ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2632 good D KOG2864 Cell cycle control, cell division, chromosome partitioning Nuclear division RFT1 protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2632 920415 922082 556
(556 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBL020w [D] KOG2864 Nuclear division RFT1 protein 598 e-171
SPBC887.19 [D] KOG2864 Nuclear division RFT1 protein 202 8e-52
Hs16418361 [D] KOG2864 Nuclear division RFT1 protein 145 2e-34
At5g07630 [D] KOG2864 Nuclear division RFT1 protein 86 1e-16
7290615 [D] KOG2864 Nuclear division RFT1 protein 86 1e-16
CE07621 [D] KOG2864 Nuclear division RFT1 protein 84 7e-16
>YBL020w [D] KOG2864 Nuclear division RFT1 protein
Length = 574
Score = 598 bits (1541), Expect = e-171
Identities = 308/556 (55%), Positives = 395/556 (70%), Gaps = 22/556 (3%)
Query: 8 MNKFANGVLFLMLGQTLSKGVNFLLNTLLVRYLSPRIFGITSFLEFLLSTVLFFSRESIR 67
+ + G FLM+GQ +K V F+LN LL+R+LSPRIFGIT+FLEF+ TVLFFSR++IR
Sbjct: 16 LERSTTGATFLMMGQLFTKLVTFILNNLLIRFLSPRIFGITAFLEFIQGTVLFFSRDAIR 75
Query: 68 ISTLRIKST-----TDSGKLEKVEDGEDTRTLQSLINFGYIPFVIGLPLSIILISWQYSN 122
+STLRI + D + E E ++ LQ+ +NF YIPF IG PLSI LI+WQY N
Sbjct: 76 LSTLRISDSGNGIIDDDDEEEYQETHYKSKVLQTAVNFAYIPFWIGFPLSIGLIAWQYRN 135
Query: 123 LNSYFIDLPYFKASIFLIWLSILIELVSEPFYLVHQYLLNHFIRSKYESLGVTFACVANF 182
+N+YFI LP+F+ SIFLIWLSI++EL+SEPF++V+Q++LN+ RS++ES+ VT C+ NF
Sbjct: 136 INAYFITLPFFRWSIFLIWLSIIVELLSEPFFIVNQFMLNYAARSRFESIAVTTGCIVNF 195
Query: 183 IIVVWFEKMVNGVGLELHDDYKQEGIAIFAFAVGKLVHAMTLLACYSYNYYSEVYTTGER 242
I+V ++ +G+ + D +EGIAI AFA+GKL H++TLLACY ++Y + +
Sbjct: 196 IVVYAVQQSRYPMGV-VTSDIDKEGIAILAFALGKLAHSITLLACYYWDYLKN-FKPKKL 253
Query: 243 YSYKLTKIRPETRQE---------SYYFQNDTVQHFKKVYFQLCFKHLLTEGDKLIINSL 293
+S +LTKI+ E SY+FQND +QHFKKVYFQLCFKHLLTEGDKLIINSL
Sbjct: 254 FSTRLTKIKTRENNELKKGYPKSTSYFFQNDILQHFKKVYFQLCFKHLLTEGDKLIINSL 313
Query: 294 CTVEEQGIYSLLSNYGSLITRLLFAPIXXXXXXXXXXXXSVSSKKNLWLSMKVLIDLTKF 353
CTVEEQGIY+LLSNYGSL+TRLLFAPI S + KNL LS++VL++LT+F
Sbjct: 314 CTVEEQGIYALLSNYGSLLTRLLFAPIEESLRLFLARLLSSHNPKNLKLSIEVLVNLTRF 373
Query: 354 YLYLSLFIIIFGPINSSYLLKFVIGSKWSSTSFLETIRTYCFYIPFLSLNGIFEAFFQSV 413
Y+YLSL II+FGP NSS+LL+F+IGSKWS+TS L+TIR YCFYIPFLSLNGIFEAFFQSV
Sbjct: 374 YIYLSLMIIVFGPANSSFLLQFLIGSKWSTTSVLDTIRVYCFYIPFLSLNGIFEAFFQSV 433
Query: 414 ASGDQIFKHSYVMMLFSGIFLFNCWLFIEYFDLSLEGLIVSNILNMALRIAYCGNFIHKF 473
A+GDQI KHSY MM FSGIFL N WL IE LS+EGLI+SNI+NM LRI YCG F++KF
Sbjct: 434 ATGDQILKHSYFMMAFSGIFLLNSWLLIEKLKLSIEGLILSNIINMVLRILYCGVFLNKF 493
Query: 474 YHYLLKESSTETTQSILPNISTFKNVALIAAIIGALDWYFIGYVENMREXXXXXXXXXXX 533
+ L +S S N FK V + + I LDW+FIGYV+N+++
Sbjct: 494 HRELFTDS------SFFFNFKDFKTVIIAGSTICLLDWWFIGYVKNLQQFVVNVLFAMGL 547
Query: 534 XXXXXYKEKSLISELL 549
KE+ I +
Sbjct: 548 LALILVKERQTIQSFI 563
>SPBC887.19 [D] KOG2864 Nuclear division RFT1 protein
Length = 527
Score = 202 bits (515), Expect = 8e-52
Identities = 143/491 (29%), Positives = 245/491 (49%), Gaps = 44/491 (8%)
Query: 1 MNVKNEHMNKFANGVLFLMLGQTLSKGVNFLLNTLLVRYLSPRIFGITSF-LEFLLSTVL 59
M+ + + + G+ + Q S+ + F LN L +R SP + +S E L ST+L
Sbjct: 1 MDKSDSLLQSSSKGLRSSIFFQGSSRILTFFLNQLTIRLTSPSAYAFSSIHFEILQSTIL 60
Query: 60 FFSRESIRISTLRIKS--------TTDSGKLEKVEDGEDTRTLQSLINFGYIPFVIGLPL 111
F SRES+R++ RI S +T+S K +K+ D LQ + N I IG+ +
Sbjct: 61 FLSRESVRLAMQRIPSENAIITSTSTESNKSKKLSD-----QLQLIKNTSLISVYIGIVI 115
Query: 112 SIILISWQYSNLNSYFIDLPYFKASIFLIWLSILIELVSEPFYLVHQYLLNHFIRSKYES 171
S+++ + + +L ++ PY K IF+ +S IEL+SEP+Y V Q+ + E
Sbjct: 116 SLLVSLFYFYSLPNF----PYSKTCIFIYTVSSFIELLSEPYYEVLQWRQKFSKTASAEG 171
Query: 172 LGVTFACVANFIIVVWFEKMVNGVGLELHDDYKQEGIAIFAFAVGKLVHAMTLLACYSYN 231
LG + +F I V +G + + + FA+G L +T+ ++
Sbjct: 172 LGTIICSLLSFAISV--------LG-------RNKAPSSLPFALGNLSEKVTIF--FTLR 214
Query: 232 YYSEVYTTGERYSYKLTKIRPETRQESYYFQNDTVQHFKKVYFQLCFKHLLTEGDKLIIN 291
Y+++ + +S L K+ R ++ + T++ +Q+ KHL+T+GDK+++
Sbjct: 215 YFAK-----QPFSIFLHKVGENERY--IFWDSSTLRIICSHTYQVLLKHLITKGDKIMVA 267
Query: 292 SLCTVEEQGIYSLLSNYGSLITRLLFAPIXXXXXXXXXXXXSVSSKKNLWLSMKVLIDLT 351
+ QG Y+L SNYGSL+ R++F P+ +KK+ ++ +L +
Sbjct: 268 WYASPSAQGPYALASNYGSLLARIVFRPVEDHSHIVFAQLTHYKNKKDEKKALNLLAWIL 327
Query: 352 KFYLYLSLFIIIFGPINSSYLLKFVIGSKWSSTSFLETIRTYCFYIPFLSLNGIFEAFFQ 411
K Y Y+SLFI+ FG S +L F GSKW+S + Y YIPF++ NG+ EAF+
Sbjct: 328 KLYSYMSLFIL-FGSNYSDIVLLFGAGSKWASPDSSSILSWYAMYIPFMAANGVLEAFYV 386
Query: 412 SVASGDQIFKHSYVMMLFSGIFLFNCWLFIEYFDLSLEGLIVSNILNMALRIAYCGNFI- 470
S A+ Q++ + + + + +F+L GLI++NILN++LRI + FI
Sbjct: 387 SAANSSQLYDQGKCYLASAVFYFITGKFLLSWFNLGSHGLILANILNLSLRICFALRFIL 446
Query: 471 HKFYHYLLKES 481
H + + L S
Sbjct: 447 HNYKDFSLPRS 457
>Hs16418361 [D] KOG2864 Nuclear division RFT1 protein
Length = 541
Score = 145 bits (366), Expect = 2e-34
Identities = 119/485 (24%), Positives = 218/485 (44%), Gaps = 56/485 (11%)
Query: 7 HMNKFANGVLFLMLGQTLSKGVNFLLNTLLVRYLSPRIFGITSF-LEFLLSTVLFFSRES 65
H + A+ L L Q L + + F+LN ++R+LS I G+ + L L ST LF +RE+
Sbjct: 9 HAARLASSGLLL---QVLFRLITFVLNAFILRFLSKEIVGVVNVRLTLLYSTTLFLAREA 65
Query: 66 IRISTLRIKSTTDSGKLEKVEDGEDTRTLQSLINFGYIPFVIGLPLSIILISWQYSNLNS 125
R + L G R +N ++ +G+ S+ L W + L
Sbjct: 66 FRRACL---------------SGGTQRDWSQTLNLLWLTVPLGVFWSLFL-GWIWLQLLE 109
Query: 126 YFID--LPYFKASIFLIWLSILIELVSEPFYLVHQYLLNHFIRSKYESLGVTFACVANFI 183
+P++ + L LS ++EL+ EPF+++ Q + ++ ESL V V
Sbjct: 110 VPDPNVVPHYATGVVLFGLSAVVELLGEPFWVLAQAHMFVKLKVIAESLSVILKSVLTAF 169
Query: 184 IVVWFEKMVNGVGLELHDDYKQEGIAIFAFAVGKLVHAMTLLACYSYNYYSEVYTTGERY 243
+V+W ++ F++ +L + L+ CY Y++++ + E
Sbjct: 170 LVLWLPHW-----------------GLYIFSLAQLFYTTVLVLCYVI-YFTKLLGSPEST 211
Query: 244 SY------KLTKIRPETRQESYYFQNDTVQHFKKVYFQLCFKHLLTEGDKLIINSLCTVE 297
++T + P + + + + Q K +LTEG++ ++ L +
Sbjct: 212 KLQTLPVSRITDLLPNITRNGAFINWKEAKLTWSFFKQSFLKQILTEGERYVMTFLNVLN 271
Query: 298 --EQGIYSLLSNYGSLITRLLFAPIXXXXXXXXXXX------XSVSSKKNLWLSMKVLID 349
+QG+Y +++N GSL+ RL+F PI ++ ++++ ++ VL
Sbjct: 272 FGDQGVYDIVNNLGSLVARLIFQPIEESFYIFFAKVLERGKDATLQKQEDVAVAAAVLES 331
Query: 350 LTKFYLYLSLFIIIFGPINSSYLLKFVIGSKWSSTSFLETIRTYCFYIPFLSLNGIFEAF 409
L K L L I +FG S L G+ SS S +R+YC Y+ L++NG+ E F
Sbjct: 332 LLKLALLAGLTITVFGFAYSQLALDIYGGTMLSSGSGPVLLRSYCLYVLLLAINGVTECF 391
Query: 410 FQSVASGDQIFKHSYVMMLFSGIFLFNCWLFIEYFDLSLEGLIVSNILNMALRIAYCGNF 469
+ S +++ ++++VM+ S FL +L + G I++N NM +RI F
Sbjct: 392 TFAAMSKEEVDRYNFVMLALSSSFLVLSYLLTRW--CGSVGFILANCFNMGIRITQSLCF 449
Query: 470 IHKFY 474
IH++Y
Sbjct: 450 IHRYY 454
>At5g07630 [D] KOG2864 Nuclear division RFT1 protein
Length = 401
Score = 86.3 bits (212), Expect = 1e-16
Identities = 90/384 (23%), Positives = 167/384 (43%), Gaps = 55/384 (14%)
Query: 85 VEDG-EDTRTLQSLINFGYIPFVIGLPLSI---ILISWQYSNLNSYFIDLPYFKASIFLI 140
+ DG E + ++ ++ +G+ ++I I + W + NL+ + D PY +A I++
Sbjct: 6 IPDGPESEENVTRILKVAWVTVPLGIAITIAACIFVLW-WQNLS--YSD-PYAQA-IWIH 60
Query: 141 WLSILIELVSEPFYLVHQYLLNHFIRSKYESLGVTFACVANFIIVVWFEKMVNGVGLELH 200
+ ++EL++EP Y++ Q LL +R E++ CV + ++V M
Sbjct: 61 GFACVLELMAEPLYILSQTLLKLQLRLIVETVATLARCVTLYSLLVMQTNMEK------- 113
Query: 201 DDYKQEGIAIFAFAVGKLVHAMTLLACYSYNYYSEVYTTGERYSYKLTKIRPETRQESYY 260
+ FA+ ++ + +L Y++ G S L RP +
Sbjct: 114 ---------VIIFALSQVAYGGSLFI----GYWAYFLICGVYRSSNLFPFRPGNFMD--- 157
Query: 261 FQNDTVQHFKKVYFQLCFKHLLTEGDKLIINSLCTVEEQGIYSLLSNYGSLITRLLFAPI 320
F ND + K FQ K +L E +L GSL+ R++F P
Sbjct: 158 FDNDLSKMCKLFTFQSFRKLILQE-------------------VLKTPGSLVVRMVFLPF 198
Query: 321 XXXXXXXXXXXXSVS-SKKNLWLSMKVLIDLTKFYLYLSLFIIIFGPINSSYLLKFVIGS 379
S ++N L + + + L K + + L + FGP S L++ + G
Sbjct: 199 EESSYTIFARFASGDYQERNKKLGIYLTVAL-KLVILIGLIFMAFGPSYSYSLIRLLYGE 257
Query: 380 KWSSTSFLETIRTYCFYIPFLSLNGIFEAFFQSVASGDQIFKHSYVMMLFSGIFLFNCWL 439
KWS ++ YC YI L++NG EAF +V + +++ + + ++++FS I++ L
Sbjct: 258 KWSDGEASLALQFYCLYIIVLAMNGTSEAFLHAVGTKNELERSNDMLLIFSLIYVALNIL 317
Query: 440 FIEYFDLSLEGLIVSNILNMALRI 463
I GLI++N L+ + I
Sbjct: 318 LIR--SAGAIGLIMANSLSWQILI 339
>7290615 [D] KOG2864 Nuclear division RFT1 protein
Length = 556
Score = 86.3 bits (212), Expect = 1e-16
Identities = 119/506 (23%), Positives = 206/506 (40%), Gaps = 75/506 (14%)
Query: 14 GVLFLMLGQTLSKGVNFLLNTLLVRYLSPRIFGITSFLEFLL-STVLFFSRESIRISTLR 72
G F ++ Q L + + F +N +VR++ + GI + LL ST+LF SRE+I + L
Sbjct: 12 GAGFSIIFQILCRILTFGINAYIVRHVGREVLGIMNVRLLLLESTLLFLSREAINRAALS 71
Query: 73 IKSTTDSGKLEKVEDGEDTRTLQSLINFGYIPF----VIGLPLSIILISWQYSNLNSYFI 128
+ D + LIN ++ V+ P I ++W S +++ +
Sbjct: 72 ANAQQG-----------DRCSWAQLINQMWLTVPICAVLCAPCLYIWLNW-LSAVDAIYA 119
Query: 129 DLPYFKASIFLIWLSILIELVSEPFYLVHQYLLNHFIRSKYESLGVTFACVANFIIVVWF 188
++ + + + S ++EL++E V Q F K + L T + I +W
Sbjct: 120 S--QYEFACYAVAFSCVLELMAESAVFVAQV----FCFVKLKILLNTLHILVRSAIFLW- 172
Query: 189 EKMVNGVGLELHDDYKQEGIAIFAFAVGKLVHAMTL-LACYSYNY-YSEVYTTGERYSYK 246
+V G AI AFA+ +L A+T+ L Y + Y Y + + K
Sbjct: 173 --IVTG----------DRSAAINAFAIAQLSSAVTIVLGQYGFFYFYLKGFKDFVTQQAK 220
Query: 247 LTKIRPETRQESYYFQNDTV------------------QHFKKVYFQLCF--------KH 280
+ P+ Q S Y D +HF + L K
Sbjct: 221 KKPVAPKAWQVSLYEHMDDFPFKQLSDFLPGVMFNPNGKHFNRELQTLTLSFVKQGVLKQ 280
Query: 281 LLTEGDKLI--INSLCTVEEQGIYSLLSNYGSLITRLLFAPIXXXXXXXXXXXXSVSSK- 337
+LTEG+K + ++ + + EQ Y +++N GS+ R +F PI S K
Sbjct: 281 ILTEGEKYVMSVSPVLSFGEQATYDVVNNLGSMAARFIFRPIEDSSYFYFTQTLSRDIKL 340
Query: 338 -----KNLWLSMKVLIDLTKFYLYLSLFIIIFGPINSSYLLKFVIGSKWSSTSFLETI-R 391
+ + + VL +L + L FG S +L G + + +++ +
Sbjct: 341 AKQPQERVRQASSVLNNLLLGVSSIGLIAFTFGQSYSYPVLLLYGGPDFVAGGLPQSLLQ 400
Query: 392 TYCFYIPFLSLNGIFEAFFQSVASGDQIFKHSYVMMLFSGIFLFNCWLFIEYFDLSLEGL 451
+C I L++NGI E + + + I K++Y+M +FS FL ++ F G
Sbjct: 401 WHCLAIYLLAVNGISEGYMFATNTSRDIDKYNYLMAIFSVSFLVLSYILTGIF--GPVGF 458
Query: 452 IVSNILNMALRIAYCGNFIHKFYHYL 477
I +N +NM RI Y +I Y L
Sbjct: 459 IFANCINMLSRILYSTYYIRHQYRPL 484
>CE07621 [D] KOG2864 Nuclear division RFT1 protein
Length = 522
Score = 83.6 bits (205), Expect = 7e-16
Identities = 114/505 (22%), Positives = 209/505 (40%), Gaps = 72/505 (14%)
Query: 21 GQTLSKGVNFLLNTLLVRYLSPRIFGITSF-LEFLLSTVLFFSRESIRISTLRIKSTTDS 79
GQ +++ ++F +N L+R ++ + G+ + L L S++LF +RE +R K+
Sbjct: 13 GQLIARIISFAINMYLLRRINNDVLGLVNVRLTLLYSSILFLTREPLR------KAEIIR 66
Query: 80 GKLEKVEDGEDTRTLQSLINFGYIPFVIGLPLSIILISWQYSNLNSYFIDLPYFKASIFL 139
G L K IN ++ +I +S++ + Y+ +S ++ + S+ L
Sbjct: 67 GSLPK------------FINLLWLSPIISTVISVVCVYLWYA-FSSTSDEVSW---SVLL 110
Query: 140 IW-LSILIELVSEPFYLVHQYLLNHFIRSKYESLGVTFACVANFIIVVWFEKMVNGVGLE 198
+ +S +IE ++EPF ++ L SK SL FA +I V +++ GL
Sbjct: 111 SFPISAIIESIAEPFSVISLRL-----ESKCGSLAQHFAIGQGMLICV--KRIFVLAGLF 163
Query: 199 LHDDYKQEGIAIFAFAVGKLVHAMTLLACYSYNYYSEVYTTGERYSYKLTKIRPETRQES 258
+ + +A +G + + + + Y Y K PE Q S
Sbjct: 164 MFPGMYHLELFAYAQYIGAIAYLLFNFVAF--------------YIYIRNKSIPELEQFS 209
Query: 259 YY----------FQNDTVQHFKKVYFQLCFKHLLTEGDKLIIN--SLCTVEEQGIYSLLS 306
+ D++ ++ K LLT+G ++ L ++++Q +Y +
Sbjct: 210 TFSDLFPKFSEGIDRDSIHAVFTMFSHSILKQLLTDGSAYVMTFTELLSLKDQAVYDAVE 269
Query: 307 NYGSLITRLLFAPIXXXXXXXXXXXXSVSSK---KNLWLSMKVLIDLTKFYLYLSLFIII 363
GS+I R + +PI S KN ++ L+K + + +
Sbjct: 270 RVGSIIVRTILSPIDENCNAYFSNTIRKESSVFNKNTDNHDDLVDTLSKVLHVVGVIGFV 329
Query: 364 ---FGPINSSYLLKFVIGSKWSSTSFLETIRTYCFYIPFLSLNGIFEAFFQSVASGDQIF 420
FG S ++ G S + Y YI ++NGI E F + QIF
Sbjct: 330 ACTFGIPYSPVVISLYGGKLLSENGGALLLSLYSGYILVTAINGITEGFAMASMDNRQIF 389
Query: 421 KHSYVMMLFSGIFLFNCWLFIEYFDLSLEGLIVSNILNMALRIAYCGNFIHKFYHYLLKE 480
H + + S I L ++ Y ++ G IV+NI+NM++RI Y I ++ L +
Sbjct: 390 THGKFLFVTSIIHLIINYVLCVY--MNSAGFIVANIINMSVRIIYNWRTIREY----LGD 443
Query: 481 SSTETTQSILP--NISTFKNVALIA 503
T+ +LP S F V+L+A
Sbjct: 444 KCPSFTE-VLPTGQTSIFLGVSLLA 467
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.327 0.142 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,201,604
Number of Sequences: 60738
Number of extensions: 1178670
Number of successful extensions: 3506
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3484
Number of HSP's gapped (non-prelim): 6
length of query: 556
length of database: 30,389,216
effective HSP length: 111
effective length of query: 445
effective length of database: 23,647,298
effective search space: 10523047610
effective search space used: 10523047610
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)