ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV2747 good J KOG2988 Translation, ribosomal structure and biogenesis 60S ribosomal protein L30
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV2747 gi|730563|sp|P38664|RL30_KLULA 60S RIBOSOMAL
PROTEIN L30 (L32)
(111 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL030w [J] KOG2988 60S ribosomal protein L30 188 1e-48
SPAC1250.05 [J] KOG2988 60S ribosomal protein L30 140 3e-34
SPAC9G1.03c [J] KOG2988 60S ribosomal protein L30 137 2e-33
CE20413 [J] KOG2988 60S ribosomal protein L30 134 3e-32
7298519 [J] KOG2988 60S ribosomal protein L30 133 5e-32
Hs4506631 [J] KOG2988 60S ribosomal protein L30 127 2e-30
At3g18740 [J] KOG2988 60S ribosomal protein L30 124 3e-29
At1g36240 [J] KOG2988 60S ribosomal protein L30 122 6e-29
Hs20551686 [J] KOG2988 60S ribosomal protein L30 111 2e-25
At1g77940 [J] KOG2988 60S ribosomal protein L30 111 2e-25
Hs17435581 [J] KOG2988 60S ribosomal protein L30 87 3e-18
ECU05g1490 [J] KOG2988 60S ribosomal protein L30 71 2e-13
Hs20544602 [J] KOG2988 60S ribosomal protein L30 42 1e-04
CE00455 [J] KOG2988 60S ribosomal protein L30 37 0.004
Hs4759034 [J] KOG0688 Peptide chain release factor 1 (eRF1) 31 0.32
CE29530 [R] KOG1516 Carboxylesterase and related proteins 28 2.7
At3g26618 [J] KOG0688 Peptide chain release factor 1 (eRF1) 27 4.6
YDL208w [A] KOG3167 Box H/ACA snoRNP component involved in ribos... 27 6.1
At1g12920 [J] KOG0688 Peptide chain release factor 1 (eRF1) 27 6.1
7290340 [T] KOG0902 Phosphatidylinositol 4-kinase 27 6.1
>YGL030w [J] KOG2988 60S ribosomal protein L30
Length = 105
Score = 188 bits (477), Expect = 1e-48
Identities = 95/104 (91%), Positives = 102/104 (97%)
Query: 8 APAKNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAM 67
AP K+QE+INQKLALV+KSGKY+LGYKSTVKSLRQGK+KLIIIAANTPVLRKSELEYYAM
Sbjct: 2 APVKSQESINQKLALVIKSGKYTLGYKSTVKSLRQGKSKLIIIAANTPVLRKSELEYYAM 61
Query: 68 LSKTKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTALA 111
LSKTKVYYFQGGNNELGTAVGKLFRVGVV+IL+AGDSDILT LA
Sbjct: 62 LSKTKVYYFQGGNNELGTAVGKLFRVGVVSILEAGDSDILTTLA 105
>SPAC1250.05 [J] KOG2988 60S ribosomal protein L30
Length = 117
Score = 140 bits (353), Expect = 3e-34
Identities = 69/99 (69%), Positives = 83/99 (83%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ + IN KLAL +KSGKY LGYKST+K+LR GKAKLI+IAAN P LRKSELEYYAMLS+
Sbjct: 19 KSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRKSELEYYAMLSR 78
Query: 71 TKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTA 109
V+++ G N +LGTA GKLFRVGV+ ++DAGDSDIL A
Sbjct: 79 CSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDILAA 117
>SPAC9G1.03c [J] KOG2988 60S ribosomal protein L30
Length = 109
Score = 137 bits (345), Expect = 2e-33
Identities = 70/99 (70%), Positives = 81/99 (81%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ + IN KLAL +KSGKY LGYKST+K+LR GKAKLI+IA N P LRKSELEYYAMLSK
Sbjct: 11 KSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELEYYAMLSK 70
Query: 71 TKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTA 109
V+++ G N +LGTA GKLFRVGV+ I DAGDSDIL A
Sbjct: 71 ANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDILDA 109
>CE20413 [J] KOG2988 60S ribosomal protein L30
Length = 113
Score = 134 bits (336), Expect = 3e-32
Identities = 64/102 (62%), Positives = 83/102 (80%)
Query: 9 PAKNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAML 68
P KN ENIN +L++V+K+G+Y LGYK T+KSL GKAKL+IIA NTP LRKSE+EYYAML
Sbjct: 7 PQKNAENINSRLSMVMKTGQYVLGYKQTLKSLLNGKAKLVIIANNTPPLRKSEIEYYAML 66
Query: 69 SKTKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTAL 110
+KT V+++ G N ELGTA G+LFRV + + DAGDSDI+ ++
Sbjct: 67 AKTGVHHYNGNNIELGTACGRLFRVCTLAVTDAGDSDIILSV 108
>7298519 [J] KOG2988 60S ribosomal protein L30
Length = 111
Score = 133 bits (334), Expect = 5e-32
Identities = 66/108 (61%), Positives = 86/108 (79%)
Query: 3 LLFVQAPAKNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSEL 62
++ V+ K E+ N +LALV+KSGKY LGYK T+K+LRQGKAKL++IA+NTP LRKSE+
Sbjct: 1 MVAVKKQKKALESTNARLALVMKSGKYCLGYKQTLKTLRQGKAKLVLIASNTPALRKSEI 60
Query: 63 EYYAMLSKTKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTAL 110
EYYAML+KT+V ++ G N ELGTA GK FRV ++I D GDSDI+ +L
Sbjct: 61 EYYAMLAKTEVQHYSGTNIELGTACGKYFRVCTLSITDPGDSDIIRSL 108
>Hs4506631 [J] KOG2988 60S ribosomal protein L30
Length = 115
Score = 127 bits (320), Expect = 2e-30
Identities = 61/100 (61%), Positives = 80/100 (80%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ E+IN +L LV+KSGKY LGYK T+K +RQGKAKL+I+A N P LRKSE+EYYAML+K
Sbjct: 9 KSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAK 68
Query: 71 TKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTAL 110
T V+++ G N ELGTA GK +RV + I+D GDSDI+ ++
Sbjct: 69 TGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSM 108
>At3g18740 [J] KOG2988 60S ribosomal protein L30
Length = 112
Score = 124 bits (310), Expect = 3e-29
Identities = 60/100 (60%), Positives = 78/100 (78%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ E IN +LALV+KSGKY+LGYKS +KSLR K KLI+I++N P LR+SE+EYYAML+K
Sbjct: 9 KSHEGINSRLALVMKSGKYTLGYKSVLKSLRSSKGKLILISSNCPPLRRSEIEYYAMLAK 68
Query: 71 TKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTAL 110
V+ + G N +LGTA GK FRV ++I+D GDSDI+ L
Sbjct: 69 VGVHRYNGNNVDLGTACGKYFRVSCLSIVDPGDSDIIKTL 108
>At1g36240 [J] KOG2988 60S ribosomal protein L30
Length = 112
Score = 122 bits (307), Expect = 6e-29
Identities = 59/100 (59%), Positives = 79/100 (79%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ E IN +LALV+KSGKY+LGYKS +KSLR K KLI+I++N P LR+SE+EYYAML+K
Sbjct: 9 KSHEGINSRLALVMKSGKYTLGYKSVLKSLRSSKGKLILISSNCPPLRRSEIEYYAMLAK 68
Query: 71 TKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTAL 110
V+++ N +LGTA GK FRV ++I+D GDSDI+ +L
Sbjct: 69 VGVHHYNRNNVDLGTACGKYFRVSCLSIVDPGDSDIIKSL 108
>Hs20551686 [J] KOG2988 60S ribosomal protein L30
Length = 243
Score = 111 bits (277), Expect = 2e-25
Identities = 60/113 (53%), Positives = 78/113 (68%), Gaps = 5/113 (4%)
Query: 2 LLLFVQAPAKNQEN----INQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVL 57
LLL P K +N IN +L L+ KSGKY LGYK T+K +RQG AKL+I+A N P L
Sbjct: 125 LLLTGAIPTKEMKNSLESINYRLQLM-KSGKYMLGYKQTLKMIRQGTAKLVILAYNCPAL 183
Query: 58 RKSELEYYAMLSKTKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTAL 110
RKSE+EYYAML+K V+++ G N ELGTA GK +RV + + D GDS I+ ++
Sbjct: 184 RKSEVEYYAMLAKIGVHHYSGNNIELGTACGKYYRVCTLALTDPGDSAIIRSM 236
>At1g77940 [J] KOG2988 60S ribosomal protein L30
Length = 308
Score = 111 bits (277), Expect = 2e-25
Identities = 59/122 (48%), Positives = 79/122 (64%), Gaps = 22/122 (18%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ E IN +LALV+KSGKY+LGYKS +KSLR K KLI+I+ N P LR+SE+EYYAML+K
Sbjct: 9 KSHEGINSRLALVMKSGKYTLGYKSVLKSLRGSKGKLILISTNCPPLRRSEIEYYAMLAK 68
Query: 71 TKVYYFQGG----------------------NNELGTAVGKLFRVGVVTILDAGDSDILT 108
V+++ G N +LGTA GK FRV ++I+D GDSDI+
Sbjct: 69 VGVHHYNGSKFLSSLARPVFKDSLFSCFCEDNVDLGTACGKYFRVSCLSIVDPGDSDIIK 128
Query: 109 AL 110
++
Sbjct: 129 SI 130
>Hs17435581 [J] KOG2988 60S ribosomal protein L30
Length = 169
Score = 87.4 bits (215), Expect = 3e-18
Identities = 44/76 (57%), Positives = 54/76 (70%)
Query: 11 KNQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSK 70
K+ E IN +L LV+KSGKY L YK T K +RQGKAKL I N P LRKS++EYY ML+K
Sbjct: 9 KSVEWINSRLLLVIKSGKYILAYKQTPKMIRQGKAKLAIHTENCPALRKSKIEYYTMLTK 68
Query: 71 TKVYYFQGGNNELGTA 86
T V+++ N ELG A
Sbjct: 69 TGVHHYSDNNFELGIA 84
>ECU05g1490 [J] KOG2988 60S ribosomal protein L30
Length = 108
Score = 71.2 bits (173), Expect = 2e-13
Identities = 33/97 (34%), Positives = 62/97 (63%), Gaps = 1/97 (1%)
Query: 14 ENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEYYAMLSKTKV 73
+N+ L L +++GKY +G+K +KS+ KAK ++I++N P ++ LEYY++L+
Sbjct: 9 DNVTSSLLLAMRTGKYIVGFKRAIKSVIMKKAKCLVISSNFPSTKRKLLEYYSVLAGGLP 68
Query: 74 YYFQGGNN-ELGTAVGKLFRVGVVTILDAGDSDILTA 109
F NN ELG+ + R+G ++I+D G+++++ A
Sbjct: 69 IIFHSANNEELGSITERGHRLGCISIIDQGEAELIPA 105
>Hs20544602 [J] KOG2988 60S ribosomal protein L30
Length = 165
Score = 42.0 bits (97), Expect = 1e-04
Identities = 19/34 (55%), Positives = 25/34 (72%)
Query: 67 MLSKTKVYYFQGGNNELGTAVGKLFRVGVVTILD 100
ML+KT V+++ G N ELGTA GK +RVG I+D
Sbjct: 1 MLAKTGVHHYGGNNIELGTACGKYYRVGTPAIID 34
>CE00455 [J] KOG2988 60S ribosomal protein L30
Length = 178
Score = 37.0 bits (84), Expect = 0.004
Identities = 19/38 (50%), Positives = 28/38 (73%), Gaps = 1/38 (2%)
Query: 12 NQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLII 49
N ENIN +L++V+K+G+Y L Y+ +KSL AKL+I
Sbjct: 99 NAENINFRLSMVMKTGQYVL-YEQKLKSLLNENAKLVI 135
>Hs4759034 [J] KOG0688 Peptide chain release factor 1 (eRF1)
Length = 437
Score = 30.8 bits (68), Expect = 0.32
Identities = 11/33 (33%), Positives = 21/33 (63%)
Query: 26 SGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLR 58
+GKY G + T+K+L G +++I+ N ++R
Sbjct: 298 TGKYCFGVEDTLKALEMGAVEILIVYENLDIMR 330
>CE29530 [R] KOG1516 Carboxylesterase and related proteins
Length = 811
Score = 27.7 bits (60), Expect = 2.7
Identities = 19/77 (24%), Positives = 36/77 (46%), Gaps = 5/77 (6%)
Query: 12 NQENINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIA-----ANTPVLRKSELEYYA 66
N++N+ + L + +S+G TV+SL + + + + NTP++ + A
Sbjct: 708 NEKNVAKTLVEIYGDYYFSVGAFDTVRSLVKYGNTVYLYSFNYFNPNTPIVFGKNRPFNA 767
Query: 67 MLSKTKVYYFQGGNNEL 83
+ KVY F G + L
Sbjct: 768 AIQMEKVYRFHGKSTVL 784
>At3g26618 [J] KOG0688 Peptide chain release factor 1 (eRF1)
Length = 435
Score = 26.9 bits (58), Expect = 4.6
Identities = 11/33 (33%), Positives = 19/33 (57%)
Query: 26 SGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLR 58
+GKY G + T+K+L G + +I+ N + R
Sbjct: 298 TGKYVFGVEDTLKALEMGAVETLIVWENLDINR 330
>YDL208w [A] KOG3167 Box H/ACA snoRNP component involved in ribosomal RNA
pseudouridinylation
Length = 173
Score = 26.6 bits (57), Expect = 6.1
Identities = 14/44 (31%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
Query: 14 ENINQKLALVVKSGKYSL----GYKSTVKSLRQGKAKLIIIAAN 53
+ +N+K+ VK + G K VK+LR+G+ L++IA +
Sbjct: 54 KKLNKKVLKTVKKASKAKNVKRGVKEVVKALRKGEKGLVVIAGD 97
>At1g12920 [J] KOG0688 Peptide chain release factor 1 (eRF1)
Length = 434
Score = 26.6 bits (57), Expect = 6.1
Identities = 11/33 (33%), Positives = 19/33 (57%)
Query: 26 SGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLR 58
+GKY G + T+K+L G + +I+ N + R
Sbjct: 297 TGKYVFGVEDTLKALEMGAIETLIVWENLDINR 329
>7290340 [T] KOG0902 Phosphatidylinositol 4-kinase
Length = 2113
Score = 26.6 bits (57), Expect = 6.1
Identities = 20/72 (27%), Positives = 35/72 (47%), Gaps = 7/72 (9%)
Query: 2 LLLFVQAPAKNQENINQKLALVVKSGKYS-------LGYKSTVKSLRQGKAKLIIIAANT 54
L +F+ KN E I+++++ +V S + L Y T K+L Q L+ I + +
Sbjct: 1566 LAVFLPQRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESPDLVYILSWS 1625
Query: 55 PVLRKSELEYYA 66
PV L Y++
Sbjct: 1626 PVTPIQALAYFS 1637
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.356
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,507,209
Number of Sequences: 60738
Number of extensions: 182094
Number of successful extensions: 482
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 463
Number of HSP's gapped (non-prelim): 20
length of query: 111
length of database: 30,389,216
effective HSP length: 87
effective length of query: 24
effective length of database: 25,105,010
effective search space: 602520240
effective search space used: 602520240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)