRYSDFESLRKVLVKLFPISLIPPIP 220
G ++ QI++A + G+ I Y I Y ++ + RRYS+F SLR+ L +L P +IPPIP
Sbjct: 118 GTQQIQIIDAGDFKDPWGKHAIGYVILYENNKIIRRYSEFHSLRQSLTRLLPTIIIPPIP 177
Query: 221 EKQSLKSYGKAMTYSKSSYLLPTESGDSVDLSLSVINGPVTT-NDEKLIRHRIRMLTSFL 279
K SL Y I P+ ND K+I R +ML SFL
Sbjct: 178 SKHSLLKY---------------------------IWSPINAANDSKIISTRKKMLNSFL 210
Query: 280 NRLLKNQEITKTSIVYDFLDPNNKNWNDXXXXXXXXXXXPKSVLQCNPIDPTNTTKAHSY 339
+ L QEI+ + FL+P NW D P + L P+ PT + HS
Sbjct: 211 SNCLNIQEISNDIVFQKFLNP-EFNWKD-VLSSSPIIILPLNNLLAPPLSPTKPSPLHSI 268
Query: 340 LPVPSSST---------QLLASKDNHSAS--------DADEFTKIEAEFKNYEQLIHSGL 382
LP+PS+S+ Q + K ++ S + +FT IE F+NY+++I L
Sbjct: 269 LPIPSNSSLRNYNSIWQQHITVKSHNEISNLPTEILQNESQFTHIENLFQNYKRIITHLL 328
Query: 383 YKYSRATTKEVNYLREDLKGISSEFAQL-----------------STDETKNESG--LAE 423
K + + +S+ FA+L S E++N S + E
Sbjct: 329 --------KNIRSNKSHFHSLSTYFAELGAYYNAFSLENDITMPNSLRESENNSNNPMME 380
Query: 424 LLSH---SSDAYGTLQEILETLVGNLHYNINEPLSECAHMATAVRDLIHYRRLKLIQKDI 480
++SH + ++ + E L+ + +P++E ++++++LK +Q I
Sbjct: 381 IISHIEKTGHSFDVIYISSEILIEKYTSILEDPINELLQFLNESFKVLNFKKLKFLQFKI 440
Query: 481 LERTILYKRGQLIKFQQQENDHKQIDNMVKEELGTNGAVNLENPAGPQSYSGKFINKFTQ 540
LER I+ K +L + EN ++I+ + + A ++ K + ++
Sbjct: 441 LERLIIEKETKLSSLTEIENQLQKINESLTRSTILTDENYKDTKAADLTFVKKDVRSLSK 500
Query: 541 LAI------------IIKDSVSYQ-----------------EQDPAAAARSLEKELIQLD 571
+ I ++Y+ EQ+ + + L ++L +L
Sbjct: 501 SSSNSSSSGHQNEIHIGASKLNYKTSTPTMNLNKLEIKQLTEQERSKQIKQLNQDLSKLK 560
Query: 572 ETLKVATSDLVVISETLKNTELPNFIKERDEELTQIFKNYAKYMKENATRNLEIWKELHN 631
+ L + SD++ I+ + N+ L + + + +I K +A K L+ WK
Sbjct: 561 DCLSICISDMLEINNSSYNS-LMHTYNHINLTIGKILKLFAASFKAWIKECLKNWKLAKL 619
Query: 632 RIQEA 636
+I EA
Sbjct: 620 QIDEA 624
>SPBC14F5.11c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 586
Score = 83.6 bits (205), Expect = 9e-16
Identities = 76/339 (22%), Positives = 142/339 (41%), Gaps = 38/339 (11%)
Query: 175 EGQGRTYIAYAIKYGDSIVKRRYSDFESLRKVLVKLFPISLIPPIPEKQSLKSYGKAMTY 234
+ + ++I Y IK DS + RYS+F SLR L +L+P L+PP+P+K + Y
Sbjct: 82 KAESGSHIVYIIKLQDSEIHHRYSEFASLRVQLSRLYPTCLVPPLPDKHKIMDY------ 135
Query: 235 SKSSYLLPTESGDSVDLSLSVINGPVTTNDEKLIRHRIRMLTSFLNRLLKNQEITKTSIV 294
+IN +++ R R+L FL R+ ++ + + +
Sbjct: 136 --------------------LINVTKNQRMSRMLEERKRLLQLFLRRVAQHPILGLSEVF 175
Query: 295 YDFLDPNNKNWNDXXXXXXXXXXXPKSVLQCNPIDPTNTTKAHSY--LPVPSSSTQLLAS 352
FL + +W + PK +L+ P DP++ A Y LP+PS + L
Sbjct: 176 RKFLS-RHVSWKE-VLHSPPISCLPKDLLKAPPADPSSKENAELYKELPIPSKT---LVP 230
Query: 353 KDNHSASDADEFTKIEAEFKNYEQLIHSGLYKYSRATTKEVNYLREDLKGISSEFAQLST 412
+DN+ F +E + Y + +++ Y + + F LS
Sbjct: 231 RDNYD-DVGKNFLMLEDTLQQYSIVAQEESNLFNQIVLSNSKYCLAH-STLGAMFNALSL 288
Query: 413 DETKNESGLAELLSHSSDAYGTLQEILETLVGNLHYNINEPLSECAHMATAVRDLIHYRR 472
E+ E + ++D + L I + N + EPL E + A +R + +R+
Sbjct: 289 SESGKLLTALEKVGQAND-HTCLASI--DFMHNFVIAVIEPLQELSKDAKNMRHIFIFRK 345
Query: 473 LKLIQKDILERTILYKRGQLIKFQQQENDHKQIDNMVKE 511
+K IQ+ ++E + K+ L +++E ++ + E
Sbjct: 346 MKFIQQVMVEELLTRKKSFLHLLERRERHAARLQQAIGE 384
>SPCC16A11.08 [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 534
Score = 72.0 bits (175), Expect = 3e-12
Identities = 115/523 (21%), Positives = 204/523 (38%), Gaps = 78/523 (14%)
Query: 166 QILEASKVSEGQGRTYIAYAIKYGDSIVKRRYSDFESLRKVLVKLFPISLIPPIPEKQSL 225
QI+ S+G +Y+ Y I + V RRYSDFESL K++ + +P +++PPIP KQSL
Sbjct: 28 QIVGTVTSSDG---SYVEYEIVHQKRSVWRRYSDFESLVKLMRRQYPAAIVPPIPGKQSL 84
Query: 226 KSYGKAMTYSKS--------SYLLP---------------------TESGDSVDLSLSVI 256
SY K +KS S +L S S ++LS +
Sbjct: 85 LSYAKHPRKAKSDAEFLNFRSRMLELFLRQCLLHPCIRSNPIFDKFIHSTVSWHVTLSSL 144
Query: 257 NGPVTTNDEKL--------------IRHRIRM-----LTSFLNRLLKNQEITKTSIVYDF 297
+ P +N + L +R + + L+ +R LK
Sbjct: 145 DLPKDSNTDPLRLPPIATEHDPFAHLRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASS 204
Query: 298 LDPNNKNWNDXXXXXXXXXXXPKSVLQCNPIDPTNTTKAH-SYLPVPSSSTQLLASKDNH 356
L+P++ ++ P L +P PT H + P S TQ +S N
Sbjct: 205 LEPSSPLGSEDECHPPTSDMQPTHELNESPSTPTAPDFPHYNSSPSELSPTQRRSSISNG 264
Query: 357 SASDA---DEFTKIEAEFKNYEQLIHSGLYKYSRATTKEVNYLREDLKGISSEFAQLSTD 413
+ + + T E + +H L +T ++ + L + S F L+
Sbjct: 265 KDAPSPVLKDLTSYTQEVIVCRKFLHHSLSPSIHSTLSSISKMESCLSKLGSAFHSLT-- 322
Query: 414 ETKNESGLAELLSHSSDAYGTLQEILETLVGNLHYNINEPLSECAHMATAVRDLIHYRRL 473
NE LA L ++A+ + + ++ E + + +A D + Y++L
Sbjct: 323 -ALNEIQLANHLQVIANAFEFSGMYAKEFEQEFNSSVYEKMVQSMQLAKCAADALKYKQL 381
Query: 474 KLIQKDILERTILYKRGQLIKFQQQENDHKQIDNMVKEELGTNGAVNLEN-PAGPQSYSG 532
K+ Q+D L+ +++ N D+MV + A L S +
Sbjct: 382 KIQQRDFLQDQLIH-----------SNTMSATDSMVAASPTIHPATRLNTIQRAVVSQAK 430
Query: 533 KFINKFTQLAIIIKDSVSYQEQDPAAAARSLEKELIQLDETLKVATSDLVVISETLKNTE 592
K F +L ++ D V E + + + SL++ ++ L A D I E + + E
Sbjct: 431 KGYTIFGRLQNVLHDFV---EGETSISKESLQQHKNTIENQLAAANWDCQKIDEFM-DAE 486
Query: 593 LPNFIKERDEELTQIFKNYAKYMKENATRNLEIW----KELHN 631
L + + + + +I K+ + + + A NL+ W +EL N
Sbjct: 487 LKFYKECQTSQWEEIIKSVHECIYKWAQTNLQRWLRTREELEN 529
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.310 0.129 0.351
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,053,540
Number of Sequences: 60738
Number of extensions: 1508400
Number of successful extensions: 4650
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4633
Number of HSP's gapped (non-prelim): 10
length of query: 636
length of database: 30,389,216
effective HSP length: 113
effective length of query: 523
effective length of database: 23,525,822
effective search space: 12304004906
effective search space used: 12304004906
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)