ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2613 good L KOG1918 Replication, recombination and repair 3-methyladenine DNA glycosidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2613 912583 913410 276
(276 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER142c [L] KOG1918 3-methyladenine DNA glycosidase 241 6e-64
At3g50880 [L] KOG1918 3-methyladenine DNA glycosidase 64 2e-10
At1g75230 [L] KOG1918 3-methyladenine DNA glycosidase 63 5e-10
At1g19480 [L] KOG1918 3-methyladenine DNA glycosidase 62 7e-10
SPAPB24D3.04c [L] KOG1918 3-methyladenine DNA glycosidase 61 2e-09
>YER142c [L] KOG1918 3-methyladenine DNA glycosidase
Length = 296
Score = 241 bits (616), Expect = 6e-64
Identities = 116/267 (43%), Positives = 180/267 (66%), Gaps = 1/267 (0%)
Query: 9 LKVDVDQEYLDQHIDSFRDGIEHILTIDPTLKEIIEHSPFTMFLKENVAKDSMDLYFNKL 68
L+V + ++Y+ +H + F EHIL DP+L I++++ FT++LKE ++++ YF +L
Sbjct: 28 LEVALPEKYIARHEEKFNMACEHILEKDPSLFPILKNNEFTLYLKETQVPNTLEDYFIRL 87
Query: 69 AGSIISQQISGAAAKNIRGRIVDMFDGTFPNYQTLHETFQDSKKKQAFKECGLSQRKLMY 128
A +I+SQQISG AA++I+ R+V ++ G FP+Y+ L E F+D K +CGLS+RK++Y
Sbjct: 88 ASTILSQQISGQAAESIKARVVSLYGGAFPDYKILFEDFKDPAKCAEIAKCGLSKRKMIY 147
Query: 129 IESLVGYFFNNEDSIKDLFAFAD-DDKIIEELVANIKGIGPWSAKMFLVTGLHRLNVFAA 187
+ESL YF I+ LF D D+++IE LV N+KGIGPWSAKMFL++GL R++VFA
Sbjct: 148 LESLAVYFTEKYKDIEKLFGQKDNDEEVIESLVTNVKGIGPWSAKMFLISGLKRMDVFAP 207
Query: 188 EDLGIARGCSRYLEQRPEILKKLHXXXXXXXXXXXXXXXXXXXXHDEDVVELCGDLFSPH 247
EDLGIARG S+YL +PE+ K+L +D+D++E C + FSP+
Sbjct: 208 EDLGIARGFSKYLSDKPELEKELMRERKVVKKSKIKHKKYNWKIYDDDIMEKCSETFSPY 267
Query: 248 RTLFMFVLWRMSDTNMEVVINNEKRFM 274
R++FMF+LWR++ TN + ++ E+ F+
Sbjct: 268 RSVFMFILWRLASTNTDAMMKAEENFV 294
>At3g50880 [L] KOG1918 3-methyladenine DNA glycosidase
Length = 273
Score = 63.9 bits (154), Expect = 2e-10
Identities = 45/137 (32%), Positives = 71/137 (50%), Gaps = 5/137 (3%)
Query: 59 DSMDLYFNKLAGSIISQQISGAAAKNIRGRIVDMFDGTFPNYQTLHETFQDSKKKQAFKE 118
DS + F LA SI+ QQ++ AAK I R + +F+G + E+ S ++
Sbjct: 98 DSSNTPFLSLARSILYQQLATKAAKCIYDRFISLFNGGEAG--VVPESVI-SLSAVDLRK 154
Query: 119 CGLSQRKLMYIESLVGYFFNNEDSIKDLFAFADDDKIIEELVANIKGIGPWSAKMFLVTG 178
G+S RK Y+ L + NN +L D+++I+ L +KGIG W+ MF++
Sbjct: 155 IGVSGRKASYLHDLADKY-NNGVLSDELILKMSDEELIDRLTL-VKGIGVWTVHMFMIFS 212
Query: 179 LHRLNVFAAEDLGIARG 195
LHR +V DLG+ +G
Sbjct: 213 LHRPDVLPVGDLGVRKG 229
>At1g75230 [L] KOG1918 3-methyladenine DNA glycosidase
Length = 428
Score = 62.8 bits (151), Expect = 5e-10
Identities = 45/167 (26%), Positives = 76/167 (44%), Gaps = 13/167 (7%)
Query: 29 IEHILTIDPTLKEIIEHSPFTMFLKENVAKDSMDLYFNKLAGSIISQQISGAAAKNIRGR 88
+ H+ ++DP L +I+ P F ++ F L SI+ QQ++ A +I R
Sbjct: 139 LHHLRSVDPLLASLIDIHPPPTF-------ETFQTPFLALIRSILYQQLAAKAGNSIYTR 191
Query: 89 IVDMFDGTFPNYQTLHETFQDSKKKQAFKECGLSQRKLMYIESLVGYFFNNEDSIKDLFA 148
V + G + E +Q ++ G+S RK Y+ L + N + D
Sbjct: 192 FVALCGG---ENGVVPENVLPLTPQQ-LRQIGVSGRKASYLHDLARKYQNG--ILSDSGI 245
Query: 149 FADDDKIIEELVANIKGIGPWSAKMFLVTGLHRLNVFAAEDLGIARG 195
D+K + ++ + GIG WS MF++ LHR +V DLG+ +G
Sbjct: 246 VNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKG 292
>At1g19480 [L] KOG1918 3-methyladenine DNA glycosidase
Length = 1226
Score = 62.4 bits (150), Expect = 7e-10
Identities = 47/167 (28%), Positives = 75/167 (44%), Gaps = 13/167 (7%)
Query: 29 IEHILTIDPTLKEIIEHSPFTMFLKENVAKDSMDLYFNKLAGSIISQQISGAAAKNIRGR 88
I ++ DP L +I+ P F +S F L +I+ QQ++ A +I R
Sbjct: 143 IHYLRNADPLLAALIDVHPPPTF-------ESFKTPFLALIRNILYQQLAMKAGNSIYTR 195
Query: 89 IVDMFDGTFPNYQTLHETFQDSKKKQAFKECGLSQRKLMYIESLVGYFFNNEDSIKDLFA 148
V + G + ET S Q ++ G+S RK Y+ L + N + D
Sbjct: 196 FVSLCGG---ENLVVPETVL-SLNPQQLRQIGVSGRKASYLHDLARKYQNG--ILSDSAI 249
Query: 149 FADDDKIIEELVANIKGIGPWSAKMFLVTGLHRLNVFAAEDLGIARG 195
D+K + ++ + GIG WS MF++ LHR +V DLG+ +G
Sbjct: 250 LNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKG 296
>SPAPB24D3.04c [L] KOG1918 3-methyladenine DNA glycosidase
Length = 228
Score = 60.8 bits (146), Expect = 2e-09
Identities = 51/200 (25%), Positives = 95/200 (47%), Gaps = 12/200 (6%)
Query: 13 VDQEYLDQHIDSFRDGIEHILTIDPTLKEIIEHSPFTMFLKENVAKDSMDLYFNKLAGSI 72
+D E ++ + S H+ +D K +++ + N + + + Y +L ++
Sbjct: 3 LDIEEKEEIVTSLTKAEIHLSGLDENWKRLVK---LVGNYRPNRSMEKKEPY-EELIRAV 58
Query: 73 ISQQISGAAAKNIRGRIVDMFD-GTFPNYQTLHETFQDSKKKQAFKECGLSQRKLMYIES 131
SQQ+ AA I R + + G FP + + + + + CG S RK+ ++S
Sbjct: 59 ASQQLHSKAANAIFNRFKSISNNGQFPTPEEIRDM-----DFEIMRACGFSARKIDSLKS 113
Query: 132 LVGYFFNNEDSIKDLFAFADDDKIIEELVANIKGIGPWSAKMFLVTGLHRLNVFAAEDLG 191
+ + K+ ++++IE L IKGIG W+ +M L+ L+R +V A+DL
Sbjct: 114 IAEATISGLIPTKEEAERLSNEELIERLT-QIKGIGRWTVEMLLIFSLNRDDVMPADDLS 172
Query: 192 IARGCSRYLEQRPEILKKLH 211
I G RYL + P+I K++
Sbjct: 173 IRNG-YRYLHRLPKIPTKMY 191
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.140 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,272,971
Number of Sequences: 60738
Number of extensions: 600721
Number of successful extensions: 1590
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1586
Number of HSP's gapped (non-prelim): 5
length of query: 276
length of database: 30,389,216
effective HSP length: 105
effective length of query: 171
effective length of database: 24,011,726
effective search space: 4106005146
effective search space used: 4106005146
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)