ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2512 normal H KOG2743 Coenzyme transport and metabolism Cobalamin synthesis protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2512 879048 877369 -560
(560 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
At1g80480 [H] KOG2743 Cobalamin synthesis protein 145 2e-34
At1g15730 [H] KOG2743 Cobalamin synthesis protein 145 2e-34
SPBC15D4.05 [H] KOG2743 Cobalamin synthesis protein 125 1e-28
At1g26520 [H] KOG2743 Cobalamin synthesis protein 125 1e-28
Hs20532699 [H] KOG2743 Cobalamin synthesis protein 116 8e-26
Hs13236499 [H] KOG2743 Cobalamin synthesis protein 116 8e-26
Hs20481017 [H] KOG2743 Cobalamin synthesis protein 113 7e-25
YNR029c [H] KOG2743 Cobalamin synthesis protein 105 1e-22
>At1g80480 [H] KOG2743 Cobalamin synthesis protein
Length = 444
Score = 145 bits (366), Expect = 2e-34
Identities = 85/257 (33%), Positives = 142/257 (55%), Gaps = 46/257 (17%)
Query: 18 IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSELNVDAALIKDHKVANKEEKLI 77
IP TI++GFLGSGKTTLL ILT +HG ++AVI N+ E+++D +L+ + E ++
Sbjct: 89 IPATIITGFLGSGKTTLLNHILTRDHGKRIAVIENEFGEVDIDGSLVASKSIG--AEDIV 146
Query: 78 QLQNGCICCTLRGDLLE---ELINLHQNNEFDYILIESTGIAEPMQVAETFSSEFSQTLL 134
L NGC+CCT+RGDL+ EL+N + +FD+I+IE+TG+A P + +TF +E
Sbjct: 147 MLNNGCLCCTVRGDLVRMIGELVNT-KKGKFDHIVIETTGLANPAPIIQTFYAE------ 199
Query: 135 DTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANIETTDFLANRWGDSGQ 194
+ + +D K+D VT++DA + +++
Sbjct: 200 ---EEIFND-----------------VKLDGVVTLVDAKHARLHLDEVK----------- 228
Query: 195 GEADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKIIKTSYCKVPM 254
V + ++QI +AD II+NKTD + + L + + IK++N +A++ +T Y V +
Sbjct: 229 ---PEGVVNEAVEQIAYADRIIVNKTDLVGEAELGSVVQRIKTINSMAQMTRTKYGNVDL 285
Query: 255 SEILNTGLYNFEKAASS 271
+L G ++ E+ SS
Sbjct: 286 DYVLGIGGFDLERIESS 302
>At1g15730 [H] KOG2743 Cobalamin synthesis protein
Length = 448
Score = 145 bits (366), Expect = 2e-34
Identities = 90/274 (32%), Positives = 147/274 (52%), Gaps = 48/274 (17%)
Query: 18 IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSELNVDAALIKDHKVANKEEKLI 77
IP TI++GFLGSGKTTLL ILT +HG ++AVI N+ E+++D +L+ E ++
Sbjct: 92 IPATIITGFLGSGKTTLLNHILTGDHGKRIAVIENEFGEVDIDGSLVAAQTAG--AEDIM 149
Query: 78 QLQNGCICCTLRGDLLEELINLHQNNE--FDYILIESTGIAEPMQVAETFSSEFSQTLLD 135
L NGC+CCT+RGDL+ + + Q + FD+I+IE+TG+A P + +TF +E
Sbjct: 150 MLNNGCLCCTVRGDLVRMISEMVQTKKGRFDHIVIETTGLANPAPIIQTFYAE------- 202
Query: 136 TPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANIETTDFLANRWGDSGQG 195
D + +D K+D VT++DA + +++ +G
Sbjct: 203 --DEIFND-----------------VKLDGVVTLVDAKHARLHLDEV---------KPEG 234
Query: 196 EADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKIIKTSYCKVPMS 255
+ V +QI +AD II+NKTD + + L + + IK++N +A + +T Y KV +
Sbjct: 235 YVNEAV-----EQIAYADRIIVNKTDLVGEPELASVMQRIKTINSMAHMKRTKYGKVDLD 289
Query: 256 EILNTGLYNFEKAASSAGWLKSINEMTLKEGYGD 289
+L G ++ E+ SS + E +EG+ D
Sbjct: 290 YVLGIGGFDLERIESSV----NEEEKEDREGHDD 319
>SPBC15D4.05 [H] KOG2743 Cobalamin synthesis protein
Length = 411
Score = 125 bits (315), Expect = 1e-28
Identities = 87/270 (32%), Positives = 147/270 (54%), Gaps = 48/270 (17%)
Query: 17 PIPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSEL-NVDAALIKDHKVANKEEK 75
P+PVTIL+GFLG+GKT+LL IL + +G +VAV++N++ + +++ +L++D E+
Sbjct: 60 PVPVTILTGFLGAGKTSLLRSILENRNGKRVAVLMNEVGDSGDLERSLMEDVGGEELYEE 119
Query: 76 LIQLQNGCICCTLRGDLLEELIN-LHQNNEFDYILIESTGIAEPMQVAETFSSEFSQTLL 134
+ L NGC+CCT++ + ++ L + Q FD I+IE+TGIA P +A+TF +
Sbjct: 120 WVALSNGCMCCTVKDNGIKALEKIMRQKGRFDNIVIETTGIANPGPLAQTFWLD------ 173
Query: 135 DTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANIETTDFLANRWGDSGQ 194
D++ SD K+D VTVID N + NI +
Sbjct: 174 ---DALKSD-----------------VKLDGIVTVIDCKN-IDNILKDE----------- 201
Query: 195 GEADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKIIKTSYCKV-P 253
+D+ QI AD +I+NKTD I + L+ +++ I +N +AKII+T+Y ++
Sbjct: 202 -------SDIGFIQISHADCLILNKTDLISSEALSVVRQTILKINCLAKIIETTYGRLDD 254
Query: 254 MSEILNTGLYNFEKAASSAGWLKSINEMTL 283
+SEIL+ Y E ++ ++ N+ +
Sbjct: 255 ISEILDLDAYGNENTSNLEWSIQRSNDSNI 284
>At1g26520 [H] KOG2743 Cobalamin synthesis protein
Length = 301
Score = 125 bits (315), Expect = 1e-28
Identities = 85/259 (32%), Positives = 136/259 (51%), Gaps = 46/259 (17%)
Query: 12 KAHSGP----IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDM-SELNVDAALIKD 66
K HS + V++++G+LG+GK+TL+ IL HG ++AVI+N+ E+ V+ A+I +
Sbjct: 32 KVHSSSDTVSVGVSVITGYLGAGKSTLVNYILNGKHGKRIAVILNEFGEEIGVERAMINE 91
Query: 67 HKVANKEEKLIQLQNGCICCTLRGDLLEELINLHQNNE-FDYILIESTGIAEPMQVAETF 125
+ E+ ++L NGC+CCT++ L++ L L Q + D+IL+E+TG+A P +A
Sbjct: 92 GEEGAIVEEWVELANGCVCCTVKHSLVQALEQLVQRKDRLDHILLETTGLANPAPLA--- 148
Query: 126 SSEFSQTLLDTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANIETTDFL 185
++L D LE K+D VTV+DA N L
Sbjct: 149 ------SILWLDDQ-----------------LESEVKLDCIVTVVDAKNLRFQ------L 179
Query: 186 ANRWGDSGQGEADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKII 245
R S EA +QI FAD II+NK D I ++ +++K I S+N +A +I
Sbjct: 180 NERRDSSSFPEA--------FNQIAFADTIIMNKVDLISQEESDELEKEIHSINSLANVI 231
Query: 246 KTSYCKVPMSEILNTGLYN 264
++ C+V +S ILN Y+
Sbjct: 232 RSVRCQVDLSNILNCQAYD 250
>Hs20532699 [H] KOG2743 Cobalamin synthesis protein
Length = 395
Score = 116 bits (291), Expect = 8e-26
Identities = 77/245 (31%), Positives = 128/245 (51%), Gaps = 45/245 (18%)
Query: 18 IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSELNVDAALIKDHKVANKEEKLI 77
IPVTI++G+LG+GKTTLL ILT H +VAVI+N+ E + + + E+ +
Sbjct: 42 IPVTIITGYLGAGKTTLLNYILTEQHSKRVAVILNEFGEGSALEKSLAVSQGGELYEEWL 101
Query: 78 QLQNGCICCTLRGDLLEELINLHQ-NNEFDYILIESTGIAEPMQVAETF--SSEFSQTLL 134
+L+NGC+CC+++ + L + NL Q +FDYIL+E+TG+A+P VA F +E +
Sbjct: 102 ELRNGCLCCSVKDNGLRAIENLMQKKGKFDYILLETTGLADPGAVASMFWVDAELGSDIY 161
Query: 135 DTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANIETTDFLANRWGDSGQ 194
+D +T++D+ L + LA D
Sbjct: 162 ----------------------------LDGIITIVDSKYGLKH------LAEEKPDGLI 187
Query: 195 GEADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKIIKTSYCKVPM 254
EA R Q+ AD I+INKTD + ++ + K++ I+S+N + +I++T +V +
Sbjct: 188 NEATR--------QVALADAILINKTDLVPEEDVKKLRATIRSINGLGQILETQRSRVDL 239
Query: 255 SEILN 259
S +L+
Sbjct: 240 SNVLD 244
>Hs13236499 [H] KOG2743 Cobalamin synthesis protein
Length = 395
Score = 116 bits (291), Expect = 8e-26
Identities = 78/247 (31%), Positives = 130/247 (52%), Gaps = 49/247 (19%)
Query: 18 IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSELNVDAALIKDHKVANKEEKLI 77
IPVTI++G+LG+GKTTLL ILT H +VAVI+N+ E + + + E+ +
Sbjct: 42 IPVTIITGYLGAGKTTLLNYILTEQHSKRVAVILNEFGEGSALEKSLAVSQGGELYEEWL 101
Query: 78 QLQNGCICCTLRGDLLEELINLHQ-NNEFDYILIESTGIAEPMQVAETF--SSEFSQTLL 134
+L+NGC+CC+++ + L + NL Q +FDYIL+E+TG+A+P VA F +E +
Sbjct: 102 ELRNGCLCCSVKDNGLRAIENLMQKKGKFDYILLETTGLADPGAVASMFWVDAELGSDIY 161
Query: 135 DTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANI--ETTDFLANRWGDS 192
+D +T++D+ L ++ E D L N
Sbjct: 162 ----------------------------LDGIITIVDSKYGLKHLTEEKPDGLIN----- 188
Query: 193 GQGEADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKIIKTSYCKV 252
EA R Q+ AD I+INKTD + ++ + K++ I+S+N + +I++T +V
Sbjct: 189 ---EATR--------QVALADAILINKTDLVPEEDVKKLRTTIRSINGLGQILETQRSRV 237
Query: 253 PMSEILN 259
+S +L+
Sbjct: 238 DLSNVLD 244
>Hs20481017 [H] KOG2743 Cobalamin synthesis protein
Length = 395
Score = 113 bits (283), Expect = 7e-25
Identities = 77/247 (31%), Positives = 130/247 (52%), Gaps = 49/247 (19%)
Query: 18 IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSELNVDAALIKDHKVANKEEKLI 77
IPVTI++G+LG+GKTTLL ILT H +VAVI+N+ E + + + E+ +
Sbjct: 42 IPVTIITGYLGAGKTTLLNYILTEQHSKRVAVILNESGEGSALEKSLAVSQGGELYEEWL 101
Query: 78 QLQNGCICCTLRGDLLEELINLHQ-NNEFDYILIESTGIAEPMQVAETF--SSEFSQTLL 134
+L+NGC+CC+++ + L + NL Q +FD IL+E+TG+A+P VA F +E +
Sbjct: 102 ELRNGCLCCSVKDNGLRAIENLMQKKGKFDDILLETTGLADPGAVASMFWVDAELGSDIY 161
Query: 135 DTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANI--ETTDFLANRWGDS 192
+D +T++D+ L ++ E D L N
Sbjct: 162 ----------------------------LDGIITIVDSKYGLKHLTEEKPDGLIN----- 188
Query: 193 GQGEADRTVTDLMIDQIEFADVIIINKTDRIKKKRLTKIQKIIKSLNLVAKIIKTSYCKV 252
EA R Q+ AD+I+INKTD + ++ + K++ I+S+N + +I++T +V
Sbjct: 189 ---EATR--------QVALADIILINKTDLVPEEDVKKLRTTIRSINGLGQILETQRSRV 237
Query: 253 PMSEILN 259
+S +L+
Sbjct: 238 DLSNVLD 244
>YNR029c [H] KOG2743 Cobalamin synthesis protein
Length = 429
Score = 105 bits (263), Expect = 1e-22
Identities = 71/252 (28%), Positives = 127/252 (50%), Gaps = 35/252 (13%)
Query: 18 IPVTILSGFLGSGKTTLLERILTSNHGMKVAVIINDMSELNVDAALIKDHKVANKEEKLI 77
IPV+I++G+LGSGK+TLLE+I K+AVI+N+ + + + +N ++ +
Sbjct: 71 IPVSIITGYLGSGKSTLLEKIALKGADKKIAVILNEFGDSSEIEKAMTIKNGSNSYQEWL 130
Query: 78 QLQNGCICCTLRG---DLLEELINLHQNNEFDYILIESTGIAEPMQVAETFSSEFSQTLL 134
L NGC+CC+L+ +E+++ + DYIL+E++GIA+P +A+ F
Sbjct: 131 DLGNGCLCCSLKNIGVKAIEDMVE-RSPGKIDYILLETSGIADPAPIAKMF--------- 180
Query: 135 DTPDSVTSDERVLLQKVLDLGGLEKLTKIDTTVTVIDAMNFLANIETTDFLANRWGDSGQ 194
DE GL ID +TV+D + L ++ A+ GD
Sbjct: 181 ------WQDE-----------GLNSSVYIDGIITVLDCEHILKCLDDISIDAHWHGDKVG 223
Query: 195 GEADRTVTDLMIDQIEFADVIIINKTDRIK--KKRLTKIQKIIKSLNLVAKIIKTSYCKV 252
E + T+ Q+ AD II+NK D I+ + + ++++ ++ +N +A + T Y
Sbjct: 224 LEGNLTIAHF---QLAMADRIIMNKYDTIEHSPEMVKQLKERVREINSIAPMFFTKYSDT 280
Query: 253 PMSEILNTGLYN 264
P+ +L+ Y+
Sbjct: 281 PIQNLLDIHAYD 292
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.137 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,362,503
Number of Sequences: 60738
Number of extensions: 1202046
Number of successful extensions: 3621
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3587
Number of HSP's gapped (non-prelim): 16
length of query: 560
length of database: 30,389,216
effective HSP length: 112
effective length of query: 448
effective length of database: 23,586,560
effective search space: 10566778880
effective search space used: 10566778880
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)