ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2533 good O KOG1415 Posttranslational modification, protein turnover, chaperones Ubiquitin C-terminal hydrolase UCHL1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2533 884414 885148 245
(245 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR099w [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 191 6e-49
Hs5174741 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 111 8e-25
7295994 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 98 1e-20
At4g17510 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 84 2e-16
Hs21361091 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 78 1e-14
CE20817 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 70 2e-12
HsM4759284 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 68 1e-11
CE20818 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 66 5e-11
SPAC27F1.03c [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1 57 2e-08
At1g65650 [O] KOG2778 Ubiquitin C-terminal hydrolase 52 1e-06
>YJR099w [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 236
Score = 191 bits (486), Expect = 6e-49
Identities = 103/236 (43%), Positives = 144/236 (60%), Gaps = 10/236 (4%)
Query: 11 RSVVPLESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETI 70
R+VVP+ESNP+VFTNFA+ LGL ++WA DIYSLT+P+LLAF+PRPVKA++LLFP+NE
Sbjct: 6 RAVVPIESNPEVFTNFAHKLGLKNEWAYFDIYSLTEPELLAFLPRPVKAIVLLFPINE-- 63
Query: 71 DSLTDSFKSDVPESKNGSSAPIWFKQNVRNACGXXXXXXXXXXXXXXXTDGSILKQFL-- 128
KS + S IWFKQ+V+NACG GS L FL
Sbjct: 64 -----DRKSSTSQQITSSYDVIWFKQSVKNACGLYAILHSLSNNQSLLEPGSDLDNFLKS 118
Query: 129 TENPASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLY 188
+ +S DD D F++++ + + G + AP A D LH+IT++E++G ++
Sbjct: 119 QSDTSSSKNRFDDVTTDQFVLNVIKENVQTFSTGQSEAPEATADTNLHYITYVEENGGIF 178
Query: 189 ELDGRSKTGPRCLGKISPD-SDLLTEPLVKQRFEWYHGNASESMKLQFSLLGLGPS 243
ELDGR+ +GP LGK P +DL+ + LV+ R Y NA+E L F++LGLGP+
Sbjct: 179 ELDGRNLSGPLYLGKSDPTATDLIEQELVRVRVASYMENANEEDVLNFAMLGLGPN 234
>Hs5174741 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 230
Score = 111 bits (278), Expect = 8e-25
Identities = 65/187 (34%), Positives = 98/187 (51%), Gaps = 14/187 (7%)
Query: 14 VPLESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSL 73
+PLE+NP+V F LGL +W +D+Y + DP+LL+ +PRPV AV+LLFP+ E +
Sbjct: 7 LPLEANPEVTNQFLKQLGLHPNWQFVDVYGM-DPELLSMVPRPVCAVLLLFPITEKYEVF 65
Query: 74 TDSFKSDVPESKNGSSAPIWF-KQNVRNACGXXXXXXXXXXXXXXX--TDGSILKQFLTE 130
+ + ++ ++F KQ + NACG GS LK+FL E
Sbjct: 66 RTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEE 125
Query: 131 N----PASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGL 186
+ P +Y ++ +I + ++ +G T APS +E V+LHFI + DG
Sbjct: 126 SVSMSPEERARYLEN------YDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGH 179
Query: 187 LYELDGR 193
LYELDGR
Sbjct: 180 LYELDGR 186
>7295994 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 227
Score = 97.8 bits (242), Expect = 1e-20
Identities = 65/193 (33%), Positives = 95/193 (48%), Gaps = 17/193 (8%)
Query: 15 PLESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLT 74
PLESNP+V T + + LG+S W++ D+ L D D L +IPRPVKA ILLFP +ET +
Sbjct: 6 PLESNPEVLTKYIHKLGVSPAWSVTDVIGLED-DTLEWIPRPVKAFILLFPCSETYEKHR 64
Query: 75 DSFKSDVPESKNGSSAPIWF-KQNVRNACGXXXXXXXXXXXXXXXTDGSILKQFLTE--- 130
+ E + +++ +Q NACG D +LK FL +
Sbjct: 65 AEEHDRIKEVEEQHPEDLFYMRQFTHNACGTVALIHSVANNKEVDIDRGVLKDFLEKTAS 124
Query: 131 -NPASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLYE 189
+P G+ + D + + +Q+G T A + E+ + HFI + K+G LYE
Sbjct: 125 LSPEERGRALEKDE------KFTADHEALAQEGQTNAANHEKVIH-HFIALVNKEGTLYE 177
Query: 190 LDGRS----KTGP 198
LDGR K GP
Sbjct: 178 LDGRKSFPIKHGP 190
>At4g17510 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 234
Score = 83.6 bits (205), Expect = 2e-16
Identities = 68/213 (31%), Positives = 98/213 (45%), Gaps = 19/213 (8%)
Query: 1 MSQGKKEQKVRSVVPLESNPQVFTNFANSLGLSSDWA-LMDIYSLTDPDLLAFIPRPVKA 59
M+ + + +PLESNP V + LGL+ D A D+Y L D +LL +P+PV A
Sbjct: 1 MATASESSSSKRWLPLESNPDVMNQYLWGLGLAPDEAECNDVYGL-DDELLEMVPKPVLA 59
Query: 60 VILLFPLNETIDSLTDSFKSDVPESKNGSSAPIWFKQNVRNACGX--XXXXXXXXXXXXX 117
V+ L+P+ + + ++ E K S + KQ V NACG
Sbjct: 60 VLFLYPITKKSEEERIEQDKEIKE-KVHSDKVYFMKQTVGNACGTIGLLHAIGNITSEIK 118
Query: 118 XTDGSILKQFLTE----NPASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDV 173
+DGS L +F P ++ ++D+ + S++ I GDT A ED
Sbjct: 119 LSDGSFLDRFFKSTANMTPMERAKFLENDSQIEDAHSVAVI------AGDT---PASEDA 169
Query: 174 ELHFITFIEKDGLLYELDGRSKTGPRCLGKISP 206
+ HFI +G LYELDGR K GP G SP
Sbjct: 170 DTHFICLACVEGELYELDGR-KAGPISHGASSP 201
>Hs21361091 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 223
Score = 78.2 bits (191), Expect = 1e-14
Identities = 56/185 (30%), Positives = 87/185 (46%), Gaps = 17/185 (9%)
Query: 15 PLESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLT 74
P+E NP++ + LG++ W +D+ L + + L +P P A++LLFPL ++
Sbjct: 5 PMEINPEMLNKVLSRLGVAGQWRFVDVLGLEE-ESLGSVPAPACALLLLFPLTAQHENFR 63
Query: 75 DSFKSDVPESKNGSSAP--IWFKQNVRNACGXXXXXXXXXXXXXXX--TDGSILKQFLTE 130
K + E K +P + KQ + N+CG DGS+LKQFL+E
Sbjct: 64 ---KKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSE 120
Query: 131 NPASDGQYSDDDAVDDFLVS--ISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLY 188
+ S +D F + I ++ +Q+G ++ V HFI F DG LY
Sbjct: 121 TE----KMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRV---DDKVNFHFILFNNVDGHLY 173
Query: 189 ELDGR 193
ELDGR
Sbjct: 174 ELDGR 178
>CE20817 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 469
Score = 70.5 bits (171), Expect = 2e-12
Identities = 60/204 (29%), Positives = 97/204 (47%), Gaps = 19/204 (9%)
Query: 16 LESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLTD 75
LESNP+ F + +G+S +D++S D ++L FIP P A+IL FP + +
Sbjct: 11 LESNPETINPFLSKIGVSGV-ECVDVFSFDD-EMLQFIPTPQLALILCFPSSGVREFRAK 68
Query: 76 SFKSDVPESKNGSSAP-IWF---KQNVRNACGXXXXXXXXXXXXXXXT--DGSILKQFLT 129
++ KNG I+F K+ + +ACG +G K F
Sbjct: 69 QYEE---VEKNGKKPDGIFFMNQKKEIGHACGTFSLFHSLANLENRVNLGNGKFSKWFEK 125
Query: 130 ENPASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLYE 189
+G+ SD D ++E + E +++G+T P E V HFIT++ K+G L+E
Sbjct: 126 AKLVGEGERSDLLLAD---TDLAEAHKETAEEGETEHP---EHVAYHFITYVNKNGQLFE 179
Query: 190 LDGRSKTGPRCLGKISPDSDLLTE 213
+D S PR LG + DS ++ +
Sbjct: 180 IDSCSPF-PRPLG-ATTDSTMIRD 201
Score = 69.7 bits (169), Expect = 4e-12
Identities = 57/204 (27%), Positives = 95/204 (45%), Gaps = 19/204 (9%)
Query: 16 LESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLTD 75
LESNP+ F +G++ +D+YS D ++L F+P P A+IL FP E + L+
Sbjct: 254 LESNPETINPFLKKIGITG-LECVDVYSFDD-EMLQFVPTPQLALILCFPSAEAREFLSK 311
Query: 76 SFKSDVPESKNGSSAPIWFKQN----VRNACGXXXXXXXXXXXXXXXT--DGSILKQFLT 129
++ KNG F N + NACG G K +
Sbjct: 312 QYEE---VEKNGEKPEGVFFMNQSEEIGNACGTFALFHSLANLENRVNLGKGKFAKWYEK 368
Query: 130 ENPASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLYE 189
++ + SD + D ++E + E +++G+T + V+ HFIT++ K+G LYE
Sbjct: 369 AKLVNEDERSDLLSGD---TDLAEAHEETAEEGET---EQSDHVDYHFITYVNKNGQLYE 422
Query: 190 LDGRSKTGPRCLGKISPDSDLLTE 213
+D + PR LG + DS ++ +
Sbjct: 423 IDSCAPF-PRPLG-ATTDSTMIKD 444
>HsM4759284 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 212
Score = 68.2 bits (165), Expect = 1e-11
Identities = 52/170 (30%), Positives = 79/170 (45%), Gaps = 17/170 (10%)
Query: 30 LGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLTDSFKSDVPESKNGSS 89
LG++ W +D+ L + + L +P P A++LLFPL ++ K + E K
Sbjct: 9 LGVAGQWRFVDVLGLEE-ESLGSVPAPACALLLLFPLTAQHENFR---KKQIEELKGQEV 64
Query: 90 AP--IWFKQNVRNACGXXXXXXXXXXXXXXX--TDGSILKQFLTENPASDGQYSDDDAVD 145
+P + KQ + N+CG DGS+LKQFL+E + S +D
Sbjct: 65 SPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETE----KMSPEDRAK 120
Query: 146 DFLVS--ISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLYELDGR 193
F + I ++ +Q+G ++ V HFI F DG LYELDGR
Sbjct: 121 CFEKNEAIQAAHDAVAQEGQCRV---DDKVNFHFILFNNVDGHLYELDGR 167
>CE20818 [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 216
Score = 65.9 bits (159), Expect = 5e-11
Identities = 55/210 (26%), Positives = 86/210 (40%), Gaps = 20/210 (9%)
Query: 15 PLESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLT 74
PLESNP V +G+S +D+ D I +P AVIL FP + +D +
Sbjct: 7 PLESNPSVINPMIEKMGVSGV-KTVDVLFFDDES----IGKPQHAVILCFPEYKKVDEI- 60
Query: 75 DSFKSDVPESKNGSSAPIWFKQNVRNACGXXXXXXXXXXXXXXXT--DGSILKQFLTENP 132
K ++K + + KQ + NACG DGS K
Sbjct: 61 --MKPIYEQAKAADDSVFFMKQKISNACGTFALFHSLANLEDRINLGDGSFAKWLAEAKK 118
Query: 133 ASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLYELDG 192
+ SD FL + +E+ ++ + DVE HFI F+ K+G+LYE+D
Sbjct: 119 VGIEERSD------FLANNAELAGIHAAAATDGQTAPSGDVEHHFICFVGKNGILYEIDS 172
Query: 193 R----SKTGPRCLGKISPDSDLLTEPLVKQ 218
R + GP + D+ + L+++
Sbjct: 173 RRPFAREIGPTSDATLVKDAGAACQHLIEK 202
>SPAC27F1.03c [O] KOG1415 Ubiquitin C-terminal hydrolase UCHL1
Length = 222
Score = 57.4 bits (137), Expect = 2e-08
Identities = 58/210 (27%), Positives = 95/210 (44%), Gaps = 32/210 (15%)
Query: 15 PLESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLT 74
PLE+ P+V + +G+ D ++ D++SL + + +IPRPV A++ +FP S T
Sbjct: 4 PLENTPEVLEPYLQKIGVQ-DASVFDLFSLEE--IPEYIPRPVHALLFVFP-----SSGT 55
Query: 75 DSFKSDVPESKNGSSAPIWFKQNVRNACGXXXXXXXXXXXXXXXTDGSILKQFLTENP-- 132
+ S +W+ Q + NACG L++ + EN
Sbjct: 56 KTIYKGSRILPKDSDKVLWYPQTIPNACG--------TIGLLHAVSNGELRRKVNENDFI 107
Query: 133 ASDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAP-----SAEEDVE--LHFITFI---- 181
S + ++ ++++ I + + A P +EEDVE LHFI F+
Sbjct: 108 KSLIRTAEGSSIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKS 167
Query: 182 EKDGLLYELDGRSKTGPRCLGKISPDSDLL 211
+ D YELDGR + GP +I +SDLL
Sbjct: 168 KDDNHFYELDGRQE-GPVQHSEI--ESDLL 194
>At1g65650 [O] KOG2778 Ubiquitin C-terminal hydrolase
Length = 330
Score = 51.6 bits (122), Expect = 1e-06
Identities = 60/232 (25%), Positives = 99/232 (41%), Gaps = 24/232 (10%)
Query: 16 LESNPQVFTNFANSLGLSSDWALMDIYSLTDPDLLAFIPRPVKAVILLFPLNETIDSLTD 75
+ES+P VFT + + + ++YSL D D L + RPV +I LF
Sbjct: 6 IESDPGVFTELIQQMQVKGV-QVEELYSL-DSDSLNNL-RPVYGLIFLFKWQA------- 55
Query: 76 SFKSDVPESKNGSSAPIWFKQNVRNACGXXXXXXXXXXX--XXXXTDGSILKQFLTENPA 133
K + P ++ S + Q + NAC + S LK+F T+N
Sbjct: 56 GEKDERPTIQDQVSNLFFANQVINNACATQAILAILLNSPEVDIGPELSALKEF-TKNFP 114
Query: 134 SDGQYSDDDAVDDFLVSISEIYNENSQQGDTAAPSAEEDVELHFITFIEKDGLLYELDGR 193
SD + + D + + + + ++D HFI++I DG+LYELDG
Sbjct: 115 SDLKGLAINNSDSIRAAHNSFARPEPFVPEEQKAATKDDDVYHFISYIPVDGVLYELDG- 173
Query: 194 SKTGPRCLGKISPDSD-----LLTEPLVKQRFEWYHGNASESMKLQFSLLGL 240
K GP LG D + +P++++R E Y +++F+LL +
Sbjct: 174 LKEGPISLGPCPGDQTGIEWLQMVQPVIQERIERY-----SQSEIRFNLLAV 220
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.134 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,595,331
Number of Sequences: 60738
Number of extensions: 591943
Number of successful extensions: 1117
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1094
Number of HSP's gapped (non-prelim): 11
length of query: 245
length of database: 30,389,216
effective HSP length: 104
effective length of query: 141
effective length of database: 24,072,464
effective search space: 3394217424
effective search space used: 3394217424
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)