ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2546 good M KOG0621 Cell wall/membrane/envelope biogenesis Phospholipid scramblase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2546 889626 888724 -301
(301 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR100c [M] KOG0621 Phospholipid scramblase 367 e-101
SPAC343.06c [M] KOG0621 Phospholipid scramblase 189 3e-48
At2g04940 [M] KOG0621 Phospholipid scramblase 142 8e-34
Hs10863877 [M] KOG0621 Phospholipid scramblase 71 2e-12
Hs9966795 [M] KOG0621 Phospholipid scramblase 67 2e-11
7294832_1 [M] KOG0621 Phospholipid scramblase 62 1e-09
CE07359 [M] KOG0621 Phospholipid scramblase 59 1e-08
CE07360 [M] KOG0621 Phospholipid scramblase 52 1e-06
>YJR100c [M] KOG0621 Phospholipid scramblase
Length = 327
Score = 367 bits (941), Expect = e-101
Identities = 183/269 (68%), Positives = 209/269 (77%), Gaps = 17/269 (6%)
Query: 50 NMNNVTIQQYDPIATTILNEPTIIIERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQER 109
N N+ I+ +DPIATTILNEPT+IIERQIE MNV LGFEQAN+YAIMDV GN+I M ER
Sbjct: 59 NSNSGIIKVHDPIATTILNEPTVIIERQIEFMNVFLGFEQANRYAIMDVNGNKIASMMER 118
Query: 110 DFGFFKAITRQIYRLHRPFTVDVFDNYGNVLMTIERPFSFINSHIKAILPD--------- 160
DF KAI RQ YRLHRPF VDVFDN+GNV+MTI+RPFSFINSHIK I+P
Sbjct: 119 DFSITKAIMRQFYRLHRPFLVDVFDNWGNVIMTIKRPFSFINSHIKTIIPPSAYVDNGSD 178
Query: 161 -------ENRTLVGESVQNWHLWRRRYELFQRECENPSEFTQFGNIDAPFLSFEFPVMDE 213
+ T VGE++QNWHLWRRRYELFQ++ S F QFG IDAPFLSF+FPV D
Sbjct: 179 STHYHDGKEGTTVGETIQNWHLWRRRYELFQKDGVEGSTFDQFGKIDAPFLSFDFPVTDA 238
Query: 214 NNKIIAGCDRNWVGIGRELFTDTGVYIVRFDSQQSFDGVYPQEMLSDKVLNYDQRAVMLS 273
+ KI+A DRNWVG+GRE+FTDTGVY+VRFDSQ+ FD +YP EMLS +VL DQRAV+L+
Sbjct: 239 DGKIMASVDRNWVGLGREMFTDTGVYVVRFDSQRCFDNIYPTEMLSSQVLTLDQRAVLLA 298
Query: 274 NAVSIDFDYFSRHSRYGGGLLSF-GTYDE 301
NAVSIDFDYFSRHSR GG LSF G YDE
Sbjct: 299 NAVSIDFDYFSRHSRQTGGFLSFGGGYDE 327
>SPAC343.06c [M] KOG0621 Phospholipid scramblase
Length = 381
Score = 189 bits (481), Expect = 3e-48
Identities = 100/238 (42%), Positives = 150/238 (63%), Gaps = 18/238 (7%)
Query: 63 ATTILNEPTIIIERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQERDFG-FFKAITRQI 121
A +L++ +I+ERQ+EMMNV LG+EQAN+Y I++ G +GY+ E+ +++RQ
Sbjct: 70 AAPLLSQDVLIVERQLEMMNVFLGYEQANRYVILNQQGQHLGYIAEQGASSILSSLSRQF 129
Query: 122 YRLHRPFTVDVFDNYGNVLMTIERPFSFINSHIKAILPDENR---TLVGESVQNWHLWRR 178
+ HR F DV D+ G +++ + RPFS+INS ++ D ++ TLVGE +Q WHLWRR
Sbjct: 130 FHTHRAFKADVMDSNGQLVLQLNRPFSWINSRLQIHSIDYSKFSSTLVGEVLQKWHLWRR 189
Query: 179 RYELFQRECENPSEFTQFGNIDAPFLSFEFPVMDENNKIIAGCDRNWVGIGRELFTDTGV 238
RYELF + S F QF ID LS+EF + +E ++I+ RN++G+ RE FTDTG
Sbjct: 190 RYELFLAK---RSMFEQFAKIDERVLSWEFLLRNEQDRILGSVSRNFMGLPREFFTDTGN 246
Query: 239 YIVRFDSQQSFDGVYPQEMLSD-----------KVLNYDQRAVMLSNAVSIDFDYFSR 285
Y++RF S + +G + L + ++ ++RAVML +AV+IDFDYFSR
Sbjct: 247 YVLRFTSTSAANGSVNENQLLQAAHGIANDVCARDMSLEERAVMLGSAVTIDFDYFSR 304
>At2g04940 [M] KOG0621 Phospholipid scramblase
Length = 392
Score = 142 bits (357), Expect = 8e-34
Identities = 83/240 (34%), Positives = 130/240 (53%), Gaps = 21/240 (8%)
Query: 65 TILNEPTIIIERQIEMMNVVLGFEQANKYAIMDVMGNR--IGYMQERDFGFFKAITRQIY 122
T+L +++ R IE N+VLGFEQ N+Y ++DV +G ++E+ + RQ+
Sbjct: 166 TLLARSNLLVTRDIEWANLVLGFEQENRYIVVDVCYPEAPVGSIREQS----NLLARQLL 221
Query: 123 RLHRPFTVDVFDNYGNVLMTIERPFSFINSHIKAILPDENRTLVGESVQNWHLWRRRYEL 182
R RPF + D GN L + RPF ++ S I A + E +G + WHLWRR Y+L
Sbjct: 222 RTRRPFVASITDALGNELFRVRRPFWWLTSSIYAEIDGEE---IGVVHRRWHLWRRIYDL 278
Query: 183 FQRECENPSEFTQFGNIDAP-FLSFEFPVMDENNKIIAGCDRNWVGIGRELFTDTGVYIV 241
+ QF ++ P F ++ F V D + +++A DR+W G G E+FTD G Y++
Sbjct: 279 YLGN-------NQFAVVENPGFWNWTFTVKDADGEVLAQIDRDWRGFGFEIFTDAGQYVI 331
Query: 242 RFDSQQSFDGVYP----QEMLSDKVLNYDQRAVMLSNAVSIDFDYFSRHSRYGGGLLSFG 297
RF + P +E+ + L +RAV+L+ A+S+D DYFSRH +G ++ G
Sbjct: 332 RFGKADAAAKTGPATLVEELEVKRPLTLSERAVVLTLAISLDNDYFSRHGGWGIPFMAVG 391
>Hs10863877 [M] KOG0621 Phospholipid scramblase
Length = 318
Score = 71.2 bits (173), Expect = 2e-12
Identities = 58/224 (25%), Positives = 96/224 (41%), Gaps = 38/224 (16%)
Query: 72 IIIERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQERDFGFFKAITRQIYRLHRPFTVD 131
I+I +QIE++ V+ GFE NKY I + G R+ + E TR RPFT+
Sbjct: 108 ILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDT----DCCTRNCCGPSRPFTLR 163
Query: 132 VFDNYGNVLMTIERPFSFINSHIKAILPD-----ENRTLVGESVQNWHLWRRRYELFQRE 186
+ DN G ++T+ERP + L + +G +Q WH ++ + +
Sbjct: 164 IIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEK 223
Query: 187 CENPSEFTQ-------FGNIDAPFLSFEFPVMDENNKIIAGCDRNWVGIGRELFTDTGVY 239
E+ + + G++D FE +DE ++ ++W GI RE FTD +
Sbjct: 224 REDVLKISGPCVVCSCCGDVD-----FEIKSLDE-QCVVGKISKHWTGILREAFTDADNF 277
Query: 240 IVRFDSQQSFDGVYPQEMLSDKVLNYDQRAVMLSNAVSIDFDYF 283
++F L+ +AVM+ IDF +F
Sbjct: 278 GIQFPLD----------------LDVKMKAVMIGACFLIDFMFF 305
>Hs9966795 [M] KOG0621 Phospholipid scramblase
Length = 224
Score = 67.4 bits (163), Expect = 2e-11
Identities = 56/220 (25%), Positives = 96/220 (43%), Gaps = 26/220 (11%)
Query: 72 IIIERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQERDFGFFKAITRQIYRLHRPFTVD 131
I+I +QIE++ V+ FE +N Y I + G RI Y D F R RPFT+
Sbjct: 23 ILIHQQIELLEVLFSFESSNMYEIKNSFGQRI-YFAAEDTNF---CIRNCCGRSRPFTLR 78
Query: 132 VFDNYGNVLMTIERPFSFINSHIKAILPD-----ENRTLVGESVQNWHLWRRRYELFQRE 186
+ DN G ++T+ERP L + VG Q WH ++ + ++
Sbjct: 79 ITDNVGREVITLERPLRCNCCCCPCCLQEIEIQAPPGVPVGYVTQTWHPCLTKFTIKNQK 138
Query: 187 CENPSEFTQFGNIDAPFLSFEFPVMDENNKIIAG-CDRNWVGIGRELFTDTGVYIVRFDS 245
E+ + + + + +F + + +I+ G ++W G RE FTD + ++F
Sbjct: 139 REDVLKISGPCIVCSCIAGVDFEITSLDEQIVVGRISKHWSGFLREAFTDADNFGIQF-- 196
Query: 246 QQSFDGVYPQEMLSDKVLNYDQRAVMLSNAVSIDFDYFSR 285
P++ L+ +AVM+ ID+ +F R
Sbjct: 197 --------PRD------LDVKMKAVMIGACFLIDYMFFER 222
>7294832_1 [M] KOG0621 Phospholipid scramblase
Length = 560
Score = 62.0 bits (149), Expect = 1e-09
Identities = 43/185 (23%), Positives = 80/185 (43%), Gaps = 25/185 (13%)
Query: 72 IIIERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQERDFGFFKAITRQIYRLHRPFTVD 131
+++++++E++ GFE NK+ I + +G ++ + E + TR RPF +
Sbjct: 369 LLVKQKVELLEAFTGFETNNKFTIKNALGQKVYFAAEDN----DCCTRNCCGPARPFDMR 424
Query: 132 VFDNYGNVLMTIERP-------FSFINSHIKAILPDENRTLVGESVQNWHLWRRRYELFQ 184
VFDN+ ++ + RP F I+ P N ++G Q W + + +
Sbjct: 425 VFDNFQQEVIHMHRPLACSSCLFPCCLQSIEVSAPPGN--VIGTIEQEWSICSPSFRILN 482
Query: 185 RECENPSEFTQFGNIDAPFLSF------EFPVMDENNKIIAGCDRNWVGIGRELFTDTGV 238
+ I+ PF +F EF V+ + I + W G+ RE+FTD
Sbjct: 483 HVGDTVM------RIEGPFCTFSLCGDVEFNVVSLTGEKIGKISKQWSGLAREIFTDADF 536
Query: 239 YIVRF 243
+ + F
Sbjct: 537 FGINF 541
>CE07359 [M] KOG0621 Phospholipid scramblase
Length = 301
Score = 58.5 bits (140), Expect = 1e-08
Identities = 72/302 (23%), Positives = 127/302 (41%), Gaps = 59/302 (19%)
Query: 15 SQPAALVRQYSAFRRLPRV-ATPVKDVSRLGFNGVPNMNNVTIQQYDPIATTILNEPTII 73
++ A R+ +A +LP +TP+K +++ V V + + I + I + +++
Sbjct: 36 AEGAGFGRRNNAGTKLPAARSTPMKIPNQVAAMPVQMTGFVQLASNN-ILSAIAHTNSLM 94
Query: 74 IERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQERDFGFFKAITRQIYRLHRPFTVDVF 133
+ + IE + + GFE N+Y + D+ + Y ER F RQ + +R F + +
Sbjct: 95 VVQCIEPLEIFTGFETPNRYVVHDMYCRPLLYCMERSNVF----ARQYHGNNRNFGMQIM 150
Query: 134 DNYGNVLMTI--ERPFSFINSHIKAILPDENRTLVGESVQNWHLWRRRYELFQRECENPS 191
D +G +MT RP + + D+ +G + +REC NP+
Sbjct: 151 DTHGAQVMTCFRGRPCCSCDDFLSTQFLDQQ---IG--------------MMKRECCNPN 193
Query: 192 EFTQFGNIDAPFL-----------SFEFPVMDENNKIIAGCDRNWVGIGRELFTDTGVYI 240
+ P L + +FPVM N ++ R + G +E+FTD YI
Sbjct: 194 FELIGSGCNQPLLIQSPVCAGCGGTQKFPVMTYNGALVGEIVRLYPGFMQEMFTDADTYI 253
Query: 241 VRFDSQQSFDGVYPQEMLSDKVLNYDQRAVMLSNAVSIDFDYF-----SRHSRYG--GGL 293
V F P +M +L + +++++ IDF YF RH G GG+
Sbjct: 254 VHF----------PMDM--PPIL----KLLLVTSVFLIDFTYFEDRNQDRHHHGGMHGGM 297
Query: 294 LS 295
S
Sbjct: 298 HS 299
>CE07360 [M] KOG0621 Phospholipid scramblase
Length = 303
Score = 51.6 bits (122), Expect = 1e-06
Identities = 53/224 (23%), Positives = 92/224 (40%), Gaps = 28/224 (12%)
Query: 62 IATTILNEPTIIIERQIEMMNVVLGFEQANKYAIMDVMGNRIGYMQERDFGFFKAITRQI 121
I + I + ++++ + I+ + + GFE N+Y + D+ + Y ER F RQ
Sbjct: 85 ILSAIAHTNSVMVVQCIKPLEIFTGFETPNRYVVHDMYCRPLLYCMERSNIF----ARQY 140
Query: 122 YRLHRPFTVDVFDNYGNVLMTI--ERPFSFINSHIKAILPDENRTLVGESVQNWHLWRRR 179
R F + + D +G +MT RP + + D+ ++ + N
Sbjct: 141 EGNDRNFGMQIMDTHGAQVMTCFRGRPCCSTDDFLSTQFLDQQIGMMKKECCN-----PN 195
Query: 180 YELFQRECENPSEFTQFGNIDAPFLSFEFPVMDENNKIIAGCDRNWVGIGRELFTDTGVY 239
+EL C P G +FPVM N ++ R + G +E+FTD Y
Sbjct: 196 FELVGSGCNQPLLIQSPGCAGCGGTQ-KFPVMTFNGVLVGEIVRLYPGFMQEMFTDADTY 254
Query: 240 IVRFDSQQSFDGVYPQEMLSDKVLNYDQRAVMLSNAVSIDFDYF 283
IV F P +M +L + +++++ IDF YF
Sbjct: 255 IVHF----------PMDM--PPIL----KLLLVTSVFLIDFTYF 282
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.141 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,855,472
Number of Sequences: 60738
Number of extensions: 755918
Number of successful extensions: 1975
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1956
Number of HSP's gapped (non-prelim): 8
length of query: 301
length of database: 30,389,216
effective HSP length: 106
effective length of query: 195
effective length of database: 23,950,988
effective search space: 4670442660
effective search space used: 4670442660
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)