ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2679 good J KOG3082 Translation, ribosomal structure and biogenesis Methionyl-tRNA formyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2679 935421 934324 -366
(366 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBL013w [J] KOG3082 Methionyl-tRNA formyltransferase 232 7e-61
SPAC1805.09c [J] KOG3082 Methionyl-tRNA formyltransferase 88 2e-17
Hs21245130 [J] KOG3082 Methionyl-tRNA formyltransferase 85 1e-16
7302035 [J] KOG3082 Methionyl-tRNA formyltransferase 74 4e-13
CE05817 [F] KOG2452 Formyltetrahydrofolate dehydrogenase 58 3e-08
At1g66520 [J] KOG3082 Methionyl-tRNA formyltransferase 50 7e-06
>YBL013w [J] KOG3082 Methionyl-tRNA formyltransferase
Length = 393
Score = 232 bits (591), Expect = 7e-61
Identities = 148/366 (40%), Positives = 200/366 (54%), Gaps = 36/366 (9%)
Query: 25 PLKVLFFGSDQYXXXXXXXXXXXXRTKD----IDSLQVVTRSPKSCGRYLSEVREVPIMS 80
PL VLFFGSD + + +D++QVVTRSPK CGR S ++ PI
Sbjct: 29 PLNVLFFGSDTFSNFSLQALNELRQNNGSCGIVDNIQVVTRSPKWCGRQKSILKYPPIFD 88
Query: 81 VNDSLGLPPVIKCDTRSDLLGLI------------DNPSVEFNVLIAVSFGKLIPKQLIE 128
+ + L LP I CDT+ ++L L D FN +IAVSFGKLIP LI
Sbjct: 89 MAEKLQLPRPITCDTKQEMLALSKLTPSRQGNPENDGSGAPFNAIIAVSFGKLIPGDLIR 148
Query: 129 KVDGKAFNIHPSLLPRYRGSSPIQYTLLNRDEFTGVTIQSLHPTKFDHGEIIKQTAPLSV 188
V A N+HPSLLPR++GS+PIQ LL D +TGVTIQ+LHP +FDHG I+ QT PL++
Sbjct: 149 AVP-LALNVHPSLLPRHKGSAPIQRALLEGDTYTGVTIQTLHPDRFDHGAIVAQTEPLAI 207
Query: 189 QEILKLGTVGKF-----DEDVPEKVATLMDQLGLKSGELLQQMIRERDFKPKPNPN---- 239
+L G V E +P + A LMDQLG +LL Q +RER + P+
Sbjct: 208 ATMLSKGRVNDSTADFNSEGLPRRTAILMDQLGALGAQLLGQTLRERLYLPQNRVQAPTA 267
Query: 240 YEPSLAPKITTEMKQINWKNERKLRLLAKNDALGSLYCYKLSLPKRK--KEVMKKRIIFP 297
Y+PS A +ITTE K+I+W + LL K + LG L+ +K + RK + + KRI+F
Sbjct: 268 YKPSYAHRITTEDKRIHWARDSAAELLNKLETLGPLHAFKEATAARKDAQNSVLKRILFH 327
Query: 298 E---LFDLNETISMKPGEFKLSEDEKTMIIQCIDG-QIGTNVLQFEGFALEPVPTFVNRL 353
E + D KPG FK + + +++ C + + LQFEGFA+E F+
Sbjct: 328 ECKVMRDARLDNGSKPGMFKYDDIKDCILVTCRGNLLLCVSRLQFEGFAVERAGQFM--- 384
Query: 354 NKRCGK 359
RCGK
Sbjct: 385 -ARCGK 389
>SPAC1805.09c [J] KOG3082 Methionyl-tRNA formyltransferase
Length = 340
Score = 88.2 bits (217), Expect = 2e-17
Identities = 67/244 (27%), Positives = 116/244 (47%), Gaps = 53/244 (21%)
Query: 114 IAVSFGKLIPKQLIEKVDGKAFNIHPSLLPRYRGSSPIQYTLLNRDEFTGVTIQSLHPTK 173
I SFG+ +P +++ ++ NIHPSLLP+YRG+ P+ T+LN D GVTIQ++ +
Sbjct: 84 ITASFGRFVPFKILNQLPYGGINIHPSLLPKYRGAGPVYSTILNGDRLAGVTIQTMDSKQ 143
Query: 174 FDHGEII---------KQTAPLSVQEILKLGTVGKFDEDV---------------PEKVA 209
FD G+ + K+T L + +IL LG G + + P+
Sbjct: 144 FDKGKSLAQAYLKLNGKETYTL-LTKILSLGAAGMLEHVLLQSLYLPNCQTNTVAPQIHG 202
Query: 210 TLMDQLGLKSGELLQQMIRERDFKPKPNPNYEPSLAPKITTEMKQINWKNERKLRLLAKN 269
+ D GL Q+I + F P+++ S A KIT E +N+++ ++ +
Sbjct: 203 RITDTGGL-------QLISKETF-----PSFQESWAKKITPEDAHVNFESMNTQQIYNMS 250
Query: 270 DALGSLYCYKLSLPKRKKEVMKKRIIFPELFDLNET-----ISMKPGEFKLSEDEKTMII 324
A ++C + K++ ++ L+ T I MKPGEF L E + +++
Sbjct: 251 RAFNHVWCI----------LNNKKVFLYDVHPLHSTSAEDWIHMKPGEFALLE-KNLLLV 299
Query: 325 QCID 328
+ +D
Sbjct: 300 KTLD 303
>Hs21245130 [J] KOG3082 Methionyl-tRNA formyltransferase
Length = 304
Score = 85.1 bits (209), Expect = 1e-16
Identities = 63/239 (26%), Positives = 113/239 (46%), Gaps = 30/239 (12%)
Query: 104 DNPSVEFNVLIAVSFGKLIPKQLIEKVDGKAFNIHPSLLPRYRGSSPIQYTLLNRDEFTG 163
D S E++V + SFG+L+ + LI K N+HPS LPR+RG +P+ +T+L+ D TG
Sbjct: 27 DVGSGEYDVGVVASFGRLLNEALILKFPYGILNVHPSCLPRWRGPAPVIHTVLHGDTVTG 86
Query: 164 VTIQSLHPTKFDHGEIIKQ-TAPLSVQEILKLGTVGKFDEDVPEKVATLMDQLGLKSGEL 222
VTI + P +FD G I+KQ T P+ + K ++ ++ +LG
Sbjct: 87 VTIMQIRPKRFDVGPILKQETVPVPPKSTAK-------------ELEAVLSRLGANMLIS 133
Query: 223 LQQMIRERDFKPKPNPNYEPSLAPKITTEMKQINWKNERKLRLLAKNDALGSLYCYKLSL 282
+ + + E + P + APKI+ I W+ + ++ A+G++ +
Sbjct: 134 VLKNLPESLSNGRQQPMEGATYAPKISAGTSCIKWEEQTSEQIFRLYRAIGNI------I 187
Query: 283 PKRKKEVMKKRIIFPELFDLNETI---------SMKPGEFKLSEDEKTMIIQCIDGQIG 332
P + M I +L ++N ++ ++ PG + + +++ C DG IG
Sbjct: 188 PLQTL-WMANTIKLLDLVEVNSSVLADPKLTGQALIPGSVIYHKQSQILLVYCKDGWIG 245
>7302035 [J] KOG3082 Methionyl-tRNA formyltransferase
Length = 220
Score = 73.6 bits (179), Expect = 4e-13
Identities = 62/231 (26%), Positives = 105/231 (44%), Gaps = 21/231 (9%)
Query: 136 NIHPSLLPRYRGSSPIQYTLLNRDEFTGVTIQSLHPTKFDHGEIIKQTAPLSVQEILKLG 195
N+H SLLP++RG++PI Y ++ D TGV+I + P +FD G+I+ Q ++
Sbjct: 3 NVHASLLPKWRGAAPIIYAIMKGDASTGVSIMKIEPHRFDIGDILAQREVAINPDV---- 58
Query: 196 TVGKFDEDVPEKVATLMDQLGLKSGELLQQMIRERDFKPKPNPNYEPSLAPKITTEMKQI 255
F D+ +A+L +L + + L ++E KP + S APKIT+++ +I
Sbjct: 59 ----FMPDLHASLASLGAELLVDTINNLSVRLKE----AKPQDSSRVSYAPKITSKITEI 110
Query: 256 NWKNERKLRLLAKNDALGSLYCYK-LSLPKRKKEVMKKRIIFPELFDLNETISMKPGEFK 314
W + + ++ A LY YK L+ K+V + PE +PG
Sbjct: 111 IWSELSAIEIYTRHRA---LYGYKNLTTNFLGKQVQLLELRLPE----KRVPVQEPGAIS 163
Query: 315 LSEDEKTMIIQCI-DGQIGTNVLQFEGFALEPVPTFVNRLNKRCGKMNSNE 364
+++II C Q+ L+ EG F N K+ ++ E
Sbjct: 164 YLRKSRSLIIGCAQQSQLEVLQLRVEGRKPMSAQDFNNGFLKQARSLSFTE 214
>CE05817 [F] KOG2452 Formyltetrahydrofolate dehydrogenase
Length = 887
Score = 57.8 bits (138), Expect = 3e-08
Identities = 56/177 (31%), Positives = 87/177 (48%), Gaps = 13/177 (7%)
Query: 88 PPVIKCDTRSDLLGLIDNPSVEFNVLIAVSFGKLIPKQLIEKVDGKAFNIHPSLLPRYRG 147
P K +T ++L L + E NVL + + IP ++ E K+ HPS+LP++RG
Sbjct: 64 PETGKFETLPEMLELYKSFGAELNVLPFCT--QFIPLEITEAPAKKSIIYHPSILPKHRG 121
Query: 148 SSPIQYTLLNRDEFTGVTIQSLHPTKFDHGEIIKQTAPLSVQEILKLGTVGKFDEDVPEK 207
+S I +TL+ DE G++I D G I+ Q V+E L T+ K P
Sbjct: 122 ASAINWTLIEGDEEAGLSI-FWADDGLDTGPILLQ-KKCKVEENDTLNTLYK-RFLYPAG 178
Query: 208 VATLMDQLGLKSGELLQQMIRERDFKPKPNPNYEPSLAPKITTEMKQINW-KNERKL 263
VA + + S EL+ R +P+ +YEP + K E+ QI+W K +R+L
Sbjct: 179 VAAVAE-----SVELIATGKAPRIVQPEEGASYEPYITTK--PELAQIDWSKTQRQL 228
>At1g66520 [J] KOG3082 Methionyl-tRNA formyltransferase
Length = 355
Score = 49.7 bits (117), Expect = 7e-06
Identities = 40/148 (27%), Positives = 69/148 (46%), Gaps = 13/148 (8%)
Query: 114 IAVSFGKLIPKQLIEKVDGKAFNIHPSLLPRYRGSSPIQYTLLNRDEFTGVTIQSLHPTK 173
I ++G ++P + ++ NIHPSLLP YRG++P+Q L + TGV++ + K
Sbjct: 116 ITAAYGNILPTKFLKIPVHGTVNIHPSLLPLYRGAAPVQRALQDGVPETGVSL-AFTVRK 174
Query: 174 FDHGEIIKQTAPLSVQEILKLGTVGKFDEDVPEKVATLMDQ-LGLKSGELLQQMIRERDF 232
D G +I + V +++K PE ++ L + L EL
Sbjct: 175 LDAGPVI-ASKRFQVDDLIK----------APELLSFLFSEGSNLLIRELPSIFDGSAKS 223
Query: 233 KPKPNPNYEPSLAPKITTEMKQINWKNE 260
K P + + +LAPKI + +++ E
Sbjct: 224 KAAPQDDSKATLAPKIAPDEAWLSFDQE 251
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,113,140
Number of Sequences: 60738
Number of extensions: 962829
Number of successful extensions: 2277
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2262
Number of HSP's gapped (non-prelim): 7
length of query: 366
length of database: 30,389,216
effective HSP length: 108
effective length of query: 258
effective length of database: 23,829,512
effective search space: 6148014096
effective search space used: 6148014096
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)