ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2746 good S KOG2895 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2746 955310 954087 -408
(408 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR149w [S] KOG2895 Uncharacterized conserved protein 467 e-131
SPBC776.05 [S] KOG2895 Uncharacterized conserved protein 228 1e-59
At5g35460 [S] KOG2895 Uncharacterized conserved protein 114 3e-25
>YGR149w [S] KOG2895 Uncharacterized conserved protein
Length = 432
Score = 467 bits (1201), Expect = e-131
Identities = 223/386 (57%), Positives = 287/386 (73%), Gaps = 18/386 (4%)
Query: 3 DQQSFRTRLATFIEFLDPIASTVQS--YSQPSKQFIKQKLTN----GKQKLQFRDQEISP 56
D+ + L + +EFLDPIAS V S Y +QKL N ++K P
Sbjct: 11 DETNNSVSLTSLLEFLDPIASKVVSKYYHGSHLSKAEQKLRNFEGFRRRKPHHEHDSHHP 70
Query: 57 ----------QLANFKQKTLKKLKTIDKPLQSIFFQNSSTLEKSFYPFTLFNIFLIGFLI 106
QL +FK + L++++ +DKPL SIFF+NSS LEK+FYPFTLFNIF IGFL+
Sbjct: 71 HHLNRSRSFLQLEDFKVRALQRIRNLDKPLDSIFFKNSSRLEKAFYPFTLFNIFFIGFLM 130
Query: 107 GRYPQYFHIYYTAMLCLLMPVRFYTYYKTNNHYFLADLCYFVNAMCLLFIWVLPSSTHLY 166
GR+P++FH+YYT + +LMP+RFYTYYKT NHYFLAD CYFVN +CLLFIW+ P S L+
Sbjct: 131 GRFPEWFHVYYTILFFVLMPIRFYTYYKTKNHYFLADFCYFVNMLCLLFIWIFPYSYSLF 190
Query: 167 QSCFAFTFGTLSFAIITWRNSLVIHSVDKITSCFIHIMPPLTLYCITHAITEQFKLQRFP 226
QSCFAFTFGTL FA+ITWRNSLVIHS+DK TSCFIHI+PP +Y I H + ++K++RFP
Sbjct: 191 QSCFAFTFGTLCFAVITWRNSLVIHSIDKTTSCFIHIIPPCVMYVIYHGLPLEYKIERFP 250
Query: 227 AAAMKNSKSWNLKTNVFYTSIYYLIWQSLYHYFITLRKKQKIKSGQRMTSFEYLTSHQYK 286
A +++ ++K N+ +TS+YYL+WQSLYHYFITL+K KIKSG+RMTSFEYLT+HQ+K
Sbjct: 251 GAIIQS--ELDIKKNILWTSLYYLVWQSLYHYFITLKKSSKIKSGERMTSFEYLTTHQFK 308
Query: 287 NSWVVKLPPPQPMILYTLFQYCYQLVTMILCVIWLNNSIAAAGFLIFIFLHAAKNGASYY 346
N W VKL P PMI+YTL QY YQL TM+LC IW+ +AAA FL +FL A+ NGA+YY
Sbjct: 309 NFWAVKLRSPWPMIIYTLSQYFYQLFTMLLCGIWIRYKLAAALFLTIVFLWASHNGATYY 368
Query: 347 VDYYGKKFEKEVDKLRIQVETLEKQL 372
+D+YGK FEKEVD+LR++VE L+++L
Sbjct: 369 IDHYGKNFEKEVDRLRLEVENLQQKL 394
>SPBC776.05 [S] KOG2895 Uncharacterized conserved protein
Length = 404
Score = 228 bits (581), Expect = 1e-59
Identities = 118/345 (34%), Positives = 198/345 (57%), Gaps = 16/345 (4%)
Query: 28 YSQPSKQFIKQKLTNGKQKLQFRDQEISPQLANFKQKTLKKLKTIDKPLQS---IFFQNS 84
Y+Q Q +K ++ K KLQ ++I+ + K++ K + + S + F+
Sbjct: 54 YNQKKGQ-LKHGVSRQKNKLQPIHKQINYETDKLKERLGKSIDKFQEQWNSGKVVRFR-- 110
Query: 85 STLEKSFYPFTLFNIFLIGFLIGRYPQYFHIYYTAMLCLLMPVRFYTYYKTNNHYFLADL 144
+K + + L L+G P+ H++YT L + +P+R+YTY + YF+AD
Sbjct: 111 ---DKLSFALGVSTCILTALLVGMAPESMHLWYTIQLFVYLPLRYYTYQRKGYEYFIADF 167
Query: 145 CYFVNAMCLLFIWVLPSSTHLYQSCFAFTFGTLSFAIITWRNSLVIHSVDKITSCFIHIM 204
CY+ N + L++IW+ P S L+ ++ ++GTL+++++ WRNSL+ HS+DKITS FIH
Sbjct: 168 CYWGNILLLVYIWIFPESRRLFILSYSISYGTLAWSVVAWRNSLLFHSIDKITSLFIHFF 227
Query: 205 PPLTLYCITHAITEQFKLQRFPAAAMKNSKSWNLKTNVFYTSIYYLIWQSLYHYFITLRK 264
PPL L+ I H + + RFP A+ K +L ++V S +Y +WQ Y++FI + K
Sbjct: 228 PPLVLHTIVHLTNKSYLKDRFP--AVLKVKKIDLLSSVEIASFFYALWQIWYYFFIQVGK 285
Query: 265 KQKIKSGQRMTSFEYLTSHQYKNS----WVVKLPPPQPMILYTLFQYCYQLVTMILCVIW 320
+++I+ G R TSF +L+ Y + V KLP ++ + QY Y + TM+ C +W
Sbjct: 286 QKQIQEG-RPTSFTWLSKASYSKTKLGRAVAKLPQNLQPFVFMIIQYLYSITTMLPCSLW 344
Query: 321 LNNSIAAAGFLIFIFLHAAKNGASYYVDYYGKKFEKEVDKLRIQV 365
NN + + FL IF + NGASYY+D +G++F+KE++ LR Q+
Sbjct: 345 YNNKLYSTAFLALIFGWSVWNGASYYIDVFGRRFQKELEALRQQL 389
>At5g35460 [S] KOG2895 Uncharacterized conserved protein
Length = 381
Score = 114 bits (285), Expect = 3e-25
Identities = 79/282 (28%), Positives = 136/282 (48%), Gaps = 14/282 (4%)
Query: 104 FLIGRYPQYFHIYYTAMLCLLMPVRFYTYYKTNNHYFLADLCYFVNAMCLLFIWVLPSST 163
FL+G PQ + Y + +P+R+ Y HY+L D CY+ N + L+ + + P +
Sbjct: 82 FLLGARPQDIPLVYCFFYVIFVPLRWIYYRFKKWHYYLLDFCYYANTIFLVDLLLYPKNE 141
Query: 164 HLYQSCFAFTFGTLSFAIITWRNSLVIHSVDKITSCFIHIMPPLTLYCI------THAIT 217
L+ CF+F G L++AII WR SLV S DKI S IH++P L + I T A
Sbjct: 142 KLFMVCFSFAEGPLAWAIIVWRCSLVFSSPDKIVSVLIHLLPGLVFFTIRWWNPATFAAM 201
Query: 218 EQFKLQRFPAAAMKNSKSWNLKTNVFYTSIYYLIWQSLYHYFI-TLRKKQKIKSGQRMTS 276
R + K++ + Y +WQ LY + LR+++ ++ + MTS
Sbjct: 202 HPVGTDRRVSWPYVEDKAYLFTWLFLVPLVVYTLWQVLYFLIVNVLRRQRLLRDPEVMTS 261
Query: 277 FEYLTSHQYKNS---WVVK--LPPPQPMILYTLFQYCYQLVTMILCV-IWLNNSIAAAGF 330
+ L+ K + W + L + +Y LFQ + + TM L V I+L+ + F
Sbjct: 262 YRELSKKAEKANNKLWQLSGLLGDQNRIWMYILFQAIFTVATMALTVPIFLSYRLHVI-F 320
Query: 331 LIFIFLHAAKNGASYYVDYYGKKFEKEVDKLRIQVETLEKQL 372
I A NG S+ ++ ++ ++ K + +++ +E+Q+
Sbjct: 321 QILKISAAVWNGGSFLLEVMPRQVIQKEKKKKAEMQPIEEQI 362
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.327 0.138 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,269,754
Number of Sequences: 60738
Number of extensions: 1015222
Number of successful extensions: 3268
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3256
Number of HSP's gapped (non-prelim): 3
length of query: 408
length of database: 30,389,216
effective HSP length: 109
effective length of query: 299
effective length of database: 23,768,774
effective search space: 7106863426
effective search space used: 7106863426
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)