ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2360 good A KOG0839 RNA processing and modification RNA Methylase, SpoU family
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2360 820095 824285 1397
(1397 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL112w [A] KOG0839 RNA Methylase SpoU family 950 0.0
Hs19743836 [A] KOG0839 RNA Methylase SpoU family 156 2e-37
At4g17610 [A] KOG0839 RNA Methylase SpoU family 138 5e-32
CE02044 [A] KOG0839 RNA Methylase SpoU family 125 6e-28
>YDL112w [A] KOG0839 RNA Methylase SpoU family
Length = 1436
Score = 950 bits (2456), Expect = 0.0
Identities = 545/1304 (41%), Positives = 799/1304 (60%), Gaps = 53/1304 (4%)
Query: 134 NSLDDGLILDGTKLLDILEVLVQGSASGSYNDLIANSVNIGKLLFL---LSLSKDEKLSN 190
N + L LD LL LEV+ D N ++ L F+ L E++++
Sbjct: 146 NIKHENLHLDILSLLHYLEVVY-------LFDECKNGISSKCLDFIIVPLLGCNSEEIAD 198
Query: 191 AATSLFKWWIPSFATACKEDEKLDITVWSLLRNLLTE-SAPITVAKNGYIFCLRMLMEDG 249
+ + L +W I + C D D +W+ ++ L E S KN F LR L+
Sbjct: 199 SCSKLMRWHIKYLSKCCNTDSNFDKLIWTFIKQLYAEGSQQAWKQKNSLSFLLRFLLAAE 258
Query: 250 FSTTSQSFAQSKDYWNLIQSGLNQEVHEIKKIVLSIMKLSLQLLAQNNLPILTDIIQFS- 308
S ++ ++ YW IQ+ L+ +VHE +K+ LSI+KL++Q L+ + + + T + +
Sbjct: 259 LSPELITYIKTDAYWRHIQTELDNDVHEHRKLALSILKLTIQKLSSHGITLQTTFYKCND 318
Query: 309 -GSRSEIESWKKFTTLYEIVGIDTALNQCEAAAPDILDIFGDPNVHASWGLIILSTGLRA 367
+ + SWKKFTTLYE++ +DT+LNQ +AA DI+ IF + ++H SWGLI+LSTGL++
Sbjct: 319 LANIEMLGSWKKFTTLYEMIALDTSLNQIQAAKQDIIKIFDNEHLHHSWGLILLSTGLKS 378
Query: 368 TMESVRKFTLRLMLQIKHKAVFNANTRLLCDVFLPATMQASFFNVADEHCEYGESVANFV 427
+MESVRK+ + LM I + + F++N LL LPA M A +F+V C +GE ++ FV
Sbjct: 379 SMESVRKYMMTLMFSITNMSAFSSNLPLLTKTLLPAAMSAHYFDVKGVSCPHGEKLSLFV 438
Query: 428 AEILL-TSDDKGTILELLLNALYDFRNMFDAGRIYIAYGVLCGVEKLKERVFETRHIEII 486
+L T++ IL +L L + FD RIY++YG+L + K++ + H+ +I
Sbjct: 439 NNLLSQTTEGISDILFEILKLLVEKGTSFDPSRIYLSYGILVFFQNNKQKTINSDHLSLI 498
Query: 487 RMLFLCRCEENILQTTIQSIYLKYMLF--SNLSAPEFLQTLAMHVKSDNG-YEYISRTIG 543
R L+ EE +L+TTIQ+IYLK++L+ ++SA E L TL H+K G Y+Y+
Sbjct: 499 RKLYDFAAEEEVLETTIQTIYLKFLLYIDPSVSASELLFTLVSHIKLKGGTYKYVEPLFE 558
Query: 544 SFRDFCIVNYN--QSLEGY--EVPQDALYQVLAYELFDCEPYEISDQFLIEIVKADCNYS 599
+RD + +++ Q+ E + +D ++ +LA +FD + +I+ FLIE+ K+ +
Sbjct: 559 DYRDLAVSHFDDLQAKENLTTNIGKDTIFDLLASIIFDFKDIDITPDFLIEVAKSKQDIP 618
Query: 600 DLSEEYYAFLTELVTMESNN---YKEAKVILDLPQFTPAVYGAINPLPIFKSLFSEFLPD 656
+ + FLT+L++ E +N Y+ A +L P FT + + +IN +FKS+ +F D
Sbjct: 619 VYTSKAVTFLTQLLSGEPSNGYTYENATALLSYPNFTISTWKSINVNNLFKSVMEKFSLD 678
Query: 657 KFDFFVSLFDKVMQSP-SQITISFENLLNLYRIIGEYIRSENNLPFKLKDEIYANFFKLL 715
KF FF ++ K + I ++F +LL+LY ++ + + FK+KD Y+++F+LL
Sbjct: 679 KFKFFAEIYQKTYECRFDTIELNFNDLLSLYEMVKKSANQCSRESFKVKDSAYSSYFELL 738
Query: 716 RICIK-------SSPLTEEQLNTLLDLLQENAERDNGNYLGNLEISYLCQSILDVYILPT 768
+K SS +++L+ LL L+ EN +DNGNYLGNL + L I+D YI +
Sbjct: 739 NTFLKTYALNRDSSEGNDDELHILLRLVDENINKDNGNYLGNLAVCKLLYFIIDSYIHCS 798
Query: 769 LHELTDMEKHVLNSAMGLQCRIWDSLIEERLVLNQQPLHLALIRGIFHYTTFLSALND-- 826
++D + ++ IW+ + ERLVL ++ LHL LI+G+FH N
Sbjct: 799 T-SVSDDDIFIVKFIFEKFSFIWECINSERLVLKERDLHLMLIKGLFHPVILYFGSNQYI 857
Query: 827 QAIFKIMLHYATAILDQSFSRRTLKPVLSQQILGFMEVYGSSLPNQ-QYSDFARLLSDVF 885
+ + +A I+ S+SRR+L P+L Q+ FM+ YG L Y ++ VF
Sbjct: 858 DTLTSKLEEHAQTIISLSYSRRSLLPLLGSQLRVFMKFYGKLLREDVNYWWLINIIVGVF 917
Query: 886 THESMNLNIYNLKPAIAKLFDSKLGLYIDGG--LYKFVYGATEVVTKSRIIKALLYSSKE 943
M++N+Y LKP I+ LFD KL Y G LY+ VYG E++ + II ++LY++ +
Sbjct: 918 KQPQMDVNLYKLKPVISSLFDHKLNNYYIKGDELYEKVYGPDEILARVSIIDSILYANDQ 977
Query: 944 FRRQFLQVMLQEDPNMLKAKKKTDGPEEIQRLQKWQLCLLSIKSVDKDVLTGLCSDYIIP 1003
+ + ++ + E N L A K+TDG E +QRL +WQL LLS+ + ++ L+ I+
Sbjct: 978 LKIRLIE-KVTEKTNALYAIKRTDGAEALQRLLQWQLLLLSLLTTNEKKLSETSMIRILK 1036
Query: 1004 SLVEESSPLVRVYSEWFIAYDLWVTATDNHKNPN----EDFLFKLLEDTSRPVLQASVER 1059
S+ +ESSPLVRVY EWFI+ + D +K N ED+LF LLED S+PV S E+
Sbjct: 1037 SIEDESSPLVRVYKEWFISSKV----VDYYKTGNPKFAEDYLFSLLEDHSKPVFVVSAEK 1092
Query: 1060 LLFLSLKAAKETGH---FFRPMERFVAQLITNCSSNKPLIRHFSNSLILLFWPTFEDDIK 1116
+ F+ LK + F + ++RF+ L+ N +SNKPL+RHFSNSLI+ WPTF+ +
Sbjct: 1093 ICFMVLKDLRNDEKKYGFTQLLDRFICTLVPNAASNKPLVRHFSNSLIISLWPTFKAYLS 1152
Query: 1117 DNILRSIISKLYSDAKKIQVQGQFRAGDANTWSINNDLKLTSIFGGVLLKIMDHKVPYIN 1176
D+ LR+II LYS+AKK Q+ GQ+RAGDAN W + D KLT++FGGVL K+ DH PYI+
Sbjct: 1153 DHTLRNIIENLYSNAKKTQIFGQYRAGDANIWDLKGDRKLTNMFGGVLKKVTDHDCPYIS 1212
Query: 1177 SKIFKAINIDDGEFEIGSEESELWLTKRSSNL--VNSTELA-KNSPLQTKSGAWEAVLDF 1233
+F+ + IG++E LWL KR +N VN+ ++ SPLQTKSGAWE VLD
Sbjct: 1213 ESVFEKYLQEKDIVPIGTDERSLWLDKRDTNTESVNNANISCDTSPLQTKSGAWETVLDL 1272
Query: 1234 DNQKSIESVARSELIVVASLVDKAPNLGGICRLCDVLGVGTMTIHDIRVKQHPQFKNVAV 1293
DN+KS + V RSELIVV+SLVDK PNLGGICRLCDVLGVG +T+ DI+VK HPQFKNVAV
Sbjct: 1273 DNKKSNDVVTRSELIVVSSLVDKPPNLGGICRLCDVLGVGLLTVQDIKVKNHPQFKNVAV 1332
Query: 1294 TADRWMPIEEVPVDGIISYMNAKKLEGYTLIGLEQTDSSIQLNSEFKFPSKSVILLGTEA 1353
TADRWMP+EEV +D I S+M KK EGYTLIGLEQTD S++L++ F+FP KS+ILLGTEA
Sbjct: 1333 TADRWMPMEEVALDEIASFMKEKKKEGYTLIGLEQTDKSVKLDNNFQFPKKSLILLGTEA 1392
Query: 1354 HGIPGHLLKELDLCLEIKQSGVIRSMNIQTATAVIVHSYSIQHL 1397
GIPG LL ELDLCLEI+Q GVIRSMNIQTATAVIVHSY++QH+
Sbjct: 1393 FGIPGTLLSELDLCLEIQQFGVIRSMNIQTATAVIVHSYTVQHM 1436
>Hs19743836 [A] KOG0839 RNA Methylase SpoU family
Length = 1621
Score = 156 bits (394), Expect = 2e-37
Identities = 80/199 (40%), Positives = 128/199 (64%), Gaps = 8/199 (4%)
Query: 1206 SNLVNSTELAKNSPLQTKSGAWEA-VLDFDNQKSIESVAR------SELIVVASLVDKAP 1258
+NLV + A+ + +Q K W + V D D + + A S LIVVASL+DK
Sbjct: 1416 TNLVEADNQAEWTDVQKKIIPWNSRVSDLDLELLFQDRAARLGKSISRLIVVASLIDKPT 1475
Query: 1259 NLGGICRLCDVLGVGTMTIHDIRVKQHPQFKNVAVTADRWMPIEEVPVDGIISYMNAKKL 1318
NLGG+CR C+V G + + ++ QF++++V+A++W+P+ EV +I Y+ KK
Sbjct: 1476 NLGGLCRTCEVFGASVLVVGSLQCISDKQFQHLSVSAEQWLPLVEVKPPQLIDYLQQKKT 1535
Query: 1319 EGYTLIGLEQTDSSIQLNSEFKFPSKSVILLGTEAHGIPGHLLKELDLCLEIKQSGVIRS 1378
EGYT+IG+EQT S+ L +++ FP KS++LLG E GIP +L+++LD+C+EI Q G+IRS
Sbjct: 1536 EGYTIIGVEQTAKSLDL-TQYCFPEKSLLLLGNEREGIPANLIQQLDVCVEIPQQGIIRS 1594
Query: 1379 MNIQTATAVIVHSYSIQHL 1397
+N+ + A+++ Y+ Q L
Sbjct: 1595 LNVHVSGALLIWEYTRQQL 1613
>At4g17610 [A] KOG0839 RNA Methylase SpoU family
Length = 1850
Score = 138 bits (348), Expect = 5e-32
Identities = 64/152 (42%), Positives = 105/152 (68%), Gaps = 1/152 (0%)
Query: 1244 RSELIVVASLVDKAPNLGGICRLCDVLGVGTMTIHDIRVKQHPQFKNVAVTADRWMPIEE 1303
R LI+VASLVD+ PNL G+ R C+V ++ + D + QF+ ++VTA++W+PI E
Sbjct: 1697 RQSLILVASLVDRIPNLAGLARTCEVFKASSLAVADASIIHDKQFQLISVTAEKWVPIME 1756
Query: 1304 VPVDGIISYMNAKKLEGYTLIGLEQTDSSIQLNSEFKFPSKSVILLGTEAHGIPGHLLKE 1363
VPV+ + ++ KK EG++++GLEQT +S+ L+ +++FP K+V++LG E GIP ++
Sbjct: 1757 VPVNSLKLFLEKKKREGFSILGLEQTANSVSLD-KYQFPKKTVLVLGREKEGIPVDIIHI 1815
Query: 1364 LDLCLEIKQSGVIRSMNIQTATAVIVHSYSIQ 1395
LD C+EI Q GV+RS+N+ + A+ + Y+ Q
Sbjct: 1816 LDACIEIPQLGVVRSLNVHVSGAIALWEYTRQ 1847
>CE02044 [A] KOG0839 RNA Methylase SpoU family
Length = 1203
Score = 125 bits (313), Expect = 6e-28
Identities = 61/149 (40%), Positives = 96/149 (63%), Gaps = 1/149 (0%)
Query: 1247 LIVVASLVDKAPNLGGICRLCDVLGVGTMTIHDIRVKQHPQFKNVAVTADRWMPIEEVPV 1306
LIVVASLVDK NLGGICR ++ GV T+ + D+ V Q FK ++++++ W IE V
Sbjct: 1050 LIVVASLVDKPNNLGGICRTSEIFGVDTLVVADVLVAQDANFKALSMSSENWQKIEGVKR 1109
Query: 1307 DGIISYMNAKKLEGYTLIGLEQTDSSIQLNSEFKFPSKSVILLGTEAHGIPGHLLKELDL 1366
++ Y+ + GYT+I EQT S+ ++ +F FP K+VI++G E G+P +LL+ +D
Sbjct: 1110 TNLLPYLQNLRANGYTVIAAEQTTDSVMMH-DFVFPKKAVIVMGDEKEGVPVNLLRAVDQ 1168
Query: 1367 CLEIKQSGVIRSMNIQTATAVIVHSYSIQ 1395
+EIKQ G RS+N+ A+++ ++ Q
Sbjct: 1169 TVEIKQVGHTRSLNVHVTAALMIAKFAEQ 1197
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.137 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,596,605
Number of Sequences: 60738
Number of extensions: 3346499
Number of successful extensions: 8373
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 8346
Number of HSP's gapped (non-prelim): 9
length of query: 1397
length of database: 30,389,216
effective HSP length: 119
effective length of query: 1278
effective length of database: 23,161,394
effective search space: 29600261532
effective search space used: 29600261532
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)